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The map label for this gene is dut [H]

Identifier: 73542434

GI number: 73542434

Start: 3019371

End: 3019871

Strand: Direct

Name: dut [H]

Synonym: Reut_A2749

Alternate gene names: 73542434

Gene position: 3019371-3019871 (Clockwise)

Preceding gene: 73542433

Following gene: 73542439

Centisome position: 79.32

GC content: 66.67

Gene sequence:

>501_bases
ATGAGCCAACAACTGAACCCGACCGTTGAAATCAAGGTCCTCGACGCCCGCCTGAACGAGTGGGGCCTGCCCGCCTACCA
GAGCGACATGGCCGCCGCCATCGACCTGCACGCCTGCGTCGATGCGCCCGTGTCCATCGCCGCGGGCACCGCTGCGCAAC
TGGTGCCGGCCGGCATCGCCGTGCATATGGGCAACCCGTACATGGCCGCGACCATCGTGCCGCGCTCGGGGCTGGGCCAC
AAGAAGGGGCTGGTGCTCGGCAATTCGATTGGCGTGATCGATGCCGATTACCAGGGCCAGATCATGGTCAGCGTGTGGAA
CCGCAACGCGCCGGGTACCGAGCCGATCGTGATTCAGCCCGGAGAGCGCATCGCGCAGATGATGTTTGTGCCCGTGCTGC
GCCCGGTGTTCACGACGGTAGAAGAGTTCTCGGAGAGCAGCGAGCGCGGCGCCGGCGGTTTCGGCTCGACAGGCGTGCAC
CACGCCAAGGCCAGCGCCTGA

Upstream 100 bases:

>100_bases
ACGTACGAACTGCTCGCCGAAGCGTTCGAACTGAATTCCGAAAACTGATGTCCGGCCCGGGCAAGCTGCCCGGCCTGCGA
CTGACTGAACCGATCCAGAA

Downstream 100 bases:

>100_bases
GCGCCGGCTGGCTTCAGTGCATCAGTCCTGCTGCTCGAACAGCAGGGCCACACCTCCGGCGGCATCCGCCGGAATCAGTG
TTTCATGCGGCCCCAAGTCC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSQQLNPTVEIKVLDARLNEWGLPAYQSDMAAAIDLHACVDAPVSIAAGTAAQLVPAGIAVHMGNPYMAATIVPRSGLGH
KKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIVIQPGERIAQMMFVPVLRPVFTTVEEFSESSERGAGGFGSTGVH
HAKASA

Sequences:

>Translated_166_residues
MSQQLNPTVEIKVLDARLNEWGLPAYQSDMAAAIDLHACVDAPVSIAAGTAAQLVPAGIAVHMGNPYMAATIVPRSGLGH
KKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIVIQPGERIAQMMFVPVLRPVFTTVEEFSESSERGAGGFGSTGVH
HAKASA
>Mature_165_residues
SQQLNPTVEIKVLDARLNEWGLPAYQSDMAAAIDLHACVDAPVSIAAGTAAQLVPAGIAVHMGNPYMAATIVPRSGLGHK
KGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIVIQPGERIAQMMFVPVLRPVFTTVEEFSESSERGAGGFGSTGVHH
AKASA

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=51.6556291390728, Blast_Score=164, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71988561, Length=167, Percent_Identity=34.7305389221557, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319729, Length=149, Percent_Identity=33.5570469798658, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921126, Length=151, Percent_Identity=34.4370860927152, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24583610, Length=151, Percent_Identity=34.4370860927152, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 17388; Mature: 17257

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQLNPTVEIKVLDARLNEWGLPAYQSDMAAAIDLHACVDAPVSIAAGTAAQLVPAGIA
CCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHCCCCEE
VHMGNPYMAATIVPRSGLGHKKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIVIQP
EECCCCEEEEEEECCCCCCCCCCEEECCCCCEEECCCCCEEEEEEECCCCCCCCCEEECC
GERIAQMMFVPVLRPVFTTVEEFSESSERGAGGFGSTGVHHAKASA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQQLNPTVEIKVLDARLNEWGLPAYQSDMAAAIDLHACVDAPVSIAAGTAAQLVPAGIA
CCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHCCCCEE
VHMGNPYMAATIVPRSGLGHKKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIVIQP
EECCCCEEEEEEECCCCCCCCCCEEECCCCCEEECCCCCEEEEEEECCCCCCCCCEEECC
GERIAQMMFVPVLRPVFTTVEEFSESSERGAGGFGSTGVHHAKASA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA