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The map label for this gene is yfgB

Identifier: 71892305

GI number: 71892305

Start: 660713

End: 661834

Strand: Reverse

Name: yfgB

Synonym: BPEN_552

Alternate gene names: 71892305

Gene position: 661834-660713 (Counterclockwise)

Preceding gene: 71892306

Following gene: 71892304

Centisome position: 83.6

GC content: 31.11

Gene sequence:

>1122_bases
TTGGATCTTGAGAAGTATATCTTTATGTATGCAAAAAAGGTTAATTTACTTAACATGAACAAAGAAGAATTATTAATTTT
TTTTGATAAATTAGGTGAAAAACCTTTTCGTTCTCATCAAATAATGAGATGGATATATCATTATTATTGTGATGATTTTA
ATTACATGACAAATATAAGTAAGTCTCTAAAAGAGAGATTGAAACAAATAGCGGAAATCCGTGCGCCAATCATTATAAAA
GAACAACTATCTTCAGATGGAACAATTAAATGGGCTATGAAAATTGATGAACAACAAATCGAAACAGTTTATATTCCTGA
AAATAAGAGAACCACTTTGTGTGTTTCATCACAAATTGGATGCCCTTTAGGATGTAGTTTTTGTGGAACAGCGCAACAGG
GATTTAACAGAAATCTGAATGTATCGGAGATAATTGGTCAAGTTTGGAGAGCAGCGCAACTTATCAATCTTAATAAAAAA
ATAAAAATAAAAAATAATAGATTTCCTATTACTAATATAGTGTTTATGGGTATGGGAGAGCCATTATTAAATATTGTTAA
CGTTGTATCGGCGATCAGAATTATATTAGATGACTTTGGTTTTAAGTTATCGAAACGACATATTACGCTCTCAACTGCTG
GGATCGTACCAGGTATAGAAAAATTAAAAAATATGATTGACATCCCATTAGCTATATCTCTACATGCTCCAAATGATATC
ATTAGAAATAAGATTATGCCAATTAATAAAAAGTACAATATTAATAGCGTATTAGAAGCAGCGCGTAGGTATTCGATGGA
CACTAAATCTAATCACGGTAGAATAACAATAGAGTATGTATTGTTAAAAAATATTAATGACGATGTCCTGCACGCCCATC
AATTAGCAAAACAACTTCAAGGTATTCCGTGTAAAATTAACTTGATCCCATGGAACCCAATTCCGAATATACGATATGCA
TGTAGCTCACAGATTCGTATGCGTGCATTTCTTAAAGTTTTGTTGAAATATAATATAGTAACCATCATACGTAAAATAAG
AGGAGCGGACATTAATGCTGCCTGTGGACAATTAACAGGAGAAGTAATTAACCGTATAAATTTACAATATAACTCGTTAT
AA

Upstream 100 bases:

>100_bases
AAATACAGATCAATTTAAGTCAAAAAGAATATCGGTAATATAGTCAATTTCTATTGAGCGAATGCTCATAGAGCATTACT
GTTGATCAACTTTCGTAAAA

Downstream 100 bases:

>100_bases
ACGATATCATTGTATTTAATATTATTATAAGATTAATAAGACGGAATTTTTTAAAAAAGGGTCATAAATGTAATATGACA
ATTACGCATATTGCTATGTA

Product: putative pyruvate formate lyase activating enzyme 2

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MDLEKYIFMYAKKVNLLNMNKEELLIFFDKLGEKPFRSHQIMRWIYHYYCDDFNYMTNISKSLKERLKQIAEIRAPIIIK
EQLSSDGTIKWAMKIDEQQIETVYIPENKRTTLCVSSQIGCPLGCSFCGTAQQGFNRNLNVSEIIGQVWRAAQLINLNKK
IKIKNNRFPITNIVFMGMGEPLLNIVNVVSAIRIILDDFGFKLSKRHITLSTAGIVPGIEKLKNMIDIPLAISLHAPNDI
IRNKIMPINKKYNINSVLEAARRYSMDTKSNHGRITIEYVLLKNINDDVLHAHQLAKQLQGIPCKINLIPWNPIPNIRYA
CSSQIRMRAFLKVLLKYNIVTIIRKIRGADINAACGQLTGEVINRINLQYNSL

Sequences:

>Translated_373_residues
MDLEKYIFMYAKKVNLLNMNKEELLIFFDKLGEKPFRSHQIMRWIYHYYCDDFNYMTNISKSLKERLKQIAEIRAPIIIK
EQLSSDGTIKWAMKIDEQQIETVYIPENKRTTLCVSSQIGCPLGCSFCGTAQQGFNRNLNVSEIIGQVWRAAQLINLNKK
IKIKNNRFPITNIVFMGMGEPLLNIVNVVSAIRIILDDFGFKLSKRHITLSTAGIVPGIEKLKNMIDIPLAISLHAPNDI
IRNKIMPINKKYNINSVLEAARRYSMDTKSNHGRITIEYVLLKNINDDVLHAHQLAKQLQGIPCKINLIPWNPIPNIRYA
CSSQIRMRAFLKVLLKYNIVTIIRKIRGADINAACGQLTGEVINRINLQYNSL
>Mature_373_residues
MDLEKYIFMYAKKVNLLNMNKEELLIFFDKLGEKPFRSHQIMRWIYHYYCDDFNYMTNISKSLKERLKQIAEIRAPIIIK
EQLSSDGTIKWAMKIDEQQIETVYIPENKRTTLCVSSQIGCPLGCSFCGTAQQGFNRNLNVSEIIGQVWRAAQLINLNKK
IKIKNNRFPITNIVFMGMGEPLLNIVNVVSAIRIILDDFGFKLSKRHITLSTAGIVPGIEKLKNMIDIPLAISLHAPNDI
IRNKIMPINKKYNINSVLEAARRYSMDTKSNHGRITIEYVLLKNINDDVLHAHQLAKQLQGIPCKINLIPWNPIPNIRYA
CSSQIRMRAFLKVLLKYNIVTIIRKIRGADINAACGQLTGEVINRINLQYNSL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=353, Percent_Identity=61.4730878186969, Blast_Score=477, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BLOPB (Q492D9)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278039.1
- ProteinModelPortal:   Q492D9
- STRING:   Q492D9
- GeneID:   3562613
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_552
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- BioCyc:   CBLO291272:BPEN_552-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42864; Mature: 42864

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLEKYIFMYAKKVNLLNMNKEELLIFFDKLGEKPFRSHQIMRWIYHYYCDDFNYMTNIS
CCHHHHHHHHHHHHHEECCCHHHHEEEEHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
KSLKERLKQIAEIRAPIIIKEQLSSDGTIKWAMKIDEQQIETVYIPENKRTTLCVSSQIG
HHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCCCCEEEECCCCCEEEEEECCCC
CPLGCSFCGTAQQGFNRNLNVSEIIGQVWRAAQLINLNKKIKIKNNRFPITNIVFMGMGE
CCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEEEECCCH
PLLNIVNVVSAIRIILDDFGFKLSKRHITLSTAGIVPGIEKLKNMIDIPLAISLHAPNDI
HHHHHHHHHHHHHHHHHHHCCEEECCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHH
IRNKIMPINKKYNINSVLEAARRYSMDTKSNHGRITIEYVLLKNINDDVLHAHQLAKQLQ
HHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHC
GIPCKINLIPWNPIPNIRYACSSQIRMRAFLKVLLKYNIVTIIRKIRGADINAACGQLTG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVINRINLQYNSL
HHHHHHCCEECCC
>Mature Secondary Structure
MDLEKYIFMYAKKVNLLNMNKEELLIFFDKLGEKPFRSHQIMRWIYHYYCDDFNYMTNIS
CCHHHHHHHHHHHHHEECCCHHHHEEEEHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
KSLKERLKQIAEIRAPIIIKEQLSSDGTIKWAMKIDEQQIETVYIPENKRTTLCVSSQIG
HHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCCCCEEEECCCCCEEEEEECCCC
CPLGCSFCGTAQQGFNRNLNVSEIIGQVWRAAQLINLNKKIKIKNNRFPITNIVFMGMGE
CCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEEEECCCH
PLLNIVNVVSAIRIILDDFGFKLSKRHITLSTAGIVPGIEKLKNMIDIPLAISLHAPNDI
HHHHHHHHHHHHHHHHHHHCCEEECCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHH
IRNKIMPINKKYNINSVLEAARRYSMDTKSNHGRITIEYVLLKNINDDVLHAHQLAKQLQ
HHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHC
GIPCKINLIPWNPIPNIRYACSSQIRMRAFLKVLLKYNIVTIIRKIRGADINAACGQLTG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVINRINLQYNSL
HHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA