Click here to switch to the map view.

The map label for this gene is paaJ [H]

Identifier: 73541255

GI number: 73541255

Start: 1685747

End: 1686904

Strand: Direct

Name: paaJ [H]

Synonym: Reut_A1565

Alternate gene names: 73541255

Gene position: 1685747-1686904 (Clockwise)

Preceding gene: 73541254

Following gene: 73541256

Centisome position: 44.29

GC content: 69.17

Gene sequence:

>1158_bases
ATGACCATGAAACACGCCTATATCGTTGACGCCCTGCGCACGCCCACCGGCCGCCGCAAGGGCGGACTGTCGCACATGCA
CGGCGCGGACCTCGGCGGCTTCGTGCTGGCCGAGCTGGTGCGCCGCAACGACATTCCCGCCGAGGACTATGACGACGTGG
TGTTCGGCTGCGTCGACACCATTGGCCCGCTGGCCGGCAATATCGCCCGCAGCGCGTGGCTGGCCGCCGGCCTGCCGCTG
ACCGTGCCGGGCGTGACGGTGGACCGCCAGTGCGGTTCGTCGCAGCAGGCCGTGCACTTTGCCGCGCAGGCGGTGATGAG
CGGCACGCAGGACGTCGTGATCGCCGGCGGCGTGCAGACCATGACGCAGATCCCGATCTCGTCGGCCATGCTGGCAGGGC
AGGCGTTGGGGTTCTCCGACCCGTTCTCGGGTAGCAAGGGCTGGCACGCGCGTTTCGGCGATGCGCCGGTGTCGCAGTTC
CACGCCGCGCAGCGCATCGCCGAGCACTGGAAGCTGTCGCGCGAGGCCATGGAAGCGTACGCGCTGGAAAGCCACCGCCG
CGCCGCGGCTGCGATCGAGGCCGGCCACTTCGCGCGGGAAATCGTGCCGTGCGAAGGCGTGAAGCATGACGAGACACCGC
GCCCCGATACCACGCTGCCCAAGATGGCCGCGCTGGAGCCTCTGATGCCGGGCAGCGCGCTGACCGCGGCTGTATCAAGC
CAGACTGCCGATTCGGCCGCCGCGCTGCTGATCGTTTCCGAAGACGCGCTCAAACGCTACCAGCTCACGCCGCGCGCACG
CATTGCGCATATGAGCGTCATGGGCGACGACCCGCTGTGGATGCTGACCGCGCCGATCCCGGCGACGAAGCGGGCGCTCG
AACGCAGCGGCCTGAGCCTGGCCGACATCGACGTGGTGGAGATCAATGAAGCCTTTGCCTCGGTGGCGATGGCCTGGCTG
GCGGAGACCGGCTACTCCGCCGCGCGCACCAATCCCAACGGAGGCGCCATCGCGCTGGGCCATCCGCTCGGCGCCACCGG
CGCACGCCTGATGACCACGCTGCTGCATGAACTGGAGCGCACCGGCGGCCGCTACGGCCTGCAGACCATGTGCGAAGGCG
GCGGCCTGGCGAACGTCACCATCATCGAACGACTGTAA

Upstream 100 bases:

>100_bases
CCTTCCACAAGGCGCGCCTGGGCGCGCACATCCTGGATGGCGCGCATGACATCGGCGCCGGCGCCACCTTTGCCTGAATC
CGAAGAGAGTCCCAAGAGAC

Downstream 100 bases:

>100_bases
GAGACCGTTTCAAAAAGAGCCCCGGGGGGCTGTTAACCTTTTCGGCAAGCTGTTAACCTTGGCCATCTGAACAACTGGAA
CCGAGGGGATGAGCGCGCCG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTMKHAYIVDALRTPTGRRKGGLSHMHGADLGGFVLAELVRRNDIPAEDYDDVVFGCVDTIGPLAGNIARSAWLAAGLPL
TVPGVTVDRQCGSSQQAVHFAAQAVMSGTQDVVIAGGVQTMTQIPISSAMLAGQALGFSDPFSGSKGWHARFGDAPVSQF
HAAQRIAEHWKLSREAMEAYALESHRRAAAAIEAGHFAREIVPCEGVKHDETPRPDTTLPKMAALEPLMPGSALTAAVSS
QTADSAAALLIVSEDALKRYQLTPRARIAHMSVMGDDPLWMLTAPIPATKRALERSGLSLADIDVVEINEAFASVAMAWL
AETGYSAARTNPNGGAIALGHPLGATGARLMTTLLHELERTGGRYGLQTMCEGGGLANVTIIERL

Sequences:

>Translated_385_residues
MTMKHAYIVDALRTPTGRRKGGLSHMHGADLGGFVLAELVRRNDIPAEDYDDVVFGCVDTIGPLAGNIARSAWLAAGLPL
TVPGVTVDRQCGSSQQAVHFAAQAVMSGTQDVVIAGGVQTMTQIPISSAMLAGQALGFSDPFSGSKGWHARFGDAPVSQF
HAAQRIAEHWKLSREAMEAYALESHRRAAAAIEAGHFAREIVPCEGVKHDETPRPDTTLPKMAALEPLMPGSALTAAVSS
QTADSAAALLIVSEDALKRYQLTPRARIAHMSVMGDDPLWMLTAPIPATKRALERSGLSLADIDVVEINEAFASVAMAWL
AETGYSAARTNPNGGAIALGHPLGATGARLMTTLLHELERTGGRYGLQTMCEGGGLANVTIIERL
>Mature_384_residues
TMKHAYIVDALRTPTGRRKGGLSHMHGADLGGFVLAELVRRNDIPAEDYDDVVFGCVDTIGPLAGNIARSAWLAAGLPLT
VPGVTVDRQCGSSQQAVHFAAQAVMSGTQDVVIAGGVQTMTQIPISSAMLAGQALGFSDPFSGSKGWHARFGDAPVSQFH
AAQRIAEHWKLSREAMEAYALESHRRAAAAIEAGHFAREIVPCEGVKHDETPRPDTTLPKMAALEPLMPGSALTAAVSSQ
TADSAAALLIVSEDALKRYQLTPRARIAHMSVMGDDPLWMLTAPIPATKRALERSGLSLADIDVVEINEAFASVAMAWLA
ETGYSAARTNPNGGAIALGHPLGATGARLMTTLLHELERTGGRYGLQTMCEGGGLANVTIIERL

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=394, Percent_Identity=37.5634517766497, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI167614485, Length=391, Percent_Identity=36.0613810741688, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=34.4139650872818, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=33.587786259542, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI4504327, Length=434, Percent_Identity=32.258064516129, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI194353979, Length=380, Percent_Identity=28.4210526315789, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1787663, Length=403, Percent_Identity=43.6724565756824, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=39.5989974937343, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI87082165, Length=398, Percent_Identity=38.9447236180905, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=37.9652605459057, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=31.7191283292978, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=407, Percent_Identity=35.1351351351351, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=29.7229219143577, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25147385, Length=403, Percent_Identity=30.272952853598, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=418, Percent_Identity=30.1435406698565, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17535917, Length=390, Percent_Identity=26.9230769230769, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=32.9238329238329, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322031, Length=314, Percent_Identity=32.8025477707006, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24655093, Length=398, Percent_Identity=36.1809045226131, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=33.8422391857506, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=30.050505050505, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=30.3529411764706, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 40648; Mature: 40517

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKHAYIVDALRTPTGRRKGGLSHMHGADLGGFVLAELVRRNDIPAEDYDDVVFGCVDT
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
IGPLAGNIARSAWLAAGLPLTVPGVTVDRQCGSSQQAVHFAAQAVMSGTQDVVIAGGVQT
HHHHHHHHHHHHHHHCCCCEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCHHH
MTQIPISSAMLAGQALGFSDPFSGSKGWHARFGDAPVSQFHAAQRIAEHWKLSREAMEAY
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALESHRRAAAAIEAGHFAREIVPCEGVKHDETPRPDTTLPKMAALEPLMPGSALTAAVSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
QTADSAAALLIVSEDALKRYQLTPRARIAHMSVMGDDPLWMLTAPIPATKRALERSGLSL
CCCCCCEEEEEEEHHHHHHHCCCCCHHEEEEEEECCCCEEEEECCCCHHHHHHHHCCCCE
ADIDVVEINEAFASVAMAWLAETGYSAARTNPNGGAIALGHPLGATGARLMTTLLHELER
ECEEEEHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
TGGRYGLQTMCEGGGLANVTIIERL
CCCCCCHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
TMKHAYIVDALRTPTGRRKGGLSHMHGADLGGFVLAELVRRNDIPAEDYDDVVFGCVDT
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
IGPLAGNIARSAWLAAGLPLTVPGVTVDRQCGSSQQAVHFAAQAVMSGTQDVVIAGGVQT
HHHHHHHHHHHHHHHCCCCEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCHHH
MTQIPISSAMLAGQALGFSDPFSGSKGWHARFGDAPVSQFHAAQRIAEHWKLSREAMEAY
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALESHRRAAAAIEAGHFAREIVPCEGVKHDETPRPDTTLPKMAALEPLMPGSALTAAVSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
QTADSAAALLIVSEDALKRYQLTPRARIAHMSVMGDDPLWMLTAPIPATKRALERSGLSL
CCCCCCEEEEEEEHHHHHHHCCCCCHHEEEEEEECCCCEEEEECCCCHHHHHHHHCCCCE
ADIDVVEINEAFASVAMAWLAETGYSAARTNPNGGAIALGHPLGATGARLMTTLLHELER
ECEEEEHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
TGGRYGLQTMCEGGGLANVTIIERL
CCCCCCHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]