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The map label for this gene is rpoH [H]

Identifier: 71892394

GI number: 71892394

Start: 784116

End: 784964

Strand: Reverse

Name: rpoH [H]

Synonym: BPEN_653

Alternate gene names: 71892394

Gene position: 784964-784116 (Counterclockwise)

Preceding gene: 71892395

Following gene: 71892393

Centisome position: 99.15

GC content: 31.45

Gene sequence:

>849_bases
ATGCCTAAATATATACAAAAAAATGTAACTTTAATTCCTCAAGGGAATTTAGAAGCTTATATAAAAGTAGCCAATGCTTA
TACTATGTTAACAGCAGAAGAAGAAAAAACATTAGCTAAACGCTTATACTATAATGGTGATTTAGATGCTGCAAAAAAAC
TCATTGTATCACATTTAAGATTTGTTGTTCATGTTTCTCGTAATTATTCTGGTTATGGATTACCTCAGGCCGATTTAATA
CAAGAAGGTAATATTGGTTTAATGAAAGCTGTTCGTCGATTTAATCCAGAGTTAGATGTTCGTTTAGTATCATTTGCTGT
ACACTGGATTAAATCCGAAATTCATGAATATGTTTTAAAAAATTGGCGTATTGTAAAAATAGCTACCACTAAAGCGCAGC
GTAAATTATTTTTTAATCTACGCAAAACTAAACAACGTTTAGGTTGGTTTAATGCAGGAGAAATAGAAATAGTCGCAAAA
GCGTTAAATGTGACTAGCAAAGACGTGCGTGAAATGGAATCCCGAATGTCAGCGCAAGATATGACTTTTGAACAATCTTC
AGATGAAGATAATAGAGGAATAACAATAGCACCTATTTTATATTTACAAGACAAAATTGTTTCCTCTAATGAAGAAGAGG
AATGGATTGATCAAGTTAGTGATAAATTAAATGCAGCTATAAAGACTTTAGATGAACGCAGTCAACACATTATTCGTGCC
CGATGGTTAAATGTAGATAATAAAAGTACGTTACAAGAATTAGCCAATCAATACGGTGTATCTGCTGAACGAGTACGTCA
ATTAGAAAAAAATGCCATGAAAAAAATGAAACTAATTATACAAAATTAA

Upstream 100 bases:

>100_bases
TTGATACAAAATTTTTTACAATGATTCCGATGCAATTACATGCAGACGCAATGATGTACTCATAATTATCTGCGTATAAA
TGGTTATTTTAGGAGAATAA

Downstream 100 bases:

>100_bases
TTAAAATTTTTTGAATTTCATTGGTGAAACTAAATAATATAAAAATTGTTAAAATGTATATATACTTATTAATTTTTAAT
TGTTTGATTATAGTATATCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPKYIQKNVTLIPQGNLEAYIKVANAYTMLTAEEEKTLAKRLYYNGDLDAAKKLIVSHLRFVVHVSRNYSGYGLPQADLI
QEGNIGLMKAVRRFNPELDVRLVSFAVHWIKSEIHEYVLKNWRIVKIATTKAQRKLFFNLRKTKQRLGWFNAGEIEIVAK
ALNVTSKDVREMESRMSAQDMTFEQSSDEDNRGITIAPILYLQDKIVSSNEEEEWIDQVSDKLNAAIKTLDERSQHIIRA
RWLNVDNKSTLQELANQYGVSAERVRQLEKNAMKKMKLIIQN

Sequences:

>Translated_282_residues
MPKYIQKNVTLIPQGNLEAYIKVANAYTMLTAEEEKTLAKRLYYNGDLDAAKKLIVSHLRFVVHVSRNYSGYGLPQADLI
QEGNIGLMKAVRRFNPELDVRLVSFAVHWIKSEIHEYVLKNWRIVKIATTKAQRKLFFNLRKTKQRLGWFNAGEIEIVAK
ALNVTSKDVREMESRMSAQDMTFEQSSDEDNRGITIAPILYLQDKIVSSNEEEEWIDQVSDKLNAAIKTLDERSQHIIRA
RWLNVDNKSTLQELANQYGVSAERVRQLEKNAMKKMKLIIQN
>Mature_281_residues
PKYIQKNVTLIPQGNLEAYIKVANAYTMLTAEEEKTLAKRLYYNGDLDAAKKLIVSHLRFVVHVSRNYSGYGLPQADLIQ
EGNIGLMKAVRRFNPELDVRLVSFAVHWIKSEIHEYVLKNWRIVKIATTKAQRKLFFNLRKTKQRLGWFNAGEIEIVAKA
LNVTSKDVREMESRMSAQDMTFEQSSDEDNRGITIAPILYLQDKIVSSNEEEEWIDQVSDKLNAAIKTLDERSQHIIRAR
WLNVDNKSTLQELANQYGVSAERVRQLEKNAMKKMKLIIQN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=71.4285714285714, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789098, Length=305, Percent_Identity=26.2295081967213, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789448, Length=260, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32614; Mature: 32483

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYIQKNVTLIPQGNLEAYIKVANAYTMLTAEEEKTLAKRLYYNGDLDAAKKLIVSHLR
CCCCCCCCEEEEECCCCEEEEEEECCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FVVHVSRNYSGYGLPQADLIQEGNIGLMKAVRRFNPELDVRLVSFAVHWIKSEIHEYVLK
HHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKIATTKAQRKLFFNLRKTKQRLGWFNAGEIEIVAKALNVTSKDVREMESRMSAQD
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
MTFEQSSDEDNRGITIAPILYLQDKIVSSNEEEEWIDQVSDKLNAAIKTLDERSQHIIRA
CCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWLNVDNKSTLQELANQYGVSAERVRQLEKNAMKKMKLIIQN
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKYIQKNVTLIPQGNLEAYIKVANAYTMLTAEEEKTLAKRLYYNGDLDAAKKLIVSHLR
CCCCCCCEEEEECCCCEEEEEEECCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FVVHVSRNYSGYGLPQADLIQEGNIGLMKAVRRFNPELDVRLVSFAVHWIKSEIHEYVLK
HHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKIATTKAQRKLFFNLRKTKQRLGWFNAGEIEIVAKALNVTSKDVREMESRMSAQD
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
MTFEQSSDEDNRGITIAPILYLQDKIVSSNEEEEWIDQVSDKLNAAIKTLDERSQHIIRA
CCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWLNVDNKSTLQELANQYGVSAERVRQLEKNAMKKMKLIIQN
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]