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The map label for this gene is kdsA

Identifier: 77457347

GI number: 77457347

Start: 1297764

End: 1298609

Strand: Direct

Name: kdsA

Synonym: Pfl01_1120

Alternate gene names: 77457347

Gene position: 1297764-1298609 (Clockwise)

Preceding gene: 77457346

Following gene: 77457348

Centisome position: 20.16

GC content: 59.69

Gene sequence:

>846_bases
ATGGCACAGAAGATCATCCGCGTCGGCGACATCGAGATTGCCAACGACAAACCCATGGTGCTGTTCGGCGGCATGAACGT
GCTGGAAAGCCGTGACATGGCCATGCAGGTTTGCGAAGAGTACGTGAAGGTCACCGAAAAACTCGGCATCCCTTACGTGT
TCAAGGCCAGCTTCGACAAGGCCAACCGTTCTTCTGTGACCTCCTATCGCGGTCCTGGCCTGGAAGAGGGCATGCGCATC
TTCCAGGACATCAAACAAGCCTTCGGCGTGCCGATCATCACCGACGTCCACGAGCCTGACCAGGCTGCGGTCGTCGCTGA
GGTCTGCGACATCATCCAGCTGCCGGCCTTCCTGTCGCGCCAGACCGATCTGGTCGTCGCGATGGCCAAGACCAACGCTG
TGATCAACATCAAGAAAGCCCAGTTCCTCGCGCCTCAGGAAATGAAACACATCCTGAACAAGTGCGTGGAAGCGGGTAAC
GATCAGTTGATCCTCTGCGAGCGCGGTTCGAGCTTCGGCTACAACAACCTCGTGGTCGACATGCTCGGCTTCGGCATCAT
GAAGCAGTTCGAGTACCCGGTATTCTTCGACGTGACCCATTCGCTGCAAATGCCGGGCGGTCGTTCCGACTCCGCCGGCG
GTCGCCGCGCCCAGGTCACCGACCTGGCCAAGGCTGGCATGAGCCAGTCGCTGGCGGGGCTGTTCCTCGAAGCGCACCCG
GACCCGGACAACGCCAAATGCGACGGCCCTTGCGCCTTGCGTCTGGACAAACTGGAGCCATTCCTGGCCCAGCTCAAAGC
CCTGGACGAACTGGTGAAGAGTTTTCCGACGGTAGAAACCGCGTAA

Upstream 100 bases:

>100_bases
TCCACCCAGAGTTCACCTCGACGCCACGCGATGGCCATCCGCTGTTCAGCGGCTTCGTCAAAGCGGCATTGGCTCAACAC
CAGAAGAAGGCGTAACCCCG

Downstream 100 bases:

>100_bases
ACCTCAATTCTCCGGTAAAGTACCGCACGATTTGTCGCTCATGCCCTCGGGTATGAGCGTTGTCGTCTGCAAGCTGCCCG
CTGCACACCACTTGCCGACG

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MAQKIIRVGDIEIANDKPMVLFGGMNVLESRDMAMQVCEEYVKVTEKLGIPYVFKASFDKANRSSVTSYRGPGLEEGMRI
FQDIKQAFGVPIITDVHEPDQAAVVAEVCDIIQLPAFLSRQTDLVVAMAKTNAVINIKKAQFLAPQEMKHILNKCVEAGN
DQLILCERGSSFGYNNLVVDMLGFGIMKQFEYPVFFDVTHSLQMPGGRSDSAGGRRAQVTDLAKAGMSQSLAGLFLEAHP
DPDNAKCDGPCALRLDKLEPFLAQLKALDELVKSFPTVETA

Sequences:

>Translated_281_residues
MAQKIIRVGDIEIANDKPMVLFGGMNVLESRDMAMQVCEEYVKVTEKLGIPYVFKASFDKANRSSVTSYRGPGLEEGMRI
FQDIKQAFGVPIITDVHEPDQAAVVAEVCDIIQLPAFLSRQTDLVVAMAKTNAVINIKKAQFLAPQEMKHILNKCVEAGN
DQLILCERGSSFGYNNLVVDMLGFGIMKQFEYPVFFDVTHSLQMPGGRSDSAGGRRAQVTDLAKAGMSQSLAGLFLEAHP
DPDNAKCDGPCALRLDKLEPFLAQLKALDELVKSFPTVETA
>Mature_280_residues
AQKIIRVGDIEIANDKPMVLFGGMNVLESRDMAMQVCEEYVKVTEKLGIPYVFKASFDKANRSSVTSYRGPGLEEGMRIF
QDIKQAFGVPIITDVHEPDQAAVVAEVCDIIQLPAFLSRQTDLVVAMAKTNAVINIKKAQFLAPQEMKHILNKCVEAGND
QLILCERGSSFGYNNLVVDMLGFGIMKQFEYPVFFDVTHSLQMPGGRSDSAGGRRAQVTDLAKAGMSQSLAGLFLEAHPD
PDNAKCDGPCALRLDKLEPFLAQLKALDELVKSFPTVETA

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=283, Percent_Identity=67.8445229681979, Blast_Score=403, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_PSEPF (Q3KH93)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346852.1
- HSSP:   O66496
- ProteinModelPortal:   Q3KH93
- SMR:   Q3KH93
- STRING:   Q3KH93
- GeneID:   3716544
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1120
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   EMKHILN
- ProtClustDB:   PRK05198
- BioCyc:   PFLU205922:PFL_1120-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30821; Mature: 30690

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKIIRVGDIEIANDKPMVLFGGMNVLESRDMAMQVCEEYVKVTEKLGIPYVFKASFDK
CCCCEEEECCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSVTSYRGPGLEEGMRIFQDIKQAFGVPIITDVHEPDQAAVVAEVCDIIQLPAFLSR
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCC
QTDLVVAMAKTNAVINIKKAQFLAPQEMKHILNKCVEAGNDQLILCERGSSFGYNNLVVD
CCCEEEEEECCCEEEEEHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MLGFGIMKQFEYPVFFDVTHSLQMPGGRSDSAGGRRAQVTDLAKAGMSQSLAGLFLEAHP
HHHHHHHHHCCCCEEEEEHHHEECCCCCCCCCCCCCHHHHHHHHHCHHHHHHHEEEEECC
DPDNAKCDGPCALRLDKLEPFLAQLKALDELVKSFPTVETA
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQKIIRVGDIEIANDKPMVLFGGMNVLESRDMAMQVCEEYVKVTEKLGIPYVFKASFDK
CCCEEEECCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSVTSYRGPGLEEGMRIFQDIKQAFGVPIITDVHEPDQAAVVAEVCDIIQLPAFLSR
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCC
QTDLVVAMAKTNAVINIKKAQFLAPQEMKHILNKCVEAGNDQLILCERGSSFGYNNLVVD
CCCEEEEEECCCEEEEEHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MLGFGIMKQFEYPVFFDVTHSLQMPGGRSDSAGGRRAQVTDLAKAGMSQSLAGLFLEAHP
HHHHHHHHHCCCCEEEEEHHHEECCCCCCCCCCCCCHHHHHHHHHCHHHHHHHEEEEECC
DPDNAKCDGPCALRLDKLEPFLAQLKALDELVKSFPTVETA
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA