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The map label for this gene is cspA [H]

Identifier: 73542439

GI number: 73542439

Start: 3025038

End: 3025247

Strand: Direct

Name: cspA [H]

Synonym: Reut_A2754

Alternate gene names: 73542439

Gene position: 3025038-3025247 (Clockwise)

Preceding gene: 73542434

Following gene: 73542442

Centisome position: 79.47

GC content: 57.62

Gene sequence:

>210_bases
ATGGCAAGCGGTATCGTCAAATGGTTCAATGACGCCAAGGGCTTCGGGTTCATCAAGCCGGACGAAGGCGAAGAAGAACT
GTTTGCGCACTTTTCGGCTATCCAGATGGCGGGCTTCAAGACGCTCAAGGAAGGTCAGCGCGTCTCGTTCGAGGTCGTGC
AAGGCCCCAAGGGCAAGCAAGCCACGAACATCCAGGACGCCTCGGCCTGA

Upstream 100 bases:

>100_bases
GCATCCGACAGGAAGCGGCCGCCATGGCGCCGCAGCGCTTGTCATCCGGCTGGCCGCAGGCATCCCCGCTTTGAAATCTG
CAATTTCGTTGGGGGAGTAT

Downstream 100 bases:

>100_bases
AGTGCGCCGCGACCGCACCCGGGCCGACAGAGTTCCGGGGTGGTCACCAAGGATGCGGGGGGAACTCCCCCGGAACGGGA
TCCGCGCAGGTTGTGCCGCC

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MASGIVKWFNDAKGFGFIKPDEGEEELFAHFSAIQMAGFKTLKEGQRVSFEVVQGPKGKQATNIQDASA

Sequences:

>Translated_69_residues
MASGIVKWFNDAKGFGFIKPDEGEEELFAHFSAIQMAGFKTLKEGQRVSFEVVQGPKGKQATNIQDASA
>Mature_68_residues
ASGIVKWFNDAKGFGFIKPDEGEEELFAHFSAIQMAGFKTLKEGQRVSFEVVQGPKGKQATNIQDASA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=62.5, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=62.2950819672131, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=59.6774193548387, Blast_Score=82, Evalue=5e-18,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=60.655737704918, Blast_Score=79, Evalue=7e-17,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=53.2258064516129, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=53.2258064516129, Blast_Score=75, Evalue=6e-16,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=54.8387096774194, Blast_Score=75, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=47.5409836065574, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7522; Mature: 7390

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGIVKWFNDAKGFGFIKPDEGEEELFAHFSAIQMAGFKTLKEGQRVSFEVVQGPKGKQ
CCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ATNIQDASA
CCCCCCCCC
>Mature Secondary Structure 
ASGIVKWFNDAKGFGFIKPDEGEEELFAHFSAIQMAGFKTLKEGQRVSFEVVQGPKGKQ
CCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ATNIQDASA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA