Click here to switch to the map view.

The map label for this gene is livJ [C]

Identifier: 73538633

GI number: 73538633

Start: 1507517

End: 1508665

Strand: Reverse

Name: livJ [C]

Synonym: Reut_B4808

Alternate gene names: 73538633

Gene position: 1508665-1507517 (Counterclockwise)

Preceding gene: 73538634

Following gene: 73538632

Centisome position: 55.34

GC content: 59.97

Gene sequence:

>1149_bases
ATGATCCACTTTCTTCGACGGCTGCTCGTTACGAGCCTGAGCACACTCTTGCTGTCCGTCGTCTGCGGCCAGTCATATGG
GCAGATGGTCGTCAAGATCGGGGTTTCCAGTCCGCTTACTGGGATCAATGCCGCCAATGGCAAGGACATCGAGAACGGTG
TTCGTCTCGCTGTCGAGGAAGCCAACGCACAGCGCATCAGGCTGGATGGCCAGGAAGCCAGATTCCAGCTTGATTCCATG
GACGACCAAAGTGATCCTCGCATCGGCGTTCAGGTCGCCCAAAAGCTGGTCGACGACGGTGTCGCGCTCGTGGTCGGGTA
CTACAACTCCGGCGTTGCGCTTCCCTCGTCGCTTGTCTTCGCCAGGGCAGGCATTCCGGTCATCCTTCCTGCTGCCAGCA
ATCCGGCGATTACGCGCCAAGGCCTGAAGACGGTCTTCCGTGTCATTGCGACCGATACGCAAAACTCCGGTACGGCTGGG
AAGTATGCGGTCAACGACATGAAAGTCCAGCGCATTGCCGTGATGGACGACCGCACCGCGTTCGGGCAGGGGGCGGTGGA
TGAGTTCAGGAAGGCGGTCATCGGCGCCGGCGGAAAAATCGTGTTGGCGGAATACACCAACGACAAGGTGGTCGACTTCA
GCGCGCCGCTCACGAATATCCGGGCCGCCAACGCCGACCTCCTGTTCTTTGCCGGCCTGGATCACCAAGCCGCGCTCATT
ACGAAGCGGATGAGAGAGCTCGGCATGCGCACACAGTTCGTCGGCAGTGGCGGCATCGCCGACAGTATCTTCCTGAACGT
TGCCGGTCCGGCAGGGGAGGGCGCGATGGCGTGGGAATACGGTCGACCAGTCGAATCGCTGCCGCAAGGCAAGGTATTCA
TGCAGAAGTTCAGGAAGCGCTTCGGAAGCGACAGCCTCATCTATGCCCCTTTCGCGTATGACGCTACGTGGGCGGCTATA
AACGCGATCAGGCAGGCCAATTCCGCGACATCGGCCGAAATCGTATCCGCGCTGCGCGCGACGCAATACGAGGGCATCAC
CGGGAAGATTGCCTTCGATCCGAACGGCGACCTGAAGAATCCGATGTCGACGCTTTATCAGGTAAGAGGTGGGCGTTGGG
TTCCGGTAACGACGATCGGGGCCGATTGA

Upstream 100 bases:

>100_bases
GTGCCTGGCAGAGTAGAGGTCGCCGCCAGGGGCCCATTAGAATGAGAAGTATGTGGGCGGCAACGATGGCTCCGCCGTCC
TGAGTCGCCGGAGTTGCCTA

Downstream 100 bases:

>100_bases
TGTGACGATATTCAAGCGAATCCCGCCATGGCGTAAAGATCCCATCGTGACGACGCATCAGTTCTATCTCGCGGTCTCAA
GGATCTGATGTACTAGGGTC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MIHFLRRLLVTSLSTLLLSVVCGQSYGQMVVKIGVSSPLTGINAANGKDIENGVRLAVEEANAQRIRLDGQEARFQLDSM
DDQSDPRIGVQVAQKLVDDGVALVVGYYNSGVALPSSLVFARAGIPVILPAASNPAITRQGLKTVFRVIATDTQNSGTAG
KYAVNDMKVQRIAVMDDRTAFGQGAVDEFRKAVIGAGGKIVLAEYTNDKVVDFSAPLTNIRAANADLLFFAGLDHQAALI
TKRMRELGMRTQFVGSGGIADSIFLNVAGPAGEGAMAWEYGRPVESLPQGKVFMQKFRKRFGSDSLIYAPFAYDATWAAI
NAIRQANSATSAEIVSALRATQYEGITGKIAFDPNGDLKNPMSTLYQVRGGRWVPVTTIGAD

Sequences:

>Translated_382_residues
MIHFLRRLLVTSLSTLLLSVVCGQSYGQMVVKIGVSSPLTGINAANGKDIENGVRLAVEEANAQRIRLDGQEARFQLDSM
DDQSDPRIGVQVAQKLVDDGVALVVGYYNSGVALPSSLVFARAGIPVILPAASNPAITRQGLKTVFRVIATDTQNSGTAG
KYAVNDMKVQRIAVMDDRTAFGQGAVDEFRKAVIGAGGKIVLAEYTNDKVVDFSAPLTNIRAANADLLFFAGLDHQAALI
TKRMRELGMRTQFVGSGGIADSIFLNVAGPAGEGAMAWEYGRPVESLPQGKVFMQKFRKRFGSDSLIYAPFAYDATWAAI
NAIRQANSATSAEIVSALRATQYEGITGKIAFDPNGDLKNPMSTLYQVRGGRWVPVTTIGAD
>Mature_382_residues
MIHFLRRLLVTSLSTLLLSVVCGQSYGQMVVKIGVSSPLTGINAANGKDIENGVRLAVEEANAQRIRLDGQEARFQLDSM
DDQSDPRIGVQVAQKLVDDGVALVVGYYNSGVALPSSLVFARAGIPVILPAASNPAITRQGLKTVFRVIATDTQNSGTAG
KYAVNDMKVQRIAVMDDRTAFGQGAVDEFRKAVIGAGGKIVLAEYTNDKVVDFSAPLTNIRAANADLLFFAGLDHQAALI
TKRMRELGMRTQFVGSGGIADSIFLNVAGPAGEGAMAWEYGRPVESLPQGKVFMQKFRKRFGSDSLIYAPFAYDATWAAI
NAIRQANSATSAEIVSALRATQYEGITGKIAFDPNGDLKNPMSTLYQVRGGRWVPVTTIGAD

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=334, Percent_Identity=29.0419161676647, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI48994941, Length=333, Percent_Identity=27.6276276276276, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40868; Mature: 40868

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHFLRRLLVTSLSTLLLSVVCGQSYGQMVVKIGVSSPLTGINAANGKDIENGVRLAVEE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEC
ANAQRIRLDGQEARFQLDSMDDQSDPRIGVQVAQKLVDDGVALVVGYYNSGVALPSSLVF
CCCEEEEECCHHHEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHH
ARAGIPVILPAASNPAITRQGLKTVFRVIATDTQNSGTAGKYAVNDMKVQRIAVMDDRTA
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEEEECCCCC
FGQGAVDEFRKAVIGAGGKIVLAEYTNDKVVDFSAPLTNIRAANADLLFFAGLDHQAALI
CCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHH
TKRMRELGMRTQFVGSGGIADSIFLNVAGPAGEGAMAWEYGRPVESLPQGKVFMQKFRKR
HHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCCEEECCCCCHHHCCCCHHHHHHHHHH
FGSDSLIYAPFAYDATWAAINAIRQANSATSAEIVSALRATQYEGITGKIAFDPNGDLKN
HCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
PMSTLYQVRGGRWVPVTTIGAD
HHHHHHHHCCCEEEEEEECCCC
>Mature Secondary Structure
MIHFLRRLLVTSLSTLLLSVVCGQSYGQMVVKIGVSSPLTGINAANGKDIENGVRLAVEE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEC
ANAQRIRLDGQEARFQLDSMDDQSDPRIGVQVAQKLVDDGVALVVGYYNSGVALPSSLVF
CCCEEEEECCHHHEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHH
ARAGIPVILPAASNPAITRQGLKTVFRVIATDTQNSGTAGKYAVNDMKVQRIAVMDDRTA
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEEEECCCCC
FGQGAVDEFRKAVIGAGGKIVLAEYTNDKVVDFSAPLTNIRAANADLLFFAGLDHQAALI
CCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHH
TKRMRELGMRTQFVGSGGIADSIFLNVAGPAGEGAMAWEYGRPVESLPQGKVFMQKFRKR
HHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCCEEECCCCCHHHCCCCHHHHHHHHHH
FGSDSLIYAPFAYDATWAAINAIRQANSATSAEIVSALRATQYEGITGKIAFDPNGDLKN
HCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
PMSTLYQVRGGRWVPVTTIGAD
HHHHHHHHCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA