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The map label for this gene is pyrG [H]

Identifier: 77457346

GI number: 77457346

Start: 1296127

End: 1297758

Strand: Direct

Name: pyrG [H]

Synonym: Pfl01_1119

Alternate gene names: 77457346

Gene position: 1296127-1297758 (Clockwise)

Preceding gene: 77457345

Following gene: 77457347

Centisome position: 20.13

GC content: 60.54

Gene sequence:

>1632_bases
ATGACGCGCTACATATTCGTCACGGGCGGTGTTGTTTCTTCTTTGGGGAAAGGCATTGCATCGGCTTCATTGGCGGCCAT
CCTGGAGGCGCGGGGGCTTAAGGTCACCATGCTGAAGCTGGATCCGTACATCAACGTCGACCCGGGCACCATGAGCCCGT
TCCAGCACGGTGAAGTGTTCGTCACCCACGACGGCGCCGAGACCGACCTGGACCTGGGCCACTACGAGCGGTTCATCCGC
ACGACCATGACCCAGAACAACAACTTCACCACCGGCCGTGTCTACGAGCACGTGCTGCGCAAAGAGCGCCGTGGTGACTA
CCTGGGTGCAACCATCCAGGTGATTCCGCACATCACCGACGAAATCAAGCGTCGCATCATCAAGGGTGCCGGCGACGCCG
ACGTGGCCCTGGTGGAAATCGGCGGTACTGTTGGCGACATCGAGTCGCAACCGTTCCTAGAAGCCATCCGCCAGCTGCGT
GTGGAAGTCGGCTCCAAGCGCGCGATGCTGATGCACCTGACCCTGGTTCCGTACATCGCCACCGCTGGCGAGACCAAGAC
CAAGCCGACCCAGCACTCCGTGAAGGAACTGCGTTCGATCGGCCTGCAGCCAGACGTCCTGATCTGCCGCTCCGACCATC
CGGTGGATGTGTCCTCGCGTCGCAAGATCGCGCTGTTCACCAACGTTGAAGAGCGTGCGGTGATCTCCCTGGAAGACGTC
GACACCATCTACAAGATCCCGGCCGTGCTGCACGCTCAGGGTCTGGACGATTTCGTCGTCGAGCGTTTCGGCCTGCAATG
CAATGGCGCCGACCTGTCCGAGTGGGAAAAGGTCGTTGATGCCAAGCTCAATCCAGAGCACGAAGTCACCATCGCGATGG
TCGGCAAGTACATGGAGCTGCTGGACGCCTACAAGTCGCTGATCGAAGCGATGAGTCACGCCGGCATCACCAACCGCACC
AAGGTCAACCTGCGCTACATCGATTCCGAAGACATCGAAAACCAGGGCACTGCCCTGCTGGAAGGCGTCGACGCGATCCT
CGTACCGGGCGGCTTCGGTCTGCGTGGCGTGGAAGGCAAGATCACCGCCGTTCAATACGCTCGCGAAAACAAGGTTCCAT
ACCTGGGCATCTGCCTGGGCATGCAAGTGGCGGTCATCGAGTTCGCCCGTAACGTGCTGGGCTGGAAAGACGCCAACTCC
ACCGAGTTCGATCGTGCCAGCGGCCATCCGGTTGTCGGTCTGATCACCGAGTGGGAAGACGCCACCGGTGCCGTTGAAGT
GCGTACCGAAGCGTCCGACCTGGGCGGCACCATGCGCCTCGGCGCTCAGGAATGCCTGCTCGAAGCCGGCTCCAAGGTGC
ACGACTGCTACGGCAAGGATGTGATTGTCGAGCGTCACCGTCACCGTTACGAAGTGAACAACAACCTGCTGCCGCAAATC
ATCGAAGCCGGCCTGAAGATTTCCGGTCGCTCTTCCGATGCAGCGCTGGTTGAAGTGGTCGAAGCACCGGATCATCCATG
GTTCGTCGCTTGCCAGTTCCACCCAGAGTTCACCTCGACGCCACGCGATGGCCATCCGCTGTTCAGCGGCTTCGTCAAAG
CGGCATTGGCTCAACACCAGAAGAAGGCGTAA

Upstream 100 bases:

>100_bases
AAAGGTGCACTGTGATTAATGCAGGTGATCGGGGGCTTCGGCCTTCCTTCGCTTTCCCCGGCGGCTCGGACCGCTTTAAC
GCAGACTTCTAGGGTTTTTC

Downstream 100 bases:

>100_bases
CCCCGATGGCACAGAAGATCATCCGCGTCGGCGACATCGAGATTGCCAACGACAAACCCATGGTGCTGTTCGGCGGCATG
AACGTGCTGGAAAGCCGTGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFIR
TTMTQNNNFTTGRVYEHVLRKERRGDYLGATIQVIPHITDEIKRRIIKGAGDADVALVEIGGTVGDIESQPFLEAIRQLR
VEVGSKRAMLMHLTLVPYIATAGETKTKPTQHSVKELRSIGLQPDVLICRSDHPVDVSSRRKIALFTNVEERAVISLEDV
DTIYKIPAVLHAQGLDDFVVERFGLQCNGADLSEWEKVVDAKLNPEHEVTIAMVGKYMELLDAYKSLIEAMSHAGITNRT
KVNLRYIDSEDIENQGTALLEGVDAILVPGGFGLRGVEGKITAVQYARENKVPYLGICLGMQVAVIEFARNVLGWKDANS
TEFDRASGHPVVGLITEWEDATGAVEVRTEASDLGGTMRLGAQECLLEAGSKVHDCYGKDVIVERHRHRYEVNNNLLPQI
IEAGLKISGRSSDAALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGFVKAALAQHQKKA

Sequences:

>Translated_543_residues
MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFIR
TTMTQNNNFTTGRVYEHVLRKERRGDYLGATIQVIPHITDEIKRRIIKGAGDADVALVEIGGTVGDIESQPFLEAIRQLR
VEVGSKRAMLMHLTLVPYIATAGETKTKPTQHSVKELRSIGLQPDVLICRSDHPVDVSSRRKIALFTNVEERAVISLEDV
DTIYKIPAVLHAQGLDDFVVERFGLQCNGADLSEWEKVVDAKLNPEHEVTIAMVGKYMELLDAYKSLIEAMSHAGITNRT
KVNLRYIDSEDIENQGTALLEGVDAILVPGGFGLRGVEGKITAVQYARENKVPYLGICLGMQVAVIEFARNVLGWKDANS
TEFDRASGHPVVGLITEWEDATGAVEVRTEASDLGGTMRLGAQECLLEAGSKVHDCYGKDVIVERHRHRYEVNNNLLPQI
IEAGLKISGRSSDAALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGFVKAALAQHQKKA
>Mature_542_residues
TRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFIRT
TMTQNNNFTTGRVYEHVLRKERRGDYLGATIQVIPHITDEIKRRIIKGAGDADVALVEIGGTVGDIESQPFLEAIRQLRV
EVGSKRAMLMHLTLVPYIATAGETKTKPTQHSVKELRSIGLQPDVLICRSDHPVDVSSRRKIALFTNVEERAVISLEDVD
TIYKIPAVLHAQGLDDFVVERFGLQCNGADLSEWEKVVDAKLNPEHEVTIAMVGKYMELLDAYKSLIEAMSHAGITNRTK
VNLRYIDSEDIENQGTALLEGVDAILVPGGFGLRGVEGKITAVQYARENKVPYLGICLGMQVAVIEFARNVLGWKDANST
EFDRASGHPVVGLITEWEDATGAVEVRTEASDLGGTMRLGAQECLLEAGSKVHDCYGKDVIVERHRHRYEVNNNLLPQII
EAGLKISGRSSDAALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGFVKAALAQHQKKA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=44.8214285714286, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI28559085, Length=557, Percent_Identity=43.2675044883303, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI28559083, Length=557, Percent_Identity=43.2675044883303, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI221316689, Length=557, Percent_Identity=43.2675044883303, Blast_Score=485, Evalue=1e-137,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=69.7416974169742, Blast_Score=775, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=616, Percent_Identity=40.0974025974026, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=40.597539543058, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=40.5263157894737, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=558, Percent_Identity=43.5483870967742, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=42.3459244532803, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59699; Mature: 59568

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCCEE
VTHDGAETDLDLGHYERFIRTTMTQNNNFTTGRVYEHVLRKERRGDYLGATIQVIPHITD
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHH
EIKRRIIKGAGDADVALVEIGGTVGDIESQPFLEAIRQLRVEVGSKRAMLMHLTLVPYIA
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
TAGETKTKPTQHSVKELRSIGLQPDVLICRSDHPVDVSSRRKIALFTNVEERAVISLEDV
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEHHH
DTIYKIPAVLHAQGLDDFVVERFGLQCNGADLSEWEKVVDAKLNPEHEVTIAMVGKYMEL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
LDAYKSLIEAMSHAGITNRTKVNLRYIDSEDIENQGTALLEGVDAILVPGGFGLRGVEGK
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCC
ITAVQYARENKVPYLGICLGMQVAVIEFARNVLGWKDANSTEFDRASGHPVVGLITEWED
EEEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCC
ATGAVEVRTEASDLGGTMRLGAQECLLEAGSKVHDCYGKDVIVERHRHRYEVNNNLLPQI
CCCCEEEEECHHHCCCHHHCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHEECCCCHHHHH
IEAGLKISGRSSDAALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGFVKAALAQHQ
HHCCCEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KKA
CCC
>Mature Secondary Structure 
TRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCCEE
VTHDGAETDLDLGHYERFIRTTMTQNNNFTTGRVYEHVLRKERRGDYLGATIQVIPHITD
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHH
EIKRRIIKGAGDADVALVEIGGTVGDIESQPFLEAIRQLRVEVGSKRAMLMHLTLVPYIA
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
TAGETKTKPTQHSVKELRSIGLQPDVLICRSDHPVDVSSRRKIALFTNVEERAVISLEDV
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEHHH
DTIYKIPAVLHAQGLDDFVVERFGLQCNGADLSEWEKVVDAKLNPEHEVTIAMVGKYMEL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
LDAYKSLIEAMSHAGITNRTKVNLRYIDSEDIENQGTALLEGVDAILVPGGFGLRGVEGK
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCC
ITAVQYARENKVPYLGICLGMQVAVIEFARNVLGWKDANSTEFDRASGHPVVGLITEWED
EEEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCC
ATGAVEVRTEASDLGGTMRLGAQECLLEAGSKVHDCYGKDVIVERHRHRYEVNNNLLPQI
CCCCEEEEECHHHCCCHHHCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHEECCCCHHHHH
IEAGLKISGRSSDAALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGFVKAALAQHQ
HHCCCEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA