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The map label for this gene is ldcA [H]

Identifier: 73541534

GI number: 73541534

Start: 2021221

End: 2022177

Strand: Reverse

Name: ldcA [H]

Synonym: Reut_A1845

Alternate gene names: 73541534

Gene position: 2022177-2021221 (Counterclockwise)

Preceding gene: 73541535

Following gene: 73541533

Centisome position: 53.12

GC content: 65.73

Gene sequence:

>957_bases
ATGAGCACACCGAGCCCCAAGCCGCATACCGAAGTTCGCCTGATCGCGTCGTCCGGCTACCCGCACGATGTCGCCATTGC
GGCGCGCGGCTGTGCGTGGCTCAAGTCGCACGGGTATCACACGACCAACCCCGATGTGCTGGCGCGGCGCTACCTGCGCT
TCGGCGGTACCGATGCGGAACGGCTTTCGGACCTGCATGCGATCGGCACCGGTGCGCCGCATGAACTGACGCTGGCCGTA
CGCGGCGGCTACGGCATTGCGCGACTGCTGGACCGGCTGGATTTCGCGCAGATTGCCGAACAGGCGCGCGCAACCGCCAC
GCCCATCGTCGGTCACAGCGACTTCACGGCGTTCCAGCTCGCCTATCTGGCCGCAACTGGCGGCGTGACGTTTGCCGGCC
CGATGCTCCTCGCCGACTTTGGCGCCGACACGGTCGATCCGTTCATGTGGGAGCACTTCGAGGGCGTGCTGCGCGACCCG
GCCTACACGGTGACTGTGCTGGCACCGCAAGATGACGGCCAGCCGTTCTCGGGCACGGTCACGGGCACGCTGTGGGGCGG
CAACCTGGCCATGCTGTGCAGCCTGCTCGGCACGCCGTTCATGCCGCGGGTGGAAGGCGGCATCTTGTTCCTTGAAGACA
TCAACGAGCCGCCTTACCGCGTGGAGCGCATGTTGCTGCAATTGTTCCAGGCCGGCGTGCTTGGTACGCAGCGCGCGATC
ATCCTTGGAGATTTCTCCAACTACCGCGTGACGGATTACGACAATGGCTACGACATGGACGCCGTCGTTGCTTATCTGCG
CGACCAGTTGCACGTGCCGGTGCTGACAGGCCTGCCGTTCGGTCACTGCGCGCGCAAGCTGACGCTGCCGGTCGGTGGGC
ACGCGCAGCTCACTGCAAGTGCGGAAGGCTTCACGCTGGTCATGTCAGGCTATCCGGTGCTTCCGCCGCTGGCCTGA

Upstream 100 bases:

>100_bases
CTTCGGTGCCCGGCGCCGCGCGCAGAAGGCGGCGCAACTCGCTGCCCGGACGCTGTCCGATCCCGAACAATCCTGATCCC
CCGGACGATCACGAATCCGC

Downstream 100 bases:

>100_bases
CAGCATGTGCCGCCGTGCCATCCGGTCGCCGGAAAGGCAGGCGGATGGTAAAATAATCGGTTATTTCCCCATTTCCTGCC
GGGTTCTCCCATGAACCGGG

Product: L,D-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase A; Muramoyltetrapeptide carboxypeptidase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSTPSPKPHTEVRLIASSGYPHDVAIAARGCAWLKSHGYHTTNPDVLARRYLRFGGTDAERLSDLHAIGTGAPHELTLAV
RGGYGIARLLDRLDFAQIAEQARATATPIVGHSDFTAFQLAYLAATGGVTFAGPMLLADFGADTVDPFMWEHFEGVLRDP
AYTVTVLAPQDDGQPFSGTVTGTLWGGNLAMLCSLLGTPFMPRVEGGILFLEDINEPPYRVERMLLQLFQAGVLGTQRAI
ILGDFSNYRVTDYDNGYDMDAVVAYLRDQLHVPVLTGLPFGHCARKLTLPVGGHAQLTASAEGFTLVMSGYPVLPPLA

Sequences:

>Translated_318_residues
MSTPSPKPHTEVRLIASSGYPHDVAIAARGCAWLKSHGYHTTNPDVLARRYLRFGGTDAERLSDLHAIGTGAPHELTLAV
RGGYGIARLLDRLDFAQIAEQARATATPIVGHSDFTAFQLAYLAATGGVTFAGPMLLADFGADTVDPFMWEHFEGVLRDP
AYTVTVLAPQDDGQPFSGTVTGTLWGGNLAMLCSLLGTPFMPRVEGGILFLEDINEPPYRVERMLLQLFQAGVLGTQRAI
ILGDFSNYRVTDYDNGYDMDAVVAYLRDQLHVPVLTGLPFGHCARKLTLPVGGHAQLTASAEGFTLVMSGYPVLPPLA
>Mature_317_residues
STPSPKPHTEVRLIASSGYPHDVAIAARGCAWLKSHGYHTTNPDVLARRYLRFGGTDAERLSDLHAIGTGAPHELTLAVR
GGYGIARLLDRLDFAQIAEQARATATPIVGHSDFTAFQLAYLAATGGVTFAGPMLLADFGADTVDPFMWEHFEGVLRDPA
YTVTVLAPQDDGQPFSGTVTGTLWGGNLAMLCSLLGTPFMPRVEGGILFLEDINEPPYRVERMLLQLFQAGVLGTQRAII
LGDFSNYRVTDYDNGYDMDAVVAYLRDQLHVPVLTGLPFGHCARKLTLPVGGHAQLTASAEGFTLVMSGYPVLPPLA

Specific function: Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso- Dap-D-Ala. Can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc- tetrapeptide, G

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=302, Percent_Identity=49.3377483443709, Blast_Score=284, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34242; Mature: 34111

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPSPKPHTEVRLIASSGYPHDVAIAARGCAWLKSHGYHTTNPDVLARRYLRFGGTDAE
CCCCCCCCCCEEEEEECCCCCCEEEEEHHCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
RLSDLHAIGTGAPHELTLAVRGGYGIARLLDRLDFAQIAEQARATATPIVGHSDFTAFQL
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCEECCCCCHHHHH
AYLAATGGVTFAGPMLLADFGADTVDPFMWEHFEGVLRDPAYTVTVLAPQDDGQPFSGTV
HHHHHCCCCEEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEE
TGTLWGGNLAMLCSLLGTPFMPRVEGGILFLEDINEPPYRVERMLLQLFQAGVLGTQRAI
EEEEECCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEE
ILGDFSNYRVTDYDNGYDMDAVVAYLRDQLHVPVLTGLPFGHCARKLTLPVGGHAQLTAS
EEECCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHEEECCCCCCEEEEEC
AEGFTLVMSGYPVLPPLA
CCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
STPSPKPHTEVRLIASSGYPHDVAIAARGCAWLKSHGYHTTNPDVLARRYLRFGGTDAE
CCCCCCCCCEEEEEECCCCCCEEEEEHHCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
RLSDLHAIGTGAPHELTLAVRGGYGIARLLDRLDFAQIAEQARATATPIVGHSDFTAFQL
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCEECCCCCHHHHH
AYLAATGGVTFAGPMLLADFGADTVDPFMWEHFEGVLRDPAYTVTVLAPQDDGQPFSGTV
HHHHHCCCCEEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEE
TGTLWGGNLAMLCSLLGTPFMPRVEGGILFLEDINEPPYRVERMLLQLFQAGVLGTQRAI
EEEEECCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEE
ILGDFSNYRVTDYDNGYDMDAVVAYLRDQLHVPVLTGLPFGHCARKLTLPVGGHAQLTAS
EEECCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHEEECCCCCCEEEEEC
AEGFTLVMSGYPVLPPLA
CCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]