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The map label for this gene is ispF [H]

Identifier: 77457354

GI number: 77457354

Start: 1304276

End: 1304749

Strand: Direct

Name: ispF [H]

Synonym: Pfl01_1127

Alternate gene names: 77457354

Gene position: 1304276-1304749 (Clockwise)

Preceding gene: 77457353

Following gene: 77457355

Centisome position: 20.26

GC content: 60.97

Gene sequence:

>474_bases
ATGCGTATTGGCCACGGCTATGATGTGCACCGTTTCGCTGAAGGCGATTTCATTACTCTGGGCGGCGTGCGAATCGCACA
CGGCTTCGGGCTGCTCGCTCACTCCGACGGTGACGTCCTGCTGCACGCCTTGAGCGATGCCTTGCTCGGCGCGGCGGCGC
TGGGTGATATCGGCAAGCACTTCCCGGACACCGACCCGCAATTCAAGGGTGCCGACAGCCGTGTCCTGCTGCGCCATGTG
GTCAGTCTGATCCACGCCAAAGGCTGGAAGGTCGGCAACGTCGACAACACCATCGTGGCCCAGGCTCCGAAAATGGCCCC
CCATATCGAATCGATGCGCGCGCTGATTGCCGCGGATCTGCAAGTAGAACTGGATCAAGTGAACGTGAAAGCCACCACCA
CCGAAAAGCTCGGCTTCGTCGGTCGCGAAGAAGGCATCGCCGTGCATTCCGTTGCCTTGTTGCTGCGCGCATGA

Upstream 100 bases:

>100_bases
ATCGCCAGCTTCATTGACGACCACTTGCAGCATCACGCGCGCGCTCTGAAGAGTTAATGCAGGTAGAATCACGCCCTGAC
AAAATTCGGGGCGTTTTTTT

Downstream 100 bases:

>100_bases
ACGAACTGCAACTGCTCGGCCCGCGGGCCTATGGCGACGCCCTCGGCACCGCGGTACTGAAAGCCATCGCTGAAGATTTC
CAGGTCGATGAAGTCCTCGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGHGYDVHRFAEGDFITLGGVRIAHGFGLLAHSDGDVLLHALSDALLGAAALGDIGKHFPDTDPQFKGADSRVLLRHV
VSLIHAKGWKVGNVDNTIVAQAPKMAPHIESMRALIAADLQVELDQVNVKATTTEKLGFVGREEGIAVHSVALLLRA

Sequences:

>Translated_157_residues
MRIGHGYDVHRFAEGDFITLGGVRIAHGFGLLAHSDGDVLLHALSDALLGAAALGDIGKHFPDTDPQFKGADSRVLLRHV
VSLIHAKGWKVGNVDNTIVAQAPKMAPHIESMRALIAADLQVELDQVNVKATTTEKLGFVGREEGIAVHSVALLLRA
>Mature_157_residues
MRIGHGYDVHRFAEGDFITLGGVRIAHGFGLLAHSDGDVLLHALSDALLGAAALGDIGKHFPDTDPQFKGADSRVLLRHV
VSLIHAKGWKVGNVDNTIVAQAPKMAPHIESMRALIAADLQVELDQVNVKATTTEKLGFVGREEGIAVHSVALLLRA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=70.0636942675159, Blast_Score=206, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 16737; Mature: 16737

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGYDVHRFAEGDFITLGGVRIAHGFGLLAHSDGDVLLHALSDALLGAAALGDIGKH
CCCCCCCCCEEECCCCEEEECCEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
FPDTDPQFKGADSRVLLRHVVSLIHAKGWKVGNVDNTIVAQAPKMAPHIESMRALIAADL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHC
QVELDQVNVKATTTEKLGFVGREEGIAVHSVALLLRA
EEEEEEEEEEEECHHHHCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRIGHGYDVHRFAEGDFITLGGVRIAHGFGLLAHSDGDVLLHALSDALLGAAALGDIGKH
CCCCCCCCCEEECCCCEEEECCEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
FPDTDPQFKGADSRVLLRHVVSLIHAKGWKVGNVDNTIVAQAPKMAPHIESMRALIAADL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHC
QVELDQVNVKATTTEKLGFVGREEGIAVHSVALLLRA
EEEEEEEEEEEECHHHHCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA