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The map label for this gene is rbsB [H]

Identifier: 73541341

GI number: 73541341

Start: 1779690

End: 1780658

Strand: Reverse

Name: rbsB [H]

Synonym: Reut_A1651

Alternate gene names: 73541341

Gene position: 1780658-1779690 (Counterclockwise)

Preceding gene: 73541342

Following gene: 73541340

Centisome position: 46.78

GC content: 67.49

Gene sequence:

>969_bases
GTGATCAAGCAATTCCTGATGACAGTCGCGCTGGGCGCATGCTGCATGACCGGGGCCATGGCGCAGGCGCCGGCCAAAGA
CAGCGCGACGACCGCTGCGCCGCGGCAGCTGAAGAAGGTGGGCGTCACGCTGGGCTCGCTCGGCAATCCCTACTTCGTGG
CGCTGGCCCGCGGCGCCGAAGCAGCCGCGAAGCAGGTCAACCCGCAGGCCAAGGTCACCGTGCTGTCGGCCGACTATGAC
CTCAACAAGCAGTTCTCGCACATCGATAGCTTCATCGTCGCCGGCGTGGACCTGATCCTGATCAACGCGGCCGATGCGCG
CGCCATCGAGCCGGCCGTGCGCAAGGCGAAGAAGGCAGGCATCGTGGTGGTCGCGGTGGACGTGGCCGCAGCCGGCGCCG
ACGCCACCGTGCAGACCGACAACATCCAGGCCGGCGAACTCGCTTGCGGTTTCATCGCCGAGCAACTCAAGGGCCATGGC
AACGTCATCATCCAGAACGGGCCGCCGGTGTCGGCGGTGCTGGATCGGGTCAGGGGCTGCAAGACGGTGCTGGCCAAGCA
CCAGGACATCCGCATCCTGTCCGACGACCAGGACGGCAAGGGATCGCGCGAAGGCGGCCTCAATGTCATGCAGCTCTACC
TGACGCGCTTTCCCAAGGTGGATGCGGTCTTCACCATCAATGACCCGCAGGCCGTCGGCGCCGATCTGGCCGCCCGGCAG
CTCAACCGGCGCGGCATCCTGATCGCTTCGGTCGATGGCGCGCCCGACATCGAGGCCGCGCTGAAGGCCGACACGCTCGT
GCAAGCTTCGGCCAGCCAGGATCCGTGGGCCATCGCCCGCACGGCAGTGCAGCTGGGCGTCGGCATGATGAACGGCCAGC
GGCCCGCCAAACCGGTGGTGCTGCTGCCCTCCACGCTCGTCACGCGCGCCAATGTCCGCGATTACAAGGGCTGGGCGACC
CCGAGATAG

Upstream 100 bases:

>100_bases
CAATACATCATCAAGGGTCTGGTCATCATCGGCGCGGTGGCGCTGGACCGCTATCGCCAGAAGGGCTCGGCCCGGACCTG
AACCCGCACAGGAGACGCAA

Downstream 100 bases:

>100_bases
TGCGCGCCCTGCCATACCCCGAACACCTTTCTGACCGTCCCAGGTGACATGATGGATAGCTCCAAGACCGGCGCGCAGGC
CCCGGATTTCGACTTCGTAT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MIKQFLMTVALGACCMTGAMAQAPAKDSATTAAPRQLKKVGVTLGSLGNPYFVALARGAEAAAKQVNPQAKVTVLSADYD
LNKQFSHIDSFIVAGVDLILINAADARAIEPAVRKAKKAGIVVVAVDVAAAGADATVQTDNIQAGELACGFIAEQLKGHG
NVIIQNGPPVSAVLDRVRGCKTVLAKHQDIRILSDDQDGKGSREGGLNVMQLYLTRFPKVDAVFTINDPQAVGADLAARQ
LNRRGILIASVDGAPDIEAALKADTLVQASASQDPWAIARTAVQLGVGMMNGQRPAKPVVLLPSTLVTRANVRDYKGWAT
PR

Sequences:

>Translated_322_residues
MIKQFLMTVALGACCMTGAMAQAPAKDSATTAAPRQLKKVGVTLGSLGNPYFVALARGAEAAAKQVNPQAKVTVLSADYD
LNKQFSHIDSFIVAGVDLILINAADARAIEPAVRKAKKAGIVVVAVDVAAAGADATVQTDNIQAGELACGFIAEQLKGHG
NVIIQNGPPVSAVLDRVRGCKTVLAKHQDIRILSDDQDGKGSREGGLNVMQLYLTRFPKVDAVFTINDPQAVGADLAARQ
LNRRGILIASVDGAPDIEAALKADTLVQASASQDPWAIARTAVQLGVGMMNGQRPAKPVVLLPSTLVTRANVRDYKGWAT
PR
>Mature_322_residues
MIKQFLMTVALGACCMTGAMAQAPAKDSATTAAPRQLKKVGVTLGSLGNPYFVALARGAEAAAKQVNPQAKVTVLSADYD
LNKQFSHIDSFIVAGVDLILINAADARAIEPAVRKAKKAGIVVVAVDVAAAGADATVQTDNIQAGELACGFIAEQLKGHG
NVIIQNGPPVSAVLDRVRGCKTVLAKHQDIRILSDDQDGKGSREGGLNVMQLYLTRFPKVDAVFTINDPQAVGADLAARQ
LNRRGILIASVDGAPDIEAALKADTLVQASASQDPWAIARTAVQLGVGMMNGQRPAKPVVLLPSTLVTRANVRDYKGWAT
PR

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=271, Percent_Identity=30.2583025830258, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1790674, Length=191, Percent_Identity=29.3193717277487, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789990, Length=269, Percent_Identity=29.368029739777, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790526, Length=275, Percent_Identity=24.3636363636364, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33693; Mature: 33693

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQFLMTVALGACCMTGAMAQAPAKDSATTAAPRQLKKVGVTLGSLGNPYFVALARGAE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHH
AAAKQVNPQAKVTVLSADYDLNKQFSHIDSFIVAGVDLILINAADARAIEPAVRKAKKAG
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCC
IVVVAVDVAAAGADATVQTDNIQAGELACGFIAEQLKGHGNVIIQNGPPVSAVLDRVRGC
EEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
KTVLAKHQDIRILSDDQDGKGSREGGLNVMQLYLTRFPKVDAVFTINDPQAVGADLAARQ
HHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
LNRRGILIASVDGAPDIEAALKADTLVQASASQDPWAIARTAVQLGVGMMNGQRPAKPVV
HCCCCEEEEECCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEE
LLPSTLVTRANVRDYKGWATPR
ECCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIKQFLMTVALGACCMTGAMAQAPAKDSATTAAPRQLKKVGVTLGSLGNPYFVALARGAE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHH
AAAKQVNPQAKVTVLSADYDLNKQFSHIDSFIVAGVDLILINAADARAIEPAVRKAKKAG
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCC
IVVVAVDVAAAGADATVQTDNIQAGELACGFIAEQLKGHGNVIIQNGPPVSAVLDRVRGC
EEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
KTVLAKHQDIRILSDDQDGKGSREGGLNVMQLYLTRFPKVDAVFTINDPQAVGADLAARQ
HHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
LNRRGILIASVDGAPDIEAALKADTLVQASASQDPWAIARTAVQLGVGMMNGQRPAKPVV
HCCCCEEEEECCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEE
LLPSTLVTRANVRDYKGWATPR
ECCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]