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The map label for this gene is mcrB [H]

Identifier: 77458885

GI number: 77458885

Start: 3053206

End: 3055242

Strand: Direct

Name: mcrB [H]

Synonym: Pfl01_2660

Alternate gene names: 77458885

Gene position: 3053206-3055242 (Clockwise)

Preceding gene: 77458881

Following gene: 77458886

Centisome position: 47.42

GC content: 53.51

Gene sequence:

>2037_bases
ATGACATCAGTGGCGCCAACCTATTTCACCCAAGACCAATTCCAATGCTTGCGTACCTGGCATGACAGGCCTTTTGACAA
AACCGATGCTTCTCACCGACAAGCCTATCGTCAATTGCGCGAAGCCTATGACATCACCAAGGATTGGGCGTATGCATTGC
AACATCGCGTCTTCCCCGATAGCACAGCCCCCCGCGTTATTCGCAGACCCATAAGCCAGTGGTACAGAAAATTTTTGGGC
TACAACTGGGCCCGTATCTACCCTCGCAGGGATGCTCCCCTGGGGCTGGCTTATACCGTAGGGATTGATGCCACCAAGGG
CTTCGTCGTCAAACTCGATCTGGTCGACACAAAAATTGCAGATGATGGCTTGCGCGAACGTTATGAGAAGTTGCGTGGCC
CTTTTGGAGCTTCACCCATTGTCGCGATCTTGCCGGCTGAAGAAGGCCTGAAGCGAGGCTTTCACGGCATTGTCGAATGG
AGCGCCGCGGCTATTACACACTTTGGAATCGGATACGACGAAATGGCTGACCAACTTGGCTTGACCACTGCACCCACCGA
CGCCGAAATATTGAGGCACTTCGAGGTCAGCAAGGATTTCAAACTGCGGCAACCACAATGGAGCCGCGAGACGACACAGT
TATTTGTGCGCTTGGCCGTCGCGATACATAGAGTGGGTTTTGATTGGTGGTCTACAAAGTCTACTAACGCCCAGTTGGTA
TTCGGGTGGAAGCAGAAGAGCGCGATAAGGGGATCATCGGTCGGAGTGCTGTTCGTGCAAGCGAATCGTGTGTCGGTCAG
GTGGGTCTCATTCGGTGACGTGAGTACGATATCGAAAAACCTGGAACTGAGTGAGGAGCTGGTGGCATCGGTCGAAGCGG
TAAACGCCCAGAAGACTGGTGTCTGGCCCCAGCAAATTCAACAATCCAATCGTCCTGGCTTCTGGCCGGATGATTACAGC
AGCGAAGAAGAAGCCGGCGGGGAGGAGGGCGAGCAGCCAGTTGGCCTCGTATTGACCGTGGGAGCTACCGCCCCACGCAA
TGAAATCTATTTTGGTCCTCCGGGCACTGGCAAGACCCGCACGCTGCAACATTTGCTCAGTGGCAAATACACATCTGCCG
ATGGGATAAAACGCTATGAGTTTGTGACCTTTCACCAGTCCTACGGCTACGAAGAATTCGTTGAAGGCCTGCGCCCGGTG
CTGGTCGATTCAGAGGGGCCGACGGTTGATGGAAACGCTGAATCAGCCGCTTCAAAGGATGTACGATACGAGATCAAAAA
AGGTGCCTTTCTTCGACTGTGCGAAAAGGCCCAGGCCGATCCACACCAGCAATACGCGATGGTGATTGACGAGATCAATC
GCGGCAACATCAGCAAGATCTTCGGTGAGTTGATCACGCTGATCGAAGCCGACAAGCGCAAGGGTGGCGAGTATGAGGTG
ACACTAACATTGCCTTACTCGGGCAAATCGTTCAGCGTCCCCTCAAACGTTGACGTAATCGGCACAATGAACACCGCTGA
TCGTTCGCTGGCGCTAGTTGATACGGCATTGCGCCGGCGCTTTGAGTTCGTCGAATTGATGCCCGATCCTCGTGTGCTGA
CCGAAATCAAGGTTGTCAGTGGAGAGCATGAAATACAGCTTGATCGTTTGCTGACGGCACTTAACGCACGCATCGAGGCG
TTGTATGACCGTGAACATACGATCGGACACGCGTACTTCACACCGTTGAAAAACCTTGTCCCTGAGGCACGTTTCGAGGC
GCTCGTCACCATTTTCCGGACACGTATCCTTCCACTGCTGGAAGAATACTTTTTCGAGGATTGGCAGAAAGTCCGCCTGG
TACTGGGCGATAACCAGAAAACCGACGAAGCGCTTCAGTTTGTCCACGAGCGAAACGCCGACAGGGGTTCCAAAACGCTG
TTCGGCACCCGTGATGATCTGGATGAATACTCGTTGCGGCCTCAATACCGCTTGAATAAGGAGGTGTTTGGTAGCCCGCC
AGCCTATGTCGGTGTCTATGCGACGTTTGCTGAATGA

Upstream 100 bases:

>100_bases
GGTGAGGATTATCTGACGATTAATAAAAGGTGGCGAGACCGCAAAGATCTATCCACACGCCGGCAGTGATGGCACCATGC
GATCACTTTCAAGGGACGGA

Downstream 100 bases:

>100_bases
CGGCGATACAGATTTTCGAGTTCGATACGCTGGTCGCCTCGGATGAAGACTCCAATGGCACGACTGTTCCCCTTAAGGTC
TTCGCTTGGCTGGAAGAGCA

Product: restriction endonuclease GTPase subunit-like protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MTSVAPTYFTQDQFQCLRTWHDRPFDKTDASHRQAYRQLREAYDITKDWAYALQHRVFPDSTAPRVIRRPISQWYRKFLG
YNWARIYPRRDAPLGLAYTVGIDATKGFVVKLDLVDTKIADDGLRERYEKLRGPFGASPIVAILPAEEGLKRGFHGIVEW
SAAAITHFGIGYDEMADQLGLTTAPTDAEILRHFEVSKDFKLRQPQWSRETTQLFVRLAVAIHRVGFDWWSTKSTNAQLV
FGWKQKSAIRGSSVGVLFVQANRVSVRWVSFGDVSTISKNLELSEELVASVEAVNAQKTGVWPQQIQQSNRPGFWPDDYS
SEEEAGGEEGEQPVGLVLTVGATAPRNEIYFGPPGTGKTRTLQHLLSGKYTSADGIKRYEFVTFHQSYGYEEFVEGLRPV
LVDSEGPTVDGNAESAASKDVRYEIKKGAFLRLCEKAQADPHQQYAMVIDEINRGNISKIFGELITLIEADKRKGGEYEV
TLTLPYSGKSFSVPSNVDVIGTMNTADRSLALVDTALRRRFEFVELMPDPRVLTEIKVVSGEHEIQLDRLLTALNARIEA
LYDREHTIGHAYFTPLKNLVPEARFEALVTIFRTRILPLLEEYFFEDWQKVRLVLGDNQKTDEALQFVHERNADRGSKTL
FGTRDDLDEYSLRPQYRLNKEVFGSPPAYVGVYATFAE

Sequences:

>Translated_678_residues
MTSVAPTYFTQDQFQCLRTWHDRPFDKTDASHRQAYRQLREAYDITKDWAYALQHRVFPDSTAPRVIRRPISQWYRKFLG
YNWARIYPRRDAPLGLAYTVGIDATKGFVVKLDLVDTKIADDGLRERYEKLRGPFGASPIVAILPAEEGLKRGFHGIVEW
SAAAITHFGIGYDEMADQLGLTTAPTDAEILRHFEVSKDFKLRQPQWSRETTQLFVRLAVAIHRVGFDWWSTKSTNAQLV
FGWKQKSAIRGSSVGVLFVQANRVSVRWVSFGDVSTISKNLELSEELVASVEAVNAQKTGVWPQQIQQSNRPGFWPDDYS
SEEEAGGEEGEQPVGLVLTVGATAPRNEIYFGPPGTGKTRTLQHLLSGKYTSADGIKRYEFVTFHQSYGYEEFVEGLRPV
LVDSEGPTVDGNAESAASKDVRYEIKKGAFLRLCEKAQADPHQQYAMVIDEINRGNISKIFGELITLIEADKRKGGEYEV
TLTLPYSGKSFSVPSNVDVIGTMNTADRSLALVDTALRRRFEFVELMPDPRVLTEIKVVSGEHEIQLDRLLTALNARIEA
LYDREHTIGHAYFTPLKNLVPEARFEALVTIFRTRILPLLEEYFFEDWQKVRLVLGDNQKTDEALQFVHERNADRGSKTL
FGTRDDLDEYSLRPQYRLNKEVFGSPPAYVGVYATFAE
>Mature_677_residues
TSVAPTYFTQDQFQCLRTWHDRPFDKTDASHRQAYRQLREAYDITKDWAYALQHRVFPDSTAPRVIRRPISQWYRKFLGY
NWARIYPRRDAPLGLAYTVGIDATKGFVVKLDLVDTKIADDGLRERYEKLRGPFGASPIVAILPAEEGLKRGFHGIVEWS
AAAITHFGIGYDEMADQLGLTTAPTDAEILRHFEVSKDFKLRQPQWSRETTQLFVRLAVAIHRVGFDWWSTKSTNAQLVF
GWKQKSAIRGSSVGVLFVQANRVSVRWVSFGDVSTISKNLELSEELVASVEAVNAQKTGVWPQQIQQSNRPGFWPDDYSS
EEEAGGEEGEQPVGLVLTVGATAPRNEIYFGPPGTGKTRTLQHLLSGKYTSADGIKRYEFVTFHQSYGYEEFVEGLRPVL
VDSEGPTVDGNAESAASKDVRYEIKKGAFLRLCEKAQADPHQQYAMVIDEINRGNISKIFGELITLIEADKRKGGEYEVT
LTLPYSGKSFSVPSNVDVIGTMNTADRSLALVDTALRRRFEFVELMPDPRVLTEIKVVSGEHEIQLDRLLTALNARIEAL
YDREHTIGHAYFTPLKNLVPEARFEALVTIFRTRILPLLEEYFFEDWQKVRLVLGDNQKTDEALQFVHERNADRGSKTLF
GTRDDLDEYSLRPQYRLNKEVFGSPPAYVGVYATFAE

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=277, Percent_Identity=38.6281588447653, Blast_Score=162, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 76899; Mature: 76767

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVAPTYFTQDQFQCLRTWHDRPFDKTDASHRQAYRQLREAYDITKDWAYALQHRVFPD
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
STAPRVIRRPISQWYRKFLGYNWARIYPRRDAPLGLAYTVGIDATKGFVVKLDLVDTKIA
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEEECCHHH
DDGLRERYEKLRGPFGASPIVAILPAEEGLKRGFHGIVEWSAAAITHFGIGYDEMADQLG
HHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
LTTAPTDAEILRHFEVSKDFKLRQPQWSRETTQLFVRLAVAIHRVGFDWWSTKSTNAQLV
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
FGWKQKSAIRGSSVGVLFVQANRVSVRWVSFGDVSTISKNLELSEELVASVEAVNAQKTG
EEECCCCCCCCCCEEEEEEEECCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCC
VWPQQIQQSNRPGFWPDDYSSEEEAGGEEGEQPVGLVLTVGATAPRNEIYFGPPGTGKTR
CCHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCHHH
TLQHLLSGKYTSADGIKRYEFVTFHQSYGYEEFVEGLRPVLVDSEGPTVDGNAESAASKD
HHHHHHCCCCCCCCCCCEEEEEEEHHHCCHHHHHHCCCCEEECCCCCCCCCCCHHHHCCC
VRYEIKKGAFLRLCEKAQADPHQQYAMVIDEINRGNISKIFGELITLIEADKRKGGEYEV
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
TLTLPYSGKSFSVPSNVDVIGTMNTADRSLALVDTALRRRFEFVELMPDPRVLTEIKVVS
EEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GEHEIQLDRLLTALNARIEALYDREHTIGHAYFTPLKNLVPEARFEALVTIFRTRILPLL
CCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EEYFFEDWQKVRLVLGDNQKTDEALQFVHERNADRGSKTLFGTRDDLDEYSLRPQYRLNK
HHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCHHHCCCCCHHCCH
EVFGSPPAYVGVYATFAE
HHCCCCCCEEEEEEEECC
>Mature Secondary Structure 
TSVAPTYFTQDQFQCLRTWHDRPFDKTDASHRQAYRQLREAYDITKDWAYALQHRVFPD
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
STAPRVIRRPISQWYRKFLGYNWARIYPRRDAPLGLAYTVGIDATKGFVVKLDLVDTKIA
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEEECCHHH
DDGLRERYEKLRGPFGASPIVAILPAEEGLKRGFHGIVEWSAAAITHFGIGYDEMADQLG
HHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
LTTAPTDAEILRHFEVSKDFKLRQPQWSRETTQLFVRLAVAIHRVGFDWWSTKSTNAQLV
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
FGWKQKSAIRGSSVGVLFVQANRVSVRWVSFGDVSTISKNLELSEELVASVEAVNAQKTG
EEECCCCCCCCCCEEEEEEEECCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCC
VWPQQIQQSNRPGFWPDDYSSEEEAGGEEGEQPVGLVLTVGATAPRNEIYFGPPGTGKTR
CCHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCHHH
TLQHLLSGKYTSADGIKRYEFVTFHQSYGYEEFVEGLRPVLVDSEGPTVDGNAESAASKD
HHHHHHCCCCCCCCCCCEEEEEEEHHHCCHHHHHHCCCCEEECCCCCCCCCCCHHHHCCC
VRYEIKKGAFLRLCEKAQADPHQQYAMVIDEINRGNISKIFGELITLIEADKRKGGEYEV
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
TLTLPYSGKSFSVPSNVDVIGTMNTADRSLALVDTALRRRFEFVELMPDPRVLTEIKVVS
EEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GEHEIQLDRLLTALNARIEALYDREHTIGHAYFTPLKNLVPEARFEALVTIFRTRILPLL
CCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EEYFFEDWQKVRLVLGDNQKTDEALQFVHERNADRGSKTLFGTRDDLDEYSLRPQYRLNK
HHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCHHHCCCCCHHCCH
EVFGSPPAYVGVYATFAE
HHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]