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The map label for this gene is pykA [H]

Identifier: 73540264

GI number: 73540264

Start: 603986

End: 605422

Strand: Reverse

Name: pykA [H]

Synonym: Reut_A0558

Alternate gene names: 73540264

Gene position: 605422-603986 (Counterclockwise)

Preceding gene: 73540265

Following gene: 73540263

Centisome position: 15.9

GC content: 66.67

Gene sequence:

>1437_bases
ATGACCCGCTCCACCAAGATCGTCGCCACCATCGGCCCCGCTTCCAGCTCGCTGGAAGTGCTGACGCGCATGATTGCCGC
GGGGGTCGACGTGGTGCGGCTGAATTTCTCGCACGGCACCGCGCAGGACCATATCGACCGCGCCACGCTGGTGCGCGAAG
CCGCGCAGGCCTGCGGGCGCGAAGTGGCCATCATGGCCGACCTGCAGGGCCCGAAGATCCGCGTGGGCAAGTTCGAGAGC
GGCAAGGTCACGCTCAAGCCGGGCGACCCGTTCGTGCTCGACTCGTCGTGCCAGCTCGGCAACCAGGAACGCGTCGGCCT
GGACTACCAGGACCTGCCGCGCGACGTGGGCCCAGGCGACCTGCTGCTGCTCAACGACGGCCTGATCGTGCTGGTCGTGG
ACCGTGTGCTCGGCACGGAGATCTTCACCACCGTGCGCATCGGCGGCGACCTGTCGAACAACAAGGGTATCAACCGCCAG
GGCGGCGGGCTGTCGGCGCCGGCGCTGACGGCCAAGGACATGGACGACATCAAGACCGCCATGGCCCTGGGCGCGGACTA
TGTCGCGGTGAGCTTCCCGAAGAACGCCACCGATATGGAAATGGCGCGCCAGCTCGCAGCGGTCGCAGGCCAGCCGTATG
GCCGCAAGGCGCGCATGATCGCCAAGATCGAGCGCGCCGAAGCGATCAACCCGGGCGTGCTTGAAGAAATCCTGCAGGCG
TCTGACGGCATCATGGTCGCGCGCGGCGACCTGGCCGTGGAAGTCGGCAATGCCGCGGTGCCGGCACTGCAGAAGCGCAT
GATCAAGCTCGCGCGCGAGGCCAACAAGCTGACCATCACCGCCACGCAGATGATGGAAAGCATGATCGTGAACCCGGTGC
CGACGCGCGCCGAGGTGTCCGACGTGGCCAACGCCGTGCTCGACGGCACCGACGCCGTCATGCTGTCGGCCGAGACCGCC
GCCGGGCGCTATCCGGTCGAGACGGTCGAGGCCATGGCCGCAGTCTGCATCGAAGCCGAGAAGTCCGAAGTCGTGCAGCT
TGACACGGACTTCCTCAATCAGACCTTTTCGCGCATCGACCAGTCAGTGGCAATGGGTGCGCTGTTCACCGCCTATCATT
TGCAGGTGAAGGCGATTGCCGCACTGACCGATTCGGGCGCGACTGCGCTGTGGATGAGCCGTCACCGCATCCACGTGCCG
ATCTACGCGATGACGCCCAACGTGGCGTCGCAGCGCAAGATGCAGCTGTACCGCAACGTCGTGCCGCTGCCGCTTGACGC
CAGCACCGACCGCGACACCGCGCTGGAGCAGGCCGAAGAGCTGCTGCTCGCGCAGGGCGTGGTGCAGCGCGGCGATTTCA
TCGTGCTGACCATCGGCGAGCCCATGGGCCAGCCCGGCGGCACCAACACCCTCAAGATCGTCAGGGTGGGAAGTTGA

Upstream 100 bases:

>100_bases
AGTTCCTGGAAGGCAAGAAGCTGCCCGCATTCGAAGTGCTGGAGCAACGCGCAGCAGGCTGAGGCCGCGCTGGCCCGCGT
CCGGACAAGGATCCCCCGCC

Downstream 100 bases:

>100_bases
AGCCATTTGTGCGGCGGCGGGCAGAAGCACTACGGCAAGCGGTGCAAAAGGCGCTGCGCCGCAGGCTTGGCCCGCGCCAG
CAGAACAAGATTCAGAGACA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTRSTKIVATIGPASSSLEVLTRMIAAGVDVVRLNFSHGTAQDHIDRATLVREAAQACGREVAIMADLQGPKIRVGKFES
GKVTLKPGDPFVLDSSCQLGNQERVGLDYQDLPRDVGPGDLLLLNDGLIVLVVDRVLGTEIFTTVRIGGDLSNNKGINRQ
GGGLSAPALTAKDMDDIKTAMALGADYVAVSFPKNATDMEMARQLAAVAGQPYGRKARMIAKIERAEAINPGVLEEILQA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETA
AGRYPVETVEAMAAVCIEAEKSEVVQLDTDFLNQTFSRIDQSVAMGALFTAYHLQVKAIAALTDSGATALWMSRHRIHVP
IYAMTPNVASQRKMQLYRNVVPLPLDASTDRDTALEQAEELLLAQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGS

Sequences:

>Translated_478_residues
MTRSTKIVATIGPASSSLEVLTRMIAAGVDVVRLNFSHGTAQDHIDRATLVREAAQACGREVAIMADLQGPKIRVGKFES
GKVTLKPGDPFVLDSSCQLGNQERVGLDYQDLPRDVGPGDLLLLNDGLIVLVVDRVLGTEIFTTVRIGGDLSNNKGINRQ
GGGLSAPALTAKDMDDIKTAMALGADYVAVSFPKNATDMEMARQLAAVAGQPYGRKARMIAKIERAEAINPGVLEEILQA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETA
AGRYPVETVEAMAAVCIEAEKSEVVQLDTDFLNQTFSRIDQSVAMGALFTAYHLQVKAIAALTDSGATALWMSRHRIHVP
IYAMTPNVASQRKMQLYRNVVPLPLDASTDRDTALEQAEELLLAQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGS
>Mature_477_residues
TRSTKIVATIGPASSSLEVLTRMIAAGVDVVRLNFSHGTAQDHIDRATLVREAAQACGREVAIMADLQGPKIRVGKFESG
KVTLKPGDPFVLDSSCQLGNQERVGLDYQDLPRDVGPGDLLLLNDGLIVLVVDRVLGTEIFTTVRIGGDLSNNKGINRQG
GGLSAPALTAKDMDDIKTAMALGADYVAVSFPKNATDMEMARQLAAVAGQPYGRKARMIAKIERAEAINPGVLEEILQAS
DGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETAA
GRYPVETVEAMAAVCIEAEKSEVVQLDTDFLNQTFSRIDQSVAMGALFTAYHLQVKAIAALTDSGATALWMSRHRIHVPI
YAMTPNVASQRKMQLYRNVVPLPLDASTDRDTALEQAEELLLAQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=495, Percent_Identity=37.979797979798, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI33286420, Length=495, Percent_Identity=37.979797979798, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=38.3838383838384, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=38.1818181818182, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=38.1818181818182, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=38.013698630137, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=38.013698630137, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=35.5648535564854, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=35.5648535564854, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=33.4862385321101, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=51.8828451882845, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1787965, Length=478, Percent_Identity=39.9581589958159, Blast_Score=307, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=494, Percent_Identity=37.246963562753, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=495, Percent_Identity=36.969696969697, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=495, Percent_Identity=37.1717171717172, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=495, Percent_Identity=36.969696969697, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=495, Percent_Identity=36.969696969697, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=495, Percent_Identity=35.5555555555556, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=495, Percent_Identity=33.7373737373737, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=38.2591093117409, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=38.2591093117409, Blast_Score=279, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24648966, Length=419, Percent_Identity=36.0381861575179, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=32.4489795918367, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24646914, Length=281, Percent_Identity=36.2989323843416, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50957; Mature: 50826

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSTKIVATIGPASSSLEVLTRMIAAGVDVVRLNFSHGTAQDHIDRATLVREAAQACGR
CCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFESGKVTLKPGDPFVLDSSCQLGNQERVGLDYQDLPRDVGPGD
EEEEEEECCCCEEEEEEECCCEEEECCCCCEEECCCCCCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLVVDRVLGTEIFTTVRIGGDLSNNKGINRQGGGLSAPALTAKDMDDIKTA
EEEEECCEEEEEEHHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MALGADYVAVSFPKNATDMEMARQLAAVAGQPYGRKARMIAKIERAEAINPGVLEEILQA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHC
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMLSAETAAGRYPVETVEAMAAVCIEAEKSEVVQLDTDFLNQTFSRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
QSVAMGALFTAYHLQVKAIAALTDSGATALWMSRHRIHVPIYAMTPNVASQRKMQLYRNV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCC
VPLPLDASTDRDTALEQAEELLLAQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TRSTKIVATIGPASSSLEVLTRMIAAGVDVVRLNFSHGTAQDHIDRATLVREAAQACGR
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFESGKVTLKPGDPFVLDSSCQLGNQERVGLDYQDLPRDVGPGD
EEEEEEECCCCEEEEEEECCCEEEECCCCCEEECCCCCCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLVVDRVLGTEIFTTVRIGGDLSNNKGINRQGGGLSAPALTAKDMDDIKTA
EEEEECCEEEEEEHHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MALGADYVAVSFPKNATDMEMARQLAAVAGQPYGRKARMIAKIERAEAINPGVLEEILQA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHC
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMLSAETAAGRYPVETVEAMAAVCIEAEKSEVVQLDTDFLNQTFSRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
QSVAMGALFTAYHLQVKAIAALTDSGATALWMSRHRIHVPIYAMTPNVASQRKMQLYRNV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCC
VPLPLDASTDRDTALEQAEELLLAQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]