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The map label for this gene is dnaN [H]

Identifier: 75674201

GI number: 75674201

Start: 2211

End: 3329

Strand: Direct

Name: dnaN [H]

Synonym: Nwi_0002

Alternate gene names: 75674201

Gene position: 2211-3329 (Clockwise)

Preceding gene: 75674200

Following gene: 75674202

Centisome position: 0.06

GC content: 63.18

Gene sequence:

>1119_bases
ATGAAGGTCACCGTCGAACGCGCGCAACTGCTGAAATCGCTCGGCCACGTCCATCGCGTTGTCGAACGGCGGAACACGAT
TCCGATCCTGGGAAACGTGCTGGTCCGCGCCGAGAACGCCAAGCTGTCGCTGAAGGCGACCGATCTCGATCTGGAGGTGA
CGGAAACGCTGGCGGCGGAAACCGGCACTGTGGGCTCGACCACGGTTCCAGCGCATATGTTCTACGACATCATCCGCAAG
CTGCCCGATGGCGCGCAGATCGTGCTGGAAGGCGATGGCGACCGCTCGGTTCTGACGATCCGCGCCGGGCGCTCCCGCTT
CACCCTGCAGACGCTGCCGGAGAATGATTTCCCCGATCTCGCGGCCGGCGAGATGACGCATTCTTTCACATTGGCCGCGG
CAGACCTGAAGCGGCTGATCGATCGTACCCAGTTCGCGATCTCGACCGAAGAGACCCGCTATTATCTCAATGGCATCTAC
CTGCACGGCGCGGGCACCGCAAACAACGCGACGCTGCGCGGCGTCGCGACCGACGGTCATCGCCTAGCGCAACTCGATCT
CGCTTTGCCGAAGGGCGCCGAGGGAATGCCCGGCGTCATCATTCCGCGCAAGACGGTCGGCGAGGTGCAGCGGTTGATCG
AGGATAGCGAGGCCGAGATCGGAATCGAACTGTCGCAAGGCAAGATCCGCTTCACCCTCGGCAACGTCGTGCTGACCTCC
AAGCTGATCGACGGCACCTTCCCGGACTACGGCCGCGTGATCCCGCAGAACAACGACAAGGAACTGGTCGTCGACAAGAA
GGATTTCGAGGCCGCGGTAGATCGCGTCTCGACGATTTCCAGCGAGCGCGGCCGCGCCGTGAAGCTGGCGCTGTCAGCGG
GCAAGCTCGTTCTGTCCGTGACCAATCCCGATTCCGGCAGCGCCACCGAGGAGCTTGAGGTCGAATACGCCTCCGACGCA
CTCGATATCGGATTCAATTCACGCTATCTCCTGGATATCGCCGCGCAGATCGAGGGCGAAGCCGCGGTGCTGAAGCTCGC
CGATCCCGGATCGCCGACGCTGGTGCACGACAGGGATTCCAGGGGCGCGCTCTACGTCCTGATGCCGATGCGGGTGTGA

Upstream 100 bases:

>100_bases
CTTGAGCTTCGAGGCATCGCTTGAACTCCGAGGTCTCGGCGCCGCGAGTCCTCGCGGCATTTTCAGCACACGACCTGAAC
CTGGATCGGACGGATTTGCA

Downstream 100 bases:

>100_bases
GGAATCTCTCGGATCGCGAGGTCTCCAATTTCGCTCTCTCCCCTTGCGGGAGAGGGAAATATTGCCCGGCGTCATGATTC
CCTCCCGCGTTCGCCGCCTG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAKLSLKATDLDLEVTETLAAETGTVGSTTVPAHMFYDIIRK
LPDGAQIVLEGDGDRSVLTIRAGRSRFTLQTLPENDFPDLAAGEMTHSFTLAAADLKRLIDRTQFAISTEETRYYLNGIY
LHGAGTANNATLRGVATDGHRLAQLDLALPKGAEGMPGVIIPRKTVGEVQRLIEDSEAEIGIELSQGKIRFTLGNVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDA
LDIGFNSRYLLDIAAQIEGEAAVLKLADPGSPTLVHDRDSRGALYVLMPMRV

Sequences:

>Translated_372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAKLSLKATDLDLEVTETLAAETGTVGSTTVPAHMFYDIIRK
LPDGAQIVLEGDGDRSVLTIRAGRSRFTLQTLPENDFPDLAAGEMTHSFTLAAADLKRLIDRTQFAISTEETRYYLNGIY
LHGAGTANNATLRGVATDGHRLAQLDLALPKGAEGMPGVIIPRKTVGEVQRLIEDSEAEIGIELSQGKIRFTLGNVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDA
LDIGFNSRYLLDIAAQIEGEAAVLKLADPGSPTLVHDRDSRGALYVLMPMRV
>Mature_372_residues
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAKLSLKATDLDLEVTETLAAETGTVGSTTVPAHMFYDIIRK
LPDGAQIVLEGDGDRSVLTIRAGRSRFTLQTLPENDFPDLAAGEMTHSFTLAAADLKRLIDRTQFAISTEETRYYLNGIY
LHGAGTANNATLRGVATDGHRLAQLDLALPKGAEGMPGVIIPRKTVGEVQRLIEDSEAEIGIELSQGKIRFTLGNVVLTS
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASDA
LDIGFNSRYLLDIAAQIEGEAAVLKLADPGSPTLVHDRDSRGALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=41.0666666666667, Blast_Score=278, Evalue=3e-76,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40281; Mature: 40281

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAKLSLKATDLDLEVTETLAAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEECCCEEHHHHHHHC
TGTVGSTTVPAHMFYDIIRKLPDGAQIVLEGDGDRSVLTIRAGRSRFTLQTLPENDFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCEEEEEECCCCCCCCC
AAGEMTHSFTLAAADLKRLIDRTQFAISTEETRYYLNGIYLHGAGTANNATLRGVATDGH
CCCCCHHEEEEHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCEEEEEECCCC
RLAQLDLALPKGAEGMPGVIIPRKTVGEVQRLIEDSEAEIGIELSQGKIRFTLGNVVLTS
EEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCEEEEE
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSV
HHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVEYASDALDIGFNSRYLLDIAAQIEGEAAVLKLADPGSPTLVHDRDS
ECCCCCCCCCEEEEEECCCEEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCEEECCCC
RGALYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLVRAENAKLSLKATDLDLEVTETLAAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEEECCCEEHHHHHHHC
TGTVGSTTVPAHMFYDIIRKLPDGAQIVLEGDGDRSVLTIRAGRSRFTLQTLPENDFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCEEEEEECCCCCCCCC
AAGEMTHSFTLAAADLKRLIDRTQFAISTEETRYYLNGIYLHGAGTANNATLRGVATDGH
CCCCCHHEEEEHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCCEEEEEECCCC
RLAQLDLALPKGAEGMPGVIIPRKTVGEVQRLIEDSEAEIGIELSQGKIRFTLGNVVLTS
EEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCEEEEE
KLIDGTFPDYGRVIPQNNDKELVVDKKDFEAAVDRVSTISSERGRAVKLALSAGKLVLSV
HHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEE
TNPDSGSATEELEVEYASDALDIGFNSRYLLDIAAQIEGEAAVLKLADPGSPTLVHDRDS
ECCCCCCCCCEEEEEECCCEEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCEEECCCC
RGALYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]