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The map label for this gene is pheS

Identifier: 161611263

GI number: 161611263

Start: 1364463

End: 1365494

Strand: Direct

Name: pheS

Synonym: Reut_A1279

Alternate gene names: 161611263

Gene position: 1364463-1365494 (Clockwise)

Preceding gene: 73540972

Following gene: 73540974

Centisome position: 35.85

GC content: 65.21

Gene sequence:

>1032_bases
ATGTCCCAGGATCTGGATCAAATCGTCGCCGACGCCCAGGCCGCTTTCGCCGCCGCCAACGACAACGCCACGCTGGAAAA
CGAGAAGGCCAGGTTCCTCGGCAAGACCGGCGCGCTGACCGAACTGCTCAAGGGCCTCGGCAAGCTCGACCCGGAAACGC
GCAAGACTGAAGGCGCGCGCATCAACCAGGTCAAGCAGCAGGTCGAAGCCGCGCTGACGGCGCGCCGCCAGGCACTGGCC
GATGCGCTGATGAATGCGCGCCTGTCTGCCGAGGCCATCGACGTGACCCTGCCGGGCCGCGCGGTCAGCCGCGGCAGCCT
GCACCCGGTCATGCGCACGTGGGAACGCGTGGAACAGATCTTCGGCTCGATCGGCTTCGACGTGGCCGATGGTCCCGAGA
TCGAAACCGACTGGATGAACTTCACCGCGCTCAACAACCCGGACAACCATCCGGCGCGTTCGATGCAGGACACGTTCTAT
ATCGACGGCCGCGACAGCGACGACAAGCTCCTGCTGCTGCGCACGCACACCAGCCCCATGCAGGTGCGCTACGCGAAGAT
GCACGTCGAGAAGTACGCCGGCAAGGCCATGCCGCCGATCAAGGTGATCTGCCCGGGCCGCACGTACCGCGTCGACAGCG
ACGCCACGCACTCGCCGATGTTCAACCAGGTCGAAGGCCTGTGGATCGGCGAGGACGTGAGCTTTGCGGACCTGAAGGGT
GTGTACACCGACTTCCTGCGCAAGTTCTTCGAGCGCGACGACATCCAGGTGCGTTTCCGCCCGTCGTACTTCCCGTTCAC
GGAACCGTCGGCCGAGATCGACATGGCGTTCGGCAACGGCAAGTGGCTGGAGATCTCCGGCTCCGGCCAGGTGCACCCGA
ACGTGCTGCGCAATATGGGCCTGGACCCGGAACGCTACATCGGCTTCGCGTTCGGCTCGGGCCTGGAGCGCCTGACGATG
CTGCGCTATGGCATCAACGACCTGCGCCTGTTCTTCGAAGGCGACGTGCGCTTCCTGCGCCAGTTCGCCTGA

Upstream 100 bases:

>100_bases
GTCCTTGCCGCAAGCAGTGCCGAAACGCCATTCGAAACGAACACCAACCCACGCCGCGACGGCAGCAATGTCACGGCGCC
AAGCTATTTCCACGACCGCC

Downstream 100 bases:

>100_bases
CGCGCGCGTCCAGCCCTTCGTCAAAGACATCACAGAATACTTACCGGATCAGAAGCGCCATGCAATTCTCGGAATCCTGG
CTTCGCACCTTCGCCAACCC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSQDLDQIVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPETRKTEGARINQVKQQVEAALTARRQALA
DALMNARLSAEAIDVTLPGRAVSRGSLHPVMRTWERVEQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFY
IDGRDSDDKLLLLRTHTSPMQVRYAKMHVEKYAGKAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGEDVSFADLKG
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMGLDPERYIGFAFGSGLERLTM
LRYGINDLRLFFEGDVRFLRQFA

Sequences:

>Translated_343_residues
MSQDLDQIVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPETRKTEGARINQVKQQVEAALTARRQALA
DALMNARLSAEAIDVTLPGRAVSRGSLHPVMRTWERVEQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFY
IDGRDSDDKLLLLRTHTSPMQVRYAKMHVEKYAGKAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGEDVSFADLKG
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMGLDPERYIGFAFGSGLERLTM
LRYGINDLRLFFEGDVRFLRQFA
>Mature_342_residues
SQDLDQIVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPETRKTEGARINQVKQQVEAALTARRQALAD
ALMNARLSAEAIDVTLPGRAVSRGSLHPVMRTWERVEQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYI
DGRDSDDKLLLLRTHTSPMQVRYAKMHVEKYAGKAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGEDVSFADLKGV
YTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMGLDPERYIGFAFGSGLERLTML
RYGINDLRLFFEGDVRFLRQFA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=264, Percent_Identity=34.469696969697, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI5729820, Length=294, Percent_Identity=30.2721088435374, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=56.2130177514793, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=34.3173431734317, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=34.3173431734317, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566635, Length=253, Percent_Identity=31.2252964426877, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=364, Percent_Identity=29.9450549450549, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=44.4444444444444, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18858079, Length=299, Percent_Identity=31.438127090301, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=252, Percent_Identity=29.3650793650794, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CUPPJ (Q472N4)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295493.2
- GeneID:   3609270
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1279
- NMPDR:   fig|264198.3.peg.1925
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   REUT264198:REUT_A1279-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38566; Mature: 38435

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDLDQIVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPETRKTEGAR
CCCHHHHHHHHHHHHHEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCHHCCCCHH
INQVKQQVEAALTARRQALADALMNARLSAEAIDVTLPGRAVSRGSLHPVMRTWERVEQI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYIDGRDSDDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYAKMHVEKYAGKAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGEDVSFADLKG
HHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCHHHCCCCEEECCCCCHHHHHH
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMG
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCC
LDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEECCCHHHHHHCC
>Mature Secondary Structure 
SQDLDQIVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPETRKTEGAR
CCHHHHHHHHHHHHHEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCHHCCCCHH
INQVKQQVEAALTARRQALADALMNARLSAEAIDVTLPGRAVSRGSLHPVMRTWERVEQI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYIDGRDSDDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYAKMHVEKYAGKAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGEDVSFADLKG
HHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCHHHCCCCEEECCCCCHHHHHH
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMG
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCC
LDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA