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The map label for this gene is eno [H]

Identifier: 77457348

GI number: 77457348

Start: 1298766

End: 1300055

Strand: Direct

Name: eno [H]

Synonym: Pfl01_1121

Alternate gene names: 77457348

Gene position: 1298766-1300055 (Clockwise)

Preceding gene: 77457347

Following gene: 77457349

Centisome position: 20.17

GC content: 59.38

Gene sequence:

>1290_bases
ATGGCAAAAATCGTCGACATCAAAGGTCGTGAAGTTCTCGACTCCCGTGGCAATCCCACCGTGGAAGCGGACGTGCTTCT
CGACAACGGCATCATCGGCAGCGCTTGCGCGCCGTCCGGTGCATCCACTGGCTCGCGTGAAGCGCTCGAGCTGCGTGATG
GCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTCAAGGCGGTAGCCAACATCAATGGTCCGATTCGCGATCTGCTGCTG
GGCACCGACCCAAGCGACCAGAAAGCCCTGGATCAGGCGATGATCAAGCTCGACGGTACCGAAAACAAAGCGACCCTGGG
CGCCAACGCGATCCTCGCCGTGTCCCTGGCTGCGGCCAAGGCGGCTGCACAGGATCAGGACCTGCCGCTGTACGCTCACA
TCGCCAATCTGAACGGCACCCCGGGCGTGTACTCGATGCCGGTTCCGATGATGAACATCATCAACGGTGGCGAACACGCC
GACAACAACGTCGACATCCAGGAATTCATGGTTCAGCCGGTTGGCGCCAAGTCCTTCTCGGAAGGTCTGCGCATGGGCAC
CGAGATTTTCCATCACCTCAAAGCCGTGCTGAAGGCCCGTGGCCTGAGCACTGCCGTCGGTGACGAAGGCGGTTTCGCGC
CGAACCTGGCTTCCAACGAAGACGCACTGAAAGTGATCTCCGAAGCAGTCGCCAACGCTGGTTACAAGCTGGGCACCGAC
GTGACCCTGGCTCTGGACTGTGCGGCCAGCGAATTCTTCGAAGACGGCAAGTACAACCTGTCCGGCGAAGGCCAGGTGTT
CACCGCTGAAGGTTTCGCCGACTACCTCAAAGGTCTGACCGAGCGTTACCCGATCATCTCGATCGAAGACGGTCTGGACG
AGTCCGACTGGGCTGGCTGGAAGATCCTCACCGACAAGATCGGCGAGAAGACCCAGCTGGTCGGCGACGACCTGTTCGTG
ACCAACACCAAGATCCTCAAAGAAGGCATCGATAAAAAGATCGCCAACTCGATCCTGATTAAGTTCAACCAGATCGGTAC
CCTGACCGAAACCCTGGAAGCCATCCAGATGGCCAAGGCTGCCGGTTACACCGCCGTGATCTCGCACCGTTCGGGCGAAA
CCGAAGATTCCACCATTGCCGACCTGGCCGTGGGTACTTCGGCTGGCCAGATCAAGACCGGTTCGCTGTGCCGTTCCGAC
CGCGTTTCCAAGTACAACCAACTGCTGCGTATCGAAGAGCAGTTGAATGGCAAGGCCAAGTACAACGGTCGCAGCGAGTT
TCGCGGCTGA

Upstream 100 bases:

>100_bases
GCGTTGTCGTCTGCAAGCTGCCCGCTGCACACCACTTGCCGACGGTAAAAAGATTTCCTCTAGCTGCGTCGTTTTCGTCA
ACTTTGGAGTGTTTACAACA

Downstream 100 bases:

>100_bases
GCAGTAAATGATAGAAAGACACCGGATTGTGTCGGAAAAGTCGTGACAGCGATGGATTTGCCACTAATCTGATGCCTTAT
ATGCACAAGCCTGGATATTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYLGKGVLKAVANINGPIRDLLL
GTDPSDQKALDQAMIKLDGTENKATLGANAILAVSLAAAKAAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHA
DNNVDIQEFMVQPVGAKSFSEGLRMGTEIFHHLKAVLKARGLSTAVGDEGGFAPNLASNEDALKVISEAVANAGYKLGTD
VTLALDCAASEFFEDGKYNLSGEGQVFTAEGFADYLKGLTERYPIISIEDGLDESDWAGWKILTDKIGEKTQLVGDDLFV
TNTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAAGYTAVISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSD
RVSKYNQLLRIEEQLNGKAKYNGRSEFRG

Sequences:

>Translated_429_residues
MAKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYLGKGVLKAVANINGPIRDLLL
GTDPSDQKALDQAMIKLDGTENKATLGANAILAVSLAAAKAAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHA
DNNVDIQEFMVQPVGAKSFSEGLRMGTEIFHHLKAVLKARGLSTAVGDEGGFAPNLASNEDALKVISEAVANAGYKLGTD
VTLALDCAASEFFEDGKYNLSGEGQVFTAEGFADYLKGLTERYPIISIEDGLDESDWAGWKILTDKIGEKTQLVGDDLFV
TNTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAAGYTAVISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSD
RVSKYNQLLRIEEQLNGKAKYNGRSEFRG
>Mature_428_residues
AKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYLGKGVLKAVANINGPIRDLLLG
TDPSDQKALDQAMIKLDGTENKATLGANAILAVSLAAAKAAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHAD
NNVDIQEFMVQPVGAKSFSEGLRMGTEIFHHLKAVLKARGLSTAVGDEGGFAPNLASNEDALKVISEAVANAGYKLGTDV
TLALDCAASEFFEDGKYNLSGEGQVFTAEGFADYLKGLTERYPIISIEDGLDESDWAGWKILTDKIGEKTQLVGDDLFVT
NTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAAGYTAVISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDR
VSKYNQLLRIEEQLNGKAKYNGRSEFRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=52.2041763341067, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=52.2041763341067, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=50.5747126436782, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=50.2304147465438, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=47.0862470862471, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=350, Percent_Identity=29.4285714285714, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI169201757, Length=350, Percent_Identity=29.4285714285714, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI239744207, Length=350, Percent_Identity=29.4285714285714, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI310129182, Length=221, Percent_Identity=27.1493212669683, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310110045, Length=221, Percent_Identity=27.1493212669683, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310120572, Length=221, Percent_Identity=27.1493212669683, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=73.7209302325581, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=52.6436781609195, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=52.6436781609195, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32563855, Length=234, Percent_Identity=45.7264957264957, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=49.1990846681922, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=47.4537037037037, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=47.4537037037037, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=47.4537037037037, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=48.512585812357, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=51.8518518518518, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=51.8518518518518, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=51.8518518518518, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=51.8518518518518, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=51.8518518518518, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=51.8518518518518, Blast_Score=414, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45542; Mature: 45411

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYL
CCEEEECCCHHHHHCCCCCCEEEEEEEECCEECCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVANINGPIRDLLLGTDPSDQKALDQAMIKLDGTENKATLGANAILAVSLAAAK
HHHHHHHHHHCCCCHHHHEECCCCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHH
AAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHADNNVDIQEFMVQPVGAKSFS
HHCCCCCCCEEEEEECCCCCCCEEECCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHH
EGLRMGTEIFHHLKAVLKARGLSTAVGDEGGFAPNLASNEDALKVISEAVANAGYKLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VTLALDCAASEFFEDGKYNLSGEGQVFTAEGFADYLKGLTERYPIISIEDGLDESDWAGW
EEEEEEHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCE
KILTDKIGEKTQLVGDDLFVTNTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKA
EEEHHHCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHH
AGYTAVISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDRVSKYNQLLRIEEQLNGKAK
CCCEEEEECCCCCCCCCHHHHHEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
YNGRSEFRG
CCCHHCCCC
>Mature Secondary Structure 
AKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYL
CEEEECCCHHHHHCCCCCCEEEEEEEECCEECCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVANINGPIRDLLLGTDPSDQKALDQAMIKLDGTENKATLGANAILAVSLAAAK
HHHHHHHHHHCCCCHHHHEECCCCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHH
AAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHADNNVDIQEFMVQPVGAKSFS
HHCCCCCCCEEEEEECCCCCCCEEECCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHH
EGLRMGTEIFHHLKAVLKARGLSTAVGDEGGFAPNLASNEDALKVISEAVANAGYKLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VTLALDCAASEFFEDGKYNLSGEGQVFTAEGFADYLKGLTERYPIISIEDGLDESDWAGW
EEEEEEHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCE
KILTDKIGEKTQLVGDDLFVTNTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKA
EEEHHHCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHH
AGYTAVISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDRVSKYNQLLRIEEQLNGKAK
CCCEEEEECCCCCCCCCHHHHHEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
YNGRSEFRG
CCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA