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The map label for this gene is rsmB [H]

Identifier: 75674355

GI number: 75674355

Start: 180146

End: 181498

Strand: Direct

Name: rsmB [H]

Synonym: Nwi_0156

Alternate gene names: 75674355

Gene position: 180146-181498 (Clockwise)

Preceding gene: 75674352

Following gene: 75674356

Centisome position: 5.3

GC content: 68.44

Gene sequence:

>1353_bases
ATGCCGTCCACCAGATTCGCGCCGCCGACCGAAGCTCCGGGCCTCGCGGCCCGGCGCATCGCAGCGGATATCCTCGACGG
TGTCCTGCATAAGCGACGCACCCTCGACGAACAACTCGAAGGCCCGACGGCGCATCCCGGCCTGAAAGCGCTTGCCGACC
GCGACCGCGCCTTGATGCGACGCCTGGTGGCGACGATCCTGCGCCGGCTCGGCACCCTCGGTCATTTGCTGTCGCGGCTG
CTCGATCGCGGCATTCCGACCGATGCGCCGCGCGCGCAGAGCGCGCTTCTGATCGGGGCGGCGCAGATTCTGTGGATGGA
TGTGCCGGATCATGCCGCGGTCGATCTGTCGGTGCGGCTCGTGCAATCCGATCGCCGCGCGGCGAAATATGCCGGATTGG
TCAACGCGGTGCTGCGCCGATGCGCGCGCGAAGGCCAGTCCCTGATCGAGGAAGTCCGCTCGGAATCGCTCGATACGCCG
TCATGGCTGATGGCGCGCTGGAGCGGCTACTACGGCGAGGGCACCGCGAAAGCGATTGCCGCCGCGATCAGCCATGAGCC
TGCCCTCGATCTCACCGTCAAAGCGAACGCCGCTCACTGGGCGACGCGGCTGCACGGCGAAACCTTGCCGACCGGAACGG
TTCGCACCTCGTTGCACGGCTCGGTGACGATGCTGCCGGGATTCTCCGAGGGGCAGTGGTGGGTCCAGGACGCCGCCGCC
GCGCTGCCCGCGCGCCTGTTCGGCGATCTCAGAGGACGGCGGGTCATCGACCTCTGCGCGGCGCCCGGCGGCAAGACCGC
CCAACTCGTACAGGCCGGCGCGGAGGTGACCGCGATCGACCGGTCGCCGAACCGGGTGGCGCGGCTGCGCGAAAATCTTG
GCCGTCTGTCGCTGGACGCGCGATCGCTCGTGGCCGACGCCATCGAATGGCAGGATGATGTCGCGGGCGACGGTTTCGAC
GGCGTGCTCGTCGACGCGCCTTGTTCCGCGACCGGCACCATCCGCCGTCATCCGGACGTGGCCTGGCTCAAGCAGGATTC
CGATATCGCGGCGCTGACCGCCTTGCAGCAAAGGCTCCTGAACAAGGCGATCACGCTGCTCAGGCCCGGCGGAACGCTGG
TCTACTGCACCTGTTCGCTGGAACCCGAGGAGGGTGAGCAGGTCGTTGCCGCGCTGCTTGCGACCGACACGGGGATCCGG
CGCGTGCCGGTCAGCGCGGACGAAGTGGCGGGGCTCGCGGATATCGTGACGCCCGAGGGCGATCTGCGTACGTTGCCCTG
CCATCTCGCGCATGATGATCCCACGCTCGGCGGATTAGACGGCTTCTACGCGGCGCGATTGACCAGGTCCTGA

Upstream 100 bases:

>100_bases
ATATTTGGCATATTCGCGTGTCACGAATTCGGACTGCCGCGGCGGGCGCACATCAAATGCGTCCACCGTCCCTGATTCCC
TTAAGCGATGTGCCTGCCTC

Downstream 100 bases:

>100_bases
TTCGCAAGGCGGAAGCGGAGATTCGCGGCATTTCAAGATGGTGCGTTGCCGGCCTTTCCGGATTAAAAGGATTCAGTCCT
CATCTCCGCAAAGGGATTCG

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPSTRFAPPTEAPGLAARRIAADILDGVLHKRRTLDEQLEGPTAHPGLKALADRDRALMRRLVATILRRLGTLGHLLSRL
LDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGQSLIEEVRSESLDTP
SWLMARWSGYYGEGTAKAIAAAISHEPALDLTVKANAAHWATRLHGETLPTGTVRTSLHGSVTMLPGFSEGQWWVQDAAA
ALPARLFGDLRGRRVIDLCAAPGGKTAQLVQAGAEVTAIDRSPNRVARLRENLGRLSLDARSLVADAIEWQDDVAGDGFD
GVLVDAPCSATGTIRRHPDVAWLKQDSDIAALTALQQRLLNKAITLLRPGGTLVYCTCSLEPEEGEQVVAALLATDTGIR
RVPVSADEVAGLADIVTPEGDLRTLPCHLAHDDPTLGGLDGFYAARLTRS

Sequences:

>Translated_450_residues
MPSTRFAPPTEAPGLAARRIAADILDGVLHKRRTLDEQLEGPTAHPGLKALADRDRALMRRLVATILRRLGTLGHLLSRL
LDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGQSLIEEVRSESLDTP
SWLMARWSGYYGEGTAKAIAAAISHEPALDLTVKANAAHWATRLHGETLPTGTVRTSLHGSVTMLPGFSEGQWWVQDAAA
ALPARLFGDLRGRRVIDLCAAPGGKTAQLVQAGAEVTAIDRSPNRVARLRENLGRLSLDARSLVADAIEWQDDVAGDGFD
GVLVDAPCSATGTIRRHPDVAWLKQDSDIAALTALQQRLLNKAITLLRPGGTLVYCTCSLEPEEGEQVVAALLATDTGIR
RVPVSADEVAGLADIVTPEGDLRTLPCHLAHDDPTLGGLDGFYAARLTRS
>Mature_449_residues
PSTRFAPPTEAPGLAARRIAADILDGVLHKRRTLDEQLEGPTAHPGLKALADRDRALMRRLVATILRRLGTLGHLLSRLL
DRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGQSLIEEVRSESLDTPS
WLMARWSGYYGEGTAKAIAAAISHEPALDLTVKANAAHWATRLHGETLPTGTVRTSLHGSVTMLPGFSEGQWWVQDAAAA
LPARLFGDLRGRRVIDLCAAPGGKTAQLVQAGAEVTAIDRSPNRVARLRENLGRLSLDARSLVADAIEWQDDVAGDGFDG
VLVDAPCSATGTIRRHPDVAWLKQDSDIAALTALQQRLLNKAITLLRPGGTLVYCTCSLEPEEGEQVVAALLATDTGIRR
VPVSADEVAGLADIVTPEGDLRTLPCHLAHDDPTLGGLDGFYAARLTRS

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=189, Percent_Identity=36.5079365079365, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI76150623, Length=189, Percent_Identity=36.5079365079365, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI32698918, Length=172, Percent_Identity=33.7209302325581, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI40316918, Length=187, Percent_Identity=35.8288770053476, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI301336155, Length=196, Percent_Identity=33.6734693877551, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI39995082, Length=196, Percent_Identity=33.6734693877551, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI11545785, Length=183, Percent_Identity=34.4262295081967, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI270288816, Length=186, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI23199998, Length=186, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI270288818, Length=186, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI8922322, Length=186, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI2367212, Length=449, Percent_Identity=31.8485523385301, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI87081985, Length=258, Percent_Identity=32.1705426356589, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=233, Percent_Identity=30.9012875536481, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI115534021, Length=230, Percent_Identity=33.0434782608696, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71998419, Length=230, Percent_Identity=33.0434782608696, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=179, Percent_Identity=35.195530726257, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=238, Percent_Identity=29.8319327731092, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319447, Length=177, Percent_Identity=34.4632768361582, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI22024126, Length=237, Percent_Identity=29.957805907173, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24668781, Length=205, Percent_Identity=30.2439024390244, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356513, Length=205, Percent_Identity=30.2439024390244, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355201, Length=184, Percent_Identity=32.6086956521739, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920494, Length=186, Percent_Identity=31.1827956989247, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356579, Length=179, Percent_Identity=28.4916201117318, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48228; Mature: 48096

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTRFAPPTEAPGLAARRIAADILDGVLHKRRTLDEQLEGPTAHPGLKALADRDRALMR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RLVATILRRLGTLGHLLSRLLDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEEEEEEE
VQSDRRAAKYAGLVNAVLRRCAREGQSLIEEVRSESLDTPSWLMARWSGYYGEGTAKAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
AAISHEPALDLTVKANAAHWATRLHGETLPTGTVRTSLHGSVTMLPGFSEGQWWVQDAAA
HHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHH
ALPARLFGDLRGRRVIDLCAAPGGKTAQLVQAGAEVTAIDRSPNRVARLRENLGRLSLDA
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCHH
RSLVADAIEWQDDVAGDGFDGVLVDAPCSATGTIRRHPDVAWLKQDSDIAALTALQQRLL
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
NKAITLLRPGGTLVYCTCSLEPEEGEQVVAALLATDTGIRRVPVSADEVAGLADIVTPEG
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEECCCCHHHHCCHHHHCCCCC
DLRTLPCHLAHDDPTLGGLDGFYAARLTRS
CCEECCEEECCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
PSTRFAPPTEAPGLAARRIAADILDGVLHKRRTLDEQLEGPTAHPGLKALADRDRALMR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RLVATILRRLGTLGHLLSRLLDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEEEEEEE
VQSDRRAAKYAGLVNAVLRRCAREGQSLIEEVRSESLDTPSWLMARWSGYYGEGTAKAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
AAISHEPALDLTVKANAAHWATRLHGETLPTGTVRTSLHGSVTMLPGFSEGQWWVQDAAA
HHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHH
ALPARLFGDLRGRRVIDLCAAPGGKTAQLVQAGAEVTAIDRSPNRVARLRENLGRLSLDA
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCHH
RSLVADAIEWQDDVAGDGFDGVLVDAPCSATGTIRRHPDVAWLKQDSDIAALTALQQRLL
HHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
NKAITLLRPGGTLVYCTCSLEPEEGEQVVAALLATDTGIRRVPVSADEVAGLADIVTPEG
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEECCCCHHHHCCHHHHCCCCC
DLRTLPCHLAHDDPTLGGLDGFYAARLTRS
CCEECCEEECCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA