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The map label for this gene is putP [H]

Identifier: 77456681

GI number: 77456681

Start: 519009

End: 520493

Strand: Direct

Name: putP [H]

Synonym: Pfl01_0453

Alternate gene names: 77456681

Gene position: 519009-520493 (Clockwise)

Preceding gene: 77456678

Following gene: 77456682

Centisome position: 8.06

GC content: 60.27

Gene sequence:

>1485_bases
ATGAGCGCAAGCAATCCAACCCTGATCACGTTCGTGATCTACATCGCAGCAATGGTGCTGATCGGCTTCATGGCCTATCG
CTCCACCAATAACCTTTCTGACTATATTCTGGGCGGCCGCAGCCTGGGCAGCGTGGTGACGGCATTGTCCGCCGGCGCTT
CCGACATGAGCGGCTGGTTGCTGATGGGCCTGCCGGGCGCCATCTACATGTCTGGCCTGTCCGAAAGCTGGATCGCCATC
GGCCTGATCGTCGGTGCCTACCTGAACTGGCTGTTCGTCGCCGGTCGTCTGCGTGTTCAGACCGAGCACAACGGCGATGC
ACTGACCCTGCCGGACTACTTCTCCAGCCGTTTCGAAGACAAGAGCGGTCTGCTGCGCATCATTTCGGCAGTGGTGATTC
TGGTGTTCTTCACCATCTACTGCGCTTCCGGCATCGTGGCCGGTGCCCGTCTGTTCGAAAGCACCTTCGGCATGTCCTAC
GAGACAGCGCTGTGGGCCGGTGCTGCGGCGACGATTGCCTACACCTTCGTCGGCGGTTTCCTCGCGGTGAGCTGGACCGA
CACCGTACAAGCCACCCTGATGATCTTCGCCCTGCTGCTGACGCCGATCATCGTGCTGCTGGCTACCGGCGGTGTCGACA
CCACGTTCCTGGCGATCGAAGCACAGGACCCGAGCAACTTCGACATGCTCAAGGGCACCACTTTCATCGGCATCATCTCG
CTGATGGGTTGGGGTCTGGGCTACTTCGGTCAGCCGCACATCCTGGCGCGCTTCATGGCCGCTGATTCGGTGAAATCCAT
CGCCAACGCCCGTCGCATCTCCATGACCTGGATGATCCTGTGCCTGGGCGGTACCGTGGCCGTGGGCTTCTTCGGTATCG
CTTACTTCTCGGCGCACCCGGAGGTGGCAGGTCCAGTGACCGAAAACCACGAGCGTGTGTTCATCGAACTGGCGAAAATC
CTGTTCAACCCGTGGATTGCAGGCGTGCTGTTGTCGGCTATTCTCGCGGCGGTGATGAGCACCCTGAGCTGCCAGTTGCT
GGTGTGCTCGAGCGCCCTGACCGAAGACTTCTACAAGGCTTTCCTGCGTAAATCCGCTTCGCAAGTCGAACTGGTATGGG
TCGGTCGCGCCATGGTGCTGCTGGTTGCACTGATCGCCATCGCGATGGCCGCCAACCCGGACAACCGTGTGCTGGGTCTG
GTGTCCTACGCCTGGGCCGGTTTCGGTGCAGCCTTCGGTCCGGTCGTGCTGATCTCGGTGATCTGGAAGAAAATGACCCG
TGACGGCGCACTGGCCGGCATCCTGGTCGGCGCGATCACCGTGATCGTGTGGAAGCATTTCGAACTGCTGGGTCTGTACG
AAATCATTCCGGGCTTCATCTTCGCCAGCCTGGCGATCTACTTCGTCAGCCTGATGGGCCAGCCGAGCGCCGGTATGGTT
CAGCGTTTTGAAGCGGCCGAGAAGGATTTCCATCTGAACAAGTGA

Upstream 100 bases:

>100_bases
GGTCATAAATGCGCGGCCGTGCGATTCGTCTGTCAGGCGTCAGTCTGCAAACGGTGCAACCGCCGCCGCTACATAAAAAC
AAAGCCAGGGCACGTACGTA

Downstream 100 bases:

>100_bases
TGGGAATGAGCTGAGACTCACCAGAAAACGGCCCGCGTCCTAAAGGAAGCGGGCCGTTTTTTATTGCCTGCGATTTTTCT
GAAGGAAAAGTCTGTAGTCG

Product: Sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSASNPTLITFVIYIAAMVLIGFMAYRSTNNLSDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIYMSGLSESWIAI
GLIVGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFEDKSGLLRIISAVVILVFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALLLTPIIVLLATGGVDTTFLAIEAQDPSNFDMLKGTTFIGIIS
LMGWGLGYFGQPHILARFMAADSVKSIANARRISMTWMILCLGGTVAVGFFGIAYFSAHPEVAGPVTENHERVFIELAKI
LFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAFLRKSASQVELVWVGRAMVLLVALIAIAMAANPDNRVLGL
VSYAWAGFGAAFGPVVLISVIWKKMTRDGALAGILVGAITVIVWKHFELLGLYEIIPGFIFASLAIYFVSLMGQPSAGMV
QRFEAAEKDFHLNK

Sequences:

>Translated_494_residues
MSASNPTLITFVIYIAAMVLIGFMAYRSTNNLSDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIYMSGLSESWIAI
GLIVGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFEDKSGLLRIISAVVILVFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALLLTPIIVLLATGGVDTTFLAIEAQDPSNFDMLKGTTFIGIIS
LMGWGLGYFGQPHILARFMAADSVKSIANARRISMTWMILCLGGTVAVGFFGIAYFSAHPEVAGPVTENHERVFIELAKI
LFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAFLRKSASQVELVWVGRAMVLLVALIAIAMAANPDNRVLGL
VSYAWAGFGAAFGPVVLISVIWKKMTRDGALAGILVGAITVIVWKHFELLGLYEIIPGFIFASLAIYFVSLMGQPSAGMV
QRFEAAEKDFHLNK
>Mature_493_residues
SASNPTLITFVIYIAAMVLIGFMAYRSTNNLSDYILGGRSLGSVVTALSAGASDMSGWLLMGLPGAIYMSGLSESWIAIG
LIVGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFEDKSGLLRIISAVVILVFFTIYCASGIVAGARLFESTFGMSYE
TALWAGAAATIAYTFVGGFLAVSWTDTVQATLMIFALLLTPIIVLLATGGVDTTFLAIEAQDPSNFDMLKGTTFIGIISL
MGWGLGYFGQPHILARFMAADSVKSIANARRISMTWMILCLGGTVAVGFFGIAYFSAHPEVAGPVTENHERVFIELAKIL
FNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAFLRKSASQVELVWVGRAMVLLVALIAIAMAANPDNRVLGLV
SYAWAGFGAAFGPVVLISVIWKKMTRDGALAGILVGAITVIVWKHFELLGLYEIIPGFIFASLAIYFVSLMGQPSAGMVQ
RFEAAEKDFHLNK

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=503, Percent_Identity=46.7196819085487, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI110835708, Length=485, Percent_Identity=24.9484536082474, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1787251, Length=492, Percent_Identity=75.8130081300813, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1790503, Length=450, Percent_Identity=27.1111111111111, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87082237, Length=445, Percent_Identity=26.9662921348315, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790113, Length=462, Percent_Identity=23.5930735930736, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533094, Length=377, Percent_Identity=24.9336870026525, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221459584, Length=392, Percent_Identity=24.234693877551, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24645928, Length=384, Percent_Identity=25.5208333333333, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI221459588, Length=379, Percent_Identity=24.802110817942, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24651741, Length=459, Percent_Identity=22.8758169934641, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640370, Length=355, Percent_Identity=22.5352112676056, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221459586, Length=381, Percent_Identity=22.5721784776903, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221459582, Length=375, Percent_Identity=25.0666666666667, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53030; Mature: 52899

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASNPTLITFVIYIAAMVLIGFMAYRSTNNLSDYILGGRSLGSVVTALSAGASDMSGWL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCEE
LMGLPGAIYMSGLSESWIAIGLIVGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFED
EECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEECCHHHHHHCCC
KSGLLRIISAVVILVFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATIAYTFVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQATLMIFALLLTPIIVLLATGGVDTTFLAIEAQDPSNFDMLKGTTFIGIIS
HEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCHHHHHHHH
LMGWGLGYFGQPHILARFMAADSVKSIANARRISMTWMILCLGGTVAVGFFGIAYFSAHP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVAGPVTENHERVFIELAKILFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKSASQVELVWVGRAMVLLVALIAIAMAANPDNRVLGLVSYAWAGFGAAFGPVVLISV
HHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCHHHHHHHHHHH
IWKKMTRDGALAGILVGAITVIVWKHFELLGLYEIIPGFIFASLAIYFVSLMGQPSAGMV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QRFEAAEKDFHLNK
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SASNPTLITFVIYIAAMVLIGFMAYRSTNNLSDYILGGRSLGSVVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCEE
LMGLPGAIYMSGLSESWIAIGLIVGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFED
EECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEECCHHHHHHCCC
KSGLLRIISAVVILVFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATIAYTFVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQATLMIFALLLTPIIVLLATGGVDTTFLAIEAQDPSNFDMLKGTTFIGIIS
HEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCHHHHHHHH
LMGWGLGYFGQPHILARFMAADSVKSIANARRISMTWMILCLGGTVAVGFFGIAYFSAHP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVAGPVTENHERVFIELAKILFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKSASQVELVWVGRAMVLLVALIAIAMAANPDNRVLGLVSYAWAGFGAAFGPVVLISV
HHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCHHHHHHHHHHH
IWKKMTRDGALAGILVGAITVIVWKHFELLGLYEIIPGFIFASLAIYFVSLMGQPSAGMV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QRFEAAEKDFHLNK
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]