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The map label for this gene is smpB [H]

Identifier: 71892321

GI number: 71892321

Start: 677490

End: 677954

Strand: Direct

Name: smpB [H]

Synonym: BPEN_568

Alternate gene names: 71892321

Gene position: 677490-677954 (Clockwise)

Preceding gene: 71892319

Following gene: 71892322

Centisome position: 85.58

GC content: 22.8

Gene sequence:

>465_bases
ATGAATCAGTTAATTTTAAAGAAAATTACACAAAATAAACATGCATATCATGAATATTTTATTGAAAAAAAAATTGAATC
TGGAATTTCATTGTTAGGATGGGAAGTAAAATCTGCTCGTTCTAATATGGTAACTATTAATAATAGTTACGTATCTTTTT
ATAATAAGGAAGCGTATATTTGTAATTCTATATTTCAATCAAATGTAACTCAATTTTATAATGAAACTCACAATTCCGTT
CGTGTGCGTAAATTGTTATTAAAAAAAAATGAATTATTATTTTTAATGGAAAAAGTTAATCAAAAAGGTTACACTGCAAT
TATTTTAGATTTGTATTGGAAAAATTCTTGGATTAAAGTGAATATTGGCCTTGCTAAAGGTAAAAAAAAATACGACAAAA
GAAATATAATACATATGCATAAATGGAAGAAAGAAAAAATACGTATTTTAAAGTATATGAAATAA

Upstream 100 bases:

>100_bases
AAGCCATATCAATCCTGTTTTGAATGGGTTTAAATATTACATACATATTAATATAATGGTTTTTAGTAATAAAAAATAAT
ATTTTTAAAAAAAAATTATT

Downstream 100 bases:

>100_bases
AAATTATTTGTATATACAGTATCCCGATGATATAATTGTTTTCGATGTTAGAATGGGGCTGATTTTGGATTCGACGGAGT
ATCTTGTTATTTAAAGTGCA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MNQLILKKITQNKHAYHEYFIEKKIESGISLLGWEVKSARSNMVTINNSYVSFYNKEAYICNSIFQSNVTQFYNETHNSV
RVRKLLLKKNELLFLMEKVNQKGYTAIILDLYWKNSWIKVNIGLAKGKKKYDKRNIIHMHKWKKEKIRILKYMK

Sequences:

>Translated_154_residues
MNQLILKKITQNKHAYHEYFIEKKIESGISLLGWEVKSARSNMVTINNSYVSFYNKEAYICNSIFQSNVTQFYNETHNSV
RVRKLLLKKNELLFLMEKVNQKGYTAIILDLYWKNSWIKVNIGLAKGKKKYDKRNIIHMHKWKKEKIRILKYMK
>Mature_154_residues
MNQLILKKITQNKHAYHEYFIEKKIESGISLLGWEVKSARSNMVTINNSYVSFYNKEAYICNSIFQSNVTQFYNETHNSV
RVRKLLLKKNELLFLMEKVNQKGYTAIILDLYWKNSWIKVNIGLAKGKKKYDKRNIIHMHKWKKEKIRILKYMK

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=47.6190476190476, Blast_Score=135, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18510; Mature: 18510

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLILKKITQNKHAYHEYFIEKKIESGISLLGWEVKSARSNMVTINNSYVSFYNKEAYI
CCHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEEEHHCCCCCEEEEECCEEEEECCCHHH
CNSIFQSNVTQFYNETHNSVRVRKLLLKKNELLFLMEKVNQKGYTAIILDLYWKNSWIKV
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEECCCEEEE
NIGLAKGKKKYDKRNIIHMHKWKKEKIRILKYMK
EEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQLILKKITQNKHAYHEYFIEKKIESGISLLGWEVKSARSNMVTINNSYVSFYNKEAYI
CCHHHHHHHHCCCHHHHHHHHHHHHHCCHHEEEEEHHCCCCCEEEEECCEEEEECCCHHH
CNSIFQSNVTQFYNETHNSVRVRKLLLKKNELLFLMEKVNQKGYTAIILDLYWKNSWIKV
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEECCCEEEE
NIGLAKGKKKYDKRNIIHMHKWKKEKIRILKYMK
EEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA