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The map label for this gene is dmpD [H]

Identifier: 73539512

GI number: 73539512

Start: 2520898

End: 2521749

Strand: Direct

Name: dmpD [H]

Synonym: Reut_B5690

Alternate gene names: 73539512

Gene position: 2520898-2521749 (Clockwise)

Preceding gene: 73539511

Following gene: 73539513

Centisome position: 92.47

GC content: 67.25

Gene sequence:

>852_bases
ATGAGCGAAAGCGATTTTCAGGTACCCGAGACCCGCTACGTGAGAACAGGTGCGGTGACAACGCACATCCATGACGTTGG
CCGGGGTTCGCCCGTGCTGCTGCTGCACGGATCCGGCCCAGGGGTCAGCGCGTGGGCCAACTGGCGCCCGGTGCTGCCGG
CGCTGGCCCGTGAACGCCGCGTCATCGCGCCGGACATGCTTGGCTTCGGCCAGAGCGACCGTCCGGCCGGACAGGTCTAC
GGCATGGACGTCTGGGTGCAGCAGGCCCTGGACTTGCTCGATGCACTGGAACTGCCGCAGGTCGATCTCGTCGGCAACTC
GTTTGGCGGGGCGCTGGCGCTGGCGCTTGCCATCCGTGCACCGGGGCGGGTGCGGCGGCTTGTGCTGATGGGATCGGTCG
GGGTGCCATTCCGCATCACGTCGGGGCTGGAGGCAGTGTGGGGCTATGAGCCGTCACTGACGGCAATGCGGGGGCTGCTG
GACCTGTTCGCCTACGACCGTGGCCGTGTGACCGACGAACTGGCCCGGCTGCGGTACGAAGCCAGCGTGGCACCTGGCGT
GCAGGAATCCTATGGCGCCATGTTTCCCGCGCCGCGCCAGCGGTGGGTCGACGCGATGGCGAGCGCCGAGGCGGATATTC
GCGCGCTGCCGCACGAGACGCTGGTGGTCCACGGCAGGGAAGACAAGGTCATCCCGCTGTCTAATTCGCTGACGCTGGCG
GACTGGATTCCCAACAGCCAGCTCCATGTATTCGGCCGTTGCGGCCACTGGACACAGATAGAGCACAGCGCGCGCTTCGC
GACGCTGGTGAGCAACTTCCTGGCCGAAGCCGACGCCGCTGCAGTCGCTTGA

Upstream 100 bases:

>100_bases
GCACGGCATTCGGCGGGGCCAAGGCGTCGGGTATCGGTCGTGAGGGCGGCGTGCATTCCCTGGAGTTCTACACCGAACTG
CGCAATGTGATGGTGAAGCT

Downstream 100 bases:

>100_bases
CCCGAATTTCACGTATCGAGGAACACTCATGGACGCCATAAAGACCCAACACTACGGCGACGCGCTCTACGAATCGCTCC
TGACGCGCAAGCCGCTGGAA

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSESDFQVPETRYVRTGAVTTHIHDVGRGSPVLLLHGSGPGVSAWANWRPVLPALARERRVIAPDMLGFGQSDRPAGQVY
GMDVWVQQALDLLDALELPQVDLVGNSFGGALALALAIRAPGRVRRLVLMGSVGVPFRITSGLEAVWGYEPSLTAMRGLL
DLFAYDRGRVTDELARLRYEASVAPGVQESYGAMFPAPRQRWVDAMASAEADIRALPHETLVVHGREDKVIPLSNSLTLA
DWIPNSQLHVFGRCGHWTQIEHSARFATLVSNFLAEADAAAVA

Sequences:

>Translated_283_residues
MSESDFQVPETRYVRTGAVTTHIHDVGRGSPVLLLHGSGPGVSAWANWRPVLPALARERRVIAPDMLGFGQSDRPAGQVY
GMDVWVQQALDLLDALELPQVDLVGNSFGGALALALAIRAPGRVRRLVLMGSVGVPFRITSGLEAVWGYEPSLTAMRGLL
DLFAYDRGRVTDELARLRYEASVAPGVQESYGAMFPAPRQRWVDAMASAEADIRALPHETLVVHGREDKVIPLSNSLTLA
DWIPNSQLHVFGRCGHWTQIEHSARFATLVSNFLAEADAAAVA
>Mature_282_residues
SESDFQVPETRYVRTGAVTTHIHDVGRGSPVLLLHGSGPGVSAWANWRPVLPALARERRVIAPDMLGFGQSDRPAGQVYG
MDVWVQQALDLLDALELPQVDLVGNSFGGALALALAIRAPGRVRRLVLMGSVGVPFRITSGLEAVWGYEPSLTAMRGLLD
LFAYDRGRVTDELARLRYEASVAPGVQESYGAMFPAPRQRWVDAMASAEADIRALPHETLVVHGREDKVIPLSNSLTLAD
WIPNSQLHVFGRCGHWTQIEHSARFATLVSNFLAEADAAAVA

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=308, Percent_Identity=25.6493506493506, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI87081721, Length=266, Percent_Identity=34.5864661654135, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30739; Mature: 30608

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESDFQVPETRYVRTGAVTTHIHDVGRGSPVLLLHGSGPGVSAWANWRPVLPALARERR
CCCCCCCCCCCEEEECCCEEEEEHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCC
VIAPDMLGFGQSDRPAGQVYGMDVWVQQALDLLDALELPQVDLVGNSFGGALALALAIRA
CCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCC
PGRVRRLVLMGSVGVPFRITSGLEAVWGYEPSLTAMRGLLDLFAYDRGRVTDELARLRYE
CCHHEEEEEECCCCCCEEECCCCHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ASVAPGVQESYGAMFPAPRQRWVDAMASAEADIRALPHETLVVHGREDKVIPLSNSLTLA
HHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCEEECCCCEEEE
DWIPNSQLHVFGRCGHWTQIEHSARFATLVSNFLAEADAAAVA
ECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SESDFQVPETRYVRTGAVTTHIHDVGRGSPVLLLHGSGPGVSAWANWRPVLPALARERR
CCCCCCCCCCEEEECCCEEEEEHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCC
VIAPDMLGFGQSDRPAGQVYGMDVWVQQALDLLDALELPQVDLVGNSFGGALALALAIRA
CCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCC
PGRVRRLVLMGSVGVPFRITSGLEAVWGYEPSLTAMRGLLDLFAYDRGRVTDELARLRYE
CCHHEEEEEECCCCCCEEECCCCHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ASVAPGVQESYGAMFPAPRQRWVDAMASAEADIRALPHETLVVHGREDKVIPLSNSLTLA
HHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCEEECCCCEEEE
DWIPNSQLHVFGRCGHWTQIEHSARFATLVSNFLAEADAAAVA
ECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194577 [H]