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The map label for this gene is hipO [H]

Identifier: 73539040

GI number: 73539040

Start: 1964511

End: 1965788

Strand: Direct

Name: hipO [H]

Synonym: Reut_B5217

Alternate gene names: 73539040

Gene position: 1964511-1965788 (Clockwise)

Preceding gene: 73539033

Following gene: 73539042

Centisome position: 72.06

GC content: 60.09

Gene sequence:

>1278_bases
ATGAACGCACTCGTCCAAGCTATGGCGACCCGCAGCGACACGTTGGTGACGGAACTCGAAGCCATCTACAAGGACTTTCA
CCAGCACCCCGAGTTGTCGATGCAGGAGTTCCGCACAGCCAAAGTCGCGGGAGATTACATCCAGGCCTTGGGCTATGAAG
TGACGCGTGAAGTCGGGGTGACCGGGCTTGTTGGTGTGCTGCGCAACGGCGAGGGACCCACGGTAATGCTCCGTGCAGAC
ATGGATGCTTTACCCATGGCCGAAAACACCGGCTTGTCCTATGCCAGCACCGTCAAGGCCAGAGATGAAGACGGCGTCGA
AGTCGATGTCGCGCATTCGTGTGGGCACGACATGCACGTGACATGGCTGATGGGGGTGGCGCGTGTCCTGGCCGAGCACC
GTGATGCCTGGAAGGGCACCGTCATGCTGGTCTTTCAACCAGGCGAAGAAGTGGGACGCGGTGCCAGCAGCATGGTCAAC
GCCTGGCATGCGGAACGCTTTCCCAAGCCAGACATCATTCTGGGCCAGCACGTCATGGTAGGCCCGAGCGGCACCGTCAG
TTATCGCCCGGGCGTCATTCTGTCAGCGGGCGATAGCCTTAAGATCAGGCTGTTCGGTCGTGGCTCGCATGGTTCGCAGC
CGCAGACTTCCATCGACCCCGTCATCATGGCCGCGTCCACGACGCTGCGCCTGCAGACCATCGTTTCGCGCGAGATTTCG
CCGATGGACAACGCGGTGCTGACGATTGGCTCACTGCAGGCAGGTACCAAGGAAAACATCATTCCGGACGAGGCAACCAT
CAAATTGAATATGCGCACGTTCGACGAGGACGTGCGCGAGTACATGCTGGCGTCCATCCGACGCATCTGTTGCGCGGAGT
GCGATGCATCCAACGCTGAGCGACCGCCGGAGTTCACCACGCTGAGCACGTATCCGCTGACGGAGAACGATATCGCAGCC
ACAAGTCAGGTGGCCGACGTCTTCAAGGCGCAGTTTGGCGATAAGGCCTGCGAGGCGCGGCCGGCCTCGGCCAGTGAGGA
CTTTAGCGTTTTTGGGCGAGCCTGGAACGTGCCCTACGTGTTCTGGTTCATCGGTGGCACCGACCCGGAAGTCTATGCAC
AGGCCAAAGCCGCAAAGCAGTTGAATAGGATACCTAGCAATCATTCGCCGAAGTTCGCACCCGTGATTCACCCCACATTG
CAAACGGGCCTGCAAGCGATGCTGACGGCGGCATCGGCGTGGCTTTGTCAGGCTCCCGAGTCGCGTGATGGAACATGA

Upstream 100 bases:

>100_bases
AAGCGTTGCAACAGTCCATTCAAGGAAGAGCCAAGGATTTCTTCCTGGCCGAAGCAACCCGGATAACGCAAACTGAACAC
TGAACGTCCGGAGGTGTTCC

Downstream 100 bases:

>100_bases
TGTCAGCCCGATCCTAGCGCGGGCCCGCCGCGCCTGACCGCTTCGCTTCTCGCCCCGGCGCGCCGCGACTTGCCTGCGCC
GCCCGGCATACCGGCGGATC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNALVQAMATRSDTLVTELEAIYKDFHQHPELSMQEFRTAKVAGDYIQALGYEVTREVGVTGLVGVLRNGEGPTVMLRAD
MDALPMAENTGLSYASTVKARDEDGVEVDVAHSCGHDMHVTWLMGVARVLAEHRDAWKGTVMLVFQPGEEVGRGASSMVN
AWHAERFPKPDIILGQHVMVGPSGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
PMDNAVLTIGSLQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIRRICCAECDASNAERPPEFTTLSTYPLTENDIAA
TSQVADVFKAQFGDKACEARPASASEDFSVFGRAWNVPYVFWFIGGTDPEVYAQAKAAKQLNRIPSNHSPKFAPVIHPTL
QTGLQAMLTAASAWLCQAPESRDGT

Sequences:

>Translated_425_residues
MNALVQAMATRSDTLVTELEAIYKDFHQHPELSMQEFRTAKVAGDYIQALGYEVTREVGVTGLVGVLRNGEGPTVMLRAD
MDALPMAENTGLSYASTVKARDEDGVEVDVAHSCGHDMHVTWLMGVARVLAEHRDAWKGTVMLVFQPGEEVGRGASSMVN
AWHAERFPKPDIILGQHVMVGPSGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
PMDNAVLTIGSLQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIRRICCAECDASNAERPPEFTTLSTYPLTENDIAA
TSQVADVFKAQFGDKACEARPASASEDFSVFGRAWNVPYVFWFIGGTDPEVYAQAKAAKQLNRIPSNHSPKFAPVIHPTL
QTGLQAMLTAASAWLCQAPESRDGT
>Mature_425_residues
MNALVQAMATRSDTLVTELEAIYKDFHQHPELSMQEFRTAKVAGDYIQALGYEVTREVGVTGLVGVLRNGEGPTVMLRAD
MDALPMAENTGLSYASTVKARDEDGVEVDVAHSCGHDMHVTWLMGVARVLAEHRDAWKGTVMLVFQPGEEVGRGASSMVN
AWHAERFPKPDIILGQHVMVGPSGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
PMDNAVLTIGSLQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIRRICCAECDASNAERPPEFTTLSTYPLTENDIAA
TSQVADVFKAQFGDKACEARPASASEDFSVFGRAWNVPYVFWFIGGTDPEVYAQAKAAKQLNRIPSNHSPKFAPVIHPTL
QTGLQAMLTAASAWLCQAPESRDGT

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=316, Percent_Identity=26.8987341772152, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 46202; Mature: 46202

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALVQAMATRSDTLVTELEAIYKDFHQHPELSMQEFRTAKVAGDYIQALGYEVTREVGV
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
TGLVGVLRNGEGPTVMLRADMDALPMAENTGLSYASTVKARDEDGVEVDVAHSCGHDMHV
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHEECCCCCCCCEEEECCCCCCCCHH
TWLMGVARVLAEHRDAWKGTVMLVFQPGEEVGRGASSMVNAWHAERFPKPDIILGQHVMV
HHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHCCCCCCEEECCEEEE
GPSGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
CCCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCC
PMDNAVLTIGSLQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIRRICCAECDASNAE
CCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPPEFTTLSTYPLTENDIAATSQVADVFKAQFGDKACEARPASASEDFSVFGRAWNVPYV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHEECCCCEE
FWFIGGTDPEVYAQAKAAKQLNRIPSNHSPKFAPVIHPTLQTGLQAMLTAASAWLCQAPE
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCC
SRDGT
CCCCC
>Mature Secondary Structure
MNALVQAMATRSDTLVTELEAIYKDFHQHPELSMQEFRTAKVAGDYIQALGYEVTREVGV
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
TGLVGVLRNGEGPTVMLRADMDALPMAENTGLSYASTVKARDEDGVEVDVAHSCGHDMHV
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHEECCCCCCCCEEEECCCCCCCCHH
TWLMGVARVLAEHRDAWKGTVMLVFQPGEEVGRGASSMVNAWHAERFPKPDIILGQHVMV
HHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHCCCCCCEEECCEEEE
GPSGTVSYRPGVILSAGDSLKIRLFGRGSHGSQPQTSIDPVIMAASTTLRLQTIVSREIS
CCCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCC
PMDNAVLTIGSLQAGTKENIIPDEATIKLNMRTFDEDVREYMLASIRRICCAECDASNAE
CCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPPEFTTLSTYPLTENDIAATSQVADVFKAQFGDKACEARPASASEDFSVFGRAWNVPYV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHEECCCCEE
FWFIGGTDPEVYAQAKAAKQLNRIPSNHSPKFAPVIHPTLQTGLQAMLTAASAWLCQAPE
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCC
SRDGT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]