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The map label for this gene is dnaA [H]

Identifier: 75674200

GI number: 75674200

Start: 567

End: 1994

Strand: Direct

Name: dnaA [H]

Synonym: Nwi_0001

Alternate gene names: 75674200

Gene position: 567-1994 (Clockwise)

Preceding gene: NA

Following gene: 75674201

Centisome position: 0.02

GC content: 65.27

Gene sequence:

>1428_bases
ATGTCAGACACGGAACAGGAGCGCTGGTCGCGGGTGAAGGGTCGATTGCGATCGACCGTGGGAGAGGACATCTATTCAAG
CTGGTTTGCGAGAATGGATCTTGAAAGCGTGCATGGCGAGAGCGTTCGCCTGTCGGTCCCGACCCGGTTTCTGAAAAGCT
GGATCCAGGCGCACTACGCCGAGCGCGTGCTGTCCTGCTGGCAAGCTGAATTACCGGACGTTCACCGGATCGATCTCACC
GTGCGTTCGGCCATGCGCTGCGCCGCGCCGGTCCGGGAAGCGCCCGCGACCGATGCGCGCCACCCCGAACGCAGCGAAGG
CCGGAACGGCGTTGAGTTGAAAACGGTTGCGACCGCGCCGGCGTCCGCCAACCATGACGCGCTCGGCGGCTCGCCTCTCG
ACCCGAGGCTGACGTTTCAAAGTTTCGTCACCGGCCGCTCCAATACGCTGGCCCACGCGGCGGCGCGGCAGGTCGCCGAA
GGCCGGCGCGGCGACAGCGTGATGTTCAACCCGCTCTACATTCACGCCGGCGTGGGCCTTGGCAAAACGCATCTGCTGCA
AGCCGTCACATGGGCGGGAAACGCAGGCACGGAGCGCAGGGTGCTCTACCTGACGGCGGAGAAGTTCATGTATGGCTTCG
TCGCCGCGCTGAAGACGCAGACGGCGCTCGCCTTCAAGGAAGCGCTCCGCGGCATCGACGTTCTGGTCATCGACGACCTG
CAGTTCCTGCAAGGCAAATCGACCCAGGCTGAGTTCTGCCACACCTTGAATGCGCTGATCGATGCGGGCCGCCAGGTGGT
CATTGCAGCCGACCGGCCGCCGTCCGATCTGGAAAGCCTCGACGATCGCGTGCGGTCGAGACTCGCCGGCGGGCTCGTGG
TCGAAATGGGATCGCTCGGCGAGGAGCTTCGGCTCGGCATCCTGCGGTCGCGGGTTGAAGCCGCCCGAGTCCACCACGCC
AGTTTCGACGTGCCGGAGCCGGTCCTGGATTACCTCGCGAAAGCGATCACGCATAACGGCCGCGACCTCGAAGGCGCGAT
CAACCGGCTTCTGGCGCACAGCAAGCTCAACGCCCAGCCGGTCACGCTGGAAATGGCCGAGCGCGAGGTCCGTGACCTGG
TCCGTCCGCAGGAACCCCGGCGCATCAAGATCGAGGACATCCAGCGGGTCGTTGCGCGGCAGTACAATGTAAGCCGCTCG
GACCTTCTGTCCTCGCGCCGCACCGCCAACGTGGTGAGGCCGCGGCAGGTCGCGATGTATCTGGCTAAAACGCTGACGTT
GCGTTCCCTGCCCGAGATCGGCCGCCGGTTCGGGGGCCGGGATCACACCACCGTGCTGCACGCCGTGCGCAAGATCGAGG
CGCTGGTGTCGAAGGACACGACGCTCTCCGACGAGGTCGAACTGCTGAAACGCCAGTTGCAGGAATAG

Upstream 100 bases:

>100_bases
CATGACTGAACGAGCGCACGGAAGGATCACGGCGAACGCTTGATCTTCAACAGAGCAGTCGCATCGCAACAACCTTTAAC
CAGAAAAGTCGTCACAAGAC

Downstream 100 bases:

>100_bases
GCGCGGAGCGGGAAAAATCCTTCCGGAAAAAGCCGGACCATGGCGAACCACAGGGGCTTGCGCAGGAAGCACTTCCGCGT
CACCTTACGGCCCCGCCGGA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSDTEQERWSRVKGRLRSTVGEDIYSSWFARMDLESVHGESVRLSVPTRFLKSWIQAHYAERVLSCWQAELPDVHRIDLT
VRSAMRCAAPVREAPATDARHPERSEGRNGVELKTVATAPASANHDALGGSPLDPRLTFQSFVTGRSNTLAHAAARQVAE
GRRGDSVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGTERRVLYLTAEKFMYGFVAALKTQTALAFKEALRGIDVLVIDDL
QFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMGSLGEELRLGILRSRVEAARVHHA
SFDVPEPVLDYLAKAITHNGRDLEGAINRLLAHSKLNAQPVTLEMAEREVRDLVRPQEPRRIKIEDIQRVVARQYNVSRS
DLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVSKDTTLSDEVELLKRQLQE

Sequences:

>Translated_475_residues
MSDTEQERWSRVKGRLRSTVGEDIYSSWFARMDLESVHGESVRLSVPTRFLKSWIQAHYAERVLSCWQAELPDVHRIDLT
VRSAMRCAAPVREAPATDARHPERSEGRNGVELKTVATAPASANHDALGGSPLDPRLTFQSFVTGRSNTLAHAAARQVAE
GRRGDSVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGTERRVLYLTAEKFMYGFVAALKTQTALAFKEALRGIDVLVIDDL
QFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMGSLGEELRLGILRSRVEAARVHHA
SFDVPEPVLDYLAKAITHNGRDLEGAINRLLAHSKLNAQPVTLEMAEREVRDLVRPQEPRRIKIEDIQRVVARQYNVSRS
DLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVSKDTTLSDEVELLKRQLQE
>Mature_474_residues
SDTEQERWSRVKGRLRSTVGEDIYSSWFARMDLESVHGESVRLSVPTRFLKSWIQAHYAERVLSCWQAELPDVHRIDLTV
RSAMRCAAPVREAPATDARHPERSEGRNGVELKTVATAPASANHDALGGSPLDPRLTFQSFVTGRSNTLAHAAARQVAEG
RRGDSVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGTERRVLYLTAEKFMYGFVAALKTQTALAFKEALRGIDVLVIDDLQ
FLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMGSLGEELRLGILRSRVEAARVHHAS
FDVPEPVLDYLAKAITHNGRDLEGAINRLLAHSKLNAQPVTLEMAEREVRDLVRPQEPRRIKIEDIQRVVARQYNVSRSD
LLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVSKDTTLSDEVELLKRQLQE

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=484, Percent_Identity=35.5371900826446, Blast_Score=272, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52925; Mature: 52794

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTEQERWSRVKGRLRSTVGEDIYSSWFARMDLESVHGESVRLSVPTRFLKSWIQAHYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ERVLSCWQAELPDVHRIDLTVRSAMRCAAPVREAPATDARHPERSEGRNGVELKTVATAP
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCEEEEEEECC
ASANHDALGGSPLDPRLTFQSFVTGRSNTLAHAAARQVAEGRRGDSVMFNPLYIHAGVGL
CCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEECCCC
GKTHLLQAVTWAGNAGTERRVLYLTAEKFMYGFVAALKTQTALAFKEALRGIDVLVIDDL
CHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCH
QFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMGSLG
HHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEHHHCC
EELRLGILRSRVEAARVHHASFDVPEPVLDYLAKAITHNGRDLEGAINRLLAHSKLNAQP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
VTLEMAEREVRDLVRPQEPRRIKIEDIQRVVARQYNVSRSDLLSSRRTANVVRPRQVAMY
CHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
LAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVSKDTTLSDEVELLKRQLQE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTEQERWSRVKGRLRSTVGEDIYSSWFARMDLESVHGESVRLSVPTRFLKSWIQAHYA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ERVLSCWQAELPDVHRIDLTVRSAMRCAAPVREAPATDARHPERSEGRNGVELKTVATAP
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCEEEEEEECC
ASANHDALGGSPLDPRLTFQSFVTGRSNTLAHAAARQVAEGRRGDSVMFNPLYIHAGVGL
CCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEECCCC
GKTHLLQAVTWAGNAGTERRVLYLTAEKFMYGFVAALKTQTALAFKEALRGIDVLVIDDL
CHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCH
QFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVEMGSLG
HHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEHHHCC
EELRLGILRSRVEAARVHHASFDVPEPVLDYLAKAITHNGRDLEGAINRLLAHSKLNAQP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
VTLEMAEREVRDLVRPQEPRRIKIEDIQRVVARQYNVSRSDLLSSRRTANVVRPRQVAMY
CHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
LAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKIEALVSKDTTLSDEVELLKRQLQE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA