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The map label for this gene is xylJ [H]

Identifier: 73539513

GI number: 73539513

Start: 2521778

End: 2522560

Strand: Direct

Name: xylJ [H]

Synonym: Reut_B5691

Alternate gene names: 73539513

Gene position: 2521778-2522560 (Clockwise)

Preceding gene: 73539512

Following gene: 73539514

Centisome position: 92.5

GC content: 65.52

Gene sequence:

>783_bases
ATGGACGCCATAAAGACCCAACACTACGGCGACGCGCTCTACGAATCGCTCCTGACGCGCAAGCCGCTGGAACCACTCAC
CGACCGCGAGCCGGACATCACCATCGAAGACGCCTACCAGATCCAGTTGCGCATGATCGGTCGCCGCCTGGAAGCGGGCG
AGACCGTGATCGGCAAGAAGATCGGCGTGACCAGCCGGGTGGTGATGGACATGCTCGGGGTGGACCAGCCCGACTTCGGT
CATCTGCTGTCCGGCATGGTCTTCAACGAGGGCGAACCGGTGGACACGGGCAAGATGATCGCGCCGAAGGCGGAGGCAGA
AGTGGCTTTCGTGCTGTCCAGAGACCTCTGCGGGCCGGGCCTGACCGCAGCCGATGTACTGCGTGCTACCGATTGCGTGA
TGCCGTGCTTCGAGATCGTCGATTCCCGCATCCGAGACTGGAAGATTCGCATCCAGGACACCGTGGCCGACAATGCCTCA
TGCGGCGTCTTCACACTCGGCGGCACGCGCCGCAGCCCGCGCGACCTCGATCTCGCGCTGGCAGGCATGGTGCTGGAGAA
GAACGGCGAGATCGTGAGCACCTCGTGCGGCGCCTCGGTGCAAGGCTCGCCCGTCAATGCGGTGGCGTGGCTGGCGAATA
CGCTGGGCCGCTTCGGCATCGCGCTGAAGGCAGGCGACGTGATCCTGTCGGGCTCGCAGTCGCCGCTGGTGCCCGTCAAG
CCGGGCGACAGCCTGTATTGCAGCGTGGGCGGGCTGGGCGGGACCTCGGTGCGCTTCATCTGA

Upstream 100 bases:

>100_bases
GAGCACAGCGCGCGCTTCGCGACGCTGGTGAGCAACTTCCTGGCCGAAGCCGACGCCGCTGCAGTCGCTTGACCCGAATT
TCACGTATCGAGGAACACTC

Downstream 100 bases:

>100_bases
CTCATACCCATCGGGAAACCAGACCATGACATTGAACCGGGAAACCATTGAAGCGCTCGCCGCGCATGTCGAGAGCGCAC
AACTTGCCGTGCGCGACATC

Product: 4-oxalocrotonate decarboxylase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDAIKTQHYGDALYESLLTRKPLEPLTDREPDITIEDAYQIQLRMIGRRLEAGETVIGKKIGVTSRVVMDMLGVDQPDFG
HLLSGMVFNEGEPVDTGKMIAPKAEAEVAFVLSRDLCGPGLTAADVLRATDCVMPCFEIVDSRIRDWKIRIQDTVADNAS
CGVFTLGGTRRSPRDLDLALAGMVLEKNGEIVSTSCGASVQGSPVNAVAWLANTLGRFGIALKAGDVILSGSQSPLVPVK
PGDSLYCSVGGLGGTSVRFI

Sequences:

>Translated_260_residues
MDAIKTQHYGDALYESLLTRKPLEPLTDREPDITIEDAYQIQLRMIGRRLEAGETVIGKKIGVTSRVVMDMLGVDQPDFG
HLLSGMVFNEGEPVDTGKMIAPKAEAEVAFVLSRDLCGPGLTAADVLRATDCVMPCFEIVDSRIRDWKIRIQDTVADNAS
CGVFTLGGTRRSPRDLDLALAGMVLEKNGEIVSTSCGASVQGSPVNAVAWLANTLGRFGIALKAGDVILSGSQSPLVPVK
PGDSLYCSVGGLGGTSVRFI
>Mature_260_residues
MDAIKTQHYGDALYESLLTRKPLEPLTDREPDITIEDAYQIQLRMIGRRLEAGETVIGKKIGVTSRVVMDMLGVDQPDFG
HLLSGMVFNEGEPVDTGKMIAPKAEAEVAFVLSRDLCGPGLTAADVLRATDCVMPCFEIVDSRIRDWKIRIQDTVADNAS
CGVFTLGGTRRSPRDLDLALAGMVLEKNGEIVSTSCGASVQGSPVNAVAWLANTLGRFGIALKAGDVILSGSQSPLVPVK
PGDSLYCSVGGLGGTSVRFI

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=239, Percent_Identity=39.7489539748954, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 27726; Mature: 27726

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAIKTQHYGDALYESLLTRKPLEPLTDREPDITIEDAYQIQLRMIGRRLEAGETVIGKK
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCEECCCC
IGVTSRVVMDMLGVDQPDFGHLLSGMVFNEGEPVDTGKMIAPKAEAEVAFVLSRDLCGPG
CCHHHHHHHHHHCCCCCCHHHHHHCCEECCCCCCCCCCEECCCCCCEEEEEEECCCCCCC
LTAADVLRATDCVMPCFEIVDSRIRDWKIRIQDTVADNASCGVFTLGGTRRSPRDLDLAL
CCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCCCCCCCHHHEE
AGMVLEKNGEIVSTSCGASVQGSPVNAVAWLANTLGRFGIALKAGDVILSGSQSPLVPVK
EEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEECCEEEECCCCCEEEEC
PGDSLYCSVGGLGGTSVRFI
CCCCEEEEECCCCCCEEEEC
>Mature Secondary Structure
MDAIKTQHYGDALYESLLTRKPLEPLTDREPDITIEDAYQIQLRMIGRRLEAGETVIGKK
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCEECCCC
IGVTSRVVMDMLGVDQPDFGHLLSGMVFNEGEPVDTGKMIAPKAEAEVAFVLSRDLCGPG
CCHHHHHHHHHHCCCCCCHHHHHHCCEECCCCCCCCCCEECCCCCCEEEEEEECCCCCCC
LTAADVLRATDCVMPCFEIVDSRIRDWKIRIQDTVADNASCGVFTLGGTRRSPRDLDLAL
CCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCCCCCCCHHHEE
AGMVLEKNGEIVSTSCGASVQGSPVNAVAWLANTLGRFGIALKAGDVILSGSQSPLVPVK
EEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEECCEEEECCCCCEEEEC
PGDSLYCSVGGLGGTSVRFI
CCCCEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]