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The map label for this gene is dapL [H]

Identifier: 75674281

GI number: 75674281

Start: 97354

End: 98625

Strand: Direct

Name: dapL [H]

Synonym: Nwi_0082

Alternate gene names: 75674281

Gene position: 97354-98625 (Clockwise)

Preceding gene: 75674278

Following gene: 75674282

Centisome position: 2.86

GC content: 69.89

Gene sequence:

>1272_bases
ATGGCAACCCCCGCTTCATCGCGCGCGGCGCAAGGCGACGCCAGCCCGACCCAGCTCGACGCCGGCGCGTTCCTGTCCGC
CGCCGGCCCGGCCGCGCTGACGGCTGCAAGCGAGCGGTCGCCATTCGCGCGGACGGCGGAGCTGCTTGCGCCCTACCAGC
CGGAACAATCCCTGATCAACCTGTCGCTGGGCGAGCCGCAGGACCCGCCGCCCGATTTCGTCGGCCCGATCCTGTCCCGG
CATATCGACGAGTTCAGGCGATACCCGATGGCGCGGGGGATCGAGCCGTTCCGCCGCGCCGCCGCCGAATGGCTGTCGCG
GCGCTTCGCGCTGCCGCGCGCGATCGATCCCGATGCCGAGATCCTGGCGCTGAACGGCAGCCGCGAGGGGCTGTTTTACG
CCGCGATCGCCGCCGCGCGCTACGTCGCGCCGCGCAAGGGCGCGCCGGCCATCCTGATGCCGAATCCTTTCTACCCGGCC
TATGGCGCGGGCGCGCGCGCCGCCAATTGCGAAGCCATTTTCCTGCCGGCCACGCGCGCCAACGGCTTCCTGCCCGACCT
CGATTCTCTCGATGACGCCACGCTGGCGCGGACGGTCGCGATCTATCTCGCCTCGCCCGCCAATCCGCAAGGCGCGGTGG
CCTCGCGCGATTACCTCTCGCGCCTGATCGCGCTGGCGCGCCGTCACGGCTTCCTGGTGTTCTCGGACGAATGCTACTCG
GAGATCTACACGCAAAGTCCGCCCGGCAGCGCGCTGGAATGCGCGGGGCCGGACTTCGCCCACGTGGTCGCGTTCCAGTC
GCTCTCCAAACGCTCGAACCTGCCGGGGCTGCGCGTGGGCTTCGTCGCCGGAGACCGGAATTTCCTCACCGCGTTCCATG
AACTGCGCAACGTCGCCGCGCCGCAGGTGCCGGCGCCGCTGCAGCATGTCGGCGCGGCGGCCTATGGCGACGAGGCGCAT
GTGGAGGAGAACCGAAGGCTCTACCGGCTCAAGTTCGATCTCGCCGACGGGATCATCGGGACCCGTTTCGGTTATGCGAG
GCCCGCCGGCGGCTTCTGCCTCTGGCTGGATGTCTCCGCATATGGCGGCGACGAGGCCGCGACGATCAGGCTTTACAGGG
AGGCCGGCCTCCGCGTCGTGCCCGGAAGCTATCTGGCGCGTCCGCAAGGTGACGGCGGCAATCCCGGCGCGGGCTATATC
CGCCTGGCGCTGGTGGCGGACATCGAAACCACGGCCGAGGCGTTGCGTCGGCTGGTGAAAACACTCGGTTAG

Upstream 100 bases:

>100_bases
TTAATCGTGTCTTAACCCCGCCGCAGCTAAGGTTGCTCAGCCCTCTTTCGTCCGGCCTCGACCTGCGGCCGAACGTGTTG
AAAGTATTGATGCTTAACCG

Downstream 100 bases:

>100_bases
TCGCGGGGCACCATGAGCATGGCAGCGATCGAACGTCTTCTTCCCATCGTCGATCAGATACCGCCGTCGATCCGCGAGCC
GCTCGCCCGTCGCCTGCGCG

Product: aminotransferase, class I and II

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MATPASSRAAQGDASPTQLDAGAFLSAAGPAALTAASERSPFARTAELLAPYQPEQSLINLSLGEPQDPPPDFVGPILSR
HIDEFRRYPMARGIEPFRRAAAEWLSRRFALPRAIDPDAEILALNGSREGLFYAAIAAARYVAPRKGAPAILMPNPFYPA
YGAGARAANCEAIFLPATRANGFLPDLDSLDDATLARTVAIYLASPANPQGAVASRDYLSRLIALARRHGFLVFSDECYS
EIYTQSPPGSALECAGPDFAHVVAFQSLSKRSNLPGLRVGFVAGDRNFLTAFHELRNVAAPQVPAPLQHVGAAAYGDEAH
VEENRRLYRLKFDLADGIIGTRFGYARPAGGFCLWLDVSAYGGDEAATIRLYREAGLRVVPGSYLARPQGDGGNPGAGYI
RLALVADIETTAEALRRLVKTLG

Sequences:

>Translated_423_residues
MATPASSRAAQGDASPTQLDAGAFLSAAGPAALTAASERSPFARTAELLAPYQPEQSLINLSLGEPQDPPPDFVGPILSR
HIDEFRRYPMARGIEPFRRAAAEWLSRRFALPRAIDPDAEILALNGSREGLFYAAIAAARYVAPRKGAPAILMPNPFYPA
YGAGARAANCEAIFLPATRANGFLPDLDSLDDATLARTVAIYLASPANPQGAVASRDYLSRLIALARRHGFLVFSDECYS
EIYTQSPPGSALECAGPDFAHVVAFQSLSKRSNLPGLRVGFVAGDRNFLTAFHELRNVAAPQVPAPLQHVGAAAYGDEAH
VEENRRLYRLKFDLADGIIGTRFGYARPAGGFCLWLDVSAYGGDEAATIRLYREAGLRVVPGSYLARPQGDGGNPGAGYI
RLALVADIETTAEALRRLVKTLG
>Mature_422_residues
ATPASSRAAQGDASPTQLDAGAFLSAAGPAALTAASERSPFARTAELLAPYQPEQSLINLSLGEPQDPPPDFVGPILSRH
IDEFRRYPMARGIEPFRRAAAEWLSRRFALPRAIDPDAEILALNGSREGLFYAAIAAARYVAPRKGAPAILMPNPFYPAY
GAGARAANCEAIFLPATRANGFLPDLDSLDDATLARTVAIYLASPANPQGAVASRDYLSRLIALARRHGFLVFSDECYSE
IYTQSPPGSALECAGPDFAHVVAFQSLSKRSNLPGLRVGFVAGDRNFLTAFHELRNVAAPQVPAPLQHVGAAAYGDEAHV
EENRRLYRLKFDLADGIIGTRFGYARPAGGFCLWLDVSAYGGDEAATIRLYREAGLRVVPGSYLARPQGDGGNPGAGYIR
LALVADIETTAEALRRLVKTLG

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788722, Length=386, Percent_Identity=27.2020725388601, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788627, Length=401, Percent_Identity=22.6932668329177, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17567663, Length=409, Percent_Identity=22.7383863080685, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR019881
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.83 [H]

Molecular weight: Translated: 45183; Mature: 45051

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPASSRAAQGDASPTQLDAGAFLSAAGPAALTAASERSPFARTAELLAPYQPEQSLIN
CCCCCCCCCCCCCCCCCEECCCCHHHCCCCHHEEECCCCCCHHHHHHHHCCCCCCHHEEE
LSLGEPQDPPPDFVGPILSRHIDEFRRYPMARGIEPFRRAAAEWLSRRFALPRAIDPDAE
EECCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCE
ILALNGSREGLFYAAIAAARYVAPRKGAPAILMPNPFYPAYGAGARAANCEAIFLPATRA
EEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCC
NGFLPDLDSLDDATLARTVAIYLASPANPQGAVASRDYLSRLIALARRHGFLVFSDECYS
CCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHH
EIYTQSPPGSALECAGPDFAHVVAFQSLSKRSNLPGLRVGFVAGDRNFLTAFHELRNVAA
HHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCC
PQVPAPLQHVGAAAYGDEAHVEENRRLYRLKFDLADGIIGTRFGYARPAGGFCLWLDVSA
CCCCCCHHHHCCHHCCCCHHHCCCCEEEEEEEEHHCCCHHCCCCCCCCCCCEEEEEEEEC
YGGDEAATIRLYREAGLRVVPGSYLARPQGDGGNPGAGYIRLALVADIETTAEALRRLVK
CCCCCCEEEEEEECCCCEECCCHHHCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHH
TLG
HCC
>Mature Secondary Structure 
ATPASSRAAQGDASPTQLDAGAFLSAAGPAALTAASERSPFARTAELLAPYQPEQSLIN
CCCCCCCCCCCCCCCCEECCCCHHHCCCCHHEEECCCCCCHHHHHHHHCCCCCCHHEEE
LSLGEPQDPPPDFVGPILSRHIDEFRRYPMARGIEPFRRAAAEWLSRRFALPRAIDPDAE
EECCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCE
ILALNGSREGLFYAAIAAARYVAPRKGAPAILMPNPFYPAYGAGARAANCEAIFLPATRA
EEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCC
NGFLPDLDSLDDATLARTVAIYLASPANPQGAVASRDYLSRLIALARRHGFLVFSDECYS
CCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHH
EIYTQSPPGSALECAGPDFAHVVAFQSLSKRSNLPGLRVGFVAGDRNFLTAFHELRNVAA
HHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCC
PQVPAPLQHVGAAAYGDEAHVEENRRLYRLKFDLADGIIGTRFGYARPAGGFCLWLDVSA
CCCCCCHHHHCCHHCCCCHHHCCCCEEEEEEEEHHCCCHHCCCCCCCCCCCEEEEEEEEC
YGGDEAATIRLYREAGLRVVPGSYLARPQGDGGNPGAGYIRLALVADIETTAEALRRLVK
CCCCCCEEEEEEECCCCEECCCHHHCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHH
TLG
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA