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The map label for this gene is garL [H]

Identifier: 73541190

GI number: 73541190

Start: 1619587

End: 1620387

Strand: Direct

Name: garL [H]

Synonym: Reut_A1499

Alternate gene names: 73541190

Gene position: 1619587-1620387 (Clockwise)

Preceding gene: 73541189

Following gene: 73541191

Centisome position: 42.55

GC content: 65.92

Gene sequence:

>801_bases
ATGACCACTCCCTCCGTTATCCCGCATCTGCCCCAGATCCATCCGCTGCCCAACCGGTTTCGCGCCGACATCCTGGCGCG
CAAGCGCCTGATCGGCTTGTGGAGTTCGCTGGGCCAGCCCGTGACCAGCGAGATCATTGGCCTGGCCGGCTTCGACTGGC
TGCTGCTCGACGGCGAGCATTCGCCCAACGACGTGCTGACACTCGCGCCGCAGCTCATGGCGCTCAAGGACAGCGTCAGC
GCGCCCGTGGTGCGGCCGCCGTGGAACGACCCCGTCATGATCAAGCGCCTGCTCGATATCGGCTTCTATAACTTCCTGAT
CCCGTTCGTGGAATCGGCCGAGGAAGCGCGCCGCGCGGTGCGGGCCACGCGCTATCCGCCTGACGGCATTCGCGGCGTGT
CGGGCGTGCAGCGGCAGAACCGCTTCGGCACGCTGCCGGACTTCTTCAAGACCATCAACGACAATATCTGCGTGCTGGTG
CAGATCGAAAGCCGCAAGGGTGTGGAAGCGGTTGCGGAGATCTGCGCGGAGCCGGGCGTCGATGGCATCTTCATTGGCCC
GAATGACCTGGCTGCAGCCTATGGCCACCTTGGCAACCCGAACCATCCCGATGTGCAGGCGGCCGTGCGGCAGATCTGCG
ATGCGGCCAACCACGCTGGGAAGGCCATCGGCACCCTGACACCCGTGGAGGCCGACGCGCGCCGCTACCTTGACATGGGT
GCGCATTTCGTCGCCGTCGGACTGGATCACGTACTGTTGCGCGATGCGGCGCGCGCGCTGCGCGAGCGTTTCGCGTCCTG
A

Upstream 100 bases:

>100_bases
TTCCCAGCTCGACGATGCCGCCGCGCGCGTGTTGCAGGCACGCGGCAAGTCGCCCGGTCCACTTTCCGCGCTCGCGGCCG
AATGAACAGGAATCCCGACG

Downstream 100 bases:

>100_bases
CACGACTTCAACCGGACACAGACATGAGCAAGATCGGATTCATCGGACTCGGTGTCATGGGCAAGCCCATGGCGGAACAC
CTGCAGGCGGGCGGCCATAT

Product: alpha-dehydro-beta-deoxy-D-glucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTTPSVIPHLPQIHPLPNRFRADILARKRLIGLWSSLGQPVTSEIIGLAGFDWLLLDGEHSPNDVLTLAPQLMALKDSVS
APVVRPPWNDPVMIKRLLDIGFYNFLIPFVESAEEARRAVRATRYPPDGIRGVSGVQRQNRFGTLPDFFKTINDNICVLV
QIESRKGVEAVAEICAEPGVDGIFIGPNDLAAAYGHLGNPNHPDVQAAVRQICDAANHAGKAIGTLTPVEADARRYLDMG
AHFVAVGLDHVLLRDAARALRERFAS

Sequences:

>Translated_266_residues
MTTPSVIPHLPQIHPLPNRFRADILARKRLIGLWSSLGQPVTSEIIGLAGFDWLLLDGEHSPNDVLTLAPQLMALKDSVS
APVVRPPWNDPVMIKRLLDIGFYNFLIPFVESAEEARRAVRATRYPPDGIRGVSGVQRQNRFGTLPDFFKTINDNICVLV
QIESRKGVEAVAEICAEPGVDGIFIGPNDLAAAYGHLGNPNHPDVQAAVRQICDAANHAGKAIGTLTPVEADARRYLDMG
AHFVAVGLDHVLLRDAARALRERFAS
>Mature_265_residues
TTPSVIPHLPQIHPLPNRFRADILARKRLIGLWSSLGQPVTSEIIGLAGFDWLLLDGEHSPNDVLTLAPQLMALKDSVSA
PVVRPPWNDPVMIKRLLDIGFYNFLIPFVESAEEARRAVRATRYPPDGIRGVSGVQRQNRFGTLPDFFKTINDNICVLVQ
IESRKGVEAVAEICAEPGVDGIFIGPNDLAAAYGHLGNPNHPDVQAAVRQICDAANHAGKAIGTLTPVEADARRYLDMGA
HFVAVGLDHVLLRDAARALRERFAS

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=61.8473895582329, Blast_Score=320, Evalue=5e-89,
Organism=Escherichia coli, GI1788578, Length=251, Percent_Identity=42.6294820717131, Blast_Score=195, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 29055; Mature: 28924

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSVIPHLPQIHPLPNRFRADILARKRLIGLWSSLGQPVTSEIIGLAGFDWLLLDGEH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCC
SPNDVLTLAPQLMALKDSVSAPVVRPPWNDPVMIKRLLDIGFYNFLIPFVESAEEARRAV
CCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RATRYPPDGIRGVSGVQRQNRFGTLPDFFKTINDNICVLVQIESRKGVEAVAEICAEPGV
HHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCC
DGIFIGPNDLAAAYGHLGNPNHPDVQAAVRQICDAANHAGKAIGTLTPVEADARRYLDMG
CEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHC
AHFVAVGLDHVLLRDAARALRERFAS
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPSVIPHLPQIHPLPNRFRADILARKRLIGLWSSLGQPVTSEIIGLAGFDWLLLDGEH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCC
SPNDVLTLAPQLMALKDSVSAPVVRPPWNDPVMIKRLLDIGFYNFLIPFVESAEEARRAV
CCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RATRYPPDGIRGVSGVQRQNRFGTLPDFFKTINDNICVLVQIESRKGVEAVAEICAEPGV
HHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCC
DGIFIGPNDLAAAYGHLGNPNHPDVQAAVRQICDAANHAGKAIGTLTPVEADARRYLDMG
CEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHC
AHFVAVGLDHVLLRDAARALRERFAS
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA