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The map label for this gene is rluE [H]

Identifier: 73542442

GI number: 73542442

Start: 3027592

End: 3028251

Strand: Direct

Name: rluE [H]

Synonym: Reut_A2757

Alternate gene names: 73542442

Gene position: 3027592-3028251 (Clockwise)

Preceding gene: 73542439

Following gene: 73542443

Centisome position: 79.54

GC content: 67.42

Gene sequence:

>660_bases
ATGACGTTGATTGCCCTGAACAAACCCTTTGGCACGATGAGCCAGTTTTCCGAGCATCCCTCTCGCCCAACGCTGGCCGA
GTGGATCGCAACGCCTGGCGTGTACCCGGCAGGCCGGCTTGATGCCGACAGCGAAGGCCTGCTGCTGCTGACCGATGACG
GCGGCCTGCAGGCGCGCATTGCCGATCCGCGGCACAAGCTAGCCAAGACCTACTTCGCGCAGGTCGAGGGGATTCCCGAC
GAGGCCGCGCTGGCGCGCCTGCGACAGGGCATCGACCTCGGCGACTTCCACACCTTGCCGGCCAAGGTACGCGCGGTCGA
GGAGCCGTCATGGCTTTGGCCCCGAAACCCACCGGTGCGCTTTCGCGCCGCGATTCCGACCAGTTGGCTGGAGCTCCAGA
TCCGTGAAGGCAAGAATCGCCAGGTTCGCCGCATGACGGCGGCCGTCGGCTTCCCGACGCTGCGCCTGCTGCGCGCCACC
ATCGGGCGGCTGGATCTACGTGAACTGGGCCTGCAGCCCGGACAGTCGTGCATCGTGGAGCCGGACCGGCTGGGCCTGCC
ACCGGCTCGCACACCGCGCCCGGAGCCGCGAACCCTCGGGTCGGGCAACGCCGGAACGCGCGGCACCGGCATCAAGGGTC
GAGTGCGTTACAAAAACTAA

Upstream 100 bases:

>100_bases
TTCATATTATGCAGCAATTTTCCGTGATCTGCCATTCCGCCAAGCCGCGCCAGTCAAGGAAAGCAGGCGATTGGCCCCGT
CTGCGGCATACTGCTGCCCC

Downstream 100 bases:

>100_bases
CACCCGGCACGTCAACGCACCGGGCGGTGTCCTCGTTGTTGTCGGTGACGCGCCCAATTGCGCGCCGACCTTTCCGACTA
CAGTCACCCTTCGAGGTAAT

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTLIALNKPFGTMSQFSEHPSRPTLAEWIATPGVYPAGRLDADSEGLLLLTDDGGLQARIADPRHKLAKTYFAQVEGIPD
EAALARLRQGIDLGDFHTLPAKVRAVEEPSWLWPRNPPVRFRAAIPTSWLELQIREGKNRQVRRMTAAVGFPTLRLLRAT
IGRLDLRELGLQPGQSCIVEPDRLGLPPARTPRPEPRTLGSGNAGTRGTGIKGRVRYKN

Sequences:

>Translated_219_residues
MTLIALNKPFGTMSQFSEHPSRPTLAEWIATPGVYPAGRLDADSEGLLLLTDDGGLQARIADPRHKLAKTYFAQVEGIPD
EAALARLRQGIDLGDFHTLPAKVRAVEEPSWLWPRNPPVRFRAAIPTSWLELQIREGKNRQVRRMTAAVGFPTLRLLRAT
IGRLDLRELGLQPGQSCIVEPDRLGLPPARTPRPEPRTLGSGNAGTRGTGIKGRVRYKN
>Mature_218_residues
TLIALNKPFGTMSQFSEHPSRPTLAEWIATPGVYPAGRLDADSEGLLLLTDDGGLQARIADPRHKLAKTYFAQVEGIPDE
AALARLRQGIDLGDFHTLPAKVRAVEEPSWLWPRNPPVRFRAAIPTSWLELQIREGKNRQVRRMTAAVGFPTLRLLRATI
GRLDLRELGLQPGQSCIVEPDRLGLPPARTPRPEPRTLGSGNAGTRGTGIKGRVRYKN

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=168, Percent_Identity=55.952380952381, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1788510, Length=174, Percent_Identity=33.9080459770115, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787524, Length=173, Percent_Identity=30.635838150289, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790453, Length=167, Percent_Identity=27.5449101796407, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24164; Mature: 24033

Theoretical pI: Translated: 11.05; Mature: 11.05

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIALNKPFGTMSQFSEHPSRPTLAEWIATPGVYPAGRLDADSEGLLLLTDDGGLQARI
CEEEEECCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEE
ADPRHKLAKTYFAQVEGIPDEAALARLRQGIDLGDFHTLPAKVRAVEEPSWLWPRNPPVR
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHEEEECCCCCCCCCCCCEE
FRAAIPTSWLELQIREGKNRQVRRMTAAVGFPTLRLLRATIGRLDLRELGLQPGQSCIVE
EEEECCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEC
PDRLGLPPARTPRPEPRTLGSGNAGTRGTGIKGRVRYKN
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
TLIALNKPFGTMSQFSEHPSRPTLAEWIATPGVYPAGRLDADSEGLLLLTDDGGLQARI
EEEEECCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEE
ADPRHKLAKTYFAQVEGIPDEAALARLRQGIDLGDFHTLPAKVRAVEEPSWLWPRNPPVR
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHEEEECCCCCCCCCCCCEE
FRAAIPTSWLELQIREGKNRQVRRMTAAVGFPTLRLLRATIGRLDLRELGLQPGQSCIVE
EEEECCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEC
PDRLGLPPARTPRPEPRTLGSGNAGTRGTGIKGRVRYKN
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]