Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 73540974

GI number: 73540974

Start: 1365554

End: 1368001

Strand: Direct

Name: pheT

Synonym: Reut_A1280

Alternate gene names: 73540974

Gene position: 1365554-1368001 (Clockwise)

Preceding gene: 161611263

Following gene: 73540975

Centisome position: 35.87

GC content: 64.71

Gene sequence:

>2448_bases
ATGCAATTCTCGGAATCCTGGCTTCGCACCTTCGCCAACCCTGAAAAGATCTCCACCGACGCGCTGTCGCACAGCCTGAC
CATGGCCGGGCTCGAAGTGGAGGAGGTCGGCCCGGTCGCGCCGCCGTTCGACAAGATCGTGGTCGCGCACGTGCTGTCGA
CTGAGCGCCATCCCAATGCAGACCGGCTCAACGTGTGCCAGGTCGACGCCGGCACCGGCGAGACGCTGCAGATCGTCTGC
GGTGCACCCAACGTCAAGCCCGGCATCAAGGTCCCGTGCGCGCTGGTGGGCGCCGTGCTGCCGCCTGCAGAGGCCGAAGG
CAAGCCGTTCGAAATCAAGGTCGGCAAGCTGCGTGGCGTGGAGAGCTACGGCATGCTTTGCTCGGCGCGCGAGCTGAAGC
TGTCGGAAGACCATGGCGGCCTGCTGGTGCTGCCGGAAGATGCGCCGGTCGGCCAGAACATCCGCGAATACCTGGACCTC
GACGATCAGGTCTTCATCATCAAGCTGACGCCGAACAAGGCTGACTGCCTGTCGATCCACGGTGTGGCGCGTGAAGTGTC
GGCCCTGACCGGCGCTACGCTGACGCTGCCGGACATGAAGCCCGTGGCGGTCACCATCGACGACAAGCTGCCGGTAAAGG
TGTCGGCGCCCGACCTGTGCGGCCGCTTCTCGGGTCGCGTGATCCGCGGCGTGAACGCACGCGCGGCTACGCCGGCCTGG
ATGGTGCAGCGCCTGGAGCGCTCGGGCCAGCGCAGCATCTCGGCGATGGTGGATATCTCCAACTACGTGATGCTCGAGCT
GGGCCGCCCGTCGCACGTGTTCGACCTGGACAAAATCCACGGCGGCCTCGATGTGCGTTGGGGCCGCAAGGGCGAACAGA
TCAAGCTGCTGAACGGCAATACCATCGAGGTGGACGAGCAAGTCGGCGTGATCGCTGACGACAAGGAAATCGAGAGCCTG
GCCGGCATCATGGGTGGCGACAGCACTGCCGTGACGCTCGACACGACGAACATCTACCTGGAAGCCGCGTTCTGGTGGCC
GTCGGCCATCCAGGGCCGCGCGCGCCGCTACAACTTCTCGACCGATGCGGCGCATCGCTTCGAGCGCGGCGTGGACTATG
CGACGACGGTTGAGCATATCGAACGCATCACCGCGCTGATCCTCGAGATCTGCGGTGGCCAGGCCGGTCCGGTCGATGAC
CATATCGTCAATCTTCCGCAGCGTAGACCGGTCAGCCTGCGTCTGGCGCGTGCCGAGCGCGTGCTGGGCATCGAGCTGTC
GTCGGCCGTCGTGGCTGATGTATTCCAGCGCTTGCAACTGCCGTTCACGCGCTCGCAGGGCGCCGACGGCGAAGTGTTCG
AGGTGACCCCGCCGAGCTATCGCTTCGACATCGAGATCGAAGAGGACCTGATCGAAGAAGTCGCGCGCATCTACGGCTTC
GAGCGCATTGCCGCGCGTCCGCCCGTGGCGGAGAGCGAAATGCGCCCGACCAACGAAGGCCGCCGCTCCACGCACGTAGT
GCGCCACGCGCTGGCCGCGCGCGACTACCAGGAAGTCATCAACTTTGCCTTCGTGGAAGAGAAGTGGGAGCGCGACTTCG
CCGCCAATGACAACCCGATCCGCTTGCTGAATCCCATCGCGAGCCAGCTTGCGGTAATGCGTTCCTCGCTGATCGGTGGC
CTGCTCGACAAGGTGCGCTACAACCTGAATCGCAAGGCCGCGCGCGTGCGCCTGTTCGAAGTCGGCCGCGTGTTCCATCG
CGATGCCGATGTGAAGGACGGCGGCCTGACGGTGGCCGGCTACCATCAACCGATGATGGCTGCCGGTATCGCCTACGGTC
CCGCATTCGAAGAGCAATGGGGCATCACCACGCGCAACGTCGATTTCTTCGACGTGAAGGGCGATGTGGAAACGCTGTTC
TATCCGCGTGTTGCTCGCTTCGAACCGGTCGAACATCCGGCTTTGCACCCGGGCCGCGCGGCACGCATGCTGATCGACGG
CAAGCCGGTCGGCGTCGTGGGCGAAATGCATCCGCGCTGGCTGCAGGAGTATGAACTGACGCAGGCGCCGGTACTGTTCG
AACTCGAGCTTGACGCACTGCGCGAAGCAGGTCTGCCGACCTACGCCGAGATCTCGAAGTTCCCTGCCGCCGTGCGTGAT
CTGGCGGTAGTGGTGAAGCAATCGGTACGCGTGCAGGACATGCTTGACAGCATGCGCGCCGCGCTCGACAAGCAGGGCTG
TGGCCGCTTCTGCCAGAGCCTCGTGCTGTTCGACGAGTTCCGTCCGAAGGCTGCTTCGGCCGCCATTGGGGCCGACGAGA
AAAGCCTTGCGTTCCGGGTGACCTTGCAAGATACTGGGTCGACCCTGCAGGACGAGACCGTCGACAGCGCGGTGCGCTGC
ATGGTCGACGCATTGGGCGAAGCCTTCCAGGCTCGCTTGCGCGGCTGA

Upstream 100 bases:

>100_bases
TCTTCGAAGGCGACGTGCGCTTCCTGCGCCAGTTCGCCTGACGCGCGCGTCCAGCCCTTCGTCAAAGACATCACAGAATA
CTTACCGGATCAGAAGCGCC

Downstream 100 bases:

>100_bases
CCACCAGCACATACCACAGACAGGGCGCCCGGGCAGTGCACCCGGGCGGATCGCCAGACAGAATTCCTTGAGCGATCGAC
ATGAAAGATCGAGACGCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MQFSESWLRTFANPEKISTDALSHSLTMAGLEVEEVGPVAPPFDKIVVAHVLSTERHPNADRLNVCQVDAGTGETLQIVC
GAPNVKPGIKVPCALVGAVLPPAEAEGKPFEIKVGKLRGVESYGMLCSARELKLSEDHGGLLVLPEDAPVGQNIREYLDL
DDQVFIIKLTPNKADCLSIHGVAREVSALTGATLTLPDMKPVAVTIDDKLPVKVSAPDLCGRFSGRVIRGVNARAATPAW
MVQRLERSGQRSISAMVDISNYVMLELGRPSHVFDLDKIHGGLDVRWGRKGEQIKLLNGNTIEVDEQVGVIADDKEIESL
AGIMGGDSTAVTLDTTNIYLEAAFWWPSAIQGRARRYNFSTDAAHRFERGVDYATTVEHIERITALILEICGGQAGPVDD
HIVNLPQRRPVSLRLARAERVLGIELSSAVVADVFQRLQLPFTRSQGADGEVFEVTPPSYRFDIEIEEDLIEEVARIYGF
ERIAARPPVAESEMRPTNEGRRSTHVVRHALAARDYQEVINFAFVEEKWERDFAANDNPIRLLNPIASQLAVMRSSLIGG
LLDKVRYNLNRKAARVRLFEVGRVFHRDADVKDGGLTVAGYHQPMMAAGIAYGPAFEEQWGITTRNVDFFDVKGDVETLF
YPRVARFEPVEHPALHPGRAARMLIDGKPVGVVGEMHPRWLQEYELTQAPVLFELELDALREAGLPTYAEISKFPAAVRD
LAVVVKQSVRVQDMLDSMRAALDKQGCGRFCQSLVLFDEFRPKAASAAIGADEKSLAFRVTLQDTGSTLQDETVDSAVRC
MVDALGEAFQARLRG

Sequences:

>Translated_815_residues
MQFSESWLRTFANPEKISTDALSHSLTMAGLEVEEVGPVAPPFDKIVVAHVLSTERHPNADRLNVCQVDAGTGETLQIVC
GAPNVKPGIKVPCALVGAVLPPAEAEGKPFEIKVGKLRGVESYGMLCSARELKLSEDHGGLLVLPEDAPVGQNIREYLDL
DDQVFIIKLTPNKADCLSIHGVAREVSALTGATLTLPDMKPVAVTIDDKLPVKVSAPDLCGRFSGRVIRGVNARAATPAW
MVQRLERSGQRSISAMVDISNYVMLELGRPSHVFDLDKIHGGLDVRWGRKGEQIKLLNGNTIEVDEQVGVIADDKEIESL
AGIMGGDSTAVTLDTTNIYLEAAFWWPSAIQGRARRYNFSTDAAHRFERGVDYATTVEHIERITALILEICGGQAGPVDD
HIVNLPQRRPVSLRLARAERVLGIELSSAVVADVFQRLQLPFTRSQGADGEVFEVTPPSYRFDIEIEEDLIEEVARIYGF
ERIAARPPVAESEMRPTNEGRRSTHVVRHALAARDYQEVINFAFVEEKWERDFAANDNPIRLLNPIASQLAVMRSSLIGG
LLDKVRYNLNRKAARVRLFEVGRVFHRDADVKDGGLTVAGYHQPMMAAGIAYGPAFEEQWGITTRNVDFFDVKGDVETLF
YPRVARFEPVEHPALHPGRAARMLIDGKPVGVVGEMHPRWLQEYELTQAPVLFELELDALREAGLPTYAEISKFPAAVRD
LAVVVKQSVRVQDMLDSMRAALDKQGCGRFCQSLVLFDEFRPKAASAAIGADEKSLAFRVTLQDTGSTLQDETVDSAVRC
MVDALGEAFQARLRG
>Mature_815_residues
MQFSESWLRTFANPEKISTDALSHSLTMAGLEVEEVGPVAPPFDKIVVAHVLSTERHPNADRLNVCQVDAGTGETLQIVC
GAPNVKPGIKVPCALVGAVLPPAEAEGKPFEIKVGKLRGVESYGMLCSARELKLSEDHGGLLVLPEDAPVGQNIREYLDL
DDQVFIIKLTPNKADCLSIHGVAREVSALTGATLTLPDMKPVAVTIDDKLPVKVSAPDLCGRFSGRVIRGVNARAATPAW
MVQRLERSGQRSISAMVDISNYVMLELGRPSHVFDLDKIHGGLDVRWGRKGEQIKLLNGNTIEVDEQVGVIADDKEIESL
AGIMGGDSTAVTLDTTNIYLEAAFWWPSAIQGRARRYNFSTDAAHRFERGVDYATTVEHIERITALILEICGGQAGPVDD
HIVNLPQRRPVSLRLARAERVLGIELSSAVVADVFQRLQLPFTRSQGADGEVFEVTPPSYRFDIEIEEDLIEEVARIYGF
ERIAARPPVAESEMRPTNEGRRSTHVVRHALAARDYQEVINFAFVEEKWERDFAANDNPIRLLNPIASQLAVMRSSLIGG
LLDKVRYNLNRKAARVRLFEVGRVFHRDADVKDGGLTVAGYHQPMMAAGIAYGPAFEEQWGITTRNVDFFDVKGDVETLF
YPRVARFEPVEHPALHPGRAARMLIDGKPVGVVGEMHPRWLQEYELTQAPVLFELELDALREAGLPTYAEISKFPAAVRD
LAVVVKQSVRVQDMLDSMRAALDKQGCGRFCQSLVLFDEFRPKAASAAIGADEKSLAFRVTLQDTGSTLQDETVDSAVRC
MVDALGEAFQARLRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=387, Percent_Identity=24.2894056847545, Blast_Score=91, Evalue=7e-18,
Organism=Escherichia coli, GI1788006, Length=822, Percent_Identity=43.4306569343066, Blast_Score=635, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=391, Percent_Identity=24.8081841432225, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_CUPPJ (Q472N3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295494.1
- ProteinModelPortal:   Q472N3
- SMR:   Q472N3
- GeneID:   3609271
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1280
- NMPDR:   fig|264198.3.peg.1926
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   REUT264198:REUT_A1280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 89707; Mature: 89707

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSESWLRTFANPEKISTDALSHSLTMAGLEVEEVGPVAPPFDKIVVAHVLSTERHPNA
CCCCHHHHHHCCCCHHCCHHHHHHHHHEECCCHHHCCCCCCCHHHHHHHHHHHCCCCCCC
DRLNVCQVDAGTGETLQIVCGAPNVKPGIKVPCALVGAVLPPAEAEGKPFEIKVGKLRGV
CCCEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCC
ESYGMLCSARELKLSEDHGGLLVLPEDAPVGQNIREYLDLDDQVFIIKLTPNKADCLSIH
HHHCCEECCCCCEEECCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCEEEHH
GVAREVSALTGATLTLPDMKPVAVTIDDKLPVKVSAPDLCGRFSGRVIRGVNARAATPAW
HHHHHHHHHCCCEEECCCCCCEEEEECCCCCEEECCCHHHHHCCCCEEECCCCCCCCHHH
MVQRLERSGQRSISAMVDISNYVMLELGRPSHVFDLDKIHGGLDVRWGRKGEQIKLLNGN
HHHHHHHCCHHHHHHEEEECCEEEEEECCCCCEEEHHHHCCCEEEEECCCCCEEEEECCC
TIEVDEQVGVIADDKEIESLAGIMGGDSTAVTLDTTNIYLEAAFWWPSAIQGRARRYNFS
EEEECCCCCEEECCHHHHHHHHHCCCCCEEEEEEECEEEEEEEECCCHHHCCCHHCCCCC
TDAAHRFERGVDYATTVEHIERITALILEICGGQAGPVDDHIVNLPQRRPVSLRLARAER
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEEEEHHHH
VLGIELSSAVVADVFQRLQLPFTRSQGADGEVFEVTPPSYRFDIEIEEDLIEEVARIYGF
EEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEEEHHHHHHHHHHHHCH
ERIAARPPVAESEMRPTNEGRRSTHVVRHALAARDYQEVINFAFVEEKWERDFAANDNPI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
RLLNPIASQLAVMRSSLIGGLLDKVRYNLNRKAARVRLFEVGRVFHRDADVKDGGLTVAG
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
YHQPMMAAGIAYGPAFEEQWGITTRNVDFFDVKGDVETLFYPRVARFEPVEHPALHPGRA
CCCHHHHHHCCCCCCCHHHCCCEECCCEEEEECCCHHHHCCCCHHCCCCCCCCCCCCCCC
ARMLIDGKPVGVVGEMHPRWLQEYELTQAPVLFELELDALREAGLPTYAEISKFPAAVRD
EEEEECCCCCEEECCCCHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCHHHHHHHHHHHHH
LAVVVKQSVRVQDMLDSMRAALDKQGCGRFCQSLVLFDEFRPKAASAAIGADEKSLAFRV
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEE
TLQDTGSTLQDETVDSAVRCMVDALGEAFQARLRG
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQFSESWLRTFANPEKISTDALSHSLTMAGLEVEEVGPVAPPFDKIVVAHVLSTERHPNA
CCCCHHHHHHCCCCHHCCHHHHHHHHHEECCCHHHCCCCCCCHHHHHHHHHHHCCCCCCC
DRLNVCQVDAGTGETLQIVCGAPNVKPGIKVPCALVGAVLPPAEAEGKPFEIKVGKLRGV
CCCEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCC
ESYGMLCSARELKLSEDHGGLLVLPEDAPVGQNIREYLDLDDQVFIIKLTPNKADCLSIH
HHHCCEECCCCCEEECCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCEEEHH
GVAREVSALTGATLTLPDMKPVAVTIDDKLPVKVSAPDLCGRFSGRVIRGVNARAATPAW
HHHHHHHHHCCCEEECCCCCCEEEEECCCCCEEECCCHHHHHCCCCEEECCCCCCCCHHH
MVQRLERSGQRSISAMVDISNYVMLELGRPSHVFDLDKIHGGLDVRWGRKGEQIKLLNGN
HHHHHHHCCHHHHHHEEEECCEEEEEECCCCCEEEHHHHCCCEEEEECCCCCEEEEECCC
TIEVDEQVGVIADDKEIESLAGIMGGDSTAVTLDTTNIYLEAAFWWPSAIQGRARRYNFS
EEEECCCCCEEECCHHHHHHHHHCCCCCEEEEEEECEEEEEEEECCCHHHCCCHHCCCCC
TDAAHRFERGVDYATTVEHIERITALILEICGGQAGPVDDHIVNLPQRRPVSLRLARAER
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEEEEHHHH
VLGIELSSAVVADVFQRLQLPFTRSQGADGEVFEVTPPSYRFDIEIEEDLIEEVARIYGF
EEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEEEHHHHHHHHHHHHCH
ERIAARPPVAESEMRPTNEGRRSTHVVRHALAARDYQEVINFAFVEEKWERDFAANDNPI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
RLLNPIASQLAVMRSSLIGGLLDKVRYNLNRKAARVRLFEVGRVFHRDADVKDGGLTVAG
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
YHQPMMAAGIAYGPAFEEQWGITTRNVDFFDVKGDVETLFYPRVARFEPVEHPALHPGRA
CCCHHHHHHCCCCCCCHHHCCCEECCCEEEEECCCHHHHCCCCHHCCCCCCCCCCCCCCC
ARMLIDGKPVGVVGEMHPRWLQEYELTQAPVLFELELDALREAGLPTYAEISKFPAAVRD
EEEEECCCCCEEECCCCHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCHHHHHHHHHHHHH
LAVVVKQSVRVQDMLDSMRAALDKQGCGRFCQSLVLFDEFRPKAASAAIGADEKSLAFRV
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEE
TLQDTGSTLQDETVDSAVRCMVDALGEAFQARLRG
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA