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The map label for this gene is lysK [H]

Identifier: 75674350

GI number: 75674350

Start: 175870

End: 177501

Strand: Direct

Name: lysK [H]

Synonym: Nwi_0151

Alternate gene names: 75674350

Gene position: 175870-177501 (Clockwise)

Preceding gene: 75674349

Following gene: 75674351

Centisome position: 5.17

GC content: 63.05

Gene sequence:

>1632_bases
ATGTCAGACCTCATGTCCGCGGGCGATCTGCGTGCGCTCGCCGAGCAATCCAATGCGTGGCCGTTCGAGCAGGCGAAATT
GATCGTCGCGCGTCTGAAGAAAACGCCGAAGGACGAGGTGCTGTTCGAGACCGGCTACGGCCCCTCCGGACTGCCGCATA
TCGGCACCTTCGGCGAGGTCGCGCGCACCACCATGGTGCGTCATGCGTTCCGGGTGCTGACGGAGGACAAGATCAAGACG
CGGCTGCTCGCATTCTCCGACGACATGGACGGCCTGCGCAAGGTGCCGGACAATGTGCCCAACAAGGAGCTGCTGGCGCG
GGATCTCGGCAAGCCGCTGACGAAGGTAGTGGATCCCTTCGGCACCCATTCGAGCTTCGGCGAGCATAACAACGCCCGGC
TGCGCGCTTTCCTCGACACTTTCGGTTTCGATTACGAATTCGCCAGTTCGACCGCCTATTACACATCTGGTCGCTTCGAC
GTGACGCTTCTGAAGGTGCTTGAGAACATAGACAAGGTGATGGCGATCATGCTGCCGTCCCTGCGTGAGGAGCGCGCGGC
GAGCTATTCGCCGTTCCTGCCCATCTGCCCGCGCACCGGCGTGGTGTTGCAGGTGCCGATATCCGCGCACGACGCCAAGG
CCGGCACGGTTTCCTACGACGACCCAGAGACTAAAGAGCGCGTCACCGTGCCCGTCACCGGCGGGCGCTGCAAACTCCAA
TGGAAGCCCGACTGGGCGATGCGCTGGACCGCGCTCGGCGTCGATTACGAGATGGCCGGCAAGGACTTGATCGATTCCGT
CAAGCTGTCCGGCAAAATCTGTTCGGCGCTCGGCGGCACGCCGCCGGAAGGCTTCAACTACGAACTGTTCCTCGACGACA
AGGGCCAGAAGATTTCCAAGTCGAAGGGCAACGGCCTCACCATCGACGAGTGGCTGCGCTATGCCTCGCCGGAATCATTG
TCGCTGTTCATGTACCGCGAACCGAAGGCGGCGAAGCGGCTGTATTTCGACGTGATTCCGCGCAACGTCGACGACTACCA
GCAGTTCCTCGAAGGCTATCCGCGCCAGGATGCGAGGCAGCAGCTCGCCAATCCGGTCTGGCACATTCATGCCGGCCATC
CGCCCTCGGCCGACATGCCGGTCACGTTCCAGTTGCTGCTGACGCTGGTCTCGTCCTCGAACGCGGAGAATGCCGAAACT
CTGTGGGGCTTCATCGGCCGCTACCGGCCGGGCGTCACGCCGCAGACCCATCCGAAGCTCGATGCGCTGGTCGGCTATGC
CATCAACTATTACCGTGACTTCGTGGCGCCGACGAAAACATTCCGCGAGCCGACCGCGAGCGAGCGCGCCGCCTTGCAGG
ACCTGCGCGAAGCGCTGTCGCAACTGCCCGCGGACGCCTCGGCCGAAGACATCCAGAACACGGTCTACGAGATCGGCCGC
CGCGAACCTTTCCTCAATCACAAGAAGCCCGCCAGGGATGGTCGTCCCGGCGTCTCGCTCGACTGGTTCAACATGCTCTA
TCAGGTGCTGCTCGGTCAGGAGAAGGGACCGCGCTTCGGCTCCTTCGTCGCGGTCTACGGCGTGCAGAACGCCATCGCGA
TGATCGACGGCGCATTGGCGCGCGCGGATTAG

Upstream 100 bases:

>100_bases
GTGATTCCGAAAAGTGGGAACCGGTTTTCGGATAAGATCATGCTCAATCAAAGAGATACGGGTGGCGCGCCGGACGATGC
CGCACGCCCTGGAGAGTATA

Downstream 100 bases:

>100_bases
AGCATGATCCCGAAAAGTGGAAACCGGTTTTCGGATGAGATCATGCTCGATAAAGAAAGTGAACGCTGGAGTCCCTCTCC
ATCGGGTCATTTGATGCGAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSDLMSAGDLRALAEQSNAWPFEQAKLIVARLKKTPKDEVLFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDKIKT
RLLAFSDDMDGLRKVPDNVPNKELLARDLGKPLTKVVDPFGTHSSFGEHNNARLRAFLDTFGFDYEFASSTAYYTSGRFD
VTLLKVLENIDKVMAIMLPSLREERAASYSPFLPICPRTGVVLQVPISAHDAKAGTVSYDDPETKERVTVPVTGGRCKLQ
WKPDWAMRWTALGVDYEMAGKDLIDSVKLSGKICSALGGTPPEGFNYELFLDDKGQKISKSKGNGLTIDEWLRYASPESL
SLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGYPRQDARQQLANPVWHIHAGHPPSADMPVTFQLLLTLVSSSNAENAET
LWGFIGRYRPGVTPQTHPKLDALVGYAINYYRDFVAPTKTFREPTASERAALQDLREALSQLPADASAEDIQNTVYEIGR
REPFLNHKKPARDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGVQNAIAMIDGALARAD

Sequences:

>Translated_543_residues
MSDLMSAGDLRALAEQSNAWPFEQAKLIVARLKKTPKDEVLFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDKIKT
RLLAFSDDMDGLRKVPDNVPNKELLARDLGKPLTKVVDPFGTHSSFGEHNNARLRAFLDTFGFDYEFASSTAYYTSGRFD
VTLLKVLENIDKVMAIMLPSLREERAASYSPFLPICPRTGVVLQVPISAHDAKAGTVSYDDPETKERVTVPVTGGRCKLQ
WKPDWAMRWTALGVDYEMAGKDLIDSVKLSGKICSALGGTPPEGFNYELFLDDKGQKISKSKGNGLTIDEWLRYASPESL
SLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGYPRQDARQQLANPVWHIHAGHPPSADMPVTFQLLLTLVSSSNAENAET
LWGFIGRYRPGVTPQTHPKLDALVGYAINYYRDFVAPTKTFREPTASERAALQDLREALSQLPADASAEDIQNTVYEIGR
REPFLNHKKPARDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGVQNAIAMIDGALARAD
>Mature_542_residues
SDLMSAGDLRALAEQSNAWPFEQAKLIVARLKKTPKDEVLFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDKIKTR
LLAFSDDMDGLRKVPDNVPNKELLARDLGKPLTKVVDPFGTHSSFGEHNNARLRAFLDTFGFDYEFASSTAYYTSGRFDV
TLLKVLENIDKVMAIMLPSLREERAASYSPFLPICPRTGVVLQVPISAHDAKAGTVSYDDPETKERVTVPVTGGRCKLQW
KPDWAMRWTALGVDYEMAGKDLIDSVKLSGKICSALGGTPPEGFNYELFLDDKGQKISKSKGNGLTIDEWLRYASPESLS
LFMYREPKAAKRLYFDVIPRNVDDYQQFLEGYPRQDARQQLANPVWHIHAGHPPSADMPVTFQLLLTLVSSSNAENAETL
WGFIGRYRPGVTPQTHPKLDALVGYAINYYRDFVAPTKTFREPTASERAALQDLREALSQLPADASAEDIQNTVYEIGRR
EPFLNHKKPARDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGVQNAIAMIDGALARAD

Specific function: Unknown

COG id: COG1384

COG function: function code J; Lysyl-tRNA synthetase (class I)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR002904
- InterPro:   IPR020754
- InterPro:   IPR020756
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01921 tRNA-synt_1f [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 60597; Mature: 60465

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLMSAGDLRALAEQSNAWPFEQAKLIVARLKKTPKDEVLFETGYGPSGLPHIGTFGEV
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCCHHHH
ARTTMVRHAFRVLTEDKIKTRLLAFSDDMDGLRKVPDNVPNKELLARDLGKPLTKVVDPF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHCCHHHHHHCCC
GTHSSFGEHNNARLRAFLDTFGFDYEFASSTAYYTSGRFDVTLLKVLENIDKVMAIMLPS
CCCCCCCCCCCHHHHHHHHHHCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LREERAASYSPFLPICPRTGVVLQVPISAHDAKAGTVSYDDPETKERVTVPVTGGRCKLQ
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEE
WKPDWAMRWTALGVDYEMAGKDLIDSVKLSGKICSALGGTPPEGFNYELFLDDKGQKISK
ECCCHHEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHC
SKGNGLTIDEWLRYASPESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGYPRQDARQ
CCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
QLANPVWHIHAGHPPSADMPVTFQLLLTLVSSSNAENAETLWGFIGRYRPGVTPQTHPKL
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
DALVGYAINYYRDFVAPTKTFREPTASERAALQDLREALSQLPADASAEDIQNTVYEIGR
HHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
REPFLNHKKPARDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGVQNAIAMIDGALA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
RAD
CCC
>Mature Secondary Structure 
SDLMSAGDLRALAEQSNAWPFEQAKLIVARLKKTPKDEVLFETGYGPSGLPHIGTFGEV
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCCHHHH
ARTTMVRHAFRVLTEDKIKTRLLAFSDDMDGLRKVPDNVPNKELLARDLGKPLTKVVDPF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHCCHHHHHHCCC
GTHSSFGEHNNARLRAFLDTFGFDYEFASSTAYYTSGRFDVTLLKVLENIDKVMAIMLPS
CCCCCCCCCCCHHHHHHHHHHCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LREERAASYSPFLPICPRTGVVLQVPISAHDAKAGTVSYDDPETKERVTVPVTGGRCKLQ
HHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEE
WKPDWAMRWTALGVDYEMAGKDLIDSVKLSGKICSALGGTPPEGFNYELFLDDKGQKISK
ECCCHHEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHC
SKGNGLTIDEWLRYASPESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGYPRQDARQ
CCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
QLANPVWHIHAGHPPSADMPVTFQLLLTLVSSSNAENAETLWGFIGRYRPGVTPQTHPKL
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
DALVGYAINYYRDFVAPTKTFREPTASERAALQDLREALSQLPADASAEDIQNTVYEIGR
HHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
REPFLNHKKPARDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGVQNAIAMIDGALA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
RAD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]