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The map label for this gene is 77457520

Identifier: 77457520

GI number: 77457520

Start: 1468540

End: 1469997

Strand: Direct

Name: 77457520

Synonym: Pfl01_1293

Alternate gene names: NA

Gene position: 1468540-1469997 (Clockwise)

Preceding gene: 77457519

Following gene: 77457521

Centisome position: 22.81

GC content: 66.05

Gene sequence:

>1458_bases
GTGAGTCCGTTAGCCATTGATCCGATTGTCGCCCTCGACGCCGATGCGCTGTCCCGGGCGATCCATGCCCGCAAGGTGTC
GTGCCGCGAGGTGATGCAGGCCTACCTGACGCACATTGATCGCTTCAATCCGGCGGTCAATGCGCTGGTTTCGCTGCGCC
CGCAAGACGTGCTGCTGCGCGAGGCCGATGAATGCGATCGGCAACTGGATCGCAGCGAGTCCCGAGGCTGGATGCACGGC
ATGCCCCAGGCGATCAAGGACCTGGCGGCCACTGCCGGACTGCGCACGACCCTCGGCTCGCCACTGTTCGCCGAGCAAGT
GCCGCAGCACGACGCGATCAGCGTGGCGCGGGTTCGCGACTGCGGCGCGATCATCATCGGCAAAAGCAACGTCCCGGAAT
TCGGCCTCGGTTCGCAGACCTACAACAGCGTGTTCGGCACCACCACCAACGCCTACGATCCGAGCCTGATCGCCGGCGGC
AGCAGCGGCGGGGCGGCGGTGGCGCTGGCCCTGCGCCTGCTGCCGGTGGCCGACGGCAGTGACATGATGGGCTCGCTGCG
CAACCCGGCGGCGTTCAACAACGTGTTCGGCTTCCGCCCGTCCCAGGGCCGCGTGCCTCACGGCCCGATGCCGGAAGTGT
TCGTCCAGCAACTGGCCACCGAAGGGCCTATGGGGCGCACGGTCACCGACGTCGCGCGATTGCTCTCGACCCAGGCCGGC
TACGATCCGCGTGTGCCGTTGTCGCTGGCTGATGAACCACGGGATTTCGGGCAGGATCTGCAACAGGACTGCTGCGGCCT
GCGCCTCGGCTGGCTCGGCGATTACAACGGCTATCTGCCGATGGAAGACGGCGTGTTGAGCCTGTGCGAATCGGCGCTGG
CGGATTTCAGTGCGCTGGGTTGCCACGTCGAGGCCTGTCAGCCGGACTTTTCCATGGAACGCCTGTGGCAGACCTGGCTC
ACCCACCGGCATTTTCTGGTGCAGGGCAGCCTCGGCGCGGCCTATGCCTATCCGCAAAAACGGGCGCTGCTCAAACCCGA
AGCGCAATGGGAAATCGAGGGCGGTCTGCGTCTGACCGCTGCCGAGGTCTATCAGGCGTCAGTCGCGCGCAGTGAGTGGT
ATCGCGCGTTGAGCGAGTTATTCGAGCGTTACGATTTCCTGCTGTTGCCCACCGCCCAAGTGTTCCCTTTTGATGCACAG
CAAGCGTGGCCGCGAGTCGTCGGCGGGCAGACCATGGACACCTATCACCGCTGGATGGAGGTGGTGATCGGCCCGACGCT
GGCGGGGCTGCCGAGCATCAGCGTGCCGGTCGGTTTCAACCCGGCCGGGCTGCCGATGGGCCTGCAAATCATCGGTCCGG
CCCAGGCGGATCGGGCGGTGCTGCAACTGGCCTACGCCCATGAACAGTTGACCCGCTGGGTCGAACGGCGACCGCCGGCA
TGCCTGCAATCGGCCTGA

Upstream 100 bases:

>100_bases
ATGTCTTGTTCGCTTGCCTGCTGACCCTGCTCGGTACTTACTGTTTGCAGGAGGCACCACGCGCGAAGAAATCCGTTGCG
CTCAACCTGGGAGTGAAACC

Downstream 100 bases:

>100_bases
GCGGCCCGCATCTTGCTGCACGAAGTGATCTTTCATCCGCATCCATGGAGGTCGAACACATGGGCAGCAAGGCAGACACC
GGCAGCGTGCGCTCGGTCGA

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSPLAIDPIVALDADALSRAIHARKVSCREVMQAYLTHIDRFNPAVNALVSLRPQDVLLREADECDRQLDRSESRGWMHG
MPQAIKDLAATAGLRTTLGSPLFAEQVPQHDAISVARVRDCGAIIIGKSNVPEFGLGSQTYNSVFGTTTNAYDPSLIAGG
SSGGAAVALALRLLPVADGSDMMGSLRNPAAFNNVFGFRPSQGRVPHGPMPEVFVQQLATEGPMGRTVTDVARLLSTQAG
YDPRVPLSLADEPRDFGQDLQQDCCGLRLGWLGDYNGYLPMEDGVLSLCESALADFSALGCHVEACQPDFSMERLWQTWL
THRHFLVQGSLGAAYAYPQKRALLKPEAQWEIEGGLRLTAAEVYQASVARSEWYRALSELFERYDFLLLPTAQVFPFDAQ
QAWPRVVGGQTMDTYHRWMEVVIGPTLAGLPSISVPVGFNPAGLPMGLQIIGPAQADRAVLQLAYAHEQLTRWVERRPPA
CLQSA

Sequences:

>Translated_485_residues
MSPLAIDPIVALDADALSRAIHARKVSCREVMQAYLTHIDRFNPAVNALVSLRPQDVLLREADECDRQLDRSESRGWMHG
MPQAIKDLAATAGLRTTLGSPLFAEQVPQHDAISVARVRDCGAIIIGKSNVPEFGLGSQTYNSVFGTTTNAYDPSLIAGG
SSGGAAVALALRLLPVADGSDMMGSLRNPAAFNNVFGFRPSQGRVPHGPMPEVFVQQLATEGPMGRTVTDVARLLSTQAG
YDPRVPLSLADEPRDFGQDLQQDCCGLRLGWLGDYNGYLPMEDGVLSLCESALADFSALGCHVEACQPDFSMERLWQTWL
THRHFLVQGSLGAAYAYPQKRALLKPEAQWEIEGGLRLTAAEVYQASVARSEWYRALSELFERYDFLLLPTAQVFPFDAQ
QAWPRVVGGQTMDTYHRWMEVVIGPTLAGLPSISVPVGFNPAGLPMGLQIIGPAQADRAVLQLAYAHEQLTRWVERRPPA
CLQSA
>Mature_484_residues
SPLAIDPIVALDADALSRAIHARKVSCREVMQAYLTHIDRFNPAVNALVSLRPQDVLLREADECDRQLDRSESRGWMHGM
PQAIKDLAATAGLRTTLGSPLFAEQVPQHDAISVARVRDCGAIIIGKSNVPEFGLGSQTYNSVFGTTTNAYDPSLIAGGS
SGGAAVALALRLLPVADGSDMMGSLRNPAAFNNVFGFRPSQGRVPHGPMPEVFVQQLATEGPMGRTVTDVARLLSTQAGY
DPRVPLSLADEPRDFGQDLQQDCCGLRLGWLGDYNGYLPMEDGVLSLCESALADFSALGCHVEACQPDFSMERLWQTWLT
HRHFLVQGSLGAAYAYPQKRALLKPEAQWEIEGGLRLTAAEVYQASVARSEWYRALSELFERYDFLLLPTAQVFPFDAQQ
AWPRVVGGQTMDTYHRWMEVVIGPTLAGLPSISVPVGFNPAGLPMGLQIIGPAQADRAVLQLAYAHEQLTRWVERRPPAC
LQSA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=26.6932270916335, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI222831590, Length=517, Percent_Identity=27.0793036750484, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI166795287, Length=271, Percent_Identity=26.5682656826568, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=465, Percent_Identity=28.3870967741935, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17537465, Length=522, Percent_Identity=22.9885057471264, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=289, Percent_Identity=27.3356401384083, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=205, Percent_Identity=30.2439024390244, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=306, Percent_Identity=26.4705882352941, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=306, Percent_Identity=26.4705882352941, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=435, Percent_Identity=28.2758620689655, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=439, Percent_Identity=25.5125284738041, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=226, Percent_Identity=29.646017699115, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=510, Percent_Identity=25.4901960784314, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648435, Length=510, Percent_Identity=25.4901960784314, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648437, Length=510, Percent_Identity=25.4901960784314, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652985, Length=249, Percent_Identity=32.5301204819277, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922090, Length=249, Percent_Identity=32.5301204819277, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24652981, Length=249, Percent_Identity=32.5301204819277, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24652983, Length=249, Percent_Identity=32.5301204819277, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24644968, Length=515, Percent_Identity=23.6893203883495, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356731, Length=231, Percent_Identity=32.4675324675325, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648113, Length=205, Percent_Identity=36.0975609756098, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648441, Length=429, Percent_Identity=26.1072261072261, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=429, Percent_Identity=26.1072261072261, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 52885; Mature: 52754

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLAIDPIVALDADALSRAIHARKVSCREVMQAYLTHIDRFNPAVNALVSLRPQDVLLR
CCCCCCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
EADECDRQLDRSESRGWMHGMPQAIKDLAATAGLRTTLGSPLFAEQVPQHDAISVARVRD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHCCCHHHHHCCCCCCHHHHHCCC
CGAIIIGKSNVPEFGLGSQTYNSVFGTTTNAYDPSLIAGGSSGGAAVALALRLLPVADGS
CCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCH
DMMGSLRNPAAFNNVFGFRPSQGRVPHGPMPEVFVQQLATEGPMGRTVTDVARLLSTQAG
HHHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
YDPRVPLSLADEPRDFGQDLQQDCCGLRLGWLGDYNGYLPMEDGVLSLCESALADFSALG
CCCCCCCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
CHVEACQPDFSMERLWQTWLTHRHFLVQGSLGAAYAYPQKRALLKPEAQWEIEGGLRLTA
CCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHCCCCHHCCCCCCCCEEECCCEEEHH
AEVYQASVARSEWYRALSELFERYDFLLLPTAQVFPFDAQQAWPRVVGGQTMDTYHRWME
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
VVIGPTLAGLPSISVPVGFNPAGLPMGLQIIGPAQADRAVLQLAYAHEQLTRWVERRPPA
HHHCCHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCH
CLQSA
HHCCC
>Mature Secondary Structure 
SPLAIDPIVALDADALSRAIHARKVSCREVMQAYLTHIDRFNPAVNALVSLRPQDVLLR
CCCCCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
EADECDRQLDRSESRGWMHGMPQAIKDLAATAGLRTTLGSPLFAEQVPQHDAISVARVRD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHCCCHHHHHCCCCCCHHHHHCCC
CGAIIIGKSNVPEFGLGSQTYNSVFGTTTNAYDPSLIAGGSSGGAAVALALRLLPVADGS
CCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCH
DMMGSLRNPAAFNNVFGFRPSQGRVPHGPMPEVFVQQLATEGPMGRTVTDVARLLSTQAG
HHHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
YDPRVPLSLADEPRDFGQDLQQDCCGLRLGWLGDYNGYLPMEDGVLSLCESALADFSALG
CCCCCCCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
CHVEACQPDFSMERLWQTWLTHRHFLVQGSLGAAYAYPQKRALLKPEAQWEIEGGLRLTA
CCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHCCCCHHCCCCCCCCEEECCCEEEHH
AEVYQASVARSEWYRALSELFERYDFLLLPTAQVFPFDAQQAWPRVVGGQTMDTYHRWME
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
VVIGPTLAGLPSISVPVGFNPAGLPMGLQIIGPAQADRAVLQLAYAHEQLTRWVERRPPA
HHHCCHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCH
CLQSA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]