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The map label for this gene is recF [H]

Identifier: 75674202

GI number: 75674202

Start: 3391

End: 4548

Strand: Direct

Name: recF [H]

Synonym: Nwi_0003

Alternate gene names: 75674202

Gene position: 3391-4548 (Clockwise)

Preceding gene: 75674201

Following gene: 75674203

Centisome position: 0.1

GC content: 70.03

Gene sequence:

>1158_bases
TTGCCCGGCGTCATGATTCCCTCCCGCGTTCGCCGCCTGAGCCTCACCCACTTCCGCAACTACCGCGCCGCGACGTTCGA
GACGCGCAGCAACATGATCGTGCTGGTCGGACCGAACGGTGCCGGCAAGACCAATTGCCTCGAAGCCATTTCGCTGCTGT
CGCCGGGACGCGGATTGCGTCGCGCGACACGCGACGACATCGCGGACAACACCGGAGACGGCTCCTGGGCGGTGTCGGCC
GAGATGCAAGGCGCGCTCGGCCTTGCGACGCTCGGCACCGGCATCGACGCGCCGGGCAATGAAGCGGCTCCAAGCGGCCG
GCGCTGCCGGATCGACCGCGAGCCCGTCGCATCGGCCGCCGCGTTCGGCGACCATCTGCGCATGGTCTGGCTGACGCCCT
CGATGGACGGCCTGTTCACCGGGCCGGCGTCGGACAGGCGGCGTTTCCTCGACCGTCTGGTGCTGGCGATCGACAGTGAA
CACTCCGGCCGCGTCTCGGCGCTGGAGCGCAGCCTGCGCTCGCGCAACCGCCTGCTGGAAATGCGTCACCACGACGATCT
CTGGTGCGAGGCCATTGAGCGTAAAACCGCCGAGCTTGCGGTCGCGGTCGCGGCGATGCGCGCGCAGACCGTGACGCGCC
TTACGGCCGCGCTGGAGGCGCGCGGCGGGGGCTCCTCATTCCCCGCGGCAAGCATCCACCTCGACGGCTGGATGGAGAAC
GCGCTGCTGACCGAGCCGGCCACTGTGGTGGAGGATCGCTACCGCGAAATCCTGCGCGCCAGCCGCCCTCGCGACGCCGC
CGCCGGCCGCACGCTCGAAGGCCCGCATCTCACCGATCTGGAGGTGATCTATGCGCCGAAGAACATGCCGGCGAAGGAGG
CCTCCACAGGCGAGCAGAAGGCGCTCCTGATCGGCCTGGTGCTGGCCCACGCCCGCCTCGTGGCCGAGATGACGGGCATC
ATCCCGCTGCTGCTGCTCGATGAAGTCGTGGCTCACCTCGATCCCGACCGCCGCGGCGCGTTGTTCGGCGAGCTTGCCGG
CCTCGGCGCCCAGGTCTGGATGAGCGGCGCCGACCCGGCCGCCTTCGCCAACCTCAGTGCCGGCAGCGAAACCTTCAGGG
TGGACTCCGGCCGGATCACGCGCGAGCCGCCCACGTAA

Upstream 100 bases:

>100_bases
AGGGGCGCGCTCTACGTCCTGATGCCGATGCGGGTGTGAGGAATCTCTCGGATCGCGAGGTCTCCAATTTCGCTCTCTCC
CCTTGCGGGAGAGGGAAATA

Downstream 100 bases:

>100_bases
TGCCATAGGAACCGCTCGGGACGGGAGCTGGAGAATCCCGCAGCTTTCGTCCCCGAGGCGACGGCTCCGATGGTTGCGAA
TCGCGATGTGGGAAGCTTCG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MPGVMIPSRVRRLSLTHFRNYRAATFETRSNMIVLVGPNGAGKTNCLEAISLLSPGRGLRRATRDDIADNTGDGSWAVSA
EMQGALGLATLGTGIDAPGNEAAPSGRRCRIDREPVASAAAFGDHLRMVWLTPSMDGLFTGPASDRRRFLDRLVLAIDSE
HSGRVSALERSLRSRNRLLEMRHHDDLWCEAIERKTAELAVAVAAMRAQTVTRLTAALEARGGGSSFPAASIHLDGWMEN
ALLTEPATVVEDRYREILRASRPRDAAAGRTLEGPHLTDLEVIYAPKNMPAKEASTGEQKALLIGLVLAHARLVAEMTGI
IPLLLLDEVVAHLDPDRRGALFGELAGLGAQVWMSGADPAAFANLSAGSETFRVDSGRITREPPT

Sequences:

>Translated_385_residues
MPGVMIPSRVRRLSLTHFRNYRAATFETRSNMIVLVGPNGAGKTNCLEAISLLSPGRGLRRATRDDIADNTGDGSWAVSA
EMQGALGLATLGTGIDAPGNEAAPSGRRCRIDREPVASAAAFGDHLRMVWLTPSMDGLFTGPASDRRRFLDRLVLAIDSE
HSGRVSALERSLRSRNRLLEMRHHDDLWCEAIERKTAELAVAVAAMRAQTVTRLTAALEARGGGSSFPAASIHLDGWMEN
ALLTEPATVVEDRYREILRASRPRDAAAGRTLEGPHLTDLEVIYAPKNMPAKEASTGEQKALLIGLVLAHARLVAEMTGI
IPLLLLDEVVAHLDPDRRGALFGELAGLGAQVWMSGADPAAFANLSAGSETFRVDSGRITREPPT
>Mature_384_residues
PGVMIPSRVRRLSLTHFRNYRAATFETRSNMIVLVGPNGAGKTNCLEAISLLSPGRGLRRATRDDIADNTGDGSWAVSAE
MQGALGLATLGTGIDAPGNEAAPSGRRCRIDREPVASAAAFGDHLRMVWLTPSMDGLFTGPASDRRRFLDRLVLAIDSEH
SGRVSALERSLRSRNRLLEMRHHDDLWCEAIERKTAELAVAVAAMRAQTVTRLTAALEARGGGSSFPAASIHLDGWMENA
LLTEPATVVEDRYREILRASRPRDAAAGRTLEGPHLTDLEVIYAPKNMPAKEASTGEQKALLIGLVLAHARLVAEMTGII
PLLLLDEVVAHLDPDRRGALFGELAGLGAQVWMSGADPAAFANLSAGSETFRVDSGRITREPPT

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=377, Percent_Identity=26.790450928382, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41434; Mature: 41303

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGVMIPSRVRRLSLTHFRNYRAATFETRSNMIVLVGPNGAGKTNCLEAISLLSPGRGLR
CCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHH
RATRDDIADNTGDGSWAVSAEMQGALGLATLGTGIDAPGNEAAPSGRRCRIDREPVASAA
HHHHHHHCCCCCCCCEEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHH
AFGDHLRMVWLTPSMDGLFTGPASDRRRFLDRLVLAIDSEHSGRVSALERSLRSRNRLLE
HHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MRHHDDLWCEAIERKTAELAVAVAAMRAQTVTRLTAALEARGGGSSFPAASIHLDGWMEN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHCC
ALLTEPATVVEDRYREILRASRPRDAAAGRTLEGPHLTDLEVIYAPKNMPAKEASTGEQK
CHHCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHH
ALLIGLVLAHARLVAEMTGIIPLLLLDEVVAHLDPDRRGALFGELAGLGAQVWMSGADPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHEEECCCCCH
AFANLSAGSETFRVDSGRITREPPT
HEEECCCCCCEEEECCCEECCCCCC
>Mature Secondary Structure 
PGVMIPSRVRRLSLTHFRNYRAATFETRSNMIVLVGPNGAGKTNCLEAISLLSPGRGLR
CCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHH
RATRDDIADNTGDGSWAVSAEMQGALGLATLGTGIDAPGNEAAPSGRRCRIDREPVASAA
HHHHHHHCCCCCCCCEEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHH
AFGDHLRMVWLTPSMDGLFTGPASDRRRFLDRLVLAIDSEHSGRVSALERSLRSRNRLLE
HHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MRHHDDLWCEAIERKTAELAVAVAAMRAQTVTRLTAALEARGGGSSFPAASIHLDGWMEN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHCC
ALLTEPATVVEDRYREILRASRPRDAAAGRTLEGPHLTDLEVIYAPKNMPAKEASTGEQK
CHHCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHH
ALLIGLVLAHARLVAEMTGIIPLLLLDEVVAHLDPDRRGALFGELAGLGAQVWMSGADPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHEEECCCCCH
AFANLSAGSETFRVDSGRITREPPT
HEEECCCCCCEEEECCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]