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The map label for this gene is murE [H]

Identifier: 73542668

GI number: 73542668

Start: 3274193

End: 3275755

Strand: Reverse

Name: murE [H]

Synonym: Reut_A2984

Alternate gene names: 73542668

Gene position: 3275755-3274193 (Counterclockwise)

Preceding gene: 73542669

Following gene: 73542667

Centisome position: 86.06

GC content: 70.95

Gene sequence:

>1563_bases
ATGACGGCCAAGCCGCTGCTCCCGCTCGAAGTCACCGCACAGGCCAGCGACGCGCTGGCCTGGCTGCGTACCAAGGTGCC
GGCCAGTGCCCGGCTGACCGGCGATACGCGCCGCCTGGCGCGCGGCGACGTCTTCCTGGCTTACGTGCTCGGCAACGAGC
GCCTGTCCACCGACGGCCGCCCGCATATCGCGCAGGCCATTGCCGCCGGCGCCGGTGCGATCCTGTATGAGGCCGATGGC
TTCGACTGGACGTTCGGCGACACCGTGCCGCATCTCGCGCTGAACAGCCTGCACCAGCTTGCCGGCCCGATTGCCGCCGG
CTGGTACGGCAATCCCGCTCGCGGCGTGGCCGTCACCGGCATGACCGGCACCAACGGCAAGACTTCGTGCAGCCAGTGGC
TGGCGCGGCTGCTGCAAGCCACCGGTACGCCATGCGCGACCATCGGCACGCTCGGTACCGGCTTCCCGGATGCGCTGCAG
CCGACCGGCTTCACTACGCCCGATGCCGTGCAACTGCAAGCCAGTCTGGCTAGCCTGCACGATGCCGGCGCGCGCGCGGT
GGCAATGGAAGTGTCGTCCCACGGTCTCGAACAGGAGCGCGTGGCCGGTACGCATTTCTCGGTCGCGGTGCTGACCAACC
TGACGCAGGACCACCTCGACTACCACGGCACCATGGCCGAGTACGAGGCCGCCAAAGCGCGCCTGTTCCGCTGGGACGGC
CTGCGCACCGCCGTGATCAATCGCGATGACGGGATGGGGCGGCGCCTGCTGGCGTCCAACGGCGCGGCCATCGCGGCGCC
GCATGTGATCGAGTACGGCATCGACGGTCCGGCGCGTGCGTCCATCAGCGGCGCGCGTGGCCAATGGCTGCGCGCCAGCG
GCGTGCGCGCGACGGCGACCGGCACCGCATTCCACATCGACGGCAGCTTCGGCAGCGCCGATGTCGCCACGCCGATGATC
GGCGCGTTCAATGTCAGCAACCTGCTTGCAGTGCTCGGTGCGGCGCTGGCCAACGGGGTGGCGTGGGATGCGGCGCTCGC
CGCGCTGCGCGCGCTCTCGCCCGTCGATGGCCGCATGGAGCTGTTCGGCGCGCACGCGGGCCAGGACGGCCCGCTGGCCG
TGGTCGACTACGCGCACACGCCCGATGCACTGGAAAAGACGCTGGAAGCGCTGCGCCCGGTTGCGCAGGCACGCAATGGC
CGCCTGTGGTGCGTGTTCGGCTGTGGCGGCGACCGCGACCCGATCAAGCGCCCCCTCATGGGCGGCGTGGCGGAGCGCCT
GGCCGACGAGATCGTGCTGACCAGCGACAACCCGCGCAGCGAGGATCCGCAGGACATCCTCGACGCCATTGCCGACGGCA
TGGCCGACCGCGCGCGCGGTCGCCAGATCGAAGACCGCGCCGCTGCGATCCTCTATGCAATCAAGCACGCGGCCGGTGCC
GACGTGGTGCTGGTGGCCGGCAAGGGCCACGAGGCCACGCAGGAAGCCCATGGCCGCAAGCGGCCGTTCTCGGACCGCGA
GCATGTACGCCTGGCCCTCGCCGCGCGGGGGGTATCGGCATGA

Upstream 100 bases:

>100_bases
GCGGCACGCTGCGTGCGCTGAATGTCCAGCCCGATTCGCCGATCCGCCAACTGGTGGTCAGCGACAAGGTGCAGGAAAGC
GAACCGTGGAGCTCGACGCA

Downstream 100 bases:

>100_bases
GCGATTCCAAGATGACGAATTTGCAGGAAGCCGCCGGCTGGATTGCCGGCGCGCGTGTTCTGGGCGATGGCACGCAGGCG
TTTGCCCGCGTGCATACCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTAKPLLPLEVTAQASDALAWLRTKVPASARLTGDTRRLARGDVFLAYVLGNERLSTDGRPHIAQAIAAGAGAILYEADG
FDWTFGDTVPHLALNSLHQLAGPIAAGWYGNPARGVAVTGMTGTNGKTSCSQWLARLLQATGTPCATIGTLGTGFPDALQ
PTGFTTPDAVQLQASLASLHDAGARAVAMEVSSHGLEQERVAGTHFSVAVLTNLTQDHLDYHGTMAEYEAAKARLFRWDG
LRTAVINRDDGMGRRLLASNGAAIAAPHVIEYGIDGPARASISGARGQWLRASGVRATATGTAFHIDGSFGSADVATPMI
GAFNVSNLLAVLGAALANGVAWDAALAALRALSPVDGRMELFGAHAGQDGPLAVVDYAHTPDALEKTLEALRPVAQARNG
RLWCVFGCGGDRDPIKRPLMGGVAERLADEIVLTSDNPRSEDPQDILDAIADGMADRARGRQIEDRAAAILYAIKHAAGA
DVVLVAGKGHEATQEAHGRKRPFSDREHVRLALAARGVSA

Sequences:

>Translated_520_residues
MTAKPLLPLEVTAQASDALAWLRTKVPASARLTGDTRRLARGDVFLAYVLGNERLSTDGRPHIAQAIAAGAGAILYEADG
FDWTFGDTVPHLALNSLHQLAGPIAAGWYGNPARGVAVTGMTGTNGKTSCSQWLARLLQATGTPCATIGTLGTGFPDALQ
PTGFTTPDAVQLQASLASLHDAGARAVAMEVSSHGLEQERVAGTHFSVAVLTNLTQDHLDYHGTMAEYEAAKARLFRWDG
LRTAVINRDDGMGRRLLASNGAAIAAPHVIEYGIDGPARASISGARGQWLRASGVRATATGTAFHIDGSFGSADVATPMI
GAFNVSNLLAVLGAALANGVAWDAALAALRALSPVDGRMELFGAHAGQDGPLAVVDYAHTPDALEKTLEALRPVAQARNG
RLWCVFGCGGDRDPIKRPLMGGVAERLADEIVLTSDNPRSEDPQDILDAIADGMADRARGRQIEDRAAAILYAIKHAAGA
DVVLVAGKGHEATQEAHGRKRPFSDREHVRLALAARGVSA
>Mature_519_residues
TAKPLLPLEVTAQASDALAWLRTKVPASARLTGDTRRLARGDVFLAYVLGNERLSTDGRPHIAQAIAAGAGAILYEADGF
DWTFGDTVPHLALNSLHQLAGPIAAGWYGNPARGVAVTGMTGTNGKTSCSQWLARLLQATGTPCATIGTLGTGFPDALQP
TGFTTPDAVQLQASLASLHDAGARAVAMEVSSHGLEQERVAGTHFSVAVLTNLTQDHLDYHGTMAEYEAAKARLFRWDGL
RTAVINRDDGMGRRLLASNGAAIAAPHVIEYGIDGPARASISGARGQWLRASGVRATATGTAFHIDGSFGSADVATPMIG
AFNVSNLLAVLGAALANGVAWDAALAALRALSPVDGRMELFGAHAGQDGPLAVVDYAHTPDALEKTLEALRPVAQARNGR
LWCVFGCGGDRDPIKRPLMGGVAERLADEIVLTSDNPRSEDPQDILDAIADGMADRARGRQIEDRAAAILYAIKHAAGAD
VVLVAGKGHEATQEAHGRKRPFSDREHVRLALAARGVSA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=481, Percent_Identity=44.4906444906445, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54358; Mature: 54227

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKPLLPLEVTAQASDALAWLRTKVPASARLTGDTRRLARGDVFLAYVLGNERLSTDGR
CCCCCCCCEEEECCHHHHHHHHHHCCCCCEEECCCHHHHHCCCEEEEEEECCCEECCCCC
PHIAQAIAAGAGAILYEADGFDWTFGDTVPHLALNSLHQLAGPIAAGWYGNPARGVAVTG
HHHHHHHHCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
MTGTNGKTSCSQWLARLLQATGTPCATIGTLGTGFPDALQPTGFTTPDAVQLQASLASLH
CCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
DAGARAVAMEVSSHGLEQERVAGTHFSVAVLTNLTQDHLDYHGTMAEYEAAKARLFRWDG
HCCCEEEEEEHHHCCCCHHHHCCCCEEEHHHHHCHHHHHHHCCCHHHHHHHHHHEEEECC
LRTAVINRDDGMGRRLLASNGAAIAAPHVIEYGIDGPARASISGARGQWLRASGVRATAT
CEEEEEECCCCCCCCHHHCCCCEEECCHHEEECCCCCCCCCCCCCCCCEEECCCCEEEEC
GTAFHIDGSFGSADVATPMIGAFNVSNLLAVLGAALANGVAWDAALAALRALSPVDGRME
CEEEEEECCCCCCHHCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHH
LFGAHAGQDGPLAVVDYAHTPDALEKTLEALRPVAQARNGRLWCVFGCGGDRDPIKRPLM
EEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
GGVAERLADEIVLTSDNPRSEDPQDILDAIADGMADRARGRQIEDRAAAILYAIKHAAGA
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
DVVLVAGKGHEATQEAHGRKRPFSDREHVRLALAARGVSA
CEEEEECCCCHHHHHHCCCCCCCCCHHHEEEEEECCCCCC
>Mature Secondary Structure 
TAKPLLPLEVTAQASDALAWLRTKVPASARLTGDTRRLARGDVFLAYVLGNERLSTDGR
CCCCCCCEEEECCHHHHHHHHHHCCCCCEEECCCHHHHHCCCEEEEEEECCCEECCCCC
PHIAQAIAAGAGAILYEADGFDWTFGDTVPHLALNSLHQLAGPIAAGWYGNPARGVAVTG
HHHHHHHHCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
MTGTNGKTSCSQWLARLLQATGTPCATIGTLGTGFPDALQPTGFTTPDAVQLQASLASLH
CCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
DAGARAVAMEVSSHGLEQERVAGTHFSVAVLTNLTQDHLDYHGTMAEYEAAKARLFRWDG
HCCCEEEEEEHHHCCCCHHHHCCCCEEEHHHHHCHHHHHHHCCCHHHHHHHHHHEEEECC
LRTAVINRDDGMGRRLLASNGAAIAAPHVIEYGIDGPARASISGARGQWLRASGVRATAT
CEEEEEECCCCCCCCHHHCCCCEEECCHHEEECCCCCCCCCCCCCCCCEEECCCCEEEEC
GTAFHIDGSFGSADVATPMIGAFNVSNLLAVLGAALANGVAWDAALAALRALSPVDGRME
CEEEEEECCCCCCHHCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHH
LFGAHAGQDGPLAVVDYAHTPDALEKTLEALRPVAQARNGRLWCVFGCGGDRDPIKRPLM
EEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
GGVAERLADEIVLTSDNPRSEDPQDILDAIADGMADRARGRQIEDRAAAILYAIKHAAGA
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
DVVLVAGKGHEATQEAHGRKRPFSDREHVRLALAARGVSA
CEEEEECCCCHHHHHHCCCCCCCCCHHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]