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The map label for this gene is pyrE [H]

Identifier: 75674352

GI number: 75674352

Start: 178339

End: 178902

Strand: Direct

Name: pyrE [H]

Synonym: Nwi_0153

Alternate gene names: 75674352

Gene position: 178339-178902 (Clockwise)

Preceding gene: 75674351

Following gene: 75674355

Centisome position: 5.24

GC content: 57.27

Gene sequence:

>564_bases
ATGCCTGAAGATATAAAAGTCCGTCAAAGGGCTTTCGAGATCATCAAGACCAGATCATTCGGCCGCGGCAACATCAAACT
GGCCTCCGGAAAAACCAGCACGTTCTACTTCGATATGAAACCGACGATGCTGGCTCCTGAAGGGGCCCATGTCCTGTCCG
AGCTCATTCTGGCGCGGCTTGCCGGCCAAGGCATCGACTATATCGGCGGGCTGGAGATGGGAGCGGTGCCTCTGATCTCG
ACCGTCAGCCTTCTCAGCCACATCAGGAATGAACCGCTTCCAGGCTTCTTCGTTCGCAAGGACGTCAAGAAGCACGGAAC
CCGGAAACTCATCGAGGGTCTCCCCGTCGATGATCTTCGCGGCAAGAGGGTCGTCATCCTGGAGGATGTCACGACGAGCG
GCGCGTCCGCCATGATCGCGGTCTCGGCCGCGCAGGCCGAGGGCGCGGAAGTCGTGATGGTTCTGTCGATCGTCGACAGG
GGCGAGGGAGCCATGGCTCTCTATAAAGAGAAAAGCATTGAATTCGATTCGCTGTTCAAGGCGGAAGAATTCCTGGCTTC
CTAA

Upstream 100 bases:

>100_bases
TCGTCTGGTTCGTTGTCACGACGACCGGTTGAGCGTATAGACCGGCAATCCTCCGTTCCGGCACGACAGTTTCAATTCAC
ATCGACCGAGCCCGAAAGCC

Downstream 100 bases:

>100_bases
CGCTCCTTCTGAAAGGCCGTCCGTCCGATAATCACCGGCTTGCGCCGTTGCACCAGCTTTCGCGACGGCCGCCATGATAG
ACGCGGGCGAGGCCGCTCGC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MPEDIKVRQRAFEIIKTRSFGRGNIKLASGKTSTFYFDMKPTMLAPEGAHVLSELILARLAGQGIDYIGGLEMGAVPLIS
TVSLLSHIRNEPLPGFFVRKDVKKHGTRKLIEGLPVDDLRGKRVVILEDVTTSGASAMIAVSAAQAEGAEVVMVLSIVDR
GEGAMALYKEKSIEFDSLFKAEEFLAS

Sequences:

>Translated_187_residues
MPEDIKVRQRAFEIIKTRSFGRGNIKLASGKTSTFYFDMKPTMLAPEGAHVLSELILARLAGQGIDYIGGLEMGAVPLIS
TVSLLSHIRNEPLPGFFVRKDVKKHGTRKLIEGLPVDDLRGKRVVILEDVTTSGASAMIAVSAAQAEGAEVVMVLSIVDR
GEGAMALYKEKSIEFDSLFKAEEFLAS
>Mature_186_residues
PEDIKVRQRAFEIIKTRSFGRGNIKLASGKTSTFYFDMKPTMLAPEGAHVLSELILARLAGQGIDYIGGLEMGAVPLIST
VSLLSHIRNEPLPGFFVRKDVKKHGTRKLIEGLPVDDLRGKRVVILEDVTTSGASAMIAVSAAQAEGAEVVMVLSIVDRG
EGAMALYKEKSIEFDSLFKAEEFLAS

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=172, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508631, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=178, Percent_Identity=29.2134831460674, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17933654, Length=176, Percent_Identity=29.5454545454545, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 20310; Mature: 20179

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEDIKVRQRAFEIIKTRSFGRGNIKLASGKTSTFYFDMKPTMLAPEGAHVLSELILARL
CCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEECCCHHHHHHHHHHHHH
AGQGIDYIGGLEMGAVPLISTVSLLSHIRNEPLPGFFVRKDVKKHGTRKLIEGLPVDDLR
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GKRVVILEDVTTSGASAMIAVSAAQAEGAEVVMVLSIVDRGEGAMALYKEKSIEFDSLFK
CCEEEEEECCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCEEEECCCCCCHHHHHH
AEEFLAS
HHHHHCC
>Mature Secondary Structure 
PEDIKVRQRAFEIIKTRSFGRGNIKLASGKTSTFYFDMKPTMLAPEGAHVLSELILARL
CCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEECCCHHHHHHHHHHHHH
AGQGIDYIGGLEMGAVPLISTVSLLSHIRNEPLPGFFVRKDVKKHGTRKLIEGLPVDDLR
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GKRVVILEDVTTSGASAMIAVSAAQAEGAEVVMVLSIVDRGEGAMALYKEKSIEFDSLFK
CCEEEEEECCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCEEEECCCCCCHHHHHH
AEEFLAS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]