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The map label for this gene is pcbC [H]

Identifier: 77456900

GI number: 77456900

Start: 786292

End: 787257

Strand: Reverse

Name: pcbC [H]

Synonym: Pfl01_0672

Alternate gene names: 77456900

Gene position: 787257-786292 (Counterclockwise)

Preceding gene: 77456901

Following gene: 77456899

Centisome position: 12.23

GC content: 63.04

Gene sequence:

>966_bases
ATGAACCAACTTCCGATCATCGACATCTCGCCGCTCTACTCCGACGACCAGAACGCCTGGCCGGCCGTCGCCGAACAGAT
CGACCACGCCTGCCGCGAATGGGGCTTCTTCTACATCAAGGGGCATCCGATTTCTGCGCAGCGCATTGCATCCCTGCTCG
ATCACGCGCAACGCTTCTTCGCGCTGCCGGAAGCAGAAAAGCTCAGGATCGACATCACCCAGACCCGTCATCACCGCGGC
TACGGGGCCATCGCCACCGAACAGCTCGACCCGGACAAACCCAGCGACCTCAAGGAAACCTTCGACATGGGCCTGCACCT
GCCGGCCGATCATCCCGAGGTGCTGGCAGTAAAACCCTTGCGCGGTCCCAACCGTCATCCGGCCATTCCCGGCTGGGAAA
CGTTGATGGAACAGCACTACCTCGACATGCAAGCGCTCGCGCAAACCCTGCTGCGTGCCATGACGTTAGCGCTGGGCATC
GAGCGCGACTTCTTCGACACGCGCTTCAACGAGCCGGTCAGCGTGCTGCGGATGATCCACTACCCGCCGCGCCACACCGC
CAGTTCCGCCGAACAGCAAGGTGCGGGCGCGCACACCGATTACGGCTGCATCACGCTGCTGTATCAGGACGCGGCCGGCG
GCTTGCAGGTGAAAAATGTCAAAGGCCAGTGGATCGACGCACCGCCCATCGACGGCACCTTCGTGGTCAACCTCGGCGAC
ATGATGGCGCGCTGGAGCAACGACCGTTATCGCTCGACCCCGCACCGGGTCATCAGCCCGCTCGGCGTGGATCGCTACTC
GATGCCATTCTTCGCCGAGCCGCATCCGGATACTCGCATCGAGTGCCTGCCCGGTTGCCAGGACGCACAGCATCCGGCGA
AATATCCGACCACCACCTGCGCCGAATTCCTGCTGTCGCGCTTCGCCGATACCTACGCCTATCGACGGGAGCAGGAAGCG
GTGTGA

Upstream 100 bases:

>100_bases
AACTACTACGTCGAAGGCATGAAGGCCGAGATGCCGAAGTAATCCACCTGCCCCTGACCGCTCCCTCTGCATCGGAGCGG
TCGCTAACGAGCATTCGAAA

Downstream 100 bases:

>100_bases
TGGATCGGTTGGTCAGGTTTTGCCAATTGTTTCGGCGGGCATCTGTAGAATGCTCGCCATTGCACCTGATGAGAAAAGAA
TATGTACGACTGGCTCAACG

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNQLPIIDISPLYSDDQNAWPAVAEQIDHACREWGFFYIKGHPISAQRIASLLDHAQRFFALPEAEKLRIDITQTRHHRG
YGAIATEQLDPDKPSDLKETFDMGLHLPADHPEVLAVKPLRGPNRHPAIPGWETLMEQHYLDMQALAQTLLRAMTLALGI
ERDFFDTRFNEPVSVLRMIHYPPRHTASSAEQQGAGAHTDYGCITLLYQDAAGGLQVKNVKGQWIDAPPIDGTFVVNLGD
MMARWSNDRYRSTPHRVISPLGVDRYSMPFFAEPHPDTRIECLPGCQDAQHPAKYPTTTCAEFLLSRFADTYAYRREQEA
V

Sequences:

>Translated_321_residues
MNQLPIIDISPLYSDDQNAWPAVAEQIDHACREWGFFYIKGHPISAQRIASLLDHAQRFFALPEAEKLRIDITQTRHHRG
YGAIATEQLDPDKPSDLKETFDMGLHLPADHPEVLAVKPLRGPNRHPAIPGWETLMEQHYLDMQALAQTLLRAMTLALGI
ERDFFDTRFNEPVSVLRMIHYPPRHTASSAEQQGAGAHTDYGCITLLYQDAAGGLQVKNVKGQWIDAPPIDGTFVVNLGD
MMARWSNDRYRSTPHRVISPLGVDRYSMPFFAEPHPDTRIECLPGCQDAQHPAKYPTTTCAEFLLSRFADTYAYRREQEA
V
>Mature_321_residues
MNQLPIIDISPLYSDDQNAWPAVAEQIDHACREWGFFYIKGHPISAQRIASLLDHAQRFFALPEAEKLRIDITQTRHHRG
YGAIATEQLDPDKPSDLKETFDMGLHLPADHPEVLAVKPLRGPNRHPAIPGWETLMEQHYLDMQALAQTLLRAMTLALGI
ERDFFDTRFNEPVSVLRMIHYPPRHTASSAEQQGAGAHTDYGCITLLYQDAAGGLQVKNVKGQWIDAPPIDGTFVVNLGD
MMARWSNDRYRSTPHRVISPLGVDRYSMPFFAEPHPDTRIECLPGCQDAQHPAKYPTTTCAEFLLSRFADTYAYRREQEA
V

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=340, Percent_Identity=25.8823529411765, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI85816264, Length=296, Percent_Identity=27.7027027027027, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 36342; Mature: 36342

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLPIIDISPLYSDDQNAWPAVAEQIDHACREWGFFYIKGHPISAQRIASLLDHAQRFF
CCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHH
ALPEAEKLRIDITQTRHHRGYGAIATEQLDPDKPSDLKETFDMGLHLPADHPEVLAVKPL
CCCCCCEEEEEEEHHHCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECC
RGPNRHPAIPGWETLMEQHYLDMQALAQTLLRAMTLALGIERDFFDTRFNEPVSVLRMIH
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHC
YPPRHTASSAEQQGAGAHTDYGCITLLYQDAAGGLQVKNVKGQWIDAPPIDGTFVVNLGD
CCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEEHHH
MMARWSNDRYRSTPHRVISPLGVDRYSMPFFAEPHPDTRIECLPGCQDAQHPAKYPTTTC
HHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHH
AEFLLSRFADTYAYRREQEAV
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQLPIIDISPLYSDDQNAWPAVAEQIDHACREWGFFYIKGHPISAQRIASLLDHAQRFF
CCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHH
ALPEAEKLRIDITQTRHHRGYGAIATEQLDPDKPSDLKETFDMGLHLPADHPEVLAVKPL
CCCCCCEEEEEEEHHHCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECC
RGPNRHPAIPGWETLMEQHYLDMQALAQTLLRAMTLALGIERDFFDTRFNEPVSVLRMIH
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHC
YPPRHTASSAEQQGAGAHTDYGCITLLYQDAAGGLQVKNVKGQWIDAPPIDGTFVVNLGD
CCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEEHHH
MMARWSNDRYRSTPHRVISPLGVDRYSMPFFAEPHPDTRIECLPGCQDAQHPAKYPTTTC
HHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHH
AEFLLSRFADTYAYRREQEAV
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1956290 [H]