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The map label for this gene is dmlR [H]

Identifier: 73542229

GI number: 73542229

Start: 2787555

End: 2788463

Strand: Direct

Name: dmlR [H]

Synonym: Reut_A2544

Alternate gene names: 73542229

Gene position: 2787555-2788463 (Clockwise)

Preceding gene: 73542228

Following gene: 73542232

Centisome position: 73.23

GC content: 67.66

Gene sequence:

>909_bases
ATGTACTTCGACCTGCTCGACGTCGCGGTCTTTACGCGCGCGGCGTCGCTGGGCAACCTGTCCGCCGCCGCGCGCGACCT
GCAACTGTCCACCTCCACGGCGAGCGCGCGCCTCGCCAGGCTCGAACAAACCCTCGGCACACGCCTGCTGCACCGGACCA
CGCGCCGGTTGTCGCTGACCGCCGATGGCGAGCGCTTCATCGAACACGCGCAGCAGCTGCTAGCCACCGCCGAGGCCGCC
GAACAGTCGGTCGGCAAAGGGGCGCGGGCACCGCACGGGTTGCTGCGCGTCACCGCGCCAGCTTCGTTCGGGCGGCAGCA
TGTGTCGCCGGCCATTCCGGCTTTTCTCGAAACGTATCCTGAGATACGGCTCGACCTCCGCCTCAGCGATCAGGTCGTGC
CGCTTGTCGACGCAGGCATCGACGTGGCGTTGCGCATGGGCGCTTTGCCAGACTCCTCGCTCGTGGCCCGCGCGCTTGCG
CCAAGCCGCCGGGTGATCTGTGCGGCGCCCTCTTACCTCGCCGCGCACGGCATCCCAAAGCATCCCGATGACCTGCGCAC
GCACAATTGCCTCATTCTCGGCGACCAGTCCGCCTGGCGCTTCGACACGCCGCTCGGGGAAACCGCGGTCACCGTGCATG
GCAACCTGCGCGTGGACAACGGCGAAGTCATCCGCGATGCACTGGTGGCAGGCATGGGCATCGCGCTCAAGTCGACCTGG
GATGTGGGCGCCCTGCTGCGAAGCGGACAACTCGTGACCGTGCTCGACGAGTATCCGGTCCTGCCGGCCGTTTCCATCTG
GGCGGTCTATCCGAGCCGCCATCTTGTTCCCGCCAAGACACGAGCCTTCGTCGACTTCTTTGCGGCACGCTTCGGCAATC
CGCCCTACTGGGACCGGGACCGCGCCTGA

Upstream 100 bases:

>100_bases
GATGGCCGGACTGGCCATCAGCTTCCAGACCTTCTTCGAGTCGCAGGTCGAAGCGGCACGCGCGCTGTCCCAGTAGCCAT
TCCAGCGGCGTCGGGCAGCC

Downstream 100 bases:

>100_bases
GACGCCACGCCACACGGCGCTGGCGCTTCTGGCAATGTCCAGTGCGATCCCCTTCCAACTTGTCCGCGCGCAAACCCCGC
CGATGTCAGGTTCCTCTTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MYFDLLDVAVFTRAASLGNLSAAARDLQLSTSTASARLARLEQTLGTRLLHRTTRRLSLTADGERFIEHAQQLLATAEAA
EQSVGKGARAPHGLLRVTAPASFGRQHVSPAIPAFLETYPEIRLDLRLSDQVVPLVDAGIDVALRMGALPDSSLVARALA
PSRRVICAAPSYLAAHGIPKHPDDLRTHNCLILGDQSAWRFDTPLGETAVTVHGNLRVDNGEVIRDALVAGMGIALKSTW
DVGALLRSGQLVTVLDEYPVLPAVSIWAVYPSRHLVPAKTRAFVDFFAARFGNPPYWDRDRA

Sequences:

>Translated_302_residues
MYFDLLDVAVFTRAASLGNLSAAARDLQLSTSTASARLARLEQTLGTRLLHRTTRRLSLTADGERFIEHAQQLLATAEAA
EQSVGKGARAPHGLLRVTAPASFGRQHVSPAIPAFLETYPEIRLDLRLSDQVVPLVDAGIDVALRMGALPDSSLVARALA
PSRRVICAAPSYLAAHGIPKHPDDLRTHNCLILGDQSAWRFDTPLGETAVTVHGNLRVDNGEVIRDALVAGMGIALKSTW
DVGALLRSGQLVTVLDEYPVLPAVSIWAVYPSRHLVPAKTRAFVDFFAARFGNPPYWDRDRA
>Mature_302_residues
MYFDLLDVAVFTRAASLGNLSAAARDLQLSTSTASARLARLEQTLGTRLLHRTTRRLSLTADGERFIEHAQQLLATAEAA
EQSVGKGARAPHGLLRVTAPASFGRQHVSPAIPAFLETYPEIRLDLRLSDQVVPLVDAGIDVALRMGALPDSSLVARALA
PSRRVICAAPSYLAAHGIPKHPDDLRTHNCLILGDQSAWRFDTPLGETAVTVHGNLRVDNGEVIRDALVAGMGIALKSTW
DVGALLRSGQLVTVLDEYPVLPAVSIWAVYPSRHLVPAKTRAFVDFFAARFGNPPYWDRDRA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=38.9078498293515, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=34.006734006734, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=35.8108108108108, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=28.7671232876712, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.3793103448276, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=29.1666666666667, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=26.643598615917, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790262, Length=247, Percent_Identity=28.3400809716599, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788706, Length=205, Percent_Identity=27.3170731707317, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790011, Length=296, Percent_Identity=23.9864864864865, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=24.8031496062992, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787879, Length=158, Percent_Identity=31.6455696202532, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786448, Length=247, Percent_Identity=28.3400809716599, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32722; Mature: 32722

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFDLLDVAVFTRAASLGNLSAAARDLQLSTSTASARLARLEQTLGTRLLHRTTRRLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEE
ADGERFIEHAQQLLATAEAAEQSVGKGARAPHGLLRVTAPASFGRQHVSPAIPAFLETYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHCHHHCCHHHHHHHHCCC
EIRLDLRLSDQVVPLVDAGIDVALRMGALPDSSLVARALAPSRRVICAAPSYLAAHGIPK
CEEEEEEECCCEEEHHHCCCHHEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHCCCCC
HPDDLRTHNCLILGDQSAWRFDTPLGETAVTVHGNLRVDNGEVIRDALVAGMGIALKSTW
CCCCCCCCCEEEEECCCCEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCEEEECCC
DVGALLRSGQLVTVLDEYPVLPAVSIWAVYPSRHLVPAKTRAFVDFFAARFGNPPYWDRD
CHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
RA
CC
>Mature Secondary Structure
MYFDLLDVAVFTRAASLGNLSAAARDLQLSTSTASARLARLEQTLGTRLLHRTTRRLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEE
ADGERFIEHAQQLLATAEAAEQSVGKGARAPHGLLRVTAPASFGRQHVSPAIPAFLETYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHCHHHCCHHHHHHHHCCC
EIRLDLRLSDQVVPLVDAGIDVALRMGALPDSSLVARALAPSRRVICAAPSYLAAHGIPK
CEEEEEEECCCEEEHHHCCCHHEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHCCCCC
HPDDLRTHNCLILGDQSAWRFDTPLGETAVTVHGNLRVDNGEVIRDALVAGMGIALKSTW
CCCCCCCCCEEEEECCCCEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHCCCEEEECCC
DVGALLRSGQLVTVLDEYPVLPAVSIWAVYPSRHLVPAKTRAFVDFFAARFGNPPYWDRD
CHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
RA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]