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The map label for this gene is kstD [H]

Identifier: 73541233

GI number: 73541233

Start: 1662656

End: 1664410

Strand: Direct

Name: kstD [H]

Synonym: Reut_A1543

Alternate gene names: 73541233

Gene position: 1662656-1664410 (Clockwise)

Preceding gene: 73541232

Following gene: 73541234

Centisome position: 43.68

GC content: 69.17

Gene sequence:

>1755_bases
ATGAGCCAGAGCACAGCGCGCAAGCAGCCGGCGGAATTCGATGTGGTGGTGGTGGGCTCCGGCGCGGGCGGCATGCTGAC
TGCCTGCCGCGCTGCCGACCGCGGCCTGTCGGTGGTGGTACTGGAAAAGAGCAGCCAGTATGGCGGCACGTCGGCCGTGT
CCGGCGGCGGCATCTGGATTCCGCTCAACCACCATATCGCCGCAGCGGGCGCGAAGGACGACTACGCGACCGCACTCGAG
TACATCCTGGCCTGCACGGGCGAACACGGGGATCCGCAACGGCTGCGTGCCTACCTGGACCATGCGCCGCGCATGCTGCA
GTACCTGGAGAAGCAGGCCGGGGTGCACTACTACACGCTGCCGCGCTACGCCGACTACTTCCAGAAGCAACCGGGGGCGA
TGCCGGGCTATCGCTCGCTTGACCCGATGCCGTTCGACGGCGCGCGCCTGCGCGAGGAATTTGACCGCCTGCGCCCGCCG
TCTCCCGGCACGCTGGTGGCCGGCCGCGTGGCGGTCACTTCCGGCGAGGCGCACAAGCTGGTGTGCAAGTCGCCGGGCTG
GCTGTGGTTGGCCGTGCGTCAGTTCGCGCGTTACTGGCTCGACCTTGGCTGGCGCCGCAAGACGCGGCGCGACCGCCGGC
TCACGCTCGGCAACAGCCTGGCTGGCGGCCTGCGGCGCGCGATGATGGACCGGGCCATTCCGCTGTGGCTCGATACGGCG
CTGCAGGACCTGATCATCGAAGACGGCGTGGTCCGTGGCGTTCGCGCGCTGCAGGACGGCCGCGTGGTCGAGATCCGTGC
CTTGCGCGGCGTGGTCCTGGCCGCGGGCGGCTTCGAGCGCAACCAGACCATGCGTGAGCAGTACCTGCCGCAACCGACGC
AGGCCGCGTGGAGCGCTACGCCGCCCAACAATACCGGCGATGCCATCCGCGCCGGCCAGGCCGCCGGTGCCGGCGTCGCA
CTGATGTCCCACATGTGGGGCGCGCCAACCGTGCGCGTGCAGGGCGAGGAGAAGCAGCGCGCGCTCTTTATCGAGCGCGC
CATGCCGGGTTGCATGGTGGTGAACGGGCAGGGGCGGCGCTTCGTCAATGAAGCCGCGCCGTATTCGGAATTCGTGCCTG
CCATGTACCGCGACCACGAGCAGACGGGCTGCAGCGTGCCGGCGTGGATGGTGTTCGACGCGACCTTCCGTCACAAGTAC
CCGTGCGGGCCGATCCTGCCGGGCTCGGTCATGCCGGATCACAGCATCCCCGCCAGCCTGTCCGGCATTCTGGTGAAAGC
CGACACGCTGGATGCGCTGGCCGGGCAGACCGGCATCGATGCCGCGGGGCTGGCCGACAGCGTGGCGCGCATGAACCGCT
ATGCGGCGAGCGGCGTGGACGAGGAGTTCGGCAAGGGCGACAACTTGTTCGATACCTACTACAGCGATCCCACCGTCCGC
CCGAATCCGTGCCTCGCGCCGATTGGCAAGGCGCCGTTCTACGCCGTCCGGCTCGATGCCGGTGACATCGGCACCAAGGG
CGGGCTGGTGACGGACGCCAGCGCCCGCGTGCTGCGCGAAGACGGTTCCGTGATCCCGGGGCTGTTTGCCATCGGCAATA
CCAGCGCCGCGGTGATGGGCACCAGCTATCCAGGCGCGGGCTCCACGCTCGGACCCGCCATGACTTTCGGCTTTATCGCC
GCCGACGTGCTGGCGCAGCAAGCGCGGCAGACTGCCGCGGCGCCCCACACTCAATCCGTTCAGGAGGCCTTATGA

Upstream 100 bases:

>100_bases
CAACCTGGACCGCTTCGGCCAGAACCTCGGCGGCGTGATGATGGGCCGTGCCAACACCGATTTCTTCATCTGAGCCGCGC
TTCGCTCAAGGGAACCGATC

Downstream 100 bases:

>100_bases
GCGCCAATCCCATTCCCGGCGCCACGCTGCGCGACGACATGCTGATGGCCATGCGCCGCATGGCGCGCTCGGTGGCGGTC
ATCAGCTGCCGCGATGGCGA

Product: fumarate reductase/succinate dehydrogenase flavoprotein, N-terminal:FAD dependent oxidoreductase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MSQSTARKQPAEFDVVVVGSGAGGMLTACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWIPLNHHIAAAGAKDDYATALE
YILACTGEHGDPQRLRAYLDHAPRMLQYLEKQAGVHYYTLPRYADYFQKQPGAMPGYRSLDPMPFDGARLREEFDRLRPP
SPGTLVAGRVAVTSGEAHKLVCKSPGWLWLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLAGGLRRAMMDRAIPLWLDTA
LQDLIIEDGVVRGVRALQDGRVVEIRALRGVVLAAGGFERNQTMREQYLPQPTQAAWSATPPNNTGDAIRAGQAAGAGVA
LMSHMWGAPTVRVQGEEKQRALFIERAMPGCMVVNGQGRRFVNEAAPYSEFVPAMYRDHEQTGCSVPAWMVFDATFRHKY
PCGPILPGSVMPDHSIPASLSGILVKADTLDALAGQTGIDAAGLADSVARMNRYAASGVDEEFGKGDNLFDTYYSDPTVR
PNPCLAPIGKAPFYAVRLDAGDIGTKGGLVTDASARVLREDGSVIPGLFAIGNTSAAVMGTSYPGAGSTLGPAMTFGFIA
ADVLAQQARQTAAAPHTQSVQEAL

Sequences:

>Translated_584_residues
MSQSTARKQPAEFDVVVVGSGAGGMLTACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWIPLNHHIAAAGAKDDYATALE
YILACTGEHGDPQRLRAYLDHAPRMLQYLEKQAGVHYYTLPRYADYFQKQPGAMPGYRSLDPMPFDGARLREEFDRLRPP
SPGTLVAGRVAVTSGEAHKLVCKSPGWLWLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLAGGLRRAMMDRAIPLWLDTA
LQDLIIEDGVVRGVRALQDGRVVEIRALRGVVLAAGGFERNQTMREQYLPQPTQAAWSATPPNNTGDAIRAGQAAGAGVA
LMSHMWGAPTVRVQGEEKQRALFIERAMPGCMVVNGQGRRFVNEAAPYSEFVPAMYRDHEQTGCSVPAWMVFDATFRHKY
PCGPILPGSVMPDHSIPASLSGILVKADTLDALAGQTGIDAAGLADSVARMNRYAASGVDEEFGKGDNLFDTYYSDPTVR
PNPCLAPIGKAPFYAVRLDAGDIGTKGGLVTDASARVLREDGSVIPGLFAIGNTSAAVMGTSYPGAGSTLGPAMTFGFIA
ADVLAQQARQTAAAPHTQSVQEAL
>Mature_583_residues
SQSTARKQPAEFDVVVVGSGAGGMLTACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWIPLNHHIAAAGAKDDYATALEY
ILACTGEHGDPQRLRAYLDHAPRMLQYLEKQAGVHYYTLPRYADYFQKQPGAMPGYRSLDPMPFDGARLREEFDRLRPPS
PGTLVAGRVAVTSGEAHKLVCKSPGWLWLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLAGGLRRAMMDRAIPLWLDTAL
QDLIIEDGVVRGVRALQDGRVVEIRALRGVVLAAGGFERNQTMREQYLPQPTQAAWSATPPNNTGDAIRAGQAAGAGVAL
MSHMWGAPTVRVQGEEKQRALFIERAMPGCMVVNGQGRRFVNEAAPYSEFVPAMYRDHEQTGCSVPAWMVFDATFRHKYP
CGPILPGSVMPDHSIPASLSGILVKADTLDALAGQTGIDAAGLADSVARMNRYAASGVDEEFGKGDNLFDTYYSDPTVRP
NPCLAPIGKAPFYAVRLDAGDIGTKGGLVTDASARVLREDGSVIPGLFAIGNTSAAVMGTSYPGAGSTLGPAMTFGFIAA
DVLAQQARQTAAAPHTQSVQEAL

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 62678; Mature: 62547

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTARKQPAEFDVVVVGSGAGGMLTACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWI
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCEEEE
PLNHHIAAAGAKDDYATALEYILACTGEHGDPQRLRAYLDHAPRMLQYLEKQAGVHYYTL
EECCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PRYADYFQKQPGAMPGYRSLDPMPFDGARLREEFDRLRPPSPGTLVAGRVAVTSGEAHKL
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEE
VCKSPGWLWLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLAGGLRRAMMDRAIPLWLDTA
EECCCCEEHHHHHHHHHHHHHCCHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHH
LQDLIIEDGVVRGVRALQDGRVVEIRALRGVVLAAGGFERNQTMREQYLPQPTQAAWSAT
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
PPNNTGDAIRAGQAAGAGVALMSHMWGAPTVRVQGEEKQRALFIERAMPGCMVVNGQGRR
CCCCCCCHHHCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCC
FVNEAAPYSEFVPAMYRDHEQTGCSVPAWMVFDATFRHKYPCGPILPGSVMPDHSIPASL
HHHHCCCHHHHHHHHHHCHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCC
SGILVKADTLDALAGQTGIDAAGLADSVARMNRYAASGVDEEFGKGDNLFDTYYSDPTVR
CCEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHCCCCCCC
PNPCLAPIGKAPFYAVRLDAGDIGTKGGLVTDASARVLREDGSVIPGLFAIGNTSAAVMG
CCCCCCCCCCCCEEEEEECCCCCCCCCCEEECHHHHHHHCCCCCCCCEEEECCCCEEEEE
TSYPGAGSTLGPAMTFGFIAADVLAQQARQTAAAPHTQSVQEAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
SQSTARKQPAEFDVVVVGSGAGGMLTACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWI
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCEEEE
PLNHHIAAAGAKDDYATALEYILACTGEHGDPQRLRAYLDHAPRMLQYLEKQAGVHYYTL
EECCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PRYADYFQKQPGAMPGYRSLDPMPFDGARLREEFDRLRPPSPGTLVAGRVAVTSGEAHKL
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEE
VCKSPGWLWLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLAGGLRRAMMDRAIPLWLDTA
EECCCCEEHHHHHHHHHHHHHCCHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHH
LQDLIIEDGVVRGVRALQDGRVVEIRALRGVVLAAGGFERNQTMREQYLPQPTQAAWSAT
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
PPNNTGDAIRAGQAAGAGVALMSHMWGAPTVRVQGEEKQRALFIERAMPGCMVVNGQGRR
CCCCCCCHHHCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCC
FVNEAAPYSEFVPAMYRDHEQTGCSVPAWMVFDATFRHKYPCGPILPGSVMPDHSIPASL
HHHHCCCHHHHHHHHHHCHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCC
SGILVKADTLDALAGQTGIDAAGLADSVARMNRYAASGVDEEFGKGDNLFDTYYSDPTVR
CCEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHCCCCCCC
PNPCLAPIGKAPFYAVRLDAGDIGTKGGLVTDASARVLREDGSVIPGLFAIGNTSAAVMG
CCCCCCCCCCCCEEEEEECCCCCCCCCCEEECHHHHHHHCCCCCCCCEEEECCCCEEEEE
TSYPGAGSTLGPAMTFGFIAADVLAQQARQTAAAPHTQSVQEAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]