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The map label for this gene is truD

Identifier: 77457355

GI number: 77457355

Start: 1304746

End: 1305804

Strand: Direct

Name: truD

Synonym: Pfl01_1128

Alternate gene names: 77457355

Gene position: 1304746-1305804 (Clockwise)

Preceding gene: 77457354

Following gene: 77457356

Centisome position: 20.27

GC content: 62.89

Gene sequence:

>1059_bases
ATGAACGAACTGCAACTGCTCGGCCCGCGGGCCTATGGCGACGCCCTCGGCACCGCGGTACTGAAAGCCATCGCTGAAGA
TTTCCAGGTCGATGAAGTCCTCGATATTCCGTTCAGCGGTGACGGCGAGCACCTGTGGATCTGGGTGGAAAAGCGCGGCC
TGAATACCGAAGAGGCTGCACGCCGTATCGCCAAGGCGGCCGGTGTACCGTTGCGTACGGTGAGCTATGCCGGTCTGAAA
GACCGTCAGGCGCTGACCCGTCAGTGGTTCAGCGTGCAATTGCCGGGCAAGGCTGATCCGGATCTGTCGGCGGCGGAAAA
CGACACGCTGAAAATCCTCAAGACCACTCGCCACAAGCGCAAGCTGCAACGCGGTGCGCATTCGGCAAACGGTTTCACCC
TGCGCCTGACCCAGTTCAATGGCGACAAGGCGGCCATCGACGAGCGCCTGCAACTGATCGCCAAACAAGGTATTCCGAAT
TACTTCGGCGCCCAGCGTTTCGGCCATGATGGCGGTAACGTCGTCGATGCCCGTTCGTGGGCCGCGCGCAAGGCACTGCC
GGAGCAGCGCAATGTGCGCTCGCGCCTGCTGTCGACCGCGCGCAGTTTTCTGTTCAATCAGGTGCTCGCGGCGCGGGTGG
CCGATGGCACCTGGCAGCGCGCCCAGGTCGGCGATCTGCTGGCCTTCACCGACAGCCGCAGCTTTTTTCCGGCTGGTGAA
GCCGAGTGCAGCGATCCGCGTCTGGCCATTCTTGACCTGCACCCGACCGGTCCGCAGTGGGGCGAAGGTGACTCGCCGGC
GGCCGGGGTTGTCCATGATCTGGAGCAGGGGATTGCCGCACGCGAGGCGGATCTGCGCGATTGGTTGATTAATGCCGGTA
TGAGCCACGAACGTCGCATCCTGCGGCTGCCCATTGGCGGGTTGACGTGGCATTATCCCGAGCCTGACATTCTGCAACTG
GAATTCGTCCTCCCGGCCGGATGCTTCGCCACCGTATTGGTGCGCGAGCTCGTTGATCTGGTGCCGGTGGGGCAGACGGA
CAGCCCATGCGTATTCTGA

Upstream 100 bases:

>100_bases
TGGATCAAGTGAACGTGAAAGCCACCACCACCGAAAAGCTCGGCTTCGTCGGTCGCGAAGAAGGCATCGCCGTGCATTCC
GTTGCCTTGTTGCTGCGCGC

Downstream 100 bases:

>100_bases
TTTCTAACGACGATGGGGTGACTGCGCCCGGTCTTGCCGCGCTATATGCTGCGCTGGCGGATTACACCGAGTGCGTGGTT
ATCGCCCCGGAACAGGACAA

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNELQLLGPRAYGDALGTAVLKAIAEDFQVDEVLDIPFSGDGEHLWIWVEKRGLNTEEAARRIAKAAGVPLRTVSYAGLK
DRQALTRQWFSVQLPGKADPDLSAAENDTLKILKTTRHKRKLQRGAHSANGFTLRLTQFNGDKAAIDERLQLIAKQGIPN
YFGAQRFGHDGGNVVDARSWAARKALPEQRNVRSRLLSTARSFLFNQVLAARVADGTWQRAQVGDLLAFTDSRSFFPAGE
AECSDPRLAILDLHPTGPQWGEGDSPAAGVVHDLEQGIAAREADLRDWLINAGMSHERRILRLPIGGLTWHYPEPDILQL
EFVLPAGCFATVLVRELVDLVPVGQTDSPCVF

Sequences:

>Translated_352_residues
MNELQLLGPRAYGDALGTAVLKAIAEDFQVDEVLDIPFSGDGEHLWIWVEKRGLNTEEAARRIAKAAGVPLRTVSYAGLK
DRQALTRQWFSVQLPGKADPDLSAAENDTLKILKTTRHKRKLQRGAHSANGFTLRLTQFNGDKAAIDERLQLIAKQGIPN
YFGAQRFGHDGGNVVDARSWAARKALPEQRNVRSRLLSTARSFLFNQVLAARVADGTWQRAQVGDLLAFTDSRSFFPAGE
AECSDPRLAILDLHPTGPQWGEGDSPAAGVVHDLEQGIAAREADLRDWLINAGMSHERRILRLPIGGLTWHYPEPDILQL
EFVLPAGCFATVLVRELVDLVPVGQTDSPCVF
>Mature_352_residues
MNELQLLGPRAYGDALGTAVLKAIAEDFQVDEVLDIPFSGDGEHLWIWVEKRGLNTEEAARRIAKAAGVPLRTVSYAGLK
DRQALTRQWFSVQLPGKADPDLSAAENDTLKILKTTRHKRKLQRGAHSANGFTLRLTQFNGDKAAIDERLQLIAKQGIPN
YFGAQRFGHDGGNVVDARSWAARKALPEQRNVRSRLLSTARSFLFNQVLAARVADGTWQRAQVGDLLAFTDSRSFFPAGE
AECSDPRLAILDLHPTGPQWGEGDSPAAGVVHDLEQGIAAREADLRDWLINAGMSHERRILRLPIGGLTWHYPEPDILQL
EFVLPAGCFATVLVRELVDLVPVGQTDSPCVF

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Escherichia coli, GI1789102, Length=335, Percent_Identity=37.910447761194, Blast_Score=200, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_PSEPF (Q3KH85)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346860.1
- ProteinModelPortal:   Q3KH85
- SMR:   Q3KH85
- STRING:   Q3KH85
- GeneID:   3716552
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1128
- eggNOG:   COG0585
- HOGENOM:   HBG541221
- OMA:   ALTRQWF
- ProtClustDB:   PRK00984
- BioCyc:   PFLU205922:PFL_1128-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 38769; Mature: 38769

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELQLLGPRAYGDALGTAVLKAIAEDFQVDEVLDIPFSGDGEHLWIWVEKRGLNTEEAA
CCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCCHHHHH
RRIAKAAGVPLRTVSYAGLKDRQALTRQWFSVQLPGKADPDLSAAENDTLKILKTTRHKR
HHHHHHCCCCEEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
KLQRGAHSANGFTLRLTQFNGDKAAIDERLQLIAKQGIPNYFGAQRFGHDGGNVVDARSW
HHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEECHHHH
AARKALPEQRNVRSRLLSTARSFLFNQVLAARVADGTWQRAQVGDLLAFTDSRSFFPAGE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCC
AECSDPRLAILDLHPTGPQWGEGDSPAAGVVHDLEQGIAAREADLRDWLINAGMSHERRI
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LRLPIGGLTWHYPEPDILQLEFVLPAGCFATVLVRELVDLVPVGQTDSPCVF
EEECCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNELQLLGPRAYGDALGTAVLKAIAEDFQVDEVLDIPFSGDGEHLWIWVEKRGLNTEEAA
CCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCCHHHHH
RRIAKAAGVPLRTVSYAGLKDRQALTRQWFSVQLPGKADPDLSAAENDTLKILKTTRHKR
HHHHHHCCCCEEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
KLQRGAHSANGFTLRLTQFNGDKAAIDERLQLIAKQGIPNYFGAQRFGHDGGNVVDARSW
HHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEECHHHH
AARKALPEQRNVRSRLLSTARSFLFNQVLAARVADGTWQRAQVGDLLAFTDSRSFFPAGE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCC
AECSDPRLAILDLHPTGPQWGEGDSPAAGVVHDLEQGIAAREADLRDWLINAGMSHERRI
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LRLPIGGLTWHYPEPDILQLEFVLPAGCFATVLVRELVDLVPVGQTDSPCVF
EEECCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA