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The map label for this gene is dctD [H]

Identifier: 73539981

GI number: 73539981

Start: 306152

End: 307477

Strand: Reverse

Name: dctD [H]

Synonym: Reut_A0275

Alternate gene names: 73539981

Gene position: 307477-306152 (Counterclockwise)

Preceding gene: 73539982

Following gene: 73539975

Centisome position: 8.08

GC content: 67.42

Gene sequence:

>1326_bases
ATGCAAGACGGTTTGCGCGTCCTCTTCGTCGAAGATGAACCGCTGGTGCGCCAGGCCACCGCGCAAAGCCTGGAGCTGGC
CGGCTTTTCCGTGCTCGCGCTGCCCACGGCCGAAGCTGCCCTGCCCCACTTGAGCGCCGATTTTCCGGGCGTGCTCGTGA
CCGACGTGCGCCTGCCCGGCGCGAGCGGTCTCGAACTGCTGCATCACTGCCGCAACGCCGCGCCCGGCGTGCCGGTGATA
CTGGTCACGGGACACGGCGACATCACCATGGCGGTGCAGGCCATGCGTGAGGGCGCGTACGACTTCATCGAAAAGCCCTT
CGGCGCCGACCGCCTGACCGAGACCGTGCGCCGCGCCATCGAACGGCGCGGGCTCGAACTGGAAAACCAGGCACTGCGCC
GCGAACTGGCGGGGTCGGCCGCCGGCACGCGCATCATCGGCCGCTCGCATGCGATCGATCAGGTACGTGGGCTCGTGGCC
AATGTCGCGGCCACCGACGTGCCGGTCATGATCAACGGCGAGACCGGCACCGGCAAGGAACTGGTCGCGCGCAGCCTGCA
TGCGTTGTCCAACCGCGCGGACGGCCCGTTCATCGCGCTCAATTGCGGTGCCGTCCCGGAATCGATATTCGAAAGCGAGA
TGTTCGGCCACGAAGCCGGCGCTTTCACCGGCGCCGGCAAGCGCCGCATCGGCAAGCTCGAACATGCGTCGGGCGGCACG
CTGTTCCTCGACGAGATCGAAAGCATGCCGCTGGCGCTGCAGGTGAAGCTGCTACGCGTGCTGCAGGAAGGTACGCTGGA
ACGGCTCGGGTCCAATGCGTCGGTGAAGATCGACGTGCGCATCGTAGCCGCCGCCAAGGGCGACATGGACGCCTTGGTCG
CGCAGGGCGTGTTCCGGCAGGATCTCTACTACCGCCTGAACGTCGTCACCATCGCGCTGCCGCCGCTGCGTGAGCGGCGT
GAAGATATCGTGCCGCTGTTCGAGCATTTCATGCTCGTCGCGGCGGTGCGCTATCAACGGCCTGCGCCCATTCTTTCGGA
GTCGCAGCGCCAGGCGCTGGCGCAGCGGCCGTGGCCCGGCAATGTGCGCGAGCTGCGCAATGCGGCTGACCGGCTAGTGC
TGGGCGTCCCCGAAGGCGGGCAGTCCGGGGCGACCACGGCAGCACCGGATGAGAGCGCGCCGCTGCGGGAGCGCATGGAG
CACTTCGAGCGTGCCGTAATCGCTGATGCGCTGGCCCGCACCGGCGGTGCGGTCAGCCAGGCGGCAGACTTGCTGCAGGT
CGGCAAGGCAACGCTGTATGACAAGATCAAGCGTTATGGCTTATAG

Upstream 100 bases:

>100_bases
CCGCCAATGCTGACGGCGGCGGCGCGGTATTCACCATCGTGCTGACGCGCGCACCGGTCGACGCCGGCGCAGGCGTCACC
CAGACACATTGAGGCATCCC

Downstream 100 bases:

>100_bases
GAAACCTGCTACATAACCTGTCTCAGGGTTTTCACTACGCTGCCATGTCAACGTCACAATCGGCGGCTATATTTCGTGTG
CGGTTGCGGCATTGGCAGGT

Product: helix-turn-helix, Fis-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQDGLRVLFVEDEPLVRQATAQSLELAGFSVLALPTAEAALPHLSADFPGVLVTDVRLPGASGLELLHHCRNAAPGVPVI
LVTGHGDITMAVQAMREGAYDFIEKPFGADRLTETVRRAIERRGLELENQALRRELAGSAAGTRIIGRSHAIDQVRGLVA
NVAATDVPVMINGETGTGKELVARSLHALSNRADGPFIALNCGAVPESIFESEMFGHEAGAFTGAGKRRIGKLEHASGGT
LFLDEIESMPLALQVKLLRVLQEGTLERLGSNASVKIDVRIVAAAKGDMDALVAQGVFRQDLYYRLNVVTIALPPLRERR
EDIVPLFEHFMLVAAVRYQRPAPILSESQRQALAQRPWPGNVRELRNAADRLVLGVPEGGQSGATTAAPDESAPLRERME
HFERAVIADALARTGGAVSQAADLLQVGKATLYDKIKRYGL

Sequences:

>Translated_441_residues
MQDGLRVLFVEDEPLVRQATAQSLELAGFSVLALPTAEAALPHLSADFPGVLVTDVRLPGASGLELLHHCRNAAPGVPVI
LVTGHGDITMAVQAMREGAYDFIEKPFGADRLTETVRRAIERRGLELENQALRRELAGSAAGTRIIGRSHAIDQVRGLVA
NVAATDVPVMINGETGTGKELVARSLHALSNRADGPFIALNCGAVPESIFESEMFGHEAGAFTGAGKRRIGKLEHASGGT
LFLDEIESMPLALQVKLLRVLQEGTLERLGSNASVKIDVRIVAAAKGDMDALVAQGVFRQDLYYRLNVVTIALPPLRERR
EDIVPLFEHFMLVAAVRYQRPAPILSESQRQALAQRPWPGNVRELRNAADRLVLGVPEGGQSGATTAAPDESAPLRERME
HFERAVIADALARTGGAVSQAADLLQVGKATLYDKIKRYGL
>Mature_441_residues
MQDGLRVLFVEDEPLVRQATAQSLELAGFSVLALPTAEAALPHLSADFPGVLVTDVRLPGASGLELLHHCRNAAPGVPVI
LVTGHGDITMAVQAMREGAYDFIEKPFGADRLTETVRRAIERRGLELENQALRRELAGSAAGTRIIGRSHAIDQVRGLVA
NVAATDVPVMINGETGTGKELVARSLHALSNRADGPFIALNCGAVPESIFESEMFGHEAGAFTGAGKRRIGKLEHASGGT
LFLDEIESMPLALQVKLLRVLQEGTLERLGSNASVKIDVRIVAAAKGDMDALVAQGVFRQDLYYRLNVVTIALPPLRERR
EDIVPLFEHFMLVAAVRYQRPAPILSESQRQALAQRPWPGNVRELRNAADRLVLGVPEGGQSGATTAAPDESAPLRERME
HFERAVIADALARTGGAVSQAADLLQVGKATLYDKIKRYGL

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=39.6017699115044, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=40.5919661733615, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1790437, Length=445, Percent_Identity=37.3033707865169, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=37.3873873873874, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=42.814371257485, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI87082117, Length=341, Percent_Identity=39.8826979472141, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=42.3197492163009, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=47.3913043478261, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=38.3435582822086, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=37.202380952381, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87081858, Length=290, Percent_Identity=32.7586206896552, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789828, Length=269, Percent_Identity=34.9442379182156, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=127, Percent_Identity=29.9212598425197, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47447; Mature: 47447

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDGLRVLFVEDEPLVRQATAQSLELAGFSVLALPTAEAALPHLSADFPGVLVTDVRLPG
CCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCCCCEEEEEEECCC
ASGLELLHHCRNAAPGVPVILVTGHGDITMAVQAMREGAYDFIEKPFGADRLTETVRRAI
CCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHH
ERRGLELENQALRRELAGSAAGTRIIGRSHAIDQVRGLVANVAATDVPVMINGETGTGKE
HHCCCCHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHH
LVARSLHALSNRADGPFIALNCGAVPESIFESEMFGHEAGAFTGAGKRRIGKLEHASGGT
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCE
LFLDEIESMPLALQVKLLRVLQEGTLERLGSNASVKIDVRIVAAAKGDMDALVAQGVFRQ
EEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
DLYYRLNVVTIALPPLRERREDIVPLFEHFMLVAAVRYQRPAPILSESQRQALAQRPWPG
HHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
NVRELRNAADRLVLGVPEGGQSGATTAAPDESAPLRERMEHFERAVIADALARTGGAVSQ
CHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
AADLLQVGKATLYDKIKRYGL
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDGLRVLFVEDEPLVRQATAQSLELAGFSVLALPTAEAALPHLSADFPGVLVTDVRLPG
CCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCCCCEEEEEEECCC
ASGLELLHHCRNAAPGVPVILVTGHGDITMAVQAMREGAYDFIEKPFGADRLTETVRRAI
CCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHH
ERRGLELENQALRRELAGSAAGTRIIGRSHAIDQVRGLVANVAATDVPVMINGETGTGKE
HHCCCCHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHH
LVARSLHALSNRADGPFIALNCGAVPESIFESEMFGHEAGAFTGAGKRRIGKLEHASGGT
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCE
LFLDEIESMPLALQVKLLRVLQEGTLERLGSNASVKIDVRIVAAAKGDMDALVAQGVFRQ
EEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
DLYYRLNVVTIALPPLRERREDIVPLFEHFMLVAAVRYQRPAPILSESQRQALAQRPWPG
HHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
NVRELRNAADRLVLGVPEGGQSGATTAAPDESAPLRERMEHFERAVIADALARTGGAVSQ
CHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
AADLLQVGKATLYDKIKRYGL
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]