Click here to switch to the map view.

The map label for this gene is bbuR [H]

Identifier: 73541192

GI number: 73541192

Start: 1621318

End: 1622256

Strand: Direct

Name: bbuR [H]

Synonym: Reut_A1501

Alternate gene names: 73541192

Gene position: 1621318-1622256 (Clockwise)

Preceding gene: 73541191

Following gene: 73541204

Centisome position: 42.59

GC content: 66.99

Gene sequence:

>939_bases
ATGACGTCCGCGCTGAATTCCGACGACCTGGCGAGCTTTGTCTGCGTGGCCAACGCGCAAAGCATCTCGCGCGCCGCGCT
GGAACTTGGTGCGGACCAGTCGACGGTAAGCCGCCAGATCGCACGGCTTGAAGCCGCACTCGATACGCGGCTGTTTCATC
GCAGCGGACGCGGCGTGGTGCTGACCGAAGCGGGCGGCACGCTGCTCGGCTACGCGCGGCAAGTGGCGGCCACGCTAACC
GAGGCGCGCGACGCCGTGCGCGCTTCGGCCGCGCAAGGGCCGGCGCAACTGGTCATCGCCGCGCAGCCCACCATTGCCAA
TACCGCTTTTGCGGCCATCGGCACCGCGCTCAAGGCACGTTTCCCGGCGACGAAACTTCGCTTTGTCGAAGGGCTGGCCA
GTCCGGTGCTAGCCTGGCTGGCCGCCGGAGAGATCGACGTGGCCTTGCTCTACTTGCCGGAACAACAGGGCGCACTGAAG
GTGGATGTGCTGCTGGAAGAAGATCTGACGCTGATCACGCCGACATCGTGGAGCCATCTGGGCCCGACGTTCCCGGCGCG
GCGCCTCGGCGAGATTCCGCTGATCCTGCCGAGCACTGCCAACGGCCTGCGCGTGCTGGCCGAATCGCTCGCCACGCGCG
TCGGCACCACGCTGAACATGGCAATGGAATGCGATGCGTCGAATACGGTGTCGATGCGGCTCGTGGAAGACGGATGCGGC
GCAACGTTGCTGCCGTTCGCCGCGGTGGCCGATCGCGTTGCGCAGGGCCGGCTGCATTCGGCGCGCGTGGTGGATCCTGT
CGTCACGCGGCAGGTGGCCCTGACAACGGCGCGCAATCGTGCAGTCGTGCCGGAGTTGTGGGACATCATGCAGACGGTTC
GGCAGTCGGTGCGCAGTATCGTAATGTCGGGGGCGTGGCCGGGGGCGCGGTTGGTTTAG

Upstream 100 bases:

>100_bases
CAGGGCGGGGCTGGCTGGGATCATTCAGGGCTGGTCAAGGCCATCGAGCATCTGTCGAGTTTTGCCATTGGCGATACGCC
GAAAAAGGCCTGATGCCGCG

Downstream 100 bases:

>100_bases
GCAGGAAGTTCACCGCTTCGAGATGCCGGGGTCCAGCCAGGGCGATGCCACGCAAGCGTGGCATCGTGCCCCTTATGGCG
TTCGTTCCGCGCGATCAAAC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTSALNSDDLASFVCVANAQSISRAALELGADQSTVSRQIARLEAALDTRLFHRSGRGVVLTEAGGTLLGYARQVAATLT
EARDAVRASAAQGPAQLVIAAQPTIANTAFAAIGTALKARFPATKLRFVEGLASPVLAWLAAGEIDVALLYLPEQQGALK
VDVLLEEDLTLITPTSWSHLGPTFPARRLGEIPLILPSTANGLRVLAESLATRVGTTLNMAMECDASNTVSMRLVEDGCG
ATLLPFAAVADRVAQGRLHSARVVDPVVTRQVALTTARNRAVVPELWDIMQTVRQSVRSIVMSGAWPGARLV

Sequences:

>Translated_312_residues
MTSALNSDDLASFVCVANAQSISRAALELGADQSTVSRQIARLEAALDTRLFHRSGRGVVLTEAGGTLLGYARQVAATLT
EARDAVRASAAQGPAQLVIAAQPTIANTAFAAIGTALKARFPATKLRFVEGLASPVLAWLAAGEIDVALLYLPEQQGALK
VDVLLEEDLTLITPTSWSHLGPTFPARRLGEIPLILPSTANGLRVLAESLATRVGTTLNMAMECDASNTVSMRLVEDGCG
ATLLPFAAVADRVAQGRLHSARVVDPVVTRQVALTTARNRAVVPELWDIMQTVRQSVRSIVMSGAWPGARLV
>Mature_311_residues
TSALNSDDLASFVCVANAQSISRAALELGADQSTVSRQIARLEAALDTRLFHRSGRGVVLTEAGGTLLGYARQVAATLTE
ARDAVRASAAQGPAQLVIAAQPTIANTAFAAIGTALKARFPATKLRFVEGLASPVLAWLAAGEIDVALLYLPEQQGALKV
DVLLEEDLTLITPTSWSHLGPTFPARRLGEIPLILPSTANGLRVLAESLATRVGTTLNMAMECDASNTVSMRLVEDGCGA
TLLPFAAVADRVAQGRLHSARVVDPVVTRQVALTTARNRAVVPELWDIMQTVRQSVRSIVMSGAWPGARLV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788748, Length=287, Percent_Identity=27.8745644599303, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=276, Percent_Identity=27.1739130434783, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790208, Length=167, Percent_Identity=30.5389221556886, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=174, Percent_Identity=26.4367816091954, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32896; Mature: 32765

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSALNSDDLASFVCVANAQSISRAALELGADQSTVSRQIARLEAALDTRLFHRSGRGVV
CCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTEAGGTLLGYARQVAATLTEARDAVRASAAQGPAQLVIAAQPTIANTAFAAIGTALKAR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHC
FPATKLRFVEGLASPVLAWLAAGEIDVALLYLPEQQGALKVDVLLEEDLTLITPTSWSHL
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEECCCEEECCCCCCCC
GPTFPARRLGEIPLILPSTANGLRVLAESLATRVGTTLNMAMECDASNTVSMRLVEDGCG
CCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCC
ATLLPFAAVADRVAQGRLHSARVVDPVVTRQVALTTARNRAVVPELWDIMQTVRQSVRSI
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VMSGAWPGARLV
HHCCCCCCCCCC
>Mature Secondary Structure 
TSALNSDDLASFVCVANAQSISRAALELGADQSTVSRQIARLEAALDTRLFHRSGRGVV
CCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTEAGGTLLGYARQVAATLTEARDAVRASAAQGPAQLVIAAQPTIANTAFAAIGTALKAR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHC
FPATKLRFVEGLASPVLAWLAAGEIDVALLYLPEQQGALKVDVLLEEDLTLITPTSWSHL
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEECCCEEECCCCCCCC
GPTFPARRLGEIPLILPSTANGLRVLAESLATRVGTTLNMAMECDASNTVSMRLVEDGCG
CCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCC
ATLLPFAAVADRVAQGRLHSARVVDPVVTRQVALTTARNRAVVPELWDIMQTVRQSVRSI
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VMSGAWPGARLV
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9524276; 12910271 [H]