Click here to switch to the map view.

The map label for this gene is paaK [H]

Identifier: 73539972

GI number: 73539972

Start: 296141

End: 297391

Strand: Direct

Name: paaK [H]

Synonym: Reut_A0266

Alternate gene names: 73539972

Gene position: 296141-297391 (Clockwise)

Preceding gene: 73539971

Following gene: 73539976

Centisome position: 7.78

GC content: 67.15

Gene sequence:

>1251_bases
ATGCCTGAATACTTCGATTCGCTCGAAACCCGTGCGCCGGAAGTTCGCGAGCAGGCGCTGCTGGCCGCACTGTCCCGCCA
GGTAGCCCATGCCCGTGCGCATGCGCCTTACTTCGCGGAACTTCTGCGCGACGTGGATCCCGCCACGCTGACCACGCGCG
AAGCGCTGGCCGCGCTGCCGGTAACACGCAAGTCGGACCTGACCGCGCGGCAAAAGGCCATGCCGCCGCTGGGCGGCCTA
AATGCCACGCCGATCGGCGGCCTGCGCCACATCTTCCAGTCGCCGGGCCCGATCCACGAGCCCGATGGCCACGACGCCGA
CTGGTGGCGTATCGCACGCGCCATGTTCGCGGCTGGGTTCCGCGCTGGCGAACTGGTCTACAACACCTTCTCCTATCACT
TCACGCCGGCCGGCATGATGATGGAGACGGGCGCGCATCGGCTCGGCTGCTGCGTGTTCCCCGCAGGTGTCGGACAAACC
GAGTCGCAGGTGCAGGCGCTGGCCAGCCTGCAGCCGTCCGCCTACGCGGGCACGCCGTCGTTTCTCAAGCTGCTGCTGGA
GCGTGGCGCGGAAATGGGCACGCCATGCCACAGCCTGTCCAAAGCGCTGGTCTCTGGCGAGGCATTGCCGCCATCGCTGC
GCGGCTGGTTCCAGGAGCGCGGCATCCGCGTGCAGCAGATGTATGGCTCGGCCGATCTCGGACTCATCGCTTACGAAACG
GACGGCGGCGATGGATGGGTCGTCGATGAAAACGTGCTCGTCGAGATCGTCGAACCGGGCGGCACGCGGCCGATGCCCGA
AGGCGAGACCGGCGAGGTCGTGGTGACCGTGCTCGGCAATGGCGACTATCCGTTGATCCGCTTTGGTACGGGCGACCTGT
CGGCGATCGTCGCCGATTCTTCGCATCGCGCGAGCCCTTGCGGGCGCACCAATATCCGCCTTAAGGGCTGGCTCGGGCGC
GCCGACCAGACTACCAAAGTCAAGGGCATGTTCGTGCATCCGGGGCAAGTCGCGGACGTGCTGCGCCGCCATCCGGAAAT
CCGGGCTGCGCGGCTGGTCGTGACCGGAACCATGGGTGCCGACGTGATGACGCTGCATTGCGACGCCGCTCGCGACGACG
ACGGGCTGAAGCAGGCCGTGGCGGAGTCGTTGCGCGAAGTGATGCGCCTGCGCGGCGAGGTGGCGTTCGTGCAGGCAGGG
TCATTGCCGCAGGACGGCAAGGTAATCGAGGACGCCCGCCGCTACGATTAG

Upstream 100 bases:

>100_bases
CTGGCTGGCGTGACGCACTCCGGTTGCTTGCCACGTCCCCTCAGCGGAAGAGGGGCGCAACACCCGCCGAATTCACCGAA
CTTATTCGTCGTATCCCACC

Downstream 100 bases:

>100_bases
CGGTTTAATTGCCGTGCCCGCCCGGCGGCCGGGTGGGCAGGCGCAGCATCCAGATGCTGACGGCGGCCGCACACGCAATC
AGGGCGGCCGACGCCAGAGG

Product: putative phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MPEYFDSLETRAPEVREQALLAALSRQVAHARAHAPYFAELLRDVDPATLTTREALAALPVTRKSDLTARQKAMPPLGGL
NATPIGGLRHIFQSPGPIHEPDGHDADWWRIARAMFAAGFRAGELVYNTFSYHFTPAGMMMETGAHRLGCCVFPAGVGQT
ESQVQALASLQPSAYAGTPSFLKLLLERGAEMGTPCHSLSKALVSGEALPPSLRGWFQERGIRVQQMYGSADLGLIAYET
DGGDGWVVDENVLVEIVEPGGTRPMPEGETGEVVVTVLGNGDYPLIRFGTGDLSAIVADSSHRASPCGRTNIRLKGWLGR
ADQTTKVKGMFVHPGQVADVLRRHPEIRAARLVVTGTMGADVMTLHCDAARDDDGLKQAVAESLREVMRLRGEVAFVQAG
SLPQDGKVIEDARRYD

Sequences:

>Translated_416_residues
MPEYFDSLETRAPEVREQALLAALSRQVAHARAHAPYFAELLRDVDPATLTTREALAALPVTRKSDLTARQKAMPPLGGL
NATPIGGLRHIFQSPGPIHEPDGHDADWWRIARAMFAAGFRAGELVYNTFSYHFTPAGMMMETGAHRLGCCVFPAGVGQT
ESQVQALASLQPSAYAGTPSFLKLLLERGAEMGTPCHSLSKALVSGEALPPSLRGWFQERGIRVQQMYGSADLGLIAYET
DGGDGWVVDENVLVEIVEPGGTRPMPEGETGEVVVTVLGNGDYPLIRFGTGDLSAIVADSSHRASPCGRTNIRLKGWLGR
ADQTTKVKGMFVHPGQVADVLRRHPEIRAARLVVTGTMGADVMTLHCDAARDDDGLKQAVAESLREVMRLRGEVAFVQAG
SLPQDGKVIEDARRYD
>Mature_415_residues
PEYFDSLETRAPEVREQALLAALSRQVAHARAHAPYFAELLRDVDPATLTTREALAALPVTRKSDLTARQKAMPPLGGLN
ATPIGGLRHIFQSPGPIHEPDGHDADWWRIARAMFAAGFRAGELVYNTFSYHFTPAGMMMETGAHRLGCCVFPAGVGQTE
SQVQALASLQPSAYAGTPSFLKLLLERGAEMGTPCHSLSKALVSGEALPPSLRGWFQERGIRVQQMYGSADLGLIAYETD
GGDGWVVDENVLVEIVEPGGTRPMPEGETGEVVVTVLGNGDYPLIRFGTGDLSAIVADSSHRASPCGRTNIRLKGWLGRA
DQTTKVKGMFVHPGQVADVLRRHPEIRAARLVVTGTMGADVMTLHCDAARDDDGLKQAVAESLREVMRLRGEVAFVQAGS
LPQDGKVIEDARRYD

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=355, Percent_Identity=27.3239436619718, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 44932; Mature: 44800

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYFDSLETRAPEVREQALLAALSRQVAHARAHAPYFAELLRDVDPATLTTREALAALP
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCC
VTRKSDLTARQKAMPPLGGLNATPIGGLRHIFQSPGPIHEPDGHDADWWRIARAMFAAGF
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
RAGELVYNTFSYHFTPAGMMMETGAHRLGCCVFPAGVGQTESQVQALASLQPSAYAGTPS
CCCCEEEEEEEEEECCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHH
FLKLLLERGAEMGTPCHSLSKALVSGEALPPSLRGWFQERGIRVQQMYGSADLGLIAYET
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEEE
DGGDGWVVDENVLVEIVEPGGTRPMPEGETGEVVVTVLGNGDYPLIRFGTGDLSAIVADS
CCCCCEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEECC
SHRASPCGRTNIRLKGWLGRADQTTKVKGMFVHPGQVADVLRRHPEIRAARLVVTGTMGA
CCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCC
DVMTLHCDAARDDDGLKQAVAESLREVMRLRGEVAFVQAGSLPQDGKVIEDARRYD
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
PEYFDSLETRAPEVREQALLAALSRQVAHARAHAPYFAELLRDVDPATLTTREALAALP
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCC
VTRKSDLTARQKAMPPLGGLNATPIGGLRHIFQSPGPIHEPDGHDADWWRIARAMFAAGF
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
RAGELVYNTFSYHFTPAGMMMETGAHRLGCCVFPAGVGQTESQVQALASLQPSAYAGTPS
CCCCEEEEEEEEEECCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHH
FLKLLLERGAEMGTPCHSLSKALVSGEALPPSLRGWFQERGIRVQQMYGSADLGLIAYET
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEEE
DGGDGWVVDENVLVEIVEPGGTRPMPEGETGEVVVTVLGNGDYPLIRFGTGDLSAIVADS
CCCCCEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEECC
SHRASPCGRTNIRLKGWLGRADQTTKVKGMFVHPGQVADVLRRHPEIRAARLVVTGTMGA
CCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCC
DVMTLHCDAARDDDGLKQAVAESLREVMRLRGEVAFVQAGSLPQDGKVIEDARRYD
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]