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The map label for this gene is tilS

Identifier: 77457345

GI number: 77457345

Start: 1294524

End: 1295852

Strand: Direct

Name: tilS

Synonym: Pfl01_1118

Alternate gene names: 77457345

Gene position: 1294524-1295852 (Clockwise)

Preceding gene: 77457344

Following gene: 77457346

Centisome position: 20.11

GC content: 63.66

Gene sequence:

>1329_bases
ATGGGTCAGCCCTCGATTGACTTGCCTTCCAGGCTTCTGCGCAATCTCAAGCCCTGGCTCGGTGCTTCCCATTGGCGCAT
CGCCTTCTCCGGTGGCCTCGATTCCACCGTTCTGCTGCACCTCCTGGCCAAACTCGCCAAAAACCAATCCATTCCGGCGC
TAAGCGCCATCCATGTCCATCACGGTCTTCAGGCTGCGGCCGACGCATGGCCTGCGCATTGTCAGGCTGTTTGCGAGGAA
CTGGCGGTGCCGTTGCAAGTGCAGCGTGTGCAGGTGCAGCCGGGGGCGAGTGTAGAGCGCGCGGCACGGGATGCACGCTA
TGCGGCGTTCAGTGCGGCGACTCAGGCCAATGACGTGCTGCTGACCGGGCAGCATCGCGACGATCAGGCTGAAACGCTGT
TGTTCCGTCTGCTGCGTGGCGCCGGCGTGCGTGGGTTGTCCGGAATGCCGGCCACGCGAGCGCTGGGGCAGGGCAGTCTG
GTGCGGCCGTTGCTCGACGTCACACGCGCAGAGTTGGAAGCCTATGCGCGGGACCATGGCCTGCGTTGGATCGAGGATCC
GTCGAATCAGGATCGACAGTTCTCTCGCAATTATCTGCGCCATCAGATCATGCCGCTGCTGATCGGCCGCTGGCCGCAGG
CACACGCCAGCATGGCCCGCAGCGCGGCGCATCTGCGTGAGGCGCAGGGCTTGCTTGATGAGCTGGCACAAATCGATCTG
ACTCAAGCAAGTTTGCCCAGCGAGTTCGAATGGTCGGGATTGCCATCGCTGGAGTTTGCGGCCATCGCGAAATTGTCCGA
TGCCCGTCAGCGAAATGCACTCAGCCACTGGCTTGAGCCGTTGACCCGACTGCCGGATACCGACCATTGGTCGGGTTGGG
TCGATGTGCGTGACGCCGGCAACGATGCCTCGCCGATCTGGCGTCTGGCGGATGGCGAGCTGCATCGAAGCGCCGGCCGC
TTGTGGTGGCTCAGCGGGGAATGGCTACGCACACCGGTGGTCAGCGGCGACTGGCATGACCTGTCGTCAGCGCTACGCTT
GCCCGATAACGGACGTGTCATGTTCAGCGGCCAGACTCCCGTCGGGCCGCTGCACATCCGCTATCGGCAGGGCGGCGAGG
TAATGGACCTGGCGGGTCGCGGTCATCGCGATCTCAAGCGCCTGCTCAATGAGCGCGCGGTGCCGGGCTTCGTGCGTGGC
AGATTGCCGCTGCTGTTTCGCGGTGAAGAATTGCTCGCCGTGGCGAACCTGCCGGGGCTCGACGGCAATGCGCTGGAAGG
CTGGCGATTGCATTGGCAGCCTTCGGACGAAGATCAAGGTTTGAGATGA

Upstream 100 bases:

>100_bases
TGCCCGCGAATGCGATGTAACATTCAACGACTGTGTTGGCTGTTTCAGCGCTTTCGCGAGCAAGCCCGCTCCCACATTTG
GTTTGTGAAAAGGTAGAAAA

Downstream 100 bases:

>100_bases
AGGGGGCATTCCGGTAGACTACGCTCCCTTCTTGATACAACTTCTGTGGATTCGCCTGAATTGCAGGAGTTGCCGATTAC
CAAGCAGTCTTTGCTGGGCG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MGQPSIDLPSRLLRNLKPWLGASHWRIAFSGGLDSTVLLHLLAKLAKNQSIPALSAIHVHHGLQAAADAWPAHCQAVCEE
LAVPLQVQRVQVQPGASVERAARDARYAAFSAATQANDVLLTGQHRDDQAETLLFRLLRGAGVRGLSGMPATRALGQGSL
VRPLLDVTRAELEAYARDHGLRWIEDPSNQDRQFSRNYLRHQIMPLLIGRWPQAHASMARSAAHLREAQGLLDELAQIDL
TQASLPSEFEWSGLPSLEFAAIAKLSDARQRNALSHWLEPLTRLPDTDHWSGWVDVRDAGNDASPIWRLADGELHRSAGR
LWWLSGEWLRTPVVSGDWHDLSSALRLPDNGRVMFSGQTPVGPLHIRYRQGGEVMDLAGRGHRDLKRLLNERAVPGFVRG
RLPLLFRGEELLAVANLPGLDGNALEGWRLHWQPSDEDQGLR

Sequences:

>Translated_442_residues
MGQPSIDLPSRLLRNLKPWLGASHWRIAFSGGLDSTVLLHLLAKLAKNQSIPALSAIHVHHGLQAAADAWPAHCQAVCEE
LAVPLQVQRVQVQPGASVERAARDARYAAFSAATQANDVLLTGQHRDDQAETLLFRLLRGAGVRGLSGMPATRALGQGSL
VRPLLDVTRAELEAYARDHGLRWIEDPSNQDRQFSRNYLRHQIMPLLIGRWPQAHASMARSAAHLREAQGLLDELAQIDL
TQASLPSEFEWSGLPSLEFAAIAKLSDARQRNALSHWLEPLTRLPDTDHWSGWVDVRDAGNDASPIWRLADGELHRSAGR
LWWLSGEWLRTPVVSGDWHDLSSALRLPDNGRVMFSGQTPVGPLHIRYRQGGEVMDLAGRGHRDLKRLLNERAVPGFVRG
RLPLLFRGEELLAVANLPGLDGNALEGWRLHWQPSDEDQGLR
>Mature_441_residues
GQPSIDLPSRLLRNLKPWLGASHWRIAFSGGLDSTVLLHLLAKLAKNQSIPALSAIHVHHGLQAAADAWPAHCQAVCEEL
AVPLQVQRVQVQPGASVERAARDARYAAFSAATQANDVLLTGQHRDDQAETLLFRLLRGAGVRGLSGMPATRALGQGSLV
RPLLDVTRAELEAYARDHGLRWIEDPSNQDRQFSRNYLRHQIMPLLIGRWPQAHASMARSAAHLREAQGLLDELAQIDLT
QASLPSEFEWSGLPSLEFAAIAKLSDARQRNALSHWLEPLTRLPDTDHWSGWVDVRDAGNDASPIWRLADGELHRSAGRL
WWLSGEWLRTPVVSGDWHDLSSALRLPDNGRVMFSGQTPVGPLHIRYRQGGEVMDLAGRGHRDLKRLLNERAVPGFVRGR
LPLLFRGEELLAVANLPGLDGNALEGWRLHWQPSDEDQGLR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=411, Percent_Identity=40.1459854014599, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PSEPF (Q3KH95)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346850.1
- ProteinModelPortal:   Q3KH95
- SMR:   Q3KH95
- STRING:   Q3KH95
- GeneID:   3716542
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1118
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   YRRGGEV
- ProtClustDB:   CLSK868125
- BioCyc:   PFLU205922:PFL_1118-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 49025; Mature: 48894

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQPSIDLPSRLLRNLKPWLGASHWRIAFSGGLDSTVLLHLLAKLAKNQSIPALSAIHVH
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
HGLQAAADAWPAHCQAVCEELAVPLQVQRVQVQPGASVERAARDARYAAFSAATQANDVL
HCHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
LTGQHRDDQAETLLFRLLRGAGVRGLSGMPATRALGQGSLVRPLLDVTRAELEAYARDHG
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LRWIEDPSNQDRQFSRNYLRHQIMPLLIGRWPQAHASMARSAAHLREAQGLLDELAQIDL
CEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQASLPSEFEWSGLPSLEFAAIAKLSDARQRNALSHWLEPLTRLPDTDHWSGWVDVRDAG
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
NDASPIWRLADGELHRSAGRLWWLSGEWLRTPVVSGDWHDLSSALRLPDNGRVMFSGQTP
CCCCCEEEECCCHHHHCCCCEEEECCCHHCCCCCCCCHHHHHHHHCCCCCCEEEEECCCC
VGPLHIRYRQGGEVMDLAGRGHRDLKRLLNERAVPGFVRGRLPLLFRGEELLAVANLPGL
CCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCEEEEEECCCCC
DGNALEGWRLHWQPSDEDQGLR
CCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
GQPSIDLPSRLLRNLKPWLGASHWRIAFSGGLDSTVLLHLLAKLAKNQSIPALSAIHVH
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
HGLQAAADAWPAHCQAVCEELAVPLQVQRVQVQPGASVERAARDARYAAFSAATQANDVL
HCHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
LTGQHRDDQAETLLFRLLRGAGVRGLSGMPATRALGQGSLVRPLLDVTRAELEAYARDHG
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LRWIEDPSNQDRQFSRNYLRHQIMPLLIGRWPQAHASMARSAAHLREAQGLLDELAQIDL
CEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQASLPSEFEWSGLPSLEFAAIAKLSDARQRNALSHWLEPLTRLPDTDHWSGWVDVRDAG
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
NDASPIWRLADGELHRSAGRLWWLSGEWLRTPVVSGDWHDLSSALRLPDNGRVMFSGQTP
CCCCCEEEECCCHHHHCCCCEEEECCCHHCCCCCCCCHHHHHHHHCCCCCCEEEEECCCC
VGPLHIRYRQGGEVMDLAGRGHRDLKRLLNERAVPGFVRGRLPLLFRGEELLAVANLPGL
CCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCEEEEEECCCCC
DGNALEGWRLHWQPSDEDQGLR
CCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA