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The map label for this gene is rutF [H]

Identifier: 73541234

GI number: 73541234

Start: 1664407

End: 1664931

Strand: Direct

Name: rutF [H]

Synonym: Reut_A1544

Alternate gene names: 73541234

Gene position: 1664407-1664931 (Clockwise)

Preceding gene: 73541233

Following gene: 73541235

Centisome position: 43.73

GC content: 66.67

Gene sequence:

>525_bases
ATGAGCGCCAATCCCATTCCCGGCGCCACGCTGCGCGACGACATGCTGATGGCCATGCGCCGCATGGCGCGCTCGGTGGC
GGTCATCAGCTGCCGCGATGGCGAGCGCCGCTATTCGATGTCCGTGACCGCGGTCGATTCGCTGTCCGCGGATCCGCCGT
CGCTGCTGATCTGCATCAACCGCAATTCATCGATCTACCCCCCGCTCGATGCCGGCGCGGACTTCTGCATCAACCTGCTT
GCCGCCGATCATCGCGATATCGCGGTGGACTGCAGCGGCAGGCTCAAGGGCGAAGAGCGCTTTACCAGCGGGCAGTGGGA
CGATGACATGCTCGGCGTGCCGTGCCTGCGCGACGCCCAGGCCAACCTGATCTGCACGCAGGACGGCCGCTTCGACTATG
GCACGCACGCGGTCTTTATCGGCCGGCTTCGCGAGGTGCGGCTGGCGGGCGAGGTGTCGCCGCTGGTCTATGTCGACGGG
GCCTATACCACTTCCGCGCCGCTCGCCTCGTTGTGTCCGGCGTAA

Upstream 100 bases:

>100_bases
GACCCGCCATGACTTTCGGCTTTATCGCCGCCGACGTGCTGGCGCAGCAAGCGCGGCAGACTGCCGCGGCGCCCCACACT
CAATCCGTTCAGGAGGCCTT

Downstream 100 bases:

>100_bases
TCCATATGTAAGCGGCCGTCAGAAGGCCGCCATGCAAATACCAGCTGGCCGGCAACCGGCAGGCGGAAATCAGAAACAAC
AATCAGCAGGAGACCAATCA

Product: flavin reductase-like, FMN-binding

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MSANPIPGATLRDDMLMAMRRMARSVAVISCRDGERRYSMSVTAVDSLSADPPSLLICINRNSSIYPPLDAGADFCINLL
AADHRDIAVDCSGRLKGEERFTSGQWDDDMLGVPCLRDAQANLICTQDGRFDYGTHAVFIGRLREVRLAGEVSPLVYVDG
AYTTSAPLASLCPA

Sequences:

>Translated_174_residues
MSANPIPGATLRDDMLMAMRRMARSVAVISCRDGERRYSMSVTAVDSLSADPPSLLICINRNSSIYPPLDAGADFCINLL
AADHRDIAVDCSGRLKGEERFTSGQWDDDMLGVPCLRDAQANLICTQDGRFDYGTHAVFIGRLREVRLAGEVSPLVYVDG
AYTTSAPLASLCPA
>Mature_173_residues
SANPIPGATLRDDMLMAMRRMARSVAVISCRDGERRYSMSVTAVDSLSADPPSLLICINRNSSIYPPLDAGADFCINLLA
ADHRDIAVDCSGRLKGEERFTSGQWDDDMLGVPCLRDAQANLICTQDGRFDYGTHAVFIGRLREVRLAGEVSPLVYVDGA
YTTSAPLASLCPA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=159, Percent_Identity=27.0440251572327, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18832; Mature: 18700

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANPIPGATLRDDMLMAMRRMARSVAVISCRDGERRYSMSVTAVDSLSADPPSLLICIN
CCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEEECCCCCCCCEEEEEEC
RNSSIYPPLDAGADFCINLLAADHRDIAVDCSGRLKGEERFTSGQWDDDMLGVPCLRDAQ
CCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ANLICTQDGRFDYGTHAVFIGRLREVRLAGEVSPLVYVDGAYTTSAPLASLCPA
CCEEEECCCCCCCCCCEEEEEHHHEEEECCCCCCEEEECCCCCCCCCHHHHCCC
>Mature Secondary Structure 
SANPIPGATLRDDMLMAMRRMARSVAVISCRDGERRYSMSVTAVDSLSADPPSLLICIN
CCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEEECCCCCCCCEEEEEEC
RNSSIYPPLDAGADFCINLLAADHRDIAVDCSGRLKGEERFTSGQWDDDMLGVPCLRDAQ
CCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ANLICTQDGRFDYGTHAVFIGRLREVRLAGEVSPLVYVDGAYTTSAPLASLCPA
CCEEEECCCCCCCCCCEEEEEHHHEEEECCCCCCEEEECCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA