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The map label for this gene is hisF [H]

Identifier: 73542789

GI number: 73542789

Start: 3413487

End: 3414257

Strand: Reverse

Name: hisF [H]

Synonym: Reut_A3105

Alternate gene names: 73542789

Gene position: 3414257-3413487 (Counterclockwise)

Preceding gene: 73542790

Following gene: 73542788

Centisome position: 89.69

GC content: 65.89

Gene sequence:

>771_bases
ATGCTAGCCAAACGTATCATCCCCTGCCTCGATGTGACGAACGGGCGGGTGGTCAAGGGCGTCAACTTCGTCGAGCTGCG
CGATGCGGGCGATCCCGTGGAAATCGCGCGACGCTACGACGAGCAGGGCGCCGACGAAATCACTTTTCTCGACATCACCG
CGACCAGCGATGGGCGCGACCTGATCCTGCACATCATCGAGGCGGTGGCCTCGCAGGTCTTTATTCCGCTGACCGTTGGC
GGCGGCGTGCGCGCCGTCGAAGATGTGCGGCGCCTGCTCAACGCCGGTGCGGACAAGATCAGCGTCAATTCGTCGGCGAT
CGCCAATCCGCAACTCGTGTCGGATGCCACCGGCAAGTACGGCTCGCAGTGCATCGTGGTTGCCATCGACGCCAAGCGCA
GCTCGGCGCCAGGCGAGCCCCCGCGCTGGGAAGTTTTTACGCACGGCGGACGCAAGGCAACCGGGCTCGACGCGGTGGAG
TGGGCGAAGGAAATGGCGGCACGTGGCGCGGGCGAGATCCTGCTGACCAGCATGGATCGTGACGGCACCAAGAGCGGTTT
CGACCTGGAACTCACCCGCGCGGTCAGCGATGCGGTGCCGGTGCCGGTAATCGCGTCGGGCGGCGTCGGCGGCCTGCAGG
ACCTGGCCGATGGCATCCGGCTCGGCCACGCGGATGCGGTGCTGGCCGCCAGCATTTTCCACTACGGCCAGCATACGGTT
GGCGAGGCAAAGGCATTCATGGCCCGCGAGGGCATTCCTGTGCGGATCTGA

Upstream 100 bases:

>100_bases
CGCGTGCGGACAAGCTGAGCAACGGGGAATAAGCCAACGCGCCCCGCGGGGCGCTGCCACCGCCAGGCTGCTACCGGTCT
CGCGGCGGACAAGGAATCTC

Downstream 100 bases:

>100_bases
TCATGGCAAAGAAGTGGCTCAACAAGGTCAAGTGGGACGATAACGGCCTGGTGCCGGTGATCGTGCAGGAAGTCGGCACC
AATGACGTGCTGATGTTCGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKYGSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVE
WAKEMAARGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGQHTV
GEAKAFMAREGIPVRI

Sequences:

>Translated_256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKYGSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVE
WAKEMAARGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGQHTV
GEAKAFMAREGIPVRI
>Mature_256_residues
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKYGSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVE
WAKEMAARGAGEILLTSMDRDGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGQHTV
GEAKAFMAREGIPVRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=43.8461538461538, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=30.327868852459, Blast_Score=95, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=162, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26965; Mature: 26965

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKY
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
GSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVEWAKEMAARGAGEILLTSMDR
CCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGQHTV
CCCCCCCCEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
GEAKAFMAREGIPVRI
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTNGRVVKGVNFVELRDAGDPVEIARRYDEQGADEITFLDITATSDGRD
CCHHHCCCCEECCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKISVNSSAIANPQLVSDATGKY
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
GSQCIVVAIDAKRSSAPGEPPRWEVFTHGGRKATGLDAVEWAKEMAARGAGEILLTSMDR
CCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTKSGFDLELTRAVSDAVPVPVIASGGVGGLQDLADGIRLGHADAVLAASIFHYGQHTV
CCCCCCCCEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
GEAKAFMAREGIPVRI
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA