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The map label for this gene is metE

Identifier: 71892393

GI number: 71892393

Start: 781518

End: 783812

Strand: Reverse

Name: metE

Synonym: BPEN_652

Alternate gene names: 71892393

Gene position: 783812-781518 (Counterclockwise)

Preceding gene: 71892394

Following gene: 71892392

Centisome position: 99.01

GC content: 31.29

Gene sequence:

>2295_bases
ATGACAATTGTCGGTCATATATTAGGATTTCCTCGTATTGGATTACATAGAGAATTAAAATATGCTCTAGAATATTATTG
GGATGGAAAAATCAAAGAAGATAAATTATTAGAAATAGGCCGTACATTACGCATACGTCATTGGAAACAACAGAAAGATT
CCGGTATCGACTGGATTTCAGTAGGTGATTTCGCATGGTACGATCATGTTTTAACTACTAGCTTGATGCTCAATAATATT
CCAGATAGGCATCGTATTGGTCAAGAAAATATTAATTTAAATACATTGTTTAGAATAAGCCGAGGGTATTCTTTAGACAA
AAAATTGATACCTCCTTCAGAAATGAAAAAATGGTTTAATACAAATTATCACTATATAGTACCCGAATTTGTTCCCAATC
AGGAATTTAAGTTAAATTGGACTCAATTATTTGATGAAATAGATGAAGCTTTGTCTCTAAAATATACGGTAAAACCAATA
CTACTTGGCCCAATTAGTTATCTTTGGTTAGGAAAAATAAAAGGAAAAAAATTTGATCGTTTATCATTATTACCCAATTT
ATTATCAGTATATCAAGAAATATTAGATATTTTATTATCAAAAAATATTGATTGGATACAAATTGATGAGCCCATACTAG
TTTTGGAGCTACCGTTACAATGGCTAAATGCATATTTAAGCGCGTATGAACAACTTCATGGTAAAGTTAAATTACTATTA
ACTACTTATTTCGATAGCATATATCATCAATTAGATATTATTACGCGATTAAAAATAGATGGGCTACATGTAGATCTGGT
TTCTAATCCTGATAATATACCTTTACTGCATGCACGACTACCAAAACATTGGACATTATCAGCAGGAGTAGTTAATGGAC
GTAATGTATGGAAAACTAATTTACATGATTGGTTTAAAAAACTATATCCTTTAGTAGGCGAGCGCAGCTTATGGATAGGT
TCTTCTTGTTCCTTATTACACAGTCCTATTGATCTAAATACGGAGATAAAGTTAGATAAACACATTAAAGAATGGTTTGC
TTTTGCGTTGCAAAAATGTTACGAAATAAAAATGTTATGCGCTGCATTGAATGCTGAAAACACAAGTGACGCGACATATG
AAGATACACTTAGTGAATATTATATTTCTAATAACTTACGATCGTATTCTAGTACAATTCATAATATACAAGTTAAAAAA
CGTCTCGAACACATCGATGAAATTTCTTATTCTAGATCTTCTCCGTACACAACACGAGTTACATTACAGCATAAGAAGTT
GAATTTACCATTATTTCCAACAACTACTATCGGTTCATTTCCTCAAACTGAAGAAATTCGTAAACTTCGTTCGCGCTTTA
AAAATAACCAAATCAACAAAGAAGATTATCATCTTAATATTAAGAATTACATTAAAAAAATTATTATGGAACAAGAAAAA
TTAGATTTAGATATTCTAGTGCATGGAGAACCAGAACGAAATGATATGGTGGAATATTTTGGAGAGAATTTAAATGGATT
TATATTTACTCAAAATGGGTGGGTACAAAGTTATGGTTCACGTTGTGTAAAACCTCCGGTAATTATTGGGGATATTAGTC
GTTCAAAACCCATTACTATAGAATGGATAAGTTATGCTCAGTCATTAACTAATAAACCGATAAAAGGTATATTAACTGGA
CCTGTCACCATAATGTCTTGGTCGTTCCCACGAGAGGATATGCAACGAAAAACAATAGCATTGCAATTAGCGTTATCGAT
ACGTGATGAAGTAATAGATTTGGAAAAATCTGGTATTGGAATCATACAAATTGATGAGCCAGCTTTGCGAGAAGGATTAC
CTTTAAAACGCTCTGATCAAAAACAATATTTAGAATGGGCAATTAATGTCTTTAGGATCACTGTATCATCAGTGAAAGAT
GACACTCAAATTCATACTCATATGTGTTATTCTGAATTTAGTGATATCATTTATGCTATTCTAGCATTAGATGCGGATGT
CATCAGTATAGAAGCAGCTAGGTCAGATGAGAAAATATTAAAATCCATACGACATGTAATAAAGAATTTAAATGAAATTG
GACCAGGAATATACGATATTCATTCTCCAAATAAACCAACTCAAAATGATCTTATCAGAAGAATTAATCAATTGTTAAAA
TATATCCCAGAACAACGTTTATGGATTAATCCTGATTGTGGATTAAAAACAAGATCATGGCCTGAAATTAAACATTCGTT
GAATAACATGGTGTCTGCAGCTAAATTATTGCGTAAACAATTTTATTCATCGTAA

Upstream 100 bases:

>100_bases
ATAATCGAATTAACTATTGGTGCCATAATACTTTTTTAAATTTTTTACTATAATTTTTTATGAGTACTTCATATAATAAA
AATAGCTCATGGAGTGAATT

Downstream 100 bases:

>100_bases
ATAATGACTAATAATTTTTATTAATTGATTGTATAGCAATCAATATCAAGATAAAATTACCGGATTTATTGGTAATACAA
TGGATTGATTACGAAATCAT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MTIVGHILGFPRIGLHRELKYALEYYWDGKIKEDKLLEIGRTLRIRHWKQQKDSGIDWISVGDFAWYDHVLTTSLMLNNI
PDRHRIGQENINLNTLFRISRGYSLDKKLIPPSEMKKWFNTNYHYIVPEFVPNQEFKLNWTQLFDEIDEALSLKYTVKPI
LLGPISYLWLGKIKGKKFDRLSLLPNLLSVYQEILDILLSKNIDWIQIDEPILVLELPLQWLNAYLSAYEQLHGKVKLLL
TTYFDSIYHQLDIITRLKIDGLHVDLVSNPDNIPLLHARLPKHWTLSAGVVNGRNVWKTNLHDWFKKLYPLVGERSLWIG
SSCSLLHSPIDLNTEIKLDKHIKEWFAFALQKCYEIKMLCAALNAENTSDATYEDTLSEYYISNNLRSYSSTIHNIQVKK
RLEHIDEISYSRSSPYTTRVTLQHKKLNLPLFPTTTIGSFPQTEEIRKLRSRFKNNQINKEDYHLNIKNYIKKIIMEQEK
LDLDILVHGEPERNDMVEYFGENLNGFIFTQNGWVQSYGSRCVKPPVIIGDISRSKPITIEWISYAQSLTNKPIKGILTG
PVTIMSWSFPREDMQRKTIALQLALSIRDEVIDLEKSGIGIIQIDEPALREGLPLKRSDQKQYLEWAINVFRITVSSVKD
DTQIHTHMCYSEFSDIIYAILALDADVISIEAARSDEKILKSIRHVIKNLNEIGPGIYDIHSPNKPTQNDLIRRINQLLK
YIPEQRLWINPDCGLKTRSWPEIKHSLNNMVSAAKLLRKQFYSS

Sequences:

>Translated_764_residues
MTIVGHILGFPRIGLHRELKYALEYYWDGKIKEDKLLEIGRTLRIRHWKQQKDSGIDWISVGDFAWYDHVLTTSLMLNNI
PDRHRIGQENINLNTLFRISRGYSLDKKLIPPSEMKKWFNTNYHYIVPEFVPNQEFKLNWTQLFDEIDEALSLKYTVKPI
LLGPISYLWLGKIKGKKFDRLSLLPNLLSVYQEILDILLSKNIDWIQIDEPILVLELPLQWLNAYLSAYEQLHGKVKLLL
TTYFDSIYHQLDIITRLKIDGLHVDLVSNPDNIPLLHARLPKHWTLSAGVVNGRNVWKTNLHDWFKKLYPLVGERSLWIG
SSCSLLHSPIDLNTEIKLDKHIKEWFAFALQKCYEIKMLCAALNAENTSDATYEDTLSEYYISNNLRSYSSTIHNIQVKK
RLEHIDEISYSRSSPYTTRVTLQHKKLNLPLFPTTTIGSFPQTEEIRKLRSRFKNNQINKEDYHLNIKNYIKKIIMEQEK
LDLDILVHGEPERNDMVEYFGENLNGFIFTQNGWVQSYGSRCVKPPVIIGDISRSKPITIEWISYAQSLTNKPIKGILTG
PVTIMSWSFPREDMQRKTIALQLALSIRDEVIDLEKSGIGIIQIDEPALREGLPLKRSDQKQYLEWAINVFRITVSSVKD
DTQIHTHMCYSEFSDIIYAILALDADVISIEAARSDEKILKSIRHVIKNLNEIGPGIYDIHSPNKPTQNDLIRRINQLLK
YIPEQRLWINPDCGLKTRSWPEIKHSLNNMVSAAKLLRKQFYSS
>Mature_763_residues
TIVGHILGFPRIGLHRELKYALEYYWDGKIKEDKLLEIGRTLRIRHWKQQKDSGIDWISVGDFAWYDHVLTTSLMLNNIP
DRHRIGQENINLNTLFRISRGYSLDKKLIPPSEMKKWFNTNYHYIVPEFVPNQEFKLNWTQLFDEIDEALSLKYTVKPIL
LGPISYLWLGKIKGKKFDRLSLLPNLLSVYQEILDILLSKNIDWIQIDEPILVLELPLQWLNAYLSAYEQLHGKVKLLLT
TYFDSIYHQLDIITRLKIDGLHVDLVSNPDNIPLLHARLPKHWTLSAGVVNGRNVWKTNLHDWFKKLYPLVGERSLWIGS
SCSLLHSPIDLNTEIKLDKHIKEWFAFALQKCYEIKMLCAALNAENTSDATYEDTLSEYYISNNLRSYSSTIHNIQVKKR
LEHIDEISYSRSSPYTTRVTLQHKKLNLPLFPTTTIGSFPQTEEIRKLRSRFKNNQINKEDYHLNIKNYIKKIIMEQEKL
DLDILVHGEPERNDMVEYFGENLNGFIFTQNGWVQSYGSRCVKPPVIIGDISRSKPITIEWISYAQSLTNKPIKGILTGP
VTIMSWSFPREDMQRKTIALQLALSIRDEVIDLEKSGIGIIQIDEPALREGLPLKRSDQKQYLEWAINVFRITVSSVKDD
TQIHTHMCYSEFSDIIYAILALDADVISIEAARSDEKILKSIRHVIKNLNEIGPGIYDIHSPNKPTQNDLIRRINQLLKY
IPEQRLWINPDCGLKTRSWPEIKHSLNNMVSAAKLLRKQFYSS

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=759, Percent_Identity=58.7615283267457, Blast_Score=960, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=776, Percent_Identity=45.6185567010309, Blast_Score=676, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_BLOPB (Q491V4)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278127.1
- HSSP:   Q9X112
- ProteinModelPortal:   Q491V4
- SMR:   Q491V4
- STRING:   Q491V4
- GeneID:   3562729
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_652
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   CBLO291272:BPEN_652-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 89004; Mature: 88873

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVGHILGFPRIGLHRELKYALEYYWDGKIKEDKLLEIGRTLRIRHWKQQKDSGIDWIS
CEEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEE
VGDFAWYDHVLTTSLMLNNIPDRHRIGQENINLNTLFRISRGYSLDKKLIPPSEMKKWFN
ECCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHC
TNYHYIVPEFVPNQEFKLNWTQLFDEIDEALSLKYTVKPILLGPISYLWLGKIKGKKFDR
CCCEEECCCCCCCCCEEEEHHHHHHHHHHHHEEEEEECHHEECCHHHHHHCCCCCCCCHH
LSLLPNLLSVYQEILDILLSKNIDWIQIDEPILVLELPLQWLNAYLSAYEQLHGKVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHCCHHHHHH
TTYFDSIYHQLDIITRLKIDGLHVDLVSNPDNIPLLHARLPKHWTLSAGVVNGRNVWKTN
HHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCEECC
LHDWFKKLYPLVGERSLWIGSSCSLLHSPIDLNTEIKLDKHIKEWFAFALQKCYEIKMLC
HHHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
AALNAENTSDATYEDTLSEYYISNNLRSYSSTIHNIQVKKRLEHIDEISYSRSSPYTTRV
HHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
TLQHKKLNLPLFPTTTIGSFPQTEEIRKLRSRFKNNQINKEDYHLNIKNYIKKIIMEQEK
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHC
LDLDILVHGEPERNDMVEYFGENLNGFIFTQNGWVQSYGSRCVKPPVIIGDISRSKPITI
CCEEEEEECCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCEEEEECCCCCCEEE
EWISYAQSLTNKPIKGILTGPVTIMSWSFPREDMQRKTIALQLALSIRDEVIDLEKSGIG
HHHHHHHHHCCCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IIQIDEPALREGLPLKRSDQKQYLEWAINVFRITVSSVKDDTQIHTHMCYSEFSDIIYAI
EEEECCHHHHCCCCCCCCCHHHHHHHHHHHHEEEHHHCCCCHHHHHHHHHHHHHHHHHHH
LALDADVISIEAARSDEKILKSIRHVIKNLNEIGPGIYDIHSPNKPTQNDLIRRINQLLK
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHH
YIPEQRLWINPDCGLKTRSWPEIKHSLNNMVSAAKLLRKQFYSS
HCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIVGHILGFPRIGLHRELKYALEYYWDGKIKEDKLLEIGRTLRIRHWKQQKDSGIDWIS
EEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEE
VGDFAWYDHVLTTSLMLNNIPDRHRIGQENINLNTLFRISRGYSLDKKLIPPSEMKKWFN
ECCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHC
TNYHYIVPEFVPNQEFKLNWTQLFDEIDEALSLKYTVKPILLGPISYLWLGKIKGKKFDR
CCCEEECCCCCCCCCEEEEHHHHHHHHHHHHEEEEEECHHEECCHHHHHHCCCCCCCCHH
LSLLPNLLSVYQEILDILLSKNIDWIQIDEPILVLELPLQWLNAYLSAYEQLHGKVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHCCHHHHHH
TTYFDSIYHQLDIITRLKIDGLHVDLVSNPDNIPLLHARLPKHWTLSAGVVNGRNVWKTN
HHHHHHHHHHHHHHHEEEECCEEEEEECCCCCCCEEEECCCCCEEEECCCCCCCCCEECC
LHDWFKKLYPLVGERSLWIGSSCSLLHSPIDLNTEIKLDKHIKEWFAFALQKCYEIKMLC
HHHHHHHHHHHHCCCCEEECCCHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
AALNAENTSDATYEDTLSEYYISNNLRSYSSTIHNIQVKKRLEHIDEISYSRSSPYTTRV
HHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
TLQHKKLNLPLFPTTTIGSFPQTEEIRKLRSRFKNNQINKEDYHLNIKNYIKKIIMEQEK
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHC
LDLDILVHGEPERNDMVEYFGENLNGFIFTQNGWVQSYGSRCVKPPVIIGDISRSKPITI
CCEEEEEECCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCEEEEECCCCCCEEE
EWISYAQSLTNKPIKGILTGPVTIMSWSFPREDMQRKTIALQLALSIRDEVIDLEKSGIG
HHHHHHHHHCCCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IIQIDEPALREGLPLKRSDQKQYLEWAINVFRITVSSVKDDTQIHTHMCYSEFSDIIYAI
EEEECCHHHHCCCCCCCCCHHHHHHHHHHHHEEEHHHCCCCHHHHHHHHHHHHHHHHHHH
LALDADVISIEAARSDEKILKSIRHVIKNLNEIGPGIYDIHSPNKPTQNDLIRRINQLLK
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHH
YIPEQRLWINPDCGLKTRSWPEIKHSLNNMVSAAKLLRKQFYSS
HCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA