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The map label for this gene is dadA1 [H]

Identifier: 73542232

GI number: 73542232

Start: 2790669

End: 2791973

Strand: Direct

Name: dadA1 [H]

Synonym: Reut_A2547

Alternate gene names: 73542232

Gene position: 2790669-2791973 (Clockwise)

Preceding gene: 73542229

Following gene: 73542241

Centisome position: 73.31

GC content: 66.51

Gene sequence:

>1305_bases
ATGCGCGTTCTCGTACTTGGTAGTGGCGTTATCGGCGTCACCAGTGCGTGGTATCTGGCCAAAGCCGGTCACGAAGTGAC
CGTTGTCGATCGCGAGGCGGGGCCCGCGCTTGGCACCAGCTTTGCCAATGCCGGGCAGATCTCGCCCGGCTATGCGTCGC
CATGGGCGGCTCCTGGCGTGCCTCTGAAGGCGATCAAGTGGATGTTCCAGGAACATGCCCCACTGTCGATCCGCCCGGAC
GGGACCCTGTTCCAGTTGCAGTGGATGTGGCAAATGCTGCAGAACTGCAGCGCCGAGCGCTACGCGGTCAACAAGGAGCG
CATGGTCCGTCTGGCCGAATACAGCCGCGACTGCATCCGGGCGCTGCGCGCCGACACCGGCATTGCGTATGAAGGCCGCC
AGCACGGGACGCTGCAAGTTTTCCGCACGCAGGACCAGCTTGATGGCGCCGCCAAGGACATCGCGGTGCTTGAAGAAGCC
GGCGTGCCCTACCAGTTGCTTTCGCGCGAAGAACTCGCCGCGAGCGAACCGGCGCTGGCCGCCGTCAGCCACAAGCTGAC
CGGAGGCCTGCGCCTGCCCAACGATGAAACCGGCGACTGCCAGCTGTTTACCCGACAGCTGGCAGCCATGGCCGAGGCGC
TGGGCGTGAAATTCCAGTACAACCGTTCGATCAACAGCCTCATGACCCAGGGCGACGCCGTAACCGGCGCCGTGGTGGAC
GGCGAGCCGATCGCGGCCGACCTCGTCGTGGTAGCACTCGGCAGCTGGTCGACGCAGCTCGTCAAGCCGTTCCTGCATGG
CATGTCGAACCTGCCGGTCTATCCGCTCAAAGGCTTCTCAATCACGGTGCCGATGACTGACGCCGCGCGCAGCCCGGTGT
CGACCGTGCTCGACGAAACCTACAAGGTGGCGATCACGCGTTTCGAGGACCGCATTCGCGTCGGCGGCATGGCGCAGATC
GTTGGCTACGACCGCTCGCTCGATCCGGCCAAGCGCCGCACGCTCGAGCACGTCGTGACCGACCTGTTCCCGGGCGCCGG
CGACGTCAGCGAGGCCACCTTCTGGACCGGCCTGCGCCCAATGACGCCGGACGGCACACCGATCGTCGGCCCGACTCAGG
TGCGCGGCCTGTGGCTGAACACGGGTCACGGCACCCTTGGCTGGACCATGGCGTGCGGCTCGGGCAAGTTGCTGTCCGAC
CTGGTATCGGGCCGCTCGCCCGCGATCCGCGCCGATGACCTGTCAGTCGGGCGCTACCTGAAGCCGCAGCGCATGCAGGG
CGCCCCGCGTCCCGCTACGGCTTGA

Upstream 100 bases:

>100_bases
TGTTTTCCTGAGAAATTCAATAACTATGACTGGATTAGTTTCAATAGTATAGAGACAAATCCTTCAATACCCTGACATCC
TGGTTAGGAGCCCCCGAAAC

Downstream 100 bases:

>100_bases
CCTTCGGCGCAGCGCGCCACACCAGCAAAAAGGGCGACCCGACGGGCCGCCCTTTTTATTTGCAGAGAACCGCCGGATCA
GAACTGATCGGGCGAGACAG

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRVLVLGSGVIGVTSAWYLAKAGHEVTVVDREAGPALGTSFANAGQISPGYASPWAAPGVPLKAIKWMFQEHAPLSIRPD
GTLFQLQWMWQMLQNCSAERYAVNKERMVRLAEYSRDCIRALRADTGIAYEGRQHGTLQVFRTQDQLDGAAKDIAVLEEA
GVPYQLLSREELAASEPALAAVSHKLTGGLRLPNDETGDCQLFTRQLAAMAEALGVKFQYNRSINSLMTQGDAVTGAVVD
GEPIAADLVVVALGSWSTQLVKPFLHGMSNLPVYPLKGFSITVPMTDAARSPVSTVLDETYKVAITRFEDRIRVGGMAQI
VGYDRSLDPAKRRTLEHVVTDLFPGAGDVSEATFWTGLRPMTPDGTPIVGPTQVRGLWLNTGHGTLGWTMACGSGKLLSD
LVSGRSPAIRADDLSVGRYLKPQRMQGAPRPATA

Sequences:

>Translated_434_residues
MRVLVLGSGVIGVTSAWYLAKAGHEVTVVDREAGPALGTSFANAGQISPGYASPWAAPGVPLKAIKWMFQEHAPLSIRPD
GTLFQLQWMWQMLQNCSAERYAVNKERMVRLAEYSRDCIRALRADTGIAYEGRQHGTLQVFRTQDQLDGAAKDIAVLEEA
GVPYQLLSREELAASEPALAAVSHKLTGGLRLPNDETGDCQLFTRQLAAMAEALGVKFQYNRSINSLMTQGDAVTGAVVD
GEPIAADLVVVALGSWSTQLVKPFLHGMSNLPVYPLKGFSITVPMTDAARSPVSTVLDETYKVAITRFEDRIRVGGMAQI
VGYDRSLDPAKRRTLEHVVTDLFPGAGDVSEATFWTGLRPMTPDGTPIVGPTQVRGLWLNTGHGTLGWTMACGSGKLLSD
LVSGRSPAIRADDLSVGRYLKPQRMQGAPRPATA
>Mature_434_residues
MRVLVLGSGVIGVTSAWYLAKAGHEVTVVDREAGPALGTSFANAGQISPGYASPWAAPGVPLKAIKWMFQEHAPLSIRPD
GTLFQLQWMWQMLQNCSAERYAVNKERMVRLAEYSRDCIRALRADTGIAYEGRQHGTLQVFRTQDQLDGAAKDIAVLEEA
GVPYQLLSREELAASEPALAAVSHKLTGGLRLPNDETGDCQLFTRQLAAMAEALGVKFQYNRSINSLMTQGDAVTGAVVD
GEPIAADLVVVALGSWSTQLVKPFLHGMSNLPVYPLKGFSITVPMTDAARSPVSTVLDETYKVAITRFEDRIRVGGMAQI
VGYDRSLDPAKRRTLEHVVTDLFPGAGDVSEATFWTGLRPMTPDGTPIVGPTQVRGLWLNTGHGTLGWTMACGSGKLLSD
LVSGRSPAIRADDLSVGRYLKPQRMQGAPRPATA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=419, Percent_Identity=67.3031026252983, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 46803; Mature: 46803

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVLGSGVIGVTSAWYLAKAGHEVTVVDREAGPALGTSFANAGQISPGYASPWAAPGV
CEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLKAIKWMFQEHAPLSIRPDGTLFQLQWMWQMLQNCSAERYAVNKERMVRLAEYSRDCIR
CHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHH
ALRADTGIAYEGRQHGTLQVFRTQDQLDGAAKDIAVLEEAGVPYQLLSREELAASEPALA
HHHHCCCCEECCCCCCEEEEEECHHHHCCHHHHHHHHHHCCCCHHHHCHHHHHCCCCHHH
AVSHKLTGGLRLPNDETGDCQLFTRQLAAMAEALGVKFQYNRSINSLMTQGDAVTGAVVD
HHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCEEEEEEC
GEPIAADLVVVALGSWSTQLVKPFLHGMSNLPVYPLKGFSITVPMTDAARSPVSTVLDET
CCCCHHEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHH
YKVAITRFEDRIRVGGMAQIVGYDRSLDPAKRRTLEHVVTDLFPGAGDVSEATFWTGLRP
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCC
MTPDGTPIVGPTQVRGLWLNTGHGTLGWTMACGSGKLLSDLVSGRSPAIRADDLSVGRYL
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCEECCCCCCCCCC
KPQRMQGAPRPATA
CCHHHCCCCCCCCC
>Mature Secondary Structure
MRVLVLGSGVIGVTSAWYLAKAGHEVTVVDREAGPALGTSFANAGQISPGYASPWAAPGV
CEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLKAIKWMFQEHAPLSIRPDGTLFQLQWMWQMLQNCSAERYAVNKERMVRLAEYSRDCIR
CHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHH
ALRADTGIAYEGRQHGTLQVFRTQDQLDGAAKDIAVLEEAGVPYQLLSREELAASEPALA
HHHHCCCCEECCCCCCEEEEEECHHHHCCHHHHHHHHHHCCCCHHHHCHHHHHCCCCHHH
AVSHKLTGGLRLPNDETGDCQLFTRQLAAMAEALGVKFQYNRSINSLMTQGDAVTGAVVD
HHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCEEEEEEC
GEPIAADLVVVALGSWSTQLVKPFLHGMSNLPVYPLKGFSITVPMTDAARSPVSTVLDET
CCCCHHEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHH
YKVAITRFEDRIRVGGMAQIVGYDRSLDPAKRRTLEHVVTDLFPGAGDVSEATFWTGLRP
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCC
MTPDGTPIVGPTQVRGLWLNTGHGTLGWTMACGSGKLLSDLVSGRSPAIRADDLSVGRYL
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCEECCCCCCCCCC
KPQRMQGAPRPATA
CCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]