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The map label for this gene is hisI

Identifier: 73542788

GI number: 73542788

Start: 3413077

End: 3413484

Strand: Reverse

Name: hisI

Synonym: Reut_A3104

Alternate gene names: 73542788

Gene position: 3413484-3413077 (Counterclockwise)

Preceding gene: 73542789

Following gene: 73542787

Centisome position: 89.67

GC content: 59.56

Gene sequence:

>408_bases
ATGGCAAAGAAGTGGCTCAACAAGGTCAAGTGGGACGATAACGGCCTGGTGCCGGTGATCGTGCAGGAAGTCGGCACCAA
TGACGTGCTGATGTTCGCGTTTATGAACCGCGATGCGCTGCAGAAGACCGCGGAACTCGGCGAAGCCGTGTTCTGGTCGC
GCTCGCGCAAGCGGCTGTGGCACAAGGGCGAAGAGTCGGGCCACGTGCAGAAGGTGCACGAGATGCGCCTCGACTGTGAC
GAAGACGTGGTACTGCTCAAGGTCACCCAGGTCGACAACATCGCCTGCCACACTGGTCGGCATTCGTGCTTCTTCCAGAA
GTTCGAAGGTGACGCCGAGAGTGGCGACTGGCAGACCGTCGAGCCTGTGCTCAAGGACCCTTCCCAAATCTACACGGCCA
AACCATGA

Upstream 100 bases:

>100_bases
ATGCGGTGCTGGCCGCCAGCATTTTCCACTACGGCCAGCATACGGTTGGCGAGGCAAAGGCATTCATGGCCCGCGAGGGC
ATTCCTGTGCGGATCTGATC

Downstream 100 bases:

>100_bases
CCGAATCCCACTCTGCCGGTACCGACGTGCTGGCGCGCCTGGCTGTGGTGCTGGAGTCGCGCAAGCCTGAGAATGGCGGC
GACCCCGAGAAATCGTACGT

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MAKKWLNKVKWDDNGLVPVIVQEVGTNDVLMFAFMNRDALQKTAELGEAVFWSRSRKRLWHKGEESGHVQKVHEMRLDCD
EDVVLLKVTQVDNIACHTGRHSCFFQKFEGDAESGDWQTVEPVLKDPSQIYTAKP

Sequences:

>Translated_135_residues
MAKKWLNKVKWDDNGLVPVIVQEVGTNDVLMFAFMNRDALQKTAELGEAVFWSRSRKRLWHKGEESGHVQKVHEMRLDCD
EDVVLLKVTQVDNIACHTGRHSCFFQKFEGDAESGDWQTVEPVLKDPSQIYTAKP
>Mature_134_residues
AKKWLNKVKWDDNGLVPVIVQEVGTNDVLMFAFMNRDALQKTAELGEAVFWSRSRKRLWHKGEESGHVQKVHEMRLDCDE
DVVLLKVTQVDNIACHTGRHSCFFQKFEGDAESGDWQTVEPVLKDPSQIYTAKP

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=129, Percent_Identity=40.3100775193798, Blast_Score=87, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319819, Length=105, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_CUPPJ (Q46WL9)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297308.1
- ProteinModelPortal:   Q46WL9
- SMR:   Q46WL9
- GeneID:   3609695
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A3104
- NMPDR:   fig|264198.3.peg.2260
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   PRK00051
- BioCyc:   REUT264198:REUT_A3104-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 15516; Mature: 15385

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKWLNKVKWDDNGLVPVIVQEVGTNDVLMFAFMNRDALQKTAELGEAVFWSRSRKRLW
CCHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
HKGEESGHVQKVHEMRLDCDEDVVLLKVTQVDNIACHTGRHSCFFQKFEGDAESGDWQTV
CCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCEEHHCCCCCCCCCCCCCC
EPVLKDPSQIYTAKP
CHHHCCCCCEEECCC
>Mature Secondary Structure 
AKKWLNKVKWDDNGLVPVIVQEVGTNDVLMFAFMNRDALQKTAELGEAVFWSRSRKRLW
CHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
HKGEESGHVQKVHEMRLDCDEDVVLLKVTQVDNIACHTGRHSCFFQKFEGDAESGDWQTV
CCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCEEHHCCCCCCCCCCCCCC
EPVLKDPSQIYTAKP
CHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA