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The map label for this gene is menB [C]

Identifier: 73541511

GI number: 73541511

Start: 1996019

End: 1996828

Strand: Reverse

Name: menB [C]

Synonym: Reut_A1822

Alternate gene names: 73541511

Gene position: 1996828-1996019 (Counterclockwise)

Preceding gene: 73541518

Following gene: 73541510

Centisome position: 52.46

GC content: 66.79

Gene sequence:

>810_bases
ATGCGTTACGAAGACTTCCAGTTCATCCTGTTCGACCGGCAGCCGGATGGCGTCCTGCTCGCCACCCTGAACCGGCCCGA
GGTGATGAACGCGACCAACGCCCGGCTTCACTGGGAACTGACGAAGCTGTGGGGGGTCGTCAATGAAGATCCGTCGGTCA
AGGTGGTGGTGGTGACGGGCGCGGGCGACCGTGCGTTCTCGGCGGGCGGCGATCTGGCGTGGGTCGAGGAGATGGTGGGT
AACCCCGATGCGGTGGCGGTCGTAAAGAAGGAGGCCGCCGAGATCGTCTACAACATGCTGGCCTGCGAGAAGCCGATCAT
TTCGGCGATCAACGGCACCGCCGTCGGCGCGGGGCTTGCGGTCGCGCTGCTGGCGGACGTCAGCATCATTGCCGAATCGG
CGCGATTCACGGATGGCCATGCCCGCCTCGGCGTGGCGGCGGGCGACCATGCCGCCATCATCTGGCCGCTGCTCTGTGGT
GTGGCCAAGGCGAAGTACTACCTGATGACCGCGGACTTCATCGACGGCAAGGAAGCTGAGCGCCTGGGGCTGGTGACGTT
CTGCGTGCCACAGCAGGCACTGATGGACCGCGCGCTGGCCGTGGCCCAGTCGCTCGCGCGCGGCAGCCAGACGGCGATTC
GCGCCACCAAGATGTCGATCAACAACTGGATGCGGATGGCCGGGCCGATCTTCGACGCCTCGCTGGCCGCGGAGATGCTG
GGCTTCCTCGGCGCAGACGCGCGCGAAGGCCTCGACGCCGTGCGCAACAAGCGCGCACCGCAATTCCCGTCGGCGAAGCT
GCCTGGCTGA

Upstream 100 bases:

>100_bases
TCTTCCAGAGTACGGAACATCGGTCCGGCCGCTATTGGAGCCATCGAGCCAACCGCGTGTAATGGGGCACGTTGCCCCAT
CATTTTCAGGACATTCCACC

Downstream 100 bases:

>100_bases
GTGACACGGCCTTAAGTCGACATTGCGGCGATCTTGAGCCATCGCCCCGAGAGATTTTGAAGCGAGACATGATGGATACC
GAAGCCAACACCTGGAAGCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRYEDFQFILFDRQPDGVLLATLNRPEVMNATNARLHWELTKLWGVVNEDPSVKVVVVTGAGDRAFSAGGDLAWVEEMVG
NPDAVAVVKKEAAEIVYNMLACEKPIISAINGTAVGAGLAVALLADVSIIAESARFTDGHARLGVAAGDHAAIIWPLLCG
VAKAKYYLMTADFIDGKEAERLGLVTFCVPQQALMDRALAVAQSLARGSQTAIRATKMSINNWMRMAGPIFDASLAAEML
GFLGADAREGLDAVRNKRAPQFPSAKLPG

Sequences:

>Translated_269_residues
MRYEDFQFILFDRQPDGVLLATLNRPEVMNATNARLHWELTKLWGVVNEDPSVKVVVVTGAGDRAFSAGGDLAWVEEMVG
NPDAVAVVKKEAAEIVYNMLACEKPIISAINGTAVGAGLAVALLADVSIIAESARFTDGHARLGVAAGDHAAIIWPLLCG
VAKAKYYLMTADFIDGKEAERLGLVTFCVPQQALMDRALAVAQSLARGSQTAIRATKMSINNWMRMAGPIFDASLAAEML
GFLGADAREGLDAVRNKRAPQFPSAKLPG
>Mature_269_residues
MRYEDFQFILFDRQPDGVLLATLNRPEVMNATNARLHWELTKLWGVVNEDPSVKVVVVTGAGDRAFSAGGDLAWVEEMVG
NPDAVAVVKKEAAEIVYNMLACEKPIISAINGTAVGAGLAVALLADVSIIAESARFTDGHARLGVAAGDHAAIIWPLLCG
VAKAKYYLMTADFIDGKEAERLGLVTFCVPQQALMDRALAVAQSLARGSQTAIRATKMSINNWMRMAGPIFDASLAAEML
GFLGADAREGLDAVRNKRAPQFPSAKLPG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=263, Percent_Identity=27.7566539923954, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=26.6355140186916, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=26.6355140186916, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=26.6355140186916, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37594469, Length=175, Percent_Identity=25.7142857142857, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4502327, Length=270, Percent_Identity=27.037037037037, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI37594471, Length=175, Percent_Identity=25.7142857142857, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI70995211, Length=214, Percent_Identity=28.9719626168224, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=26.615969581749, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=32.9457364341085, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI221142681, Length=200, Percent_Identity=34, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=31.1538461538462, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=266, Percent_Identity=29.6992481203008, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=29.1828793774319, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=213, Percent_Identity=24.4131455399061, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=205, Percent_Identity=27.3170731707317, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=216, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=229, Percent_Identity=26.6375545851528, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=219, Percent_Identity=25.1141552511416, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=27.9527559055118, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=27.9527559055118, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24654903, Length=189, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24653139, Length=270, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=204, Percent_Identity=25.9803921568627, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=23.1075697211155, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19920382, Length=215, Percent_Identity=27.4418604651163, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28743; Mature: 28743

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYEDFQFILFDRQPDGVLLATLNRPEVMNATNARLHWELTKLWGVVNEDPSVKVVVVTG
CCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEEEEC
AGDRAFSAGGDLAWVEEMVGNPDAVAVVKKEAAEIVYNMLACEKPIISAINGTAVGAGLA
CCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHH
VALLADVSIIAESARFTDGHARLGVAAGDHAAIIWPLLCGVAKAKYYLMTADFIDGKEAE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCHH
RLGLVTFCVPQQALMDRALAVAQSLARGSQTAIRATKMSINNWMRMAGPIFDASLAAEML
HEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GFLGADAREGLDAVRNKRAPQFPSAKLPG
HHHCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRYEDFQFILFDRQPDGVLLATLNRPEVMNATNARLHWELTKLWGVVNEDPSVKVVVVTG
CCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEEEEC
AGDRAFSAGGDLAWVEEMVGNPDAVAVVKKEAAEIVYNMLACEKPIISAINGTAVGAGLA
CCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHH
VALLADVSIIAESARFTDGHARLGVAAGDHAAIIWPLLCGVAKAKYYLMTADFIDGKEAE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCHH
RLGLVTFCVPQQALMDRALAVAQSLARGSQTAIRATKMSINNWMRMAGPIFDASLAAEML
HEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GFLGADAREGLDAVRNKRAPQFPSAKLPG
HHHCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA