BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

Click here to switch to the map view.

The map label for this gene is braG [H]

Identifier: 73539971

GI number: 73539971

Start: 295238

End: 296053

Strand: Direct

Name: braG [H]

Synonym: Reut_A0265

Alternate gene names: 73539971

Gene position: 295238-296053 (Clockwise)

Preceding gene: 73539970

Following gene: 73539972

Centisome position: 7.76

GC content: 64.71

Gene sequence:

>816_bases
ATGACCCTTCTCTCCGTCAACAATATCGAAGTCATCTACGATCACGTGATCCTGGTGCTCAAGGGCGTATCGCTCGAAGT
GCCGGAGGGCAAGATCGTCGCGCTGCTGGGCGCCAACGGTGCGGGCAAGACCACCACGCTCAAGGCCATCTCCAACCTGC
TGCATGCGGAGCGCGGTGACGTCACCAAGGGCTCGGTCGAATACCGCGGCGACCGCGTCGACCAGCTCACGCCGAACGAC
CTGGTCCGGCGTGGCGTGATCCAGGTGATGGAGGGCCGCCACTGCTTCGCCCACCTCACCATCGAAGAGAACCTGCTGAC
CGGAGCCTACACGCGCGGCCTGTCGCGCGGAGAAACGCGCGACGAGCTGGAGAAGATCTACGCCTACTTCCCGCGCCTGA
AGACGCGCCGCAAGTCGCAGGCGGGCTATACCTCCGGCGGCGAGCAGCAGATGTGCGCGATCGGCCGGGCCATGATGGCC
AAGCCCGCGATGATCCTGCTCGACGAGCCGTCGATGGGCCTCGCGCCGCAGATCGTCGAGGAGATCTTCGAGATCGTGCG
CGACCTGAACACGCGTGAGAAGGTCAGCTTCCTGCTGGCCGAGCAGAACACCATGGTGGCGCTGCGCTATGCCGACTACG
GCTACATCCTGGAGAACGGCCGCGTGGTGATGGACGGCGACGCCGAGTCGCTGCGCACCAATGAGGACGTCAAGGAGTTC
TACCTTGGCGTGGCCGCCAACGACGCGGAGGGTGGAGAGCGCAAGTCGTTCCGCGATGTGAAGAGCTATCGCCGCAGAAA
GCGCTGGCTGGCGTGA

Upstream 100 bases:

>100_bases
CATGAAGTCGTGATTGCTGCGGCAGTGAACGCTGCCAGTTGTGTGCTCTCCGCTCGTGCGTGCACGGGCGGAGAGGCCCC
AGACATTTGAGAGCCGTACC

Downstream 100 bases:

>100_bases
CGCACTCCGGTTGCTTGCCACGTCCCCTCAGCGGAAGAGGGGCGCAACACCCGCCGAATTCACCGAACTTATTCGTCGTA
TCCCACCATGCCTGAATACT

Product: ABC transporter related

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTLLSVNNIEVIYDHVILVLKGVSLEVPEGKIVALLGANGAGKTTTLKAISNLLHAERGDVTKGSVEYRGDRVDQLTPND
LVRRGVIQVMEGRHCFAHLTIEENLLTGAYTRGLSRGETRDELEKIYAYFPRLKTRRKSQAGYTSGGEQQMCAIGRAMMA
KPAMILLDEPSMGLAPQIVEEIFEIVRDLNTREKVSFLLAEQNTMVALRYADYGYILENGRVVMDGDAESLRTNEDVKEF
YLGVAANDAEGGERKSFRDVKSYRRRKRWLA

Sequences:

>Translated_271_residues
MTLLSVNNIEVIYDHVILVLKGVSLEVPEGKIVALLGANGAGKTTTLKAISNLLHAERGDVTKGSVEYRGDRVDQLTPND
LVRRGVIQVMEGRHCFAHLTIEENLLTGAYTRGLSRGETRDELEKIYAYFPRLKTRRKSQAGYTSGGEQQMCAIGRAMMA
KPAMILLDEPSMGLAPQIVEEIFEIVRDLNTREKVSFLLAEQNTMVALRYADYGYILENGRVVMDGDAESLRTNEDVKEF
YLGVAANDAEGGERKSFRDVKSYRRRKRWLA
>Mature_270_residues
TLLSVNNIEVIYDHVILVLKGVSLEVPEGKIVALLGANGAGKTTTLKAISNLLHAERGDVTKGSVEYRGDRVDQLTPNDL
VRRGVIQVMEGRHCFAHLTIEENLLTGAYTRGLSRGETRDELEKIYAYFPRLKTRRKSQAGYTSGGEQQMCAIGRAMMAK
PAMILLDEPSMGLAPQIVEEIFEIVRDLNTREKVSFLLAEQNTMVALRYADYGYILENGRVVMDGDAESLRTNEDVKEFY
LGVAANDAEGGERKSFRDVKSYRRRKRWLA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=213, Percent_Identity=27.2300469483568, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI148612844, Length=230, Percent_Identity=26.9565217391304, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI255708477, Length=230, Percent_Identity=26.9565217391304, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21536376, Length=248, Percent_Identity=25.4032258064516, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI27436953, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI42490749, Length=235, Percent_Identity=28.936170212766, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI27477115, Length=213, Percent_Identity=27.6995305164319, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI46592915, Length=215, Percent_Identity=26.046511627907, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI46592971, Length=215, Percent_Identity=26.046511627907, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI46592978, Length=215, Percent_Identity=26.046511627907, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI46592956, Length=215, Percent_Identity=26.046511627907, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI46592964, Length=215, Percent_Identity=26.046511627907, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI46592898, Length=215, Percent_Identity=26.046511627907, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI105990541, Length=204, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI9961252, Length=229, Percent_Identity=28.82096069869, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI9961250, Length=229, Percent_Identity=28.82096069869, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4505771, Length=229, Percent_Identity=28.82096069869, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI42741659, Length=235, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI45446740, Length=231, Percent_Identity=26.8398268398268, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI47078218, Length=231, Percent_Identity=26.8398268398268, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI116734710, Length=155, Percent_Identity=30.3225806451613, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27262624, Length=157, Percent_Identity=29.2993630573248, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27262626, Length=157, Percent_Identity=29.2993630573248, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27881501, Length=230, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI150417984, Length=213, Percent_Identity=27.6995305164319, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082268, Length=244, Percent_Identity=40.9836065573771, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789864, Length=257, Percent_Identity=28.4046692607004, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787089, Length=218, Percent_Identity=32.5688073394495, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788225, Length=259, Percent_Identity=31.2741312741313, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787029, Length=233, Percent_Identity=28.755364806867, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789593, Length=227, Percent_Identity=30.8370044052863, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789672, Length=222, Percent_Identity=27.9279279279279, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788450, Length=253, Percent_Identity=28.0632411067194, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87082267, Length=220, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786398, Length=226, Percent_Identity=27.8761061946903, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787792, Length=226, Percent_Identity=27.4336283185841, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788761, Length=233, Percent_Identity=30.4721030042918, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787712, Length=220, Percent_Identity=27.7272727272727, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786253, Length=251, Percent_Identity=25.8964143426295, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081709, Length=213, Percent_Identity=25.3521126760563, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790544, Length=241, Percent_Identity=28.6307053941909, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788644, Length=227, Percent_Identity=28.6343612334802, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1790467, Length=227, Percent_Identity=28.6343612334802, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081791, Length=231, Percent_Identity=25.5411255411255, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789586, Length=239, Percent_Identity=25.5230125523013, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787105, Length=198, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786872, Length=231, Percent_Identity=26.8398268398268, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788506, Length=246, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786319, Length=246, Percent_Identity=26.4227642276423, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789032, Length=234, Percent_Identity=25.2136752136752, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787370, Length=208, Percent_Identity=25.4807692307692, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI48994943, Length=266, Percent_Identity=25.187969924812, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48995001, Length=211, Percent_Identity=29.3838862559242, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786803, Length=226, Percent_Identity=24.7787610619469, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790739, Length=223, Percent_Identity=28.2511210762332, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790162, Length=258, Percent_Identity=23.6434108527132, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789873, Length=188, Percent_Identity=28.7234042553192, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994997, Length=237, Percent_Identity=28.2700421940928, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081834, Length=197, Percent_Identity=27.4111675126904, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787972, Length=235, Percent_Identity=26.3829787234043, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786703, Length=209, Percent_Identity=27.7511961722488, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790525, Length=230, Percent_Identity=25.6521739130435, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790190, Length=193, Percent_Identity=25.3886010362694, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788472, Length=179, Percent_Identity=26.2569832402235, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787112, Length=211, Percent_Identity=28.9099526066351, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788897, Length=191, Percent_Identity=28.2722513089005, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787164, Length=211, Percent_Identity=24.1706161137441, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=237, Percent_Identity=26.5822784810127, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI193211017, Length=232, Percent_Identity=29.3103448275862, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI193211015, Length=232, Percent_Identity=29.3103448275862, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71996809, Length=232, Percent_Identity=29.3103448275862, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=236, Percent_Identity=25.4237288135593, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212646699, Length=220, Percent_Identity=27.7272727272727, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17541710, Length=244, Percent_Identity=26.6393442622951, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193209708, Length=219, Percent_Identity=26.4840182648402, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558664, Length=238, Percent_Identity=26.0504201680672, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=236, Percent_Identity=24.5762711864407, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=236, Percent_Identity=24.5762711864407, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71996550, Length=213, Percent_Identity=27.2300469483568, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=236, Percent_Identity=24.5762711864407, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17567265, Length=223, Percent_Identity=26.457399103139, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17567269, Length=223, Percent_Identity=26.457399103139, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115535101, Length=223, Percent_Identity=26.0089686098655, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17569143, Length=221, Percent_Identity=27.6018099547511, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI85726357, Length=224, Percent_Identity=26.3392857142857, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28574150, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007328, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24666092, Length=218, Percent_Identity=24.3119266055046, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641565, Length=222, Percent_Identity=26.5765765765766, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28574533, Length=216, Percent_Identity=24.537037037037, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 30347; Mature: 30216

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLSVNNIEVIYDHVILVLKGVSLEVPEGKIVALLGANGAGKTTTLKAISNLLHAERGD
CEEEECCCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
VTKGSVEYRGDRVDQLTPNDLVRRGVIQVMEGRHCFAHLTIEENLLTGAYTRGLSRGETR
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCCCH
DELEKIYAYFPRLKTRRKSQAGYTSGGEQQMCAIGRAMMAKPAMILLDEPSMGLAPQIVE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
EIFEIVRDLNTREKVSFLLAEQNTMVALRYADYGYILENGRVVMDGDAESLRTNEDVKEF
HHHHHHHCCCCHHHHHEEEECCCCEEEEEECCCEEEEECCEEEECCCHHHHCCCHHHHHH
YLGVAANDAEGGERKSFRDVKSYRRRKRWLA
HHEEECCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLLSVNNIEVIYDHVILVLKGVSLEVPEGKIVALLGANGAGKTTTLKAISNLLHAERGD
EEEECCCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
VTKGSVEYRGDRVDQLTPNDLVRRGVIQVMEGRHCFAHLTIEENLLTGAYTRGLSRGETR
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCCCCH
DELEKIYAYFPRLKTRRKSQAGYTSGGEQQMCAIGRAMMAKPAMILLDEPSMGLAPQIVE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
EIFEIVRDLNTREKVSFLLAEQNTMVALRYADYGYILENGRVVMDGDAESLRTNEDVKEF
HHHHHHHCCCCHHHHHEEEECCCCEEEEEECCCEEEEECCEEEECCCHHHHCCCHHHHHH
YLGVAANDAEGGERKSFRDVKSYRRRKRWLA
HHEEECCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]