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The map label for this gene is est [H]

Identifier: 73538166

GI number: 73538166

Start: 996094

End: 997068

Strand: Reverse

Name: est [H]

Synonym: Reut_B4336

Alternate gene names: 73538166

Gene position: 997068-996094 (Counterclockwise)

Preceding gene: 73538169

Following gene: 73538165

Centisome position: 36.57

GC content: 69.54

Gene sequence:

>975_bases
ATGATAGGCGTGGCAGCGCATTTCCGCACGCCCGCCAAGGTGCCCATGTCCCAGTCGCCCAGCCATCGCCCCCCCGCCGC
GCCGCCGGCCACCGGCAACGAGCAGGCGCGCGTCGCGGAATTCAGCGTGGCAAGCGGCGAGCGCGAGATATCGGTATGCG
TGTGCTATCCGCCCGGATATCCAGCACCGGCCACTGAAAGTGGTGCGCTGCTGCCGTGGTTGGTGTACCTGCACGCGGGT
GGTTTCGTGGACGGCGGACTGGAACGGGCCAAGGAGGTCGTGCAGATGCTGGCGGCCTCGGTTCCGGCGGTGGTGGTGAC
GCCAGCTTATTCGCTGGCGCCGGACCATCCGTTTCCAGCCGCGCCGGAGGATGCGTACAACACCGTGGAATGGGTGCTGC
GCTACGCGCGCCGCCTGAAGGTGGACAAGGCACGCTTTGCGCTGGTTGGCGAAGAGGCGGGCGGCAACCTGGCGATCGCG
CTGTCGCAGATGCTGCGCGACCGTTGCGCCCCGCAACCGAGCGGGCAATGGCTGATCCGCCCGGTAACGGACCCGTGCCT
GCAGCATGCGTCGTGCACCGGCGCGGGCCGTGGCGCGGTACCGCTGGAAACCCTGCAGCGGCTCGCCTGCAATTATCGCG
ACTATCTGCCGACGCCGGCCGCCAGCGTGCATCCGTATGCCGCGCCGGCGCTGGCCTCGCGGCTGGCAGGGCTGCCACCC
ACGCTGGTGCAGGTGGCGGAAGTCGATGCGCTGCGCGCCGAAGGCGAAGCCTTCGCGGGGCGCCTTGCCAAGGCCGGTGT
GCCGGCCGAGGCCATGATCATGCCTGGCGCCTGTGGCGACGGCGTAGAAGACACCCACGACGACTGTCAGTCGTGGGTAG
ACGAAGGTGCGCGGTTCCTGCGGCGATGTCTGGCCGGGGCCGACGACACCTCATCCCGGCGCGAAAGCCGGTCCGGAATG
AAGCCTGCCGGCTGA

Upstream 100 bases:

>100_bases
GTCTCAGCGGGCCGATTATTCCAATCGCGGAACAGTGCGTTGGCAATCTCTCGCTTTATCCCATCGGCGTCAATCACCAT
AATCCGTTGCATCGCAACAC

Downstream 100 bases:

>100_bases
GGCATTCCGGGTAACACGCCAGCCGGTACTGATGCCGCCGAGACGGACTGCGCGGCATCCGGCAAACAGACTTGCTGATC
TGTCCGACAAGCTCCGGACC

Product: esterase/lipase/thioesterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MIGVAAHFRTPAKVPMSQSPSHRPPAAPPATGNEQARVAEFSVASGEREISVCVCYPPGYPAPATESGALLPWLVYLHAG
GFVDGGLERAKEVVQMLAASVPAVVVTPAYSLAPDHPFPAAPEDAYNTVEWVLRYARRLKVDKARFALVGEEAGGNLAIA
LSQMLRDRCAPQPSGQWLIRPVTDPCLQHASCTGAGRGAVPLETLQRLACNYRDYLPTPAASVHPYAAPALASRLAGLPP
TLVQVAEVDALRAEGEAFAGRLAKAGVPAEAMIMPGACGDGVEDTHDDCQSWVDEGARFLRRCLAGADDTSSRRESRSGM
KPAG

Sequences:

>Translated_324_residues
MIGVAAHFRTPAKVPMSQSPSHRPPAAPPATGNEQARVAEFSVASGEREISVCVCYPPGYPAPATESGALLPWLVYLHAG
GFVDGGLERAKEVVQMLAASVPAVVVTPAYSLAPDHPFPAAPEDAYNTVEWVLRYARRLKVDKARFALVGEEAGGNLAIA
LSQMLRDRCAPQPSGQWLIRPVTDPCLQHASCTGAGRGAVPLETLQRLACNYRDYLPTPAASVHPYAAPALASRLAGLPP
TLVQVAEVDALRAEGEAFAGRLAKAGVPAEAMIMPGACGDGVEDTHDDCQSWVDEGARFLRRCLAGADDTSSRRESRSGM
KPAG
>Mature_324_residues
MIGVAAHFRTPAKVPMSQSPSHRPPAAPPATGNEQARVAEFSVASGEREISVCVCYPPGYPAPATESGALLPWLVYLHAG
GFVDGGLERAKEVVQMLAASVPAVVVTPAYSLAPDHPFPAAPEDAYNTVEWVLRYARRLKVDKARFALVGEEAGGNLAIA
LSQMLRDRCAPQPSGQWLIRPVTDPCLQHASCTGAGRGAVPLETLQRLACNYRDYLPTPAASVHPYAAPALASRLAGLPP
TLVQVAEVDALRAEGEAFAGRLAKAGVPAEAMIMPGACGDGVEDTHDDCQSWVDEGARFLRRCLAGADDTSSRRESRSGM
KPAG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=303, Percent_Identity=29.042904290429, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34155; Mature: 34155

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVAAHFRTPAKVPMSQSPSHRPPAAPPATGNEQARVAEFSVASGEREISVCVCYPPGY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCEEEEEEECCCCC
PAPATESGALLPWLVYLHAGGFVDGGLERAKEVVQMLAASVPAVVVTPAYSLAPDHPFPA
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCCC
APEDAYNTVEWVLRYARRLKVDKARFALVGEEAGGNLAIALSQMLRDRCAPQPSGQWLIR
CCHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEE
PVTDPCLQHASCTGAGRGAVPLETLQRLACNYRDYLPTPAASVHPYAAPALASRLAGLPP
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCH
TLVQVAEVDALRAEGEAFAGRLAKAGVPAEAMIMPGACGDGVEDTHDDCQSWVDEGARFL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RRCLAGADDTSSRRESRSGMKPAG
HHHHCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MIGVAAHFRTPAKVPMSQSPSHRPPAAPPATGNEQARVAEFSVASGEREISVCVCYPPGY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCEEEEEEECCCCC
PAPATESGALLPWLVYLHAGGFVDGGLERAKEVVQMLAASVPAVVVTPAYSLAPDHPFPA
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHCCCCCCCCC
APEDAYNTVEWVLRYARRLKVDKARFALVGEEAGGNLAIALSQMLRDRCAPQPSGQWLIR
CCHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEE
PVTDPCLQHASCTGAGRGAVPLETLQRLACNYRDYLPTPAASVHPYAAPALASRLAGLPP
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCH
TLVQVAEVDALRAEGEAFAGRLAKAGVPAEAMIMPGACGDGVEDTHDDCQSWVDEGARFL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RRCLAGADDTSSRRESRSGMKPAG
HHHHCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]