Click here to switch to the map view.

The map label for this gene is artI [H]

Identifier: 75674349

GI number: 75674349

Start: 174846

End: 175766

Strand: Direct

Name: artI [H]

Synonym: Nwi_0150

Alternate gene names: 75674349

Gene position: 174846-175766 (Clockwise)

Preceding gene: 75674345

Following gene: 75674350

Centisome position: 5.14

GC content: 63.52

Gene sequence:

>921_bases
ATGCAACCACAGATTACCGTTTCCGCCAACGAGAGATTGATCCGCCGCGGCGCCGCAACGCTGGGGTGTCTGCTGGCCGC
CGGGTGGATGCTGGGCGCCTGGGCCACGCTCGACCGCGCGCGTGCCCAGACCACGGTGGAAACCGCGACCGCCGCGCCGC
AGGCCGTGCCGGGCTTCTGGGACCCGCGCCACCGTCCCGACCGTCCCGATCTATCCCGCCTCACCGTGATTCGGTTTTTG
ACGGAAACCGACTATCCACCCTTCAACTATACCGGTCCCGACGGCAATCCGGCCGGCTTCAATGTCGATCTGGCGCGCAC
CTTGTGCGAGGAGATCAAGGTCACCTGCACGATTCAGATGCGGCGGTTCGAGACGCTGGTGGACGCGCTGTCGAGTAACC
GCGGCGACGCCCTCATCGCCTCCCTCGCCGTCACTCCCGAGTTGCGCAAGCGTATCGATTTCACCGATCCCTATTATCGG
ACGCCGGCCCGGTTCGTATCACGGCGCGACGCCGCCATGGCCGAGGTGCGCCCGGAATATCTGGAGGGGAAGAAGGTCGG
CGTCGTCGCGGGGTCCGCGCACGAGGCCTATCTCAAGGTTTTCTTCACCGACGCCGAACTGCACGGCTACCCGAACGACG
AGACGCTGCGGCAGGCGCTGCGGCAGAACGAGGTGGACTTCATTTTCGGTGACGCGATTTCACTGGCGTTCTGGATCAAC
GGCACGGATTCTGAAAGCTGCTGCGCGTTCAGCGGCGGCCCCTTTATCGAAAGCCGCTATTTCGGCGAAGGCATCGGCAT
TGCCGTGAGAAAAGGCAATGACGTGCTGCGGCAGGCGTTGAACTGGGCGCTCTTCCGAATCTGGGAGAGAGGCCGCTATA
CCGATCTGTGGCTGCGGTATTTTTCCGTCAGCCCGTTTTAA

Upstream 100 bases:

>100_bases
CCCACGCGATTCCGCCCCGCCAAGCCACGTCCCCACGCAAACGATACTCGCGTCCCGTTCCGGATGGTCTATAAACCCTC
CGTTCGAAAAGCCCCTGTCC

Downstream 100 bases:

>100_bases
AGCGTGATTCCGAAAAGTGGGAACCGGTTTTCGGATAAGATCATGCTCAATCAAAGAGATACGGGTGGCGCGCCGGACGA
TGCCGCACGCCCTGGAGAGT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQPQITVSANERLIRRGAATLGCLLAAGWMLGAWATLDRARAQTTVETATAAPQAVPGFWDPRHRPDRPDLSRLTVIRFL
TETDYPPFNYTGPDGNPAGFNVDLARTLCEEIKVTCTIQMRRFETLVDALSSNRGDALIASLAVTPELRKRIDFTDPYYR
TPARFVSRRDAAMAEVRPEYLEGKKVGVVAGSAHEAYLKVFFTDAELHGYPNDETLRQALRQNEVDFIFGDAISLAFWIN
GTDSESCCAFSGGPFIESRYFGEGIGIAVRKGNDVLRQALNWALFRIWERGRYTDLWLRYFSVSPF

Sequences:

>Translated_306_residues
MQPQITVSANERLIRRGAATLGCLLAAGWMLGAWATLDRARAQTTVETATAAPQAVPGFWDPRHRPDRPDLSRLTVIRFL
TETDYPPFNYTGPDGNPAGFNVDLARTLCEEIKVTCTIQMRRFETLVDALSSNRGDALIASLAVTPELRKRIDFTDPYYR
TPARFVSRRDAAMAEVRPEYLEGKKVGVVAGSAHEAYLKVFFTDAELHGYPNDETLRQALRQNEVDFIFGDAISLAFWIN
GTDSESCCAFSGGPFIESRYFGEGIGIAVRKGNDVLRQALNWALFRIWERGRYTDLWLRYFSVSPF
>Mature_306_residues
MQPQITVSANERLIRRGAATLGCLLAAGWMLGAWATLDRARAQTTVETATAAPQAVPGFWDPRHRPDRPDLSRLTVIRFL
TETDYPPFNYTGPDGNPAGFNVDLARTLCEEIKVTCTIQMRRFETLVDALSSNRGDALIASLAVTPELRKRIDFTDPYYR
TPARFVSRRDAAMAEVRPEYLEGKKVGVVAGSAHEAYLKVFFTDAELHGYPNDETLRQALRQNEVDFIFGDAISLAFWIN
GTDSESCCAFSGGPFIESRYFGEGIGIAVRKGNDVLRQALNWALFRIWERGRYTDLWLRYFSVSPF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=226, Percent_Identity=34.9557522123894, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=29.3333333333333, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=28.2051282051282, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=28.82096069869, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788228, Length=220, Percent_Identity=28.6363636363636, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787031, Length=226, Percent_Identity=27.8761061946903, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 34376; Mature: 34376

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPQITVSANERLIRRGAATLGCLLAAGWMLGAWATLDRARAQTTVETATAAPQAVPGFW
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DPRHRPDRPDLSRLTVIRFLTETDYPPFNYTGPDGNPAGFNVDLARTLCEEIKVTCTIQM
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEEEH
RRFETLVDALSSNRGDALIASLAVTPELRKRIDFTDPYYRTPARFVSRRDAAMAEVRPEY
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
LEGKKVGVVAGSAHEAYLKVFFTDAELHGYPNDETLRQALRQNEVDFIFGDAISLAFWIN
HCCCEEEEEECCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEEEEC
GTDSESCCAFSGGPFIESRYFGEGIGIAVRKGNDVLRQALNWALFRIWERGRYTDLWLRY
CCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHEE
FSVSPF
CCCCCC
>Mature Secondary Structure
MQPQITVSANERLIRRGAATLGCLLAAGWMLGAWATLDRARAQTTVETATAAPQAVPGFW
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DPRHRPDRPDLSRLTVIRFLTETDYPPFNYTGPDGNPAGFNVDLARTLCEEIKVTCTIQM
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEEEH
RRFETLVDALSSNRGDALIASLAVTPELRKRIDFTDPYYRTPARFVSRRDAAMAEVRPEY
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
LEGKKVGVVAGSAHEAYLKVFFTDAELHGYPNDETLRQALRQNEVDFIFGDAISLAFWIN
HCCCEEEEEECCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEEEEC
GTDSESCCAFSGGPFIESRYFGEGIGIAVRKGNDVLRQALNWALFRIWERGRYTDLWLRY
CCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHEE
FSVSPF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]