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The map label for this gene is caiD [H]

Identifier: 73537543

GI number: 73537543

Start: 295783

End: 296469

Strand: Direct

Name: caiD [H]

Synonym: Reut_B3708

Alternate gene names: 73537543

Gene position: 295783-296469 (Clockwise)

Preceding gene: 73537542

Following gene: 73537544

Centisome position: 10.85

GC content: 69.14

Gene sequence:

>687_bases
ATGAGTGAAGCGGCAATAACCTCACCGGTACTGCTGGAGCGCCATGCCGGCTGGACCGAGATCGTGTTGAACCGGCCCGA
GCGCCGCAATGCCATCGACATTGCGCTAGCCAGCGCGCTGGCCACCGCCATCGACAGCGTCAACGCCGACGACAGCACGC
GTGCCGTGCTGCTGCGCGGCGCCGATGGCGCGTTCTGTTCGGGACTGGATCTGAAGGCGCTGCAGGCCGAGCCGGACGGC
ATGGCGAAATTCGCGCAGGTCTGGGAGCGTGTCCACCTGTCGCTGCTACAGAGCGAGAAGGCGTGGATCGTGGCGCTGGA
GCGCCATGCGGTCAACGGCGGCGCGGCACTGGCTCTGGCCGGCGACCTGATGGTGTGCGGCGCCGGCGCCTTTCTGCAGA
TCGGCGAGATCCGCCTTGGCATGGCCGCGCCGCGCAATGCGGCATGGTTGGCACTGCGCCATTCCGAAGCGGTCGCCGCG
CGGCTGTGCCTGCTTGGCGATCGCGTACCCGCCGCTGACCTGCTTCGGCTTGGCGTGGCCACGGAAGTCACCGACGACAC
GCAGGTGCTCGACCGCGCGCGCGACCTGGCCACGACCATTGCCGCTTTCCCGGCCGGCGGCGTGACCGCGATCAAGCGTG
GCATGCGTGCCGCCAGCACGCGCATGGCGCGCGATACCCACGACTGA

Upstream 100 bases:

>100_bases
AGCACACCACTGAAGTGCTGACCGAGCTTGGCTACGGTGCCACTGAAATTGATGAACTGGCCTGCGCCGGCGCCATTGGC
TGCGAGCAAGGAGTGACTGC

Downstream 100 bases:

>100_bases
AGCGAGATCCCGCATGAGTGAATCCATCACGACTGACAGCGAAGCCTCCCTGGAGGCATTCCGCCGCGAGGTCCGCGACT
TTGTTGCGCAGGCCTTGCCG

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSEAAITSPVLLERHAGWTEIVLNRPERRNAIDIALASALATAIDSVNADDSTRAVLLRGADGAFCSGLDLKALQAEPDG
MAKFAQVWERVHLSLLQSEKAWIVALERHAVNGGAALALAGDLMVCGAGAFLQIGEIRLGMAAPRNAAWLALRHSEAVAA
RLCLLGDRVPAADLLRLGVATEVTDDTQVLDRARDLATTIAAFPAGGVTAIKRGMRAASTRMARDTHD

Sequences:

>Translated_228_residues
MSEAAITSPVLLERHAGWTEIVLNRPERRNAIDIALASALATAIDSVNADDSTRAVLLRGADGAFCSGLDLKALQAEPDG
MAKFAQVWERVHLSLLQSEKAWIVALERHAVNGGAALALAGDLMVCGAGAFLQIGEIRLGMAAPRNAAWLALRHSEAVAA
RLCLLGDRVPAADLLRLGVATEVTDDTQVLDRARDLATTIAAFPAGGVTAIKRGMRAASTRMARDTHD
>Mature_227_residues
SEAAITSPVLLERHAGWTEIVLNRPERRNAIDIALASALATAIDSVNADDSTRAVLLRGADGAFCSGLDLKALQAEPDGM
AKFAQVWERVHLSLLQSEKAWIVALERHAVNGGAALALAGDLMVCGAGAFLQIGEIRLGMAAPRNAAWLALRHSEAVAAR
LCLLGDRVPAADLLRLGVATEVTDDTQVLDRARDLATTIAAFPAGGVTAIKRGMRAASTRMARDTHD

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=186, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=24.4791666666667, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI37594471, Length=192, Percent_Identity=24.4791666666667, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=217, Percent_Identity=31.7972350230415, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI221142681, Length=224, Percent_Identity=29.9107142857143, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787659, Length=214, Percent_Identity=26.6355140186916, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=205, Percent_Identity=31.219512195122, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650670, Length=210, Percent_Identity=32.8571428571429, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357171, Length=210, Percent_Identity=29.5238095238095, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20129971, Length=184, Percent_Identity=28.804347826087, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653477, Length=184, Percent_Identity=28.804347826087, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 24050; Mature: 23919

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAAITSPVLLERHAGWTEIVLNRPERRNAIDIALASALATAIDSVNADDSTRAVLLRG
CCCCCCCCCHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
ADGAFCSGLDLKALQAEPDGMAKFAQVWERVHLSLLQSEKAWIVALERHAVNGGAALALA
CCCCEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEE
GDLMVCGAGAFLQIGEIRLGMAAPRNAAWLALRHSEAVAARLCLLGDRVPAADLLRLGVA
CCEEEECCCCEEEECCEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHCCH
TEVTDDTQVLDRARDLATTIAAFPAGGVTAIKRGMRAASTRMARDTHD
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEAAITSPVLLERHAGWTEIVLNRPERRNAIDIALASALATAIDSVNADDSTRAVLLRG
CCCCCCCCHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
ADGAFCSGLDLKALQAEPDGMAKFAQVWERVHLSLLQSEKAWIVALERHAVNGGAALALA
CCCCEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEE
GDLMVCGAGAFLQIGEIRLGMAAPRNAAWLALRHSEAVAARLCLLGDRVPAADLLRLGVA
CCEEEECCCCEEEECCEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHCCH
TEVTDDTQVLDRARDLATTIAAFPAGGVTAIKRGMRAASTRMARDTHD
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA